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The map label for this gene is pyrB

Identifier: 88658464

GI number: 88658464

Start: 628887

End: 629786

Strand: Direct

Name: pyrB

Synonym: ECH_0621

Alternate gene names: 88658464

Gene position: 628887-629786 (Clockwise)

Preceding gene: 88658324

Following gene: 88658037

Centisome position: 53.47

GC content: 29.56

Gene sequence:

>900_bases
ATGAAGACACTATTAGAGGTAAGTAATCTAACATCTGATGACATAGAATCTATTTTTAACCTTACAATACAGTACTTTAA
TAATACAAACTTAAACCATAATATCCTAACTGGAAAAGTCATTGTTAACCTATTTTTTGAAAGCTCAACACGTACTTTAT
CTTCTTTTGAAATTGCAGAAAAATCACTAGGTGCACATTCTATTACTTTAAACATTAACACATCATCTATAAATAAAGGA
GAAAGCATTATAGACACTATTTCAAATATAGATGCAATGAATCCTGATTTAATTATCATAAGGTCGCAATACAGTCAATT
TATCAAAAAAATATCAGAATATCTACCAAGCTGTTCTATAATAAATGCCGGAGATGGACATCATGAACACCCAACACAAG
CATTAACAGATTACTGTACTATACGTTACATAAAAAAAGATATTAATAATTTAAATATATCTATATGTGGAGATATATTA
CACAGTAGAGTCGCAAGATCAAATATAAGATTATTATCCAGATATGGAGCTAATATATCTTTAGTTGCTCCCCCAACATT
ATCATGTAACTTAACAGGTATATCACATATATATCACAACTTAATTGAAGGAATACGCAATGCAGATGTCATCATGTTGC
TAAGGCTACAAAAAGAACGCATTATTAATTGTGTTATTCCTTCAGAACAAGAATACTCTCACCTATATATGCTAAACCAT
GAAAAACTATTACATGCAAAAAAAGACGTCATAGTTATGCATCCAGGACCTACAAATAAAGGGATAGAAATCTCCAATAA
CGTGGCAGAAAAAAATTCTGTAATTCTATTACAAGTAAAAATGGGTGTTGCAGCACGTAAAGCTATATTACATTATTTAC
TGTACAATAAGTCAATATAA

Upstream 100 bases:

>100_bases
TTTATAAATAGAATTCAGATTGTAAAAAAATTTCATTCAATGTTTAAATAATATGTATAATCTTAATTAATAACACTTTT
ACATATAAAATAGGAGTATA

Downstream 100 bases:

>100_bases
CATCAATAATGCGATATAAAATTACTATAGAATATAATGGATCTAATTTTATTGGATGGCAAAAACAAAAACACTCATCA
AATTCCATTCAAGAAATTCT

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKTLLEVSNLTSDDIESIFNLTIQYFNNTNLNHNILTGKVIVNLFFESSTRTLSSFEIAEKSLGAHSITLNINTSSINKG
ESIIDTISNIDAMNPDLIIIRSQYSQFIKKISEYLPSCSIINAGDGHHEHPTQALTDYCTIRYIKKDINNLNISICGDIL
HSRVARSNIRLLSRYGANISLVAPPTLSCNLTGISHIYHNLIEGIRNADVIMLLRLQKERIINCVIPSEQEYSHLYMLNH
EKLLHAKKDVIVMHPGPTNKGIEISNNVAEKNSVILLQVKMGVAARKAILHYLLYNKSI

Sequences:

>Translated_299_residues
MKTLLEVSNLTSDDIESIFNLTIQYFNNTNLNHNILTGKVIVNLFFESSTRTLSSFEIAEKSLGAHSITLNINTSSINKG
ESIIDTISNIDAMNPDLIIIRSQYSQFIKKISEYLPSCSIINAGDGHHEHPTQALTDYCTIRYIKKDINNLNISICGDIL
HSRVARSNIRLLSRYGANISLVAPPTLSCNLTGISHIYHNLIEGIRNADVIMLLRLQKERIINCVIPSEQEYSHLYMLNH
EKLLHAKKDVIVMHPGPTNKGIEISNNVAEKNSVILLQVKMGVAARKAILHYLLYNKSI
>Mature_299_residues
MKTLLEVSNLTSDDIESIFNLTIQYFNNTNLNHNILTGKVIVNLFFESSTRTLSSFEIAEKSLGAHSITLNINTSSINKG
ESIIDTISNIDAMNPDLIIIRSQYSQFIKKISEYLPSCSIINAGDGHHEHPTQALTDYCTIRYIKKDINNLNISICGDIL
HSRVARSNIRLLSRYGANISLVAPPTLSCNLTGISHIYHNLIEGIRNADVIMLLRLQKERIINCVIPSEQEYSHLYMLNH
EKLLHAKKDVIVMHPGPTNKGIEISNNVAEKNSVILLQVKMGVAARKAILHYLLYNKSI

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=31.1475409836066, Blast_Score=142, Evalue=3e-34,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=30.42071197411, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI193204318, Length=326, Percent_Identity=33.4355828220859, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322331, Length=276, Percent_Identity=34.0579710144928, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24642586, Length=302, Percent_Identity=32.7814569536424, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_EHRCR (Q2GGK2)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507431.1
- ProteinModelPortal:   Q2GGK2
- SMR:   Q2GGK2
- STRING:   Q2GGK2
- GeneID:   3927102
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0621
- TIGR:   ECH_0621
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   ECHA205920:ECH_0621-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33550; Mature: 33550

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLEVSNLTSDDIESIFNLTIQYFNNTNLNHNILTGKVIVNLFFESSTRTLSSFEIAE
CCCHHHHHCCCHHHHHHHHHHEEEEECCCCCCCEEEEHEEEEEEEECCCCCHHHHHHHHH
KSLGAHSITLNINTSSINKGESIIDTISNIDAMNPDLIIIRSQYSQFIKKISEYLPSCSI
HCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEE
INAGDGHHEHPTQALTDYCTIRYIKKDINNLNISICGDILHSRVARSNIRLLSRYGANIS
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEE
LVAPPTLSCNLTGISHIYHNLIEGIRNADVIMLLRLQKERIINCVIPSEQEYSHLYMLNH
EEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECHHHEEEEECCCCCCCCEEEEECH
EKLLHAKKDVIVMHPGPTNKGIEISNNVAEKNSVILLQVKMGVAARKAILHYLLYNKSI
HHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTLLEVSNLTSDDIESIFNLTIQYFNNTNLNHNILTGKVIVNLFFESSTRTLSSFEIAE
CCCHHHHHCCCHHHHHHHHHHEEEEECCCCCCCEEEEHEEEEEEEECCCCCHHHHHHHHH
KSLGAHSITLNINTSSINKGESIIDTISNIDAMNPDLIIIRSQYSQFIKKISEYLPSCSI
HCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEE
INAGDGHHEHPTQALTDYCTIRYIKKDINNLNISICGDILHSRVARSNIRLLSRYGANIS
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEE
LVAPPTLSCNLTGISHIYHNLIEGIRNADVIMLLRLQKERIINCVIPSEQEYSHLYMLNH
EEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECHHHEEEEECCCCCCCCEEEEECH
EKLLHAKKDVIVMHPGPTNKGIEISNNVAEKNSVILLQVKMGVAARKAILHYLLYNKSI
HHHHHCCCCEEEECCCCCCCCEEECCCCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA