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The map label for this gene is ruvA

Identifier: 88607371

GI number: 88607371

Start: 169853

End: 170428

Strand: Reverse

Name: ruvA

Synonym: APH_0167

Alternate gene names: 88607371

Gene position: 170428-169853 (Counterclockwise)

Preceding gene: 88606928

Following gene: 88607797

Centisome position: 11.58

GC content: 42.19

Gene sequence:

>576_bases
ATGATCGGAACTCTCTCAGGTATTATAGAAGAAGTACAGGATACTAGCATCATACTCGCGGTGGGTGGCGTCGGCTACAT
AGTACATGTCTCCTATAGAACGCTAATCAATTGTAAGCGTGGCGACTCCATAAAACTGTATATAGAAACATATGTGAATA
GAGACAATGTGCCTCAGCTTTATGGATTTACTGATACTGAAGAGCAAAATTGCTTGAAGATGCTAATAAAGGTCAGCGGA
ATAAACTACAGAACCGCTTTGGCTATACTGGATAGGTTAACGCCCGATCAGCTATTCTCGGCCATTGTGAATGAAGATAA
AGCTGCGCTGAAAGTAGGAGGCGTAGGCGCTAAGTTAATTAATAGGATTTTCACGGAATTGACTCCTCTGGTACAGAAAT
TAGAGTTCAATATAATGGACAAAAGAGGACCATCTGTAGAAGATAGCGATGCATTGTCTGCTCTACTCAGTTTGGGATAC
GAAAAAACTCGGGTACTCAACGCTTTAGAGAAGGTAGGAGTCTCCCACAACCTGTCTGACACAGTTCGTTTTGCCCTGAA
AGAGTTATCGAAGTGA

Upstream 100 bases:

>100_bases
TATCGCTGCTGTTTGTCATTCTGACGTCGCTGCGCTTTTATACGCTGTTGAATAATAGGCAGACGTCAAGTAGTCTATAC
CCTTTTTACCATGAAACAAC

Downstream 100 bases:

>100_bases
ATGAGCTTTTACATCAACACAAAGCAATACCTGAAGATGAACGTAATTTTGCATTACGTCCGAGCTTGATCGAGGAATTT
GTCGGGCAGAGTGAGATAAT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MIGTLSGIIEEVQDTSIILAVGGVGYIVHVSYRTLINCKRGDSIKLYIETYVNRDNVPQLYGFTDTEEQNCLKMLIKVSG
INYRTALAILDRLTPDQLFSAIVNEDKAALKVGGVGAKLINRIFTELTPLVQKLEFNIMDKRGPSVEDSDALSALLSLGY
EKTRVLNALEKVGVSHNLSDTVRFALKELSK

Sequences:

>Translated_191_residues
MIGTLSGIIEEVQDTSIILAVGGVGYIVHVSYRTLINCKRGDSIKLYIETYVNRDNVPQLYGFTDTEEQNCLKMLIKVSG
INYRTALAILDRLTPDQLFSAIVNEDKAALKVGGVGAKLINRIFTELTPLVQKLEFNIMDKRGPSVEDSDALSALLSLGY
EKTRVLNALEKVGVSHNLSDTVRFALKELSK
>Mature_191_residues
MIGTLSGIIEEVQDTSIILAVGGVGYIVHVSYRTLINCKRGDSIKLYIETYVNRDNVPQLYGFTDTEEQNCLKMLIKVSG
INYRTALAILDRLTPDQLFSAIVNEDKAALKVGGVGAKLINRIFTELTPLVQKLEFNIMDKRGPSVEDSDALSALLSLGY
EKTRVLNALEKVGVSHNLSDTVRFALKELSK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=200, Percent_Identity=30, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_ANAPZ (Q2GLG0)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504791.1
- ProteinModelPortal:   Q2GLG0
- SMR:   Q2GLG0
- STRING:   Q2GLG0
- GeneID:   3930259
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0167
- NMPDR:   fig|212042.5.peg.162
- TIGR:   APH_0167
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   RIISELT
- ProtClustDB:   PRK14600
- BioCyc:   APHA212042:APH_0167-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =3.6.4.12

Molecular weight: Translated: 21079; Mature: 21079

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTLSGIIEEVQDTSIILAVGGVGYIVHVSYRTLINCKRGDSIKLYIETYVNRDNVPQL
CCCHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHHEECCCCCEEEEEEEEECCCCCCCCC
YGFTDTEEQNCLKMLIKVSGINYRTALAILDRLTPDQLFSAIVNEDKAALKVGGVGAKLI
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHEEECCCCHHHH
NRIFTELTPLVQKLEFNIMDKRGPSVEDSDALSALLSLGYEKTRVLNALEKVGVSHNLSD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHH
TVRFALKELSK
HHHHHHHHHCC
>Mature Secondary Structure
MIGTLSGIIEEVQDTSIILAVGGVGYIVHVSYRTLINCKRGDSIKLYIETYVNRDNVPQL
CCCHHHHHHHHHCCCEEEEEECCCEEEEEEHHHHHEECCCCCEEEEEEEEECCCCCCCCC
YGFTDTEEQNCLKMLIKVSGINYRTALAILDRLTPDQLFSAIVNEDKAALKVGGVGAKLI
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCHHHEEECCCCHHHH
NRIFTELTPLVQKLEFNIMDKRGPSVEDSDALSALLSLGYEKTRVLNALEKVGVSHNLSD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHH
TVRFALKELSK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA