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The map label for this gene is truA

Identifier: 88658037

GI number: 88658037

Start: 629795

End: 630532

Strand: Direct

Name: truA

Synonym: ECH_0622

Alternate gene names: 88658037

Gene position: 629795-630532 (Clockwise)

Preceding gene: 88658464

Following gene: 88658169

Centisome position: 53.54

GC content: 27.64

Gene sequence:

>738_bases
ATGCGATATAAAATTACTATAGAATATAATGGATCTAATTTTATTGGATGGCAAAAACAAAAACACTCATCAAATTCCAT
TCAAGAAATTCTTGAAAATGCAATACTCAAATTTTCACAACAACATACTACAGTATATGTTGCGGGAAGGACTGATGCTG
GTGTACATGCTTTAGGTCAAGTAGCACATTTTGATTTAACAACAAACCTTGACACATATGTAGTAAGAAATGCAATAAAT
TATCACTTAATACCTCATGCAATTGCCATATTAAGTGTAGAAAAAACAGATGATAAATTTCATGCAAGATTTTCAGCAAA
AAAACGTCACTATTTATACAAAATAATAAATCGTTATTCACCATTAACAATAGATTATAATCGTGCGTGGCTTATACATA
ATCCTTTAAATATTGAAAATATGATACAAGCTATCGAATATATAAAAGGTAATCATGATTTCTCTAGTTTCAGAGCAAGG
CATTGTCAATCCAAAAATCCAATAAAAACAATAGATGATTTAAAAATTATTCATAATAATCAATCTATTAACATTCATAT
TTCTGCTATATCATTTTTACATCATCAAGTACGTATTATTGTAGGTACACTAGTTGAGTGTGGAAAAAATAATTTTTCAC
CAGAACATATAAAAAATATCTTAAAAGCAAAAAATAGATCTTATGCAGGTATGACAGCACCACCATACGGCTTATATTTT
GTAAAAGTAGATTATTAA

Upstream 100 bases:

>100_bases
AAAAAAATTCTGTAATTCTATTACAAGTAAAAATGGGTGTTGCAGCACGTAAAGCTATATTACATTATTTACTGTACAAT
AAGTCAATATAACATCAATA

Downstream 100 bases:

>100_bases
AGTTTTATACAGTACAAAACGTACTTTCGATAAATAAAAATTCACTAATAATGCTTAAGTAAATAACAGTATAAATACAT
ATGGTTAAACAGCATATCTA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MRYKITIEYNGSNFIGWQKQKHSSNSIQEILENAILKFSQQHTTVYVAGRTDAGVHALGQVAHFDLTTNLDTYVVRNAIN
YHLIPHAIAILSVEKTDDKFHARFSAKKRHYLYKIINRYSPLTIDYNRAWLIHNPLNIENMIQAIEYIKGNHDFSSFRAR
HCQSKNPIKTIDDLKIIHNNQSINIHISAISFLHHQVRIIVGTLVECGKNNFSPEHIKNILKAKNRSYAGMTAPPYGLYF
VKVDY

Sequences:

>Translated_245_residues
MRYKITIEYNGSNFIGWQKQKHSSNSIQEILENAILKFSQQHTTVYVAGRTDAGVHALGQVAHFDLTTNLDTYVVRNAIN
YHLIPHAIAILSVEKTDDKFHARFSAKKRHYLYKIINRYSPLTIDYNRAWLIHNPLNIENMIQAIEYIKGNHDFSSFRAR
HCQSKNPIKTIDDLKIIHNNQSINIHISAISFLHHQVRIIVGTLVECGKNNFSPEHIKNILKAKNRSYAGMTAPPYGLYF
VKVDY
>Mature_245_residues
MRYKITIEYNGSNFIGWQKQKHSSNSIQEILENAILKFSQQHTTVYVAGRTDAGVHALGQVAHFDLTTNLDTYVVRNAIN
YHLIPHAIAILSVEKTDDKFHARFSAKKRHYLYKIINRYSPLTIDYNRAWLIHNPLNIENMIQAIEYIKGNHDFSSFRAR
HCQSKNPIKTIDDLKIIHNNQSINIHISAISFLHHQVRIIVGTLVECGKNNFSPEHIKNILKAKNRSYAGMTAPPYGLYF
VKVDY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=28.3088235294118, Blast_Score=108, Evalue=5e-24,
Organism=Escherichia coli, GI1788657, Length=246, Percent_Identity=41.0569105691057, Blast_Score=175, Evalue=2e-45,
Organism=Drosophila melanogaster, GI116007752, Length=303, Percent_Identity=28.3828382838284, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_EHRCR (Q2GGK1)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507432.1
- ProteinModelPortal:   Q2GGK1
- SMR:   Q2GGK1
- STRING:   Q2GGK1
- GeneID:   3927636
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0622
- TIGR:   ECH_0622
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   HSAIRRH
- PhylomeDB:   Q2GGK1
- ProtClustDB:   PRK00021
- BioCyc:   ECHA205920:ECH_0622-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28288; Mature: 28288

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKITIEYNGSNFIGWQKQKHSSNSIQEILENAILKFSQQHTTVYVAGRTDAGVHALGQ
CEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
VAHFDLTTNLDTYVVRNAINYHLIPHAIAILSVEKTDDKFHARFSAKKRHYLYKIINRYS
EEEEEEECCCHHHHHHHCCCEEEHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
PLTIDYNRAWLIHNPLNIENMIQAIEYIKGNHDFSSFRARHCQSKNPIKTIDDLKIIHNN
CEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECC
QSINIHISAISFLHHQVRIIVGTLVECGKNNFSPEHIKNILKAKNRSYAGMTAPPYGLYF
CEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEE
VKVDY
EEECC
>Mature Secondary Structure
MRYKITIEYNGSNFIGWQKQKHSSNSIQEILENAILKFSQQHTTVYVAGRTDAGVHALGQ
CEEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
VAHFDLTTNLDTYVVRNAINYHLIPHAIAILSVEKTDDKFHARFSAKKRHYLYKIINRYS
EEEEEEECCCHHHHHHHCCCEEEHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
PLTIDYNRAWLIHNPLNIENMIQAIEYIKGNHDFSSFRARHCQSKNPIKTIDDLKIIHNN
CEEEECCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCEEEEECC
QSINIHISAISFLHHQVRIIVGTLVECGKNNFSPEHIKNILKAKNRSYAGMTAPPYGLYF
CEEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEE
VKVDY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA