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The map label for this gene is iscS

Identifier: 88658103

GI number: 88658103

Start: 636623

End: 637855

Strand: Direct

Name: iscS

Synonym: ECH_0629

Alternate gene names: 88658103

Gene position: 636623-637855 (Clockwise)

Preceding gene: 88658169

Following gene: 88658479

Centisome position: 54.12

GC content: 34.87

Gene sequence:

>1233_bases
ATGGAACAAGAAAAACGACAAATCAATTTACCTGTGTTTCTCGACTATCAATCTACAACAAAAACAGATGATAGGGTATT
AGAAGCTATGATGCCCTACTTTAAACAATTTTCTAATCCTCACTCACGCAGTCACTCCTTTGGCTGGAAAGCTGAATCAG
CAGTTGAGTTAGCCAGGGAAAGAGTCGCATCTTTAATAAATGCCGAAGCTAAAGAAGTAATATTCACTTCAGGTGCAACA
GAATCAAATAACTTAGCAATTAAAGGAGTAGCAAACTTTTATAAAAACAAAGGAAATCATATAATTACAGTACGTACAGA
ACATAAATGCGTTTTAGATTCATGCCGTTACTTAGAGACAGAAGGGTTTCATGTTACTTACTTAGACGTACAAAAAAACG
GTATCTTAGATTTAGAGTTATTAAAATCATCTATCACTGATAAAACAATACTAGTATCAGTAATGATGGTGAACAATGAA
ATTGGCGTTATTCAACCAATTGAAAAAATAGGAAAAATTTGTCATGAACATGGAATATTTTTTCATACTGATGCAGCTCA
AGCTTTTGGAAAAATATCAATAGATGTCAAAAAAATGAACATCGATCTTTTAAGTATATCAGGACACAAGATATATGCTC
CAATGGGAATAGGAGCACTATATATACGCAAACGCCAACCACGAGTACGCCTTACTCCTATGATTAACGGAGGTGGTCAA
GAGCGTGGTATGAGATCAGGAACAGTACCTACTCCATTAGCTGTAGGGTTAGGAGAAGCAGCACGTATAGCTCAAGAAGT
AATGGAGGAAGAAAACATCAGGATAAGAGAATTGCGAGACATTTTATATAATGAAATAAAAAAACACTTACCATATGTCG
TATTAAACGGGGATTACGAACAACGTATAGCAGGAAATCTAAATTTAAGTTTTCCATATGTTGAAGGAGAATCTATAATT
ATGGCAATCAATAATCTCGCAGTCAGTTCAGGTTCTGCTTGTACTTCTGCTTCTTTAGAACCATCCTATGTTTTACGTGC
TTTAAATATTGAAAAAGACTTAGAGCATTCATCCATCAGATTTGGTATAGGTAGGTTTACTACAAGAGAAGAAATTTTAT
ATGCCGCAGAGCTTATTGTTAGCAGCATAAAAAAATTACGTGAGATGAGTCCATTATGGGAAATGGTCCAAGAAGGTGTA
GACCTTAATAATATCAAATGGGATGCACATTGA

Upstream 100 bases:

>100_bases
TGGTACAAGATATATAAAAATAATCAATTACTCAAATTAAATTAGGTAGAAGTGATGAAAAAATTAGAAGATTATTATAT
TATGAGTAGTAGGTCATAAG

Downstream 100 bases:

>100_bases
TTATGTTATTATATTGTCGTAATTATGAGGTAATTTTATGAGTTATAGTGAATCTCTTCTAGAGCATTACAAAAATCCTA
AAAACGTCGGTACTCTACCT

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MEQEKRQINLPVFLDYQSTTKTDDRVLEAMMPYFKQFSNPHSRSHSFGWKAESAVELARERVASLINAEAKEVIFTSGAT
ESNNLAIKGVANFYKNKGNHIITVRTEHKCVLDSCRYLETEGFHVTYLDVQKNGILDLELLKSSITDKTILVSVMMVNNE
IGVIQPIEKIGKICHEHGIFFHTDAAQAFGKISIDVKKMNIDLLSISGHKIYAPMGIGALYIRKRQPRVRLTPMINGGGQ
ERGMRSGTVPTPLAVGLGEAARIAQEVMEEENIRIRELRDILYNEIKKHLPYVVLNGDYEQRIAGNLNLSFPYVEGESII
MAINNLAVSSGSACTSASLEPSYVLRALNIEKDLEHSSIRFGIGRFTTREEILYAAELIVSSIKKLREMSPLWEMVQEGV
DLNNIKWDAH

Sequences:

>Translated_410_residues
MEQEKRQINLPVFLDYQSTTKTDDRVLEAMMPYFKQFSNPHSRSHSFGWKAESAVELARERVASLINAEAKEVIFTSGAT
ESNNLAIKGVANFYKNKGNHIITVRTEHKCVLDSCRYLETEGFHVTYLDVQKNGILDLELLKSSITDKTILVSVMMVNNE
IGVIQPIEKIGKICHEHGIFFHTDAAQAFGKISIDVKKMNIDLLSISGHKIYAPMGIGALYIRKRQPRVRLTPMINGGGQ
ERGMRSGTVPTPLAVGLGEAARIAQEVMEEENIRIRELRDILYNEIKKHLPYVVLNGDYEQRIAGNLNLSFPYVEGESII
MAINNLAVSSGSACTSASLEPSYVLRALNIEKDLEHSSIRFGIGRFTTREEILYAAELIVSSIKKLREMSPLWEMVQEGV
DLNNIKWDAH
>Mature_410_residues
MEQEKRQINLPVFLDYQSTTKTDDRVLEAMMPYFKQFSNPHSRSHSFGWKAESAVELARERVASLINAEAKEVIFTSGAT
ESNNLAIKGVANFYKNKGNHIITVRTEHKCVLDSCRYLETEGFHVTYLDVQKNGILDLELLKSSITDKTILVSVMMVNNE
IGVIQPIEKIGKICHEHGIFFHTDAAQAFGKISIDVKKMNIDLLSISGHKIYAPMGIGALYIRKRQPRVRLTPMINGGGQ
ERGMRSGTVPTPLAVGLGEAARIAQEVMEEENIRIRELRDILYNEIKKHLPYVVLNGDYEQRIAGNLNLSFPYVEGESII
MAINNLAVSSGSACTSASLEPSYVLRALNIEKDLEHSSIRFGIGRFTTREEILYAAELIVSSIKKLREMSPLWEMVQEGV
DLNNIKWDAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=401, Percent_Identity=62.8428927680798, Blast_Score=539, Evalue=1e-153,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=34.0632603406326, Blast_Score=205, Evalue=7e-53,
Organism=Escherichia coli, GI48994898, Length=406, Percent_Identity=59.6059113300493, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1787970, Length=240, Percent_Identity=29.1666666666667, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789175, Length=230, Percent_Identity=28.2608695652174, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25143064, Length=402, Percent_Identity=61.1940298507463, Blast_Score=525, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17533177, Length=320, Percent_Identity=33.125, Blast_Score=154, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=58.1047381546135, Blast_Score=491, Evalue=1e-139,
Organism=Drosophila melanogaster, GI20129463, Length=401, Percent_Identity=62.3441396508728, Blast_Score=535, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_EHRCR (Q2GGJ4)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507439.1
- ProteinModelPortal:   Q2GGJ4
- SMR:   Q2GGJ4
- STRING:   Q2GGJ4
- GeneID:   3927819
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0629
- TIGR:   ECH_0629
- eggNOG:   COG1104
- HOGENOM:   HBG635316
- OMA:   SYVLRSM
- PhylomeDB:   Q2GGJ4
- ProtClustDB:   PRK14012
- BioCyc:   ECHA205920:ECH_0629-MONOMER
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 46098; Mature: 46098

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 334-334

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQEKRQINLPVFLDYQSTTKTDDRVLEAMMPYFKQFSNPHSRSHSFGWKAESAVELARE
CCCCHHEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
RVASLINAEAKEVIFTSGATESNNLAIKGVANFYKNKGNHIITVRTEHKCVLDSCRYLET
HHHHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCCHHHHHHHHHEEC
EGFHVTYLDVQKNGILDLELLKSSITDKTILVSVMMVNNEIGVIQPIEKIGKICHEHGIF
CCCEEEEEEECCCCCEEHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCE
FHTDAAQAFGKISIDVKKMNIDLLSISGHKIYAPMGIGALYIRKRQPRVRLTPMINGGGQ
EEECHHHHCCEEEEEEEEEEEEEEEECCCEEEECCCCCEEEEECCCCCEEEEEEECCCCH
ERGMRSGTVPTPLAVGLGEAARIAQEVMEEENIRIRELRDILYNEIKKHLPYVVLNGDYE
HCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCHH
QRIAGNLNLSFPYVEGESIIMAINNLAVSSGSACTSASLEPSYVLRALNIEKDLEHSSIR
HHHCCCCEEEECEECCCEEEEEEEEEEECCCCCCCCCCCCHHHHHEECCCHHCCCCCCEE
FGIGRFTTREEILYAAELIVSSIKKLREMSPLWEMVQEGVDLNNIKWDAH
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEECCC
>Mature Secondary Structure
MEQEKRQINLPVFLDYQSTTKTDDRVLEAMMPYFKQFSNPHSRSHSFGWKAESAVELARE
CCCCHHEECCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
RVASLINAEAKEVIFTSGATESNNLAIKGVANFYKNKGNHIITVRTEHKCVLDSCRYLET
HHHHHHCCCCCEEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCCHHHHHHHHHEEC
EGFHVTYLDVQKNGILDLELLKSSITDKTILVSVMMVNNEIGVIQPIEKIGKICHEHGIF
CCCEEEEEEECCCCCEEHHHHHHHCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCE
FHTDAAQAFGKISIDVKKMNIDLLSISGHKIYAPMGIGALYIRKRQPRVRLTPMINGGGQ
EEECHHHHCCEEEEEEEEEEEEEEEECCCEEEECCCCCEEEEECCCCCEEEEEEECCCCH
ERGMRSGTVPTPLAVGLGEAARIAQEVMEEENIRIRELRDILYNEIKKHLPYVVLNGDYE
HCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECCCHH
QRIAGNLNLSFPYVEGESIIMAINNLAVSSGSACTSASLEPSYVLRALNIEKDLEHSSIR
HHHCCCCEEEECEECCCEEEEEEEEEEECCCCCCCCCCCCHHHHHEECCCHHCCCCCCEE
FGIGRFTTREEILYAAELIVSSIKKLREMSPLWEMVQEGVDLNNIKWDAH
EECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA