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The map label for this gene is lipB

Identifier: 88607528

GI number: 88607528

Start: 241409

End: 242035

Strand: Direct

Name: lipB

Synonym: APH_0243

Alternate gene names: 88607528

Gene position: 241409-242035 (Clockwise)

Preceding gene: 88606961

Following gene: 88607036

Centisome position: 16.41

GC content: 43.06

Gene sequence:

>627_bases
GTGGATATCGAGTGGCAAATTTCCAGTGGGTTAGTGGAGTACGAAGTTGCAGTTAATGCAATGGTTTCAAGAGTAGATAA
AATAGTACAGGGTTCTGAGAGGGAAATGGTATGGTTGCTAGAGCATCCGCCTGTATACACTGCTGGTACGAGTGCCAATT
CTAGTGATCTTCTGATAGATAATCTTTTTCCGGTTATAAAAACCACTCGTGGCGGAAAATACTCCTACCACGGTCCAGGG
CAGAGGGTAGTATACGTTATGCTTGACCTCAAGCGCCGGAATAGGTGCGATATTCGTGCATATGTGAGAGACTTAGGTGT
ATGGATAGTTAATACTCTCGCGGAGTTTGCTATTGATAGCTATTTCAGTAGCGAAAATATTGGTGTATGGGTACAAAATG
ACATGCATAGCGAAAAAATTGCTGCGTTTGGTATAAGGTTACGTCGGTGGGTGACTTACCATGGTGTTGCCATAAACATA
AGTACAGACCTTACACATTACTCCGGAATTGTTCCCTGCGGCATTTTGGGTTCAGGGGTTACATCTTTGAGGGCCTTGGG
CAAAGAAGTGACGTTTGAACAATTTGACTCGGCTCTCAAAAAAGAGTTCTACAAAGTTTTTGCATAA

Upstream 100 bases:

>100_bases
TTAAGACTCATGGGTTGTTATAAGCTATAATGGAGGACTTGACGGGGAATATCATTCAAAGTAGCATGCGTGATTCCTAC
ATTATATATGCAGATTATTC

Downstream 100 bases:

>100_bases
AAGCATACGGACATCAACTCTACCTGCTTTGTATACTCTTGTGTTGATTAGGACGCCCGAGAGATAGGGGGTTTGATGCT
CTGTTCAGGGTAATAGGGCC

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MDIEWQISSGLVEYEVAVNAMVSRVDKIVQGSEREMVWLLEHPPVYTAGTSANSSDLLIDNLFPVIKTTRGGKYSYHGPG
QRVVYVMLDLKRRNRCDIRAYVRDLGVWIVNTLAEFAIDSYFSSENIGVWVQNDMHSEKIAAFGIRLRRWVTYHGVAINI
STDLTHYSGIVPCGILGSGVTSLRALGKEVTFEQFDSALKKEFYKVFA

Sequences:

>Translated_208_residues
MDIEWQISSGLVEYEVAVNAMVSRVDKIVQGSEREMVWLLEHPPVYTAGTSANSSDLLIDNLFPVIKTTRGGKYSYHGPG
QRVVYVMLDLKRRNRCDIRAYVRDLGVWIVNTLAEFAIDSYFSSENIGVWVQNDMHSEKIAAFGIRLRRWVTYHGVAINI
STDLTHYSGIVPCGILGSGVTSLRALGKEVTFEQFDSALKKEFYKVFA
>Mature_208_residues
MDIEWQISSGLVEYEVAVNAMVSRVDKIVQGSEREMVWLLEHPPVYTAGTSANSSDLLIDNLFPVIKTTRGGKYSYHGPG
QRVVYVMLDLKRRNRCDIRAYVRDLGVWIVNTLAEFAIDSYFSSENIGVWVQNDMHSEKIAAFGIRLRRWVTYHGVAINI
STDLTHYSGIVPCGILGSGVTSLRALGKEVTFEQFDSALKKEFYKVFA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=187, Percent_Identity=35.8288770053476, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI87081767, Length=182, Percent_Identity=37.9120879120879, Blast_Score=129, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323268, Length=214, Percent_Identity=28.5046728971963, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355483, Length=180, Percent_Identity=38.8888888888889, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_ANAPZ (Q2GL91)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504860.1
- ProteinModelPortal:   Q2GL91
- SMR:   Q2GL91
- STRING:   Q2GL91
- GeneID:   3930923
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0243
- NMPDR:   fig|212042.5.peg.232
- TIGR:   APH_0243
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q2GL91
- ProtClustDB:   CLSK2463746
- BioCyc:   APHA212042:APH_0243-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 23461; Mature: 23461

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEWQISSGLVEYEVAVNAMVSRVDKIVQGSEREMVWLLEHPPVYTAGTSANSSDLLID
CCEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCEEHH
NLFPVIKTTRGGKYSYHGPGQRVVYVMLDLKRRNRCDIRAYVRDLGVWIVNTLAEFAIDS
HHHHHHEECCCCCEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YFSSENIGVWVQNDMHSEKIAAFGIRLRRWVTYHGVAINISTDLTHYSGIVPCGILGSGV
HCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEECCEEEEEECCCHHHCCCCCHHHHHCCH
TSLRALGKEVTFEQFDSALKKEFYKVFA
HHHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIEWQISSGLVEYEVAVNAMVSRVDKIVQGSEREMVWLLEHPPVYTAGTSANSSDLLID
CCEEEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCCCCCEEHH
NLFPVIKTTRGGKYSYHGPGQRVVYVMLDLKRRNRCDIRAYVRDLGVWIVNTLAEFAIDS
HHHHHHEECCCCCEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YFSSENIGVWVQNDMHSEKIAAFGIRLRRWVTYHGVAINISTDLTHYSGIVPCGILGSGV
HCCCCCCEEEEECCCCHHHHHHHHHHHHHEEEECCEEEEEECCCHHHCCCCCHHHHHCCH
TSLRALGKEVTFEQFDSALKKEFYKVFA
HHHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA