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The map label for this gene is petB [H]

Identifier: 88607218

GI number: 88607218

Start: 414288

End: 415514

Strand: Direct

Name: petB [H]

Synonym: APH_0402

Alternate gene names: 88607218

Gene position: 414288-415514 (Clockwise)

Preceding gene: 88607923

Following gene: 88607854

Centisome position: 28.16

GC content: 40.42

Gene sequence:

>1227_bases
ATGCAAAAGAACGAGAATAACGCTGCTGAGTGCGGCAAGGGTGTATTAGCCTGGATAGAGTACAGACTTCCTGTATGTGC
GTTTTTCAGGGAATTGGCGAATTATCAGGTGCCCAAGAATTTGAACTATGCTTGGAATTTTGGGTCATTGGCTGGTATTG
CCTTAATAATCCAGGTGATTACGGGTATATTTTTAGCTATGCACTATACTCCGCATGTTGATCATGCCTTTAATAGTGTG
GAGCGCATAATGCGAGATGTGCATTATGGTTGGCTCGTTAGGTATACGCATGCTGTTGGGGCATCCTTTTTTTTTATTGT
TGTTTATATACACATTTTCAGAGGGCTCTATTATGGGTCATATAAGAAACCTAGGGAGCTGGTATGGTTTGCGGGGATAA
TTATCTTTTTGGTTATGATGGCTACTGCATTCATGGGGTACGTTCTACCATGGGGGCAGATGAGTTTTTGGGGAGCTACT
GTAATTACAAATCTTTTCTCAGCTGTGCCTGTGGTAGGTGAGGGGATTGTTAGATGGTTATGGGGTGGATTCTCCGTTGA
TAATCCTACGCTGAACAGGTTTTTTGCGCTGCATTATTTATTGCCGTTTACGCTTATAGGTTTGGCGGGGCTGCATGTTA
TAGCTCTACACAGATTCGGTTCTGGAAATCCTAGCGGTGTTGAGGTGAAATCTAAGCGTGATACCATTCCTATATGGCCG
TATTTTATAATCAAGGATTGTATAACTTTTGGGTTATTTTTTGTATTTTTGTACTTATTTGTATTTTATGCGCCAAATTA
TTTAGGGCATCCGGATAATTATATTGAGGCAGATCCTATGGTTACTCCGGAGCATATAGTGCCAGAATGGTACTTCTTGC
CGTTTTACGCAATGCTTCGCTCAATTCCTGATAAGTTGTTGGGTGTTGCAACGATGTTTGGTTCGATATTGGTGTGGTTT
TTGCTGCCATTTTTAGATAGGTCTGAAGTCAAAAGTGGTAAATATCGCCCTGTCTTTAAAGTTTTCTATTGGATTTTTGT
GCTTAATTTTTGTCTTTTGATGTGGATAGGTGGTCAGGAGGTAAAGGAGCCTTTTATTTCTCTGGGTAGGCTTTCGACAC
TTTATTACTTTTCGTATTTTTCTATAGTTTTGCCTTTGCTATCGAAATATGAAAAATGCAAAGAATTGCCTAGTACCCTA
AGTGACACTGTGCCGGAGATGAAGTGA

Upstream 100 bases:

>100_bases
CTGGGCCTGCGCCTTTAAATTTGCCAATACCTGATTATCATTTTGTAGATGATAGTACTATGGTAATTGGCAAGAAGGGT
ACTGCTGTGTAGGGGTAGAG

Downstream 100 bases:

>100_bases
TATGATGCGTGCGTCGACTTTTTTATGGATATTCGTGTGTTTGCTGCCTTTAGGTGCAGTATGTAGGCATGTTGTGCCTG
GAGGTGATGTACCGAAATGG

Product: ubiquinol-cytochrome c reductase, cytochrome b

Products: Q; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQKNENNAAECGKGVLAWIEYRLPVCAFFRELANYQVPKNLNYAWNFGSLAGIALIIQVITGIFLAMHYTPHVDHAFNSV
ERIMRDVHYGWLVRYTHAVGASFFFIVVYIHIFRGLYYGSYKKPRELVWFAGIIIFLVMMATAFMGYVLPWGQMSFWGAT
VITNLFSAVPVVGEGIVRWLWGGFSVDNPTLNRFFALHYLLPFTLIGLAGLHVIALHRFGSGNPSGVEVKSKRDTIPIWP
YFIIKDCITFGLFFVFLYLFVFYAPNYLGHPDNYIEADPMVTPEHIVPEWYFLPFYAMLRSIPDKLLGVATMFGSILVWF
LLPFLDRSEVKSGKYRPVFKVFYWIFVLNFCLLMWIGGQEVKEPFISLGRLSTLYYFSYFSIVLPLLSKYEKCKELPSTL
SDTVPEMK

Sequences:

>Translated_408_residues
MQKNENNAAECGKGVLAWIEYRLPVCAFFRELANYQVPKNLNYAWNFGSLAGIALIIQVITGIFLAMHYTPHVDHAFNSV
ERIMRDVHYGWLVRYTHAVGASFFFIVVYIHIFRGLYYGSYKKPRELVWFAGIIIFLVMMATAFMGYVLPWGQMSFWGAT
VITNLFSAVPVVGEGIVRWLWGGFSVDNPTLNRFFALHYLLPFTLIGLAGLHVIALHRFGSGNPSGVEVKSKRDTIPIWP
YFIIKDCITFGLFFVFLYLFVFYAPNYLGHPDNYIEADPMVTPEHIVPEWYFLPFYAMLRSIPDKLLGVATMFGSILVWF
LLPFLDRSEVKSGKYRPVFKVFYWIFVLNFCLLMWIGGQEVKEPFISLGRLSTLYYFSYFSIVLPLLSKYEKCKELPSTL
SDTVPEMK
>Mature_408_residues
MQKNENNAAECGKGVLAWIEYRLPVCAFFRELANYQVPKNLNYAWNFGSLAGIALIIQVITGIFLAMHYTPHVDHAFNSV
ERIMRDVHYGWLVRYTHAVGASFFFIVVYIHIFRGLYYGSYKKPRELVWFAGIIIFLVMMATAFMGYVLPWGQMSFWGAT
VITNLFSAVPVVGEGIVRWLWGGFSVDNPTLNRFFALHYLLPFTLIGLAGLHVIALHRFGSGNPSGVEVKSKRDTIPIWP
YFIIKDCITFGLFFVFLYLFVFYAPNYLGHPDNYIEADPMVTPEHIVPEWYFLPFYAMLRSIPDKLLGVATMFGSILVWF
LLPFLDRSEVKSGKYRPVFKVFYWIFVLNFCLLMWIGGQEVKEPFISLGRLSTLYYFSYFSIVLPLLSKYEKCKELPSTL
SDTVPEMK

Specific function: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis [H]

COG id: COG1290

COG function: function code C; Cytochrome b subunit of the bc complex

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome b family [H]

Homologues:

Organism=Homo sapiens, GI251831119, Length=356, Percent_Identity=50.561797752809, Blast_Score=357, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6226529, Length=355, Percent_Identity=57.4647887323944, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6226532, Length=233, Percent_Identity=58.3690987124464, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6226531, Length=152, Percent_Identity=60.5263157894737, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6226530, Length=136, Percent_Identity=52.9411764705882, Blast_Score=149, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016175
- InterPro:   IPR005798
- InterPro:   IPR005797
- InterPro:   IPR016174 [H]

Pfam domain/function: PF00032 Cytochrom_B_C; PF00033 Cytochrom_B_N [H]

EC number: 1.10.2.2

Molecular weight: Translated: 47079; Mature: 47079

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS51002 CYTB_NTER ; PS51003 CYTB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQKNENNAAECGKGVLAWIEYRLPVCAFFRELANYQVPKNLNYAWNFGSLAGIALIIQVI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
TGIFLAMHYTPHVDHAFNSVERIMRDVHYGWLVRYTHAVGASFFFIVVYIHIFRGLYYGS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YKKPRELVWFAGIIIFLVMMATAFMGYVLPWGQMSFWGATVITNLFSAVPVVGEGIVRWL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGGFSVDNPTLNRFFALHYLLPFTLIGLAGLHVIALHRFGSGNPSGVEVKSKRDTIPIWP
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCH
YFIIKDCITFGLFFVFLYLFVFYAPNYLGHPDNYIEADPMVTPEHIVPEWYFLPFYAMLR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHH
SIPDKLLGVATMFGSILVWFLLPFLDRSEVKSGKYRPVFKVFYWIFVLNFCLLMWIGGQE
HHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
VKEPFISLGRLSTLYYFSYFSIVLPLLSKYEKCKELPSTLSDTVPEMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: QH2; ferricytochrome c

Specific reaction: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]