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The map label for this gene is ppa [H]

Identifier: 88658186

GI number: 88658186

Start: 1040474

End: 1040992

Strand: Reverse

Name: ppa [H]

Synonym: ECH_1014

Alternate gene names: 88658186

Gene position: 1040992-1040474 (Counterclockwise)

Preceding gene: 88657913

Following gene: 88657833

Centisome position: 88.5

GC content: 31.02

Gene sequence:

>519_bases
ATGAATTTAGACAATGTGACTGGTGGTGATAATGTTCCTAAAGAAATCAATGTTATTATTGAAATTAGTCAGAATTCCTG
TCCGGTAAAGTATGAGTTTGATAAAGAGAAAAATTTATTTTGTGTAGATAGGTTTTTACCTACATCTATGTATTATCCAT
GTAATTATGGTTTTATTCCACATACTTGTGCTGGAGATGGTGATCCTGTTGATGTTTTGGTTGCATCTAGATTTCCTGTA
ATGTCTGGGGCGGTAATCCGCGCAAGGCCAGTAGGTGTTTTAGTTATGCATGATGAAAGTGGAGAAGATGTAAAAATATT
AGCTGTTCCTACACATAAAGTAGACCAATACTATAATAATATAAAAGATTATTCCGATTTTCCTGTATCTTTTTTAAATT
CAATATCCCATTTTTTTACTTTTTATAAAAAATTGGAGGAAGATAAATTTGTATCTGTGGAAGGTTGGAAAGATGTTACG
GTTGCAGAAAAATTGATATTAAGTGCTTTAATTAAATAA

Upstream 100 bases:

>100_bases
CATTGACTAGAATATAAAGGATATTAGTATATAAGCTGATGGTCATTTATTGATCTATTCAAAATTTTTTTTATTTGTTT
GTGATATCAAGAGATTAATT

Downstream 100 bases:

>100_bases
ATGTTGTAGTGTTAGGTTGTAATAGTGCTACAGTGCTTATATTTATAAGAAATTGATAATCTGGGAAGTACTACTGTAAG
CGATTTTTATTGTTGTAGTT

Product: inorganic pyrophosphatase

Products: NA

Alternate protein names: Pyrophosphate phospho-hydrolase; PPase [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MNLDNVTGGDNVPKEINVIIEISQNSCPVKYEFDKEKNLFCVDRFLPTSMYYPCNYGFIPHTCAGDGDPVDVLVASRFPV
MSGAVIRARPVGVLVMHDESGEDVKILAVPTHKVDQYYNNIKDYSDFPVSFLNSISHFFTFYKKLEEDKFVSVEGWKDVT
VAEKLILSALIK

Sequences:

>Translated_172_residues
MNLDNVTGGDNVPKEINVIIEISQNSCPVKYEFDKEKNLFCVDRFLPTSMYYPCNYGFIPHTCAGDGDPVDVLVASRFPV
MSGAVIRARPVGVLVMHDESGEDVKILAVPTHKVDQYYNNIKDYSDFPVSFLNSISHFFTFYKKLEEDKFVSVEGWKDVT
VAEKLILSALIK
>Mature_172_residues
MNLDNVTGGDNVPKEINVIIEISQNSCPVKYEFDKEKNLFCVDRFLPTSMYYPCNYGFIPHTCAGDGDPVDVLVASRFPV
MSGAVIRARPVGVLVMHDESGEDVKILAVPTHKVDQYYNNIKDYSDFPVSFLNSISHFFTFYKKLEEDKFVSVEGWKDVT
VAEKLILSALIK

Specific function: Unknown

COG id: COG0221

COG function: function code C; Inorganic pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790673, Length=170, Percent_Identity=51.1764705882353, Blast_Score=186, Evalue=9e-49,

Paralogues:

None

Copy number: 5480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008162 [H]

Pfam domain/function: PF00719 Pyrophosphatase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 19410; Mature: 19410

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00387 PPASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDNVTGGDNVPKEINVIIEISQNSCPVKYEFDKEKNLFCVDRFLPTSMYYPCNYGFIP
CCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCEECCCCCCCCC
HTCAGDGDPVDVLVASRFPVMSGAVIRARPVGVLVMHDESGEDVKILAVPTHKVDQYYNN
CCCCCCCCCEEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCEEEEEECCHHHHHHHHH
IKDYSDFPVSFLNSISHFFTFYKKLEEDKFVSVEGWKDVTVAEKLILSALIK
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLDNVTGGDNVPKEINVIIEISQNSCPVKYEFDKEKNLFCVDRFLPTSMYYPCNYGFIP
CCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEECCCCCCEECCCCCCCCC
HTCAGDGDPVDVLVASRFPVMSGAVIRARPVGVLVMHDESGEDVKILAVPTHKVDQYYNN
CCCCCCCCCEEEEEECCCCCCCCCEEEECCEEEEEEECCCCCCEEEEEECCHHHHHHHHH
IKDYSDFPVSFLNSISHFFTFYKKLEEDKFVSVEGWKDVTVAEKLILSALIK
HCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]