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The map label for this gene is nifS1 [H]

Identifier: 88658169

GI number: 88658169

Start: 635089

End: 636567

Strand: Direct

Name: nifS1 [H]

Synonym: ECH_0628

Alternate gene names: 88658169

Gene position: 635089-636567 (Clockwise)

Preceding gene: 88658037

Following gene: 88658103

Centisome position: 53.99

GC content: 28.94

Gene sequence:

>1479_bases
ATGTCACATATTGCAAATAGCCAATCCCTCTCTGAAGGATACTTAGAGCAAATTATTGTAAAACTAAAAAAGCAAGGATT
AATAAACTCTACAAAAGGCCCTGGTGGTGGTTATTCTTTAAATAAGAGTCCAAATTCAATTACACTTAATCTAATTCTTG
AATCCATAGGAGAAAATATAAAAATTACAAGATGTAAAAATCATCTCATAGGATGCTTATCAAATAATGCTAGATGTATT
ACTCACAATTTATGGGATAATATAGGCAATCATATTAAAAAATATTTAAATAGCATTTCATTAGAAGATATACTTAACAA
TAATTTTAAATCTGGCACGACTGTAAACAATAATGCTAATGAATATATATATGCAGATTATAATTCAACATCAACTATAT
TACCCACAGTTAAAAGTCAATTAGATAATCTATCTTCTCTAAACATATACAATCCGTCATCAACACATAAACTAGGGCAA
AATACAAAAAGTATTATAGAAAAAACAAGAGAAATAGCCATCAATCAATTAAATGCTAAAAATCATGACGTAATTTTTAC
TTCATCTGGTACAGAAGCAAACAATTTAGTAATAAATAGTACTGCTGATTATAAATACTTAATTTCTTCCATAGAACATC
TATCTATCATGAACTGTGCAATAAATGCAGAATTAATACCAGTAGATTCTAATGGAACGGTATGCTTAGATACATTAAGC
GATATTTTATACAAATGTAAAGATGAAAAAGTTCTCGTATCAATAATGACTGCAAATAATGAAACTGGAGTAATTCAACC
TATAAAAGAAATAGTAGAAATATCACATAAATTTGGTGCAATAGTACATACAGATGCTATACAAGCATGCGGAAAAATTC
ACGTAGATATTGAAGATTTAGGGGTAGATTTACTAACAATTTCATCACATAAACTTGGTAGTATTGCTGGAACTGGTATA
TTGTTTTTTAATAGTAAAAAAATAAAAATAAAGCCTATGATATTAGGTGGACACCAAGAAAAAGGACTACGTGCAGGTAC
TGAAAATGTTGTATCAATCTATTTATTATCTATATCTCTAAGCAATCTAAAAGATTCTATAAAAAAAATGTCTAGTGTTG
AGAGATTACGTGATAAACTAGAAAATCAAATATTAAACTTGGTACCTGAAGCTCAAATTTTTGGCAAGAACACACGAAGG
TTACCAAACACTACATGCATTTCGATGCCAAATGTAAACAGTGAAATACAAACAATAAGTTTTGATATTGATAACATTGC
AGTTGGTAGTGGATCTGCCTGTTCTTCTGGAGCATTAGAACATTCCCATGTATTAGCTGCGATGGGAATAGATGATAACG
TAGCTAAAAACTCTATCAGGATTAGTCTTAGTCCTGATGTAACAGATGATCAAGTAAACAAAATAGTTAATTGTTGGTAC
AAGATATATAAAAATAATCAATTACTCAAATTAAATTAG

Upstream 100 bases:

>100_bases
TAATATGTTAATTACGACAAGGCTACGTTATGCTGTCATGTTTATGGTAAAATTAGCTCAGGAGTATTACACATTAAAAG
GTAGCACTCAGCCAAAAAGG

Downstream 100 bases:

>100_bases
GTAGAAGTGATGAAAAAATTAGAAGATTATTATATTATGAGTAGTAGGTCATAAGATGGAACAAGAAAAACGACAAATCA
ATTTACCTGTGTTTCTCGAC

Product: rrf2/aminotransferase, class V family protein

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MSHIANSQSLSEGYLEQIIVKLKKQGLINSTKGPGGGYSLNKSPNSITLNLILESIGENIKITRCKNHLIGCLSNNARCI
THNLWDNIGNHIKKYLNSISLEDILNNNFKSGTTVNNNANEYIYADYNSTSTILPTVKSQLDNLSSLNIYNPSSTHKLGQ
NTKSIIEKTREIAINQLNAKNHDVIFTSSGTEANNLVINSTADYKYLISSIEHLSIMNCAINAELIPVDSNGTVCLDTLS
DILYKCKDEKVLVSIMTANNETGVIQPIKEIVEISHKFGAIVHTDAIQACGKIHVDIEDLGVDLLTISSHKLGSIAGTGI
LFFNSKKIKIKPMILGGHQEKGLRAGTENVVSIYLLSISLSNLKDSIKKMSSVERLRDKLENQILNLVPEAQIFGKNTRR
LPNTTCISMPNVNSEIQTISFDIDNIAVGSGSACSSGALEHSHVLAAMGIDDNVAKNSIRISLSPDVTDDQVNKIVNCWY
KIYKNNQLLKLN

Sequences:

>Translated_492_residues
MSHIANSQSLSEGYLEQIIVKLKKQGLINSTKGPGGGYSLNKSPNSITLNLILESIGENIKITRCKNHLIGCLSNNARCI
THNLWDNIGNHIKKYLNSISLEDILNNNFKSGTTVNNNANEYIYADYNSTSTILPTVKSQLDNLSSLNIYNPSSTHKLGQ
NTKSIIEKTREIAINQLNAKNHDVIFTSSGTEANNLVINSTADYKYLISSIEHLSIMNCAINAELIPVDSNGTVCLDTLS
DILYKCKDEKVLVSIMTANNETGVIQPIKEIVEISHKFGAIVHTDAIQACGKIHVDIEDLGVDLLTISSHKLGSIAGTGI
LFFNSKKIKIKPMILGGHQEKGLRAGTENVVSIYLLSISLSNLKDSIKKMSSVERLRDKLENQILNLVPEAQIFGKNTRR
LPNTTCISMPNVNSEIQTISFDIDNIAVGSGSACSSGALEHSHVLAAMGIDDNVAKNSIRISLSPDVTDDQVNKIVNCWY
KIYKNNQLLKLN
>Mature_491_residues
SHIANSQSLSEGYLEQIIVKLKKQGLINSTKGPGGGYSLNKSPNSITLNLILESIGENIKITRCKNHLIGCLSNNARCIT
HNLWDNIGNHIKKYLNSISLEDILNNNFKSGTTVNNNANEYIYADYNSTSTILPTVKSQLDNLSSLNIYNPSSTHKLGQN
TKSIIEKTREIAINQLNAKNHDVIFTSSGTEANNLVINSTADYKYLISSIEHLSIMNCAINAELIPVDSNGTVCLDTLSD
ILYKCKDEKVLVSIMTANNETGVIQPIKEIVEISHKFGAIVHTDAIQACGKIHVDIEDLGVDLLTISSHKLGSIAGTGIL
FFNSKKIKIKPMILGGHQEKGLRAGTENVVSIYLLSISLSNLKDSIKKMSSVERLRDKLENQILNLVPEAQIFGKNTRRL
PNTTCISMPNVNSEIQTISFDIDNIAVGSGSACSSGALEHSHVLAAMGIDDNVAKNSIRISLSPDVTDDQVNKIVNCWYK
IYKNNQLLKLN

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=374, Percent_Identity=31.0160427807487, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI156713448, Length=422, Percent_Identity=31.2796208530806, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI48994898, Length=370, Percent_Identity=31.6216216216216, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1788880, Length=107, Percent_Identity=34.5794392523364, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787970, Length=198, Percent_Identity=32.3232323232323, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=399, Percent_Identity=32.0802005012531, Blast_Score=187, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17533177, Length=321, Percent_Identity=31.1526479750779, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6319831, Length=373, Percent_Identity=33.2439678284182, Blast_Score=191, Evalue=3e-49,
Organism=Drosophila melanogaster, GI20129463, Length=371, Percent_Identity=31.5363881401617, Blast_Score=177, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 53876; Mature: 53745

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS01332 UPF0074

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHIANSQSLSEGYLEQIIVKLKKQGLINSTKGPGGGYSLNKSPNSITLNLILESIGENI
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCE
KITRCKNHLIGCLSNNARCITHNLWDNIGNHIKKYLNSISLEDILNNNFKSGTTVNNNAN
EEEEECCHHEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEECCCCC
EYIYADYNSTSTILPTVKSQLDNLSSLNIYNPSSTHKLGQNTKSIIEKTREIAINQLNAK
EEEEEECCCCCEEHHHHHHHHCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCC
NHDVIFTSSGTEANNLVINSTADYKYLISSIEHLSIMNCAINAELIPVDSNGTVCLDTLS
CCCEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEECCEEEEECCCCCEEHHHHH
DILYKCKDEKVLVSIMTANNETGVIQPIKEIVEISHKFGAIVHTDAIQACGKIHVDIEDL
HHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHCCEEEEEHHHC
GVDLLTISSHKLGSIAGTGILFFNSKKIKIKPMILGGHQEKGLRAGTENVVSIYLLSISL
CEEEEEECCCCCCCEECCEEEEEECCEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEH
SNLKDSIKKMSSVERLRDKLENQILNLVPEAQIFGKNTRRLPNTTCISMPNVNSEIQTIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEECCCCCCCEEEEE
FDIDNIAVGSGSACSSGALEHSHVLAAMGIDDNVAKNSIRISLSPDVTDDQVNKIVNCWY
EECCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KIYKNNQLLKLN
HHHCCCEEEEEC
>Mature Secondary Structure 
SHIANSQSLSEGYLEQIIVKLKKQGLINSTKGPGGGYSLNKSPNSITLNLILESIGENI
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHCCCE
KITRCKNHLIGCLSNNARCITHNLWDNIGNHIKKYLNSISLEDILNNNFKSGTTVNNNAN
EEEEECCHHEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCEECCCCC
EYIYADYNSTSTILPTVKSQLDNLSSLNIYNPSSTHKLGQNTKSIIEKTREIAINQLNAK
EEEEEECCCCCEEHHHHHHHHCCCCEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHCCCC
NHDVIFTSSGTEANNLVINSTADYKYLISSIEHLSIMNCAINAELIPVDSNGTVCLDTLS
CCCEEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHEEEEECCEEEEECCCCCEEHHHHH
DILYKCKDEKVLVSIMTANNETGVIQPIKEIVEISHKFGAIVHTDAIQACGKIHVDIEDL
HHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEHHHHHHCCEEEEEHHHC
GVDLLTISSHKLGSIAGTGILFFNSKKIKIKPMILGGHQEKGLRAGTENVVSIYLLSISL
CEEEEEECCCCCCCEECCEEEEEECCEEEEEEEEECCCCCCCCCCCCCCEEEEEEEEEEH
SNLKDSIKKMSSVERLRDKLENQILNLVPEAQIFGKNTRRLPNTTCISMPNVNSEIQTIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEECCCCCCCEEEEE
FDIDNIAVGSGSACSSGALEHSHVLAAMGIDDNVAKNSIRISLSPDVTDDQVNKIVNCWY
EECCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
KIYKNNQLLKLN
HHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]