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The map label for this gene is rho

Identifier: 88658387

GI number: 88658387

Start: 1018570

End: 1019838

Strand: Reverse

Name: rho

Synonym: ECH_0994

Alternate gene names: 88658387

Gene position: 1019838-1018570 (Counterclockwise)

Preceding gene: 88657921

Following gene: 88658473

Centisome position: 86.7

GC content: 32.07

Gene sequence:

>1269_bases
GTGTTAGATCTGAGTGAGCTAAAAAAGAAGACAATAGAAGATTTGTTGAAAATAGCAGAAGACCTTGGAGTGGTTAGTAA
TGGTAGAATGCTGAAACAAGAAATAATATTTCACTTAATGAAAAAAGTAGTAAGTGATGGAGGTGCAGCAATAGGAGGAG
GGGTAGTAGAGATATTATCTGATGGTTTTGGATTCTTAAGGTCTCCTGAAGCAAATTATGCTGCAAGTGGTGATGATGTA
TATATTTCTGCAGGACAAATAAAAAAGTTTAATTTAAGAACAGGTGATATAGTAAGTGGTGAAATAAGAGCGCCAAGTGA
GAAAGAAAGGTATTTTACGTTAGTAAAAGCACATAGTATAAACTTTACAGATATGGCAAAGTTACAAAGATATGTACACT
TTGATGATTTAATACCTTTGTATCCAGAAGAAAGAATATTATTAGAATGTAATGATCCTATCAGCGTGAGTAAAAAAGAT
ATAAGCATGAGAGTAATAGATATAGTTGCTCCATTAGGTAAAGGACAAAGAGCGCTGATAGTTGCTCCACCAAGAGCAGG
AAAAACAATAATATTACAACAAATAGCACATTCAATATCTGTAAATCATCCAGATATAGAATTGATAGTTTTACTTATAG
GTGAAAGACCAGAAGAAGTGACAGATATGTGTAGATCTGTGAAAGGAGAGGTAGTAAGTTCTACATTTGATGAACCTGGA
TATAGACATGTACAACTTGCAGAAATTGTAATAGAAAAAGCAAAAAGAATGGTAGAACATAAAAAGAATGTAGTGATATT
ACTTGATTCAATTACAAGATTAGCAAGAGCATATAATTCAGTAATACCGTCATCAGGAAAAGTATTAACTGGTGGAGTAG
ATTCTAATGCTTTACAAAGACCAAAAAGATTTTTTGGAGCGGCAAGAAATATAGAGAATGGAGGATCATTGACAATAATA
GCAACGGCACTGATTGAAACAGGGTCAAAAATGGATGAAGTGATATTTGAAGAATTTAAAGGTACAGGAAATTGTGAAAT
AATATTAGATAGAAAAATATCTGATAAAAGAGTATATCCAGCAATAGATATTTCAAAATCTGGTACAAGGAAAGAAGATA
TGTTGATAGATAGTGTGTGTTTGAAGAAAGTATGGTTATTAAGAAGGCTATTGTCATCGATGGGATCTGTTGAAGCAATG
GAATTTTTAAGGGATAAATTGTTGATAACCAAAGACAATAATGAATTCTTTGATATGATGAATAGTTAA

Upstream 100 bases:

>100_bases
TGTATTAGAGAAAGAAGAAGAGAAAGAAGTAGTAGAGGTACAAGAGGAAATAATAGAAGAGAAAGAAGAAGAGAAAGTAC
CAGAACAACAAGAAGTAAAA

Downstream 100 bases:

>100_bases
TATGGGTATAGAAAATAAGAACTTTATAATGAATGTATAAGTGGTAGTACTTATAACTATAGAACTATATGTAGCTGTTT
AATACAAAAATGAATATTGT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho 1

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MLDLSELKKKTIEDLLKIAEDLGVVSNGRMLKQEIIFHLMKKVVSDGGAAIGGGVVEILSDGFGFLRSPEANYAASGDDV
YISAGQIKKFNLRTGDIVSGEIRAPSEKERYFTLVKAHSINFTDMAKLQRYVHFDDLIPLYPEERILLECNDPISVSKKD
ISMRVIDIVAPLGKGQRALIVAPPRAGKTIILQQIAHSISVNHPDIELIVLLIGERPEEVTDMCRSVKGEVVSSTFDEPG
YRHVQLAEIVIEKAKRMVEHKKNVVILLDSITRLARAYNSVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTII
ATALIETGSKMDEVIFEEFKGTGNCEIILDRKISDKRVYPAIDISKSGTRKEDMLIDSVCLKKVWLLRRLLSSMGSVEAM
EFLRDKLLITKDNNEFFDMMNS

Sequences:

>Translated_422_residues
MLDLSELKKKTIEDLLKIAEDLGVVSNGRMLKQEIIFHLMKKVVSDGGAAIGGGVVEILSDGFGFLRSPEANYAASGDDV
YISAGQIKKFNLRTGDIVSGEIRAPSEKERYFTLVKAHSINFTDMAKLQRYVHFDDLIPLYPEERILLECNDPISVSKKD
ISMRVIDIVAPLGKGQRALIVAPPRAGKTIILQQIAHSISVNHPDIELIVLLIGERPEEVTDMCRSVKGEVVSSTFDEPG
YRHVQLAEIVIEKAKRMVEHKKNVVILLDSITRLARAYNSVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTII
ATALIETGSKMDEVIFEEFKGTGNCEIILDRKISDKRVYPAIDISKSGTRKEDMLIDSVCLKKVWLLRRLLSSMGSVEAM
EFLRDKLLITKDNNEFFDMMNS
>Mature_422_residues
MLDLSELKKKTIEDLLKIAEDLGVVSNGRMLKQEIIFHLMKKVVSDGGAAIGGGVVEILSDGFGFLRSPEANYAASGDDV
YISAGQIKKFNLRTGDIVSGEIRAPSEKERYFTLVKAHSINFTDMAKLQRYVHFDDLIPLYPEERILLECNDPISVSKKD
ISMRVIDIVAPLGKGQRALIVAPPRAGKTIILQQIAHSISVNHPDIELIVLLIGERPEEVTDMCRSVKGEVVSSTFDEPG
YRHVQLAEIVIEKAKRMVEHKKNVVILLDSITRLARAYNSVIPSSGKVLTGGVDSNALQRPKRFFGAARNIENGGSLTII
ATALIETGSKMDEVIFEEFKGTGNCEIILDRKISDKRVYPAIDISKSGTRKEDMLIDSVCLKKVWLLRRLLSSMGSVEAM
EFLRDKLLITKDNNEFFDMMNS

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family

Homologues:

Organism=Escherichia coli, GI1790217, Length=419, Percent_Identity=59.6658711217184, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1788251, Length=244, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): RHO1_EHRCR (P0CH92)

Other databases:

- EMBL:   CP000236
- RefSeq:   YP_507024.1
- RefSeq:   YP_507781.1
- SMR:   P0CH92
- GeneID:   3927381
- GeneID:   3927646
- GenomeReviews:   CP000236_GR
- KEGG:   ech:ECH_0994
- TIGR:   ECH_0994
- eggNOG:   COG1158
- HOGENOM:   HBG335262
- OMA:   YNYLPGP
- BioCyc:   ECHA205920:ECH_0200-MONOMER
- BioCyc:   ECHA205920:ECH_0994-MONOMER
- HAMAP:   MF_01884
- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00382
- SMART:   SM00357
- SMART:   SM00959
- TIGRFAMs:   TIGR00767

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind; SSF50249 Nucleic_acid_OB; SSF68912 Rho_N

EC number: NA

Molecular weight: Translated: 46865; Mature: 46865

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLSELKKKTIEDLLKIAEDLGVVSNGRMLKQEIIFHLMKKVVSDGGAAIGGGVVEILS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DGFGFLRSPEANYAASGDDVYISAGQIKKFNLRTGDIVSGEIRAPSEKERYFTLVKAHSI
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEECCCCCCCCHHHEEEEEEECCC
NFTDMAKLQRYVHFDDLIPLYPEERILLECNDPISVSKKDISMRVIDIVAPLGKGQRALI
CHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHEEEEEHHHCCCCCCEEEE
VAPPRAGKTIILQQIAHSISVNHPDIELIVLLIGERPEEVTDMCRSVKGEVVSSTFDEPG
EECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCC
YRHVQLAEIVIEKAKRMVEHKKNVVILLDSITRLARAYNSVIPSSGKVLTGGVDSNALQR
CCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHH
PKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKISDKRVYP
HHHHHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
AIDISKSGTRKEDMLIDSVCLKKVWLLRRLLSSMGSVEAMEFLRDKLLITKDNNEFFDMM
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCHHHHHC
NS
CC
>Mature Secondary Structure
MLDLSELKKKTIEDLLKIAEDLGVVSNGRMLKQEIIFHLMKKVVSDGGAAIGGGVVEILS
CCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
DGFGFLRSPEANYAASGDDVYISAGQIKKFNLRTGDIVSGEIRAPSEKERYFTLVKAHSI
CCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCEEECCCCCCCCHHHEEEEEEECCC
NFTDMAKLQRYVHFDDLIPLYPEERILLECNDPISVSKKDISMRVIDIVAPLGKGQRALI
CHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHEEEEEHHHCCCCCCEEEE
VAPPRAGKTIILQQIAHSISVNHPDIELIVLLIGERPEEVTDMCRSVKGEVVSSTFDEPG
EECCCCCCHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHCCCCCC
YRHVQLAEIVIEKAKRMVEHKKNVVILLDSITRLARAYNSVIPSSGKVLTGGVDSNALQR
CCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHH
PKRFFGAARNIENGGSLTIIATALIETGSKMDEVIFEEFKGTGNCEIILDRKISDKRVYP
HHHHHHHHCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
AIDISKSGTRKEDMLIDSVCLKKVWLLRRLLSSMGSVEAMEFLRDKLLITKDNNEFFDMM
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCEEEECCCCHHHHHC
NS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA