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The map label for this gene is metG [H]

Identifier: 88657921

GI number: 88657921

Start: 1024001

End: 1025521

Strand: Reverse

Name: metG [H]

Synonym: ECH_1000

Alternate gene names: 88657921

Gene position: 1025521-1024001 (Counterclockwise)

Preceding gene: 88657674

Following gene: 88658387

Centisome position: 87.19

GC content: 32.74

Gene sequence:

>1521_bases
ATGAATAATATATATATTACTACTCCTATTTATTATGTTAATGATGTGCCACATATAGGTCATGTTTATACAACTTTGAT
ATCTGATATTATCGCACGGTTTATGCGTCTTGATGGACATAGTGTAAAGTTTATAACTGGAACAGATGAACATGGGCAAA
AAATAGAAAAAGCAGCCCAAGAACGTAACATGTCATTATTAGATTTTACTGATAATACAAGTGCTGTTTTTAGACAGTTA
GCTGATGTTATGAACTATAGCTATGATGATTTTATTCGTACGACAGAAACAAGGCACAAAAAGACTGTGGTAGCTTTATG
GCAGCGTTTGTGTGATAATGGGGCAATATATTTAGGTAGTTATTCTGGTTGGTATTCGGTAAGAGATGAAACGTTTTATC
AGGAGAAAGAATTGGTCGATGGTAAAGCTCCTACAGGAGCTGATGTAGAATGGATAGAAGAGCCGAGTTATTTTTTTCGA
TTGTCAAATTTTCAGGAAAGGTTGTTAGCTTTTTACGAGGAAAATCCTGATTTTGTTATTCCTAAATATCGGTATAATGA
AGTTATATCATTTGTTAAATCAGGGTTGAAAGATCTTTCTGTATCAAGACAAAATGTTTTATGGGGAATAAAAGTACCAA
ATGATGATAAACATGTAATTTATGTTTGGGTAGATGCTTTAGCAAATTACTTGACAGTATTAGGGTTTCCTGATGAGAAC
CATAAAGATTATCAAGCTTATTGGGCAAGTGATAGTAGTTCTGTTTTACAGGTTGTGGGTAAGGATATATTGAGGTTTCA
TGCTGTATATTGGCCTGCTATTTTAATGGCAGCTGAATTGCCTTTACCTAAAAAAATCTTGGCACATGGTTGGTGGACTA
ATGAGGGGCAAAAAATTTCTAAGTCGTTAGGTAATGTTATTAAACCTTTTGACCTAGTAGAAGAATTTGGAGTTGATCAA
TTAAGATATTTTTTAATTAAAGAAATGCCAATAGGGAACGATGGAGATTTTAAAAGAAATAGTCTTATTAATTGTATAAA
TTATGATTTAGCAAATAATATAGGGAACCTTGTACAAAGAACTGTTTCACTATTGTATAAAGAATGTGGAGGGATAGTAC
CAACAGTAAGTGGCAATTTGCTGCAGGGTGAGGAAGTATTACCAGATTATCAAGAGATTCTTGAAAAGGTTAGAGATTGT
GTAATGCGTTGTAATCTGAATGAGATGATACATATTATAGAGCAATTATCTTCTGCAGCTAATGAATATATTGCATCACG
AGCGCCATGGAGGTTATCTAAGAGTGACCCTAAAATTATGGAAGCAGTATTGTATAAATTACTTGAATATATTAAATGTA
TAGGGTTGCTGTTGCAACCTGTCATGCCTAAATTATCATCTAAAATATTAGATCAAATTGGTTTACCAGAATGTAATCGT
GACTTTAGTCGATTTTCTATACCTATAAATATGAATACAGTTTTACCAAAACCAGAGCCTATTTTTGCAAAAATCTTATG
A

Upstream 100 bases:

>100_bases
TTATAAGTCAATATTTTGTAATATCTGCAAATATTAAGTTAATATAACTAAAATTATGTGATAATTTGCTAACTGTATAA
AGTAATAACAATGCTAACTT

Downstream 100 bases:

>100_bases
AACATATATGCTGAACTTATAAAAACTAAAGTGTAGATGATAAATACTATAGCAGTATCTTAATGTGAAGAGTGTTATTT
GTGTAAAGAAGTTTTTTGTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MNNIYITTPIYYVNDVPHIGHVYTTLISDIIARFMRLDGHSVKFITGTDEHGQKIEKAAQERNMSLLDFTDNTSAVFRQL
ADVMNYSYDDFIRTTETRHKKTVVALWQRLCDNGAIYLGSYSGWYSVRDETFYQEKELVDGKAPTGADVEWIEEPSYFFR
LSNFQERLLAFYEENPDFVIPKYRYNEVISFVKSGLKDLSVSRQNVLWGIKVPNDDKHVIYVWVDALANYLTVLGFPDEN
HKDYQAYWASDSSSVLQVVGKDILRFHAVYWPAILMAAELPLPKKILAHGWWTNEGQKISKSLGNVIKPFDLVEEFGVDQ
LRYFLIKEMPIGNDGDFKRNSLINCINYDLANNIGNLVQRTVSLLYKECGGIVPTVSGNLLQGEEVLPDYQEILEKVRDC
VMRCNLNEMIHIIEQLSSAANEYIASRAPWRLSKSDPKIMEAVLYKLLEYIKCIGLLLQPVMPKLSSKILDQIGLPECNR
DFSRFSIPINMNTVLPKPEPIFAKIL

Sequences:

>Translated_506_residues
MNNIYITTPIYYVNDVPHIGHVYTTLISDIIARFMRLDGHSVKFITGTDEHGQKIEKAAQERNMSLLDFTDNTSAVFRQL
ADVMNYSYDDFIRTTETRHKKTVVALWQRLCDNGAIYLGSYSGWYSVRDETFYQEKELVDGKAPTGADVEWIEEPSYFFR
LSNFQERLLAFYEENPDFVIPKYRYNEVISFVKSGLKDLSVSRQNVLWGIKVPNDDKHVIYVWVDALANYLTVLGFPDEN
HKDYQAYWASDSSSVLQVVGKDILRFHAVYWPAILMAAELPLPKKILAHGWWTNEGQKISKSLGNVIKPFDLVEEFGVDQ
LRYFLIKEMPIGNDGDFKRNSLINCINYDLANNIGNLVQRTVSLLYKECGGIVPTVSGNLLQGEEVLPDYQEILEKVRDC
VMRCNLNEMIHIIEQLSSAANEYIASRAPWRLSKSDPKIMEAVLYKLLEYIKCIGLLLQPVMPKLSSKILDQIGLPECNR
DFSRFSIPINMNTVLPKPEPIFAKIL
>Mature_506_residues
MNNIYITTPIYYVNDVPHIGHVYTTLISDIIARFMRLDGHSVKFITGTDEHGQKIEKAAQERNMSLLDFTDNTSAVFRQL
ADVMNYSYDDFIRTTETRHKKTVVALWQRLCDNGAIYLGSYSGWYSVRDETFYQEKELVDGKAPTGADVEWIEEPSYFFR
LSNFQERLLAFYEENPDFVIPKYRYNEVISFVKSGLKDLSVSRQNVLWGIKVPNDDKHVIYVWVDALANYLTVLGFPDEN
HKDYQAYWASDSSSVLQVVGKDILRFHAVYWPAILMAAELPLPKKILAHGWWTNEGQKISKSLGNVIKPFDLVEEFGVDQ
LRYFLIKEMPIGNDGDFKRNSLINCINYDLANNIGNLVQRTVSLLYKECGGIVPTVSGNLLQGEEVLPDYQEILEKVRDC
VMRCNLNEMIHIIEQLSSAANEYIASRAPWRLSKSDPKIMEAVLYKLLEYIKCIGLLLQPVMPKLSSKILDQIGLPECNR
DFSRFSIPINMNTVLPKPEPIFAKIL

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=514, Percent_Identity=35.6031128404669, Blast_Score=330, Evalue=2e-90,
Organism=Homo sapiens, GI14043022, Length=559, Percent_Identity=25.7602862254025, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1788432, Length=503, Percent_Identity=25.0497017892644, Blast_Score=175, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI212645096, Length=506, Percent_Identity=39.3280632411067, Blast_Score=323, Evalue=9e-89,
Organism=Caenorhabditis elegans, GI71992781, Length=398, Percent_Identity=39.4472361809045, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17540794, Length=559, Percent_Identity=25.2236135957066, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=522, Percent_Identity=32.9501915708812, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6321704, Length=547, Percent_Identity=24.3144424131627, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21358483, Length=500, Percent_Identity=36.2, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24655311, Length=553, Percent_Identity=26.4014466546112, Blast_Score=146, Evalue=4e-35,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 58123; Mature: 58123

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIYITTPIYYVNDVPHIGHVYTTLISDIIARFMRLDGHSVKFITGTDEHGQKIEKAAQ
CCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
ERNMSLLDFTDNTSAVFRQLADVMNYSYDDFIRTTETRHKKTVVALWQRLCDNGAIYLGS
HCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
YSGWYSVRDETFYQEKELVDGKAPTGADVEWIEEPSYFFRLSNFQERLLAFYEENPDFVI
CCCEEECCHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEE
PKYRYNEVISFVKSGLKDLSVSRQNVLWGIKVPNDDKHVIYVWVDALANYLTVLGFPDEN
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHEECCCCCC
HKDYQAYWASDSSSVLQVVGKDILRFHAVYWPAILMAAELPLPKKILAHGWWTNEGQKIS
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHH
KSLGNVIKPFDLVEEFGVDQLRYFLIKEMPIGNDGDFKRNSLINCINYDLANNIGNLVQR
HHHHHHCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TVSLLYKECGGIVPTVSGNLLQGEEVLPDYQEILEKVRDCVMRCNLNEMIHIIEQLSSAA
HHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NEYIASRAPWRLSKSDPKIMEAVLYKLLEYIKCIGLLLQPVMPKLSSKILDQIGLPECNR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DFSRFSIPINMNTVLPKPEPIFAKIL
CHHHEEEEEECCCCCCCCCCHHHCCC
>Mature Secondary Structure
MNNIYITTPIYYVNDVPHIGHVYTTLISDIIARFMRLDGHSVKFITGTDEHGQKIEKAAQ
CCCEEEECCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHH
ERNMSLLDFTDNTSAVFRQLADVMNYSYDDFIRTTETRHKKTVVALWQRLCDNGAIYLGS
HCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEC
YSGWYSVRDETFYQEKELVDGKAPTGADVEWIEEPSYFFRLSNFQERLLAFYEENPDFVI
CCCEEECCHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEEE
PKYRYNEVISFVKSGLKDLSVSRQNVLWGIKVPNDDKHVIYVWVDALANYLTVLGFPDEN
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEHHHHHHHHHEECCCCCC
HKDYQAYWASDSSSVLQVVGKDILRFHAVYWPAILMAAELPLPKKILAHGWWTNEGQKIS
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHH
KSLGNVIKPFDLVEEFGVDQLRYFLIKEMPIGNDGDFKRNSLINCINYDLANNIGNLVQR
HHHHHHCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
TVSLLYKECGGIVPTVSGNLLQGEEVLPDYQEILEKVRDCVMRCNLNEMIHIIEQLSSAA
HHHHHHHHHCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NEYIASRAPWRLSKSDPKIMEAVLYKLLEYIKCIGLLLQPVMPKLSSKILDQIGLPECNR
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
DFSRFSIPINMNTVLPKPEPIFAKIL
CHHHEEEEEECCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA