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The map label for this gene is ruvB

Identifier: 88607797

GI number: 88607797

Start: 168867

End: 169856

Strand: Reverse

Name: ruvB

Synonym: APH_0166

Alternate gene names: 88607797

Gene position: 169856-168867 (Counterclockwise)

Preceding gene: 88607371

Following gene: 88607203

Centisome position: 11.54

GC content: 42.73

Gene sequence:

>990_bases
GTGAATGAGCTTTTACATCAACACAAAGCAATACCTGAAGATGAACGTAATTTTGCATTACGTCCGAGCTTGATCGAGGA
ATTTGTCGGGCAGAGTGAGATAATAGAGAATCTCAAGGTATTCATCAAGTCAGCCTATGAAAGAAGAGCAACACTGGACC
ATGTGCTGTTATATGGGCCCCCAGGGCTGGGCAAAACTACACTAGCGCATATAATCGCCAAGGAACTTAAAGTGAGCTTG
AGGTCAACTTCCGGACCTCTTTTGAGTAAAGCTGGAGATCTTGCCGCTATACTCACGAACCTACAGCCCATGGATGTGCT
ATTTATAGACGAGATACATAGACTACACAGAAACATCGAAGAAATCTTATACTCCGCCATGGAAGACTGCTGTCTTGATA
TTGTTGTCGGTGAAGGCTGCGGTGCAAGAACGCTGCGCATTGATCTCCCCGCTTTTACTTTAGTAGGCGCAACGACAAGA
TTTGGCTTAATATCCAACCCTCTTAGAGATAGGTTCGGTATTCCGTTGCATCTAGAATTCTACTCTGTAGAAGAGTTAAT
GCTGGTGATAAAGCGAGCCGCACATGTGATTTGTACCGACATTGATGATAGCGGCGCTTATGAGATTGCCTCACGTTCTA
GAGGTACTCCGAGAATTGCACTTCGATTATTTAGGAGAGTGCGTGATTTCATGGTTGTGGAAAGACAGAGTATAATAGAC
AATCATTTTGCTGATTCTGCACTTTTCAACCTTGGTGTAGATAAATCTGGTCTCGATAAGATGGATATCAAGTATCTCAG
CTTCATCTATGAGGCAAAAAACGCAGTCGGAATAGAGACTATTGCTGCTGCCCTTTCAGAAGATGTAGGTAATATTGAAG
AGACCATTGAACCGTATCTACTGAAAATAGGGTTCATACAACGCACACCTCGTGGAAGAATTCTTACCACAAAGGCCATT
GAGCACTTAATGAACTATAAATATATCTAG

Upstream 100 bases:

>100_bases
TGGGATACGAAAAAACTCGGGTACTCAACGCTTTAGAGAAGGTAGGAGTCTCCCACAACCTGTCTGACACAGTTCGTTTT
GCCCTGAAAGAGTTATCGAA

Downstream 100 bases:

>100_bases
GTTGTTTTTGCATGATGATCCGCAGCAAACTGCTGCTTCTCATTTTCACCAAAATATGGATGTACCTTCTTCAATATCAT
AGTTCAACGCATCATCTGGG

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNELLHQHKAIPEDERNFALRPSLIEEFVGQSEIIENLKVFIKSAYERRATLDHVLLYGPPGLGKTTLAHIIAKELKVSL
RSTSGPLLSKAGDLAAILTNLQPMDVLFIDEIHRLHRNIEEILYSAMEDCCLDIVVGEGCGARTLRIDLPAFTLVGATTR
FGLISNPLRDRFGIPLHLEFYSVEELMLVIKRAAHVICTDIDDSGAYEIASRSRGTPRIALRLFRRVRDFMVVERQSIID
NHFADSALFNLGVDKSGLDKMDIKYLSFIYEAKNAVGIETIAAALSEDVGNIEETIEPYLLKIGFIQRTPRGRILTTKAI
EHLMNYKYI

Sequences:

>Translated_329_residues
MNELLHQHKAIPEDERNFALRPSLIEEFVGQSEIIENLKVFIKSAYERRATLDHVLLYGPPGLGKTTLAHIIAKELKVSL
RSTSGPLLSKAGDLAAILTNLQPMDVLFIDEIHRLHRNIEEILYSAMEDCCLDIVVGEGCGARTLRIDLPAFTLVGATTR
FGLISNPLRDRFGIPLHLEFYSVEELMLVIKRAAHVICTDIDDSGAYEIASRSRGTPRIALRLFRRVRDFMVVERQSIID
NHFADSALFNLGVDKSGLDKMDIKYLSFIYEAKNAVGIETIAAALSEDVGNIEETIEPYLLKIGFIQRTPRGRILTTKAI
EHLMNYKYI
>Mature_329_residues
MNELLHQHKAIPEDERNFALRPSLIEEFVGQSEIIENLKVFIKSAYERRATLDHVLLYGPPGLGKTTLAHIIAKELKVSL
RSTSGPLLSKAGDLAAILTNLQPMDVLFIDEIHRLHRNIEEILYSAMEDCCLDIVVGEGCGARTLRIDLPAFTLVGATTR
FGLISNPLRDRFGIPLHLEFYSVEELMLVIKRAAHVICTDIDDSGAYEIASRSRGTPRIALRLFRRVRDFMVVERQSIID
NHFADSALFNLGVDKSGLDKMDIKYLSFIYEAKNAVGIETIAAALSEDVGNIEETIEPYLLKIGFIQRTPRGRILTTKAI
EHLMNYKYI

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=324, Percent_Identity=53.0864197530864, Blast_Score=348, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_ANAPZ (Q2GLG1)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504790.1
- ProteinModelPortal:   Q2GLG1
- SMR:   Q2GLG1
- STRING:   Q2GLG1
- GeneID:   3930839
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0166
- NMPDR:   fig|212042.5.peg.161
- TIGR:   APH_0166
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   APHA212042:APH_0166-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37036; Mature: 37036

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELLHQHKAIPEDERNFALRPSLIEEFVGQSEIIENLKVFIKSAYERRATLDHVLLYGP
CCHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECC
PGLGKTTLAHIIAKELKVSLRSTSGPLLSKAGDLAAILTNLQPMDVLFIDEIHRLHRNIE
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EILYSAMEDCCLDIVVGEGCGARTLRIDLPAFTLVGATTRFGLISNPLRDRFGIPLHLEF
HHHHHHHHHHHHEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHCCCCEEEEH
YSVEELMLVIKRAAHVICTDIDDSGAYEIASRSRGTPRIALRLFRRVRDFMVVERQSIID
HHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NHFADSALFNLGVDKSGLDKMDIKYLSFIYEAKNAVGIETIAAALSEDVGNIEETIEPYL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
LKIGFIQRTPRGRILTTKAIEHLMNYKYI
HHHHHHEECCCCCEEEHHHHHHHHCCCCC
>Mature Secondary Structure
MNELLHQHKAIPEDERNFALRPSLIEEFVGQSEIIENLKVFIKSAYERRATLDHVLLYGP
CCHHHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCEEEEECC
PGLGKTTLAHIIAKELKVSLRSTSGPLLSKAGDLAAILTNLQPMDVLFIDEIHRLHRNIE
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EILYSAMEDCCLDIVVGEGCGARTLRIDLPAFTLVGATTRFGLISNPLRDRFGIPLHLEF
HHHHHHHHHHHHEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHCCCCEEEEH
YSVEELMLVIKRAAHVICTDIDDSGAYEIASRSRGTPRIALRLFRRVRDFMVVERQSIID
HHHHHHHHHHHHHHHEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NHFADSALFNLGVDKSGLDKMDIKYLSFIYEAKNAVGIETIAAALSEDVGNIEETIEPYL
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHH
LKIGFIQRTPRGRILTTKAIEHLMNYKYI
HHHHHHEECCCCCEEEHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA