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The map label for this gene is gph [C]

Identifier: 88607019

GI number: 88607019

Start: 187549

End: 188172

Strand: Reverse

Name: gph [C]

Synonym: APH_0187

Alternate gene names: 88607019

Gene position: 188172-187549 (Counterclockwise)

Preceding gene: 88606895

Following gene: 88607114

Centisome position: 12.79

GC content: 39.26

Gene sequence:

>624_bases
ATGCAGCCTCCTGTGGCAGTGATGTTTGATTGGTGTAATACCATCGTGAAAACCGACAAACTAGATTTTGCCATATTATC
TAGGGTACTCACATCTATGAACCTTCCACACATAGATCTCTCTGATATTCCAAGCAACAAAGTTGAGCTCTATCTTTCAT
GCGCTCTAGGGTACCGCTGGAAAGAAGCAGTGAATATGTACGAGAAAATTGCGGGTATGAATATCAATGCGCGCTCTATG
TCTCCAAATGAAAATGTACTAGAGCTTTTAGAGTTTCTTTCCCATCACAAAATCAATATGGGTATTGTAAGCAATAAACG
CGGACCTAATTTGCGTAATGAAGTCCAAAACTTAGGGCTAGGAAAATATTTCTCAGTAATATTAGGATCAGGAGATACAG
CTGAAAACAAACCATCACCAGAACCAATATTCGCTGCATTAGACATCATGGGCATTGCACCTAGTGAGCGAGTATTTTTC
GTGGGAGATAGCGATTCCGATATTGAAAGCGCAAAAAGAGCTAGATGTGTTCCAATAGCATACGATAACACCTCAATAGA
AGGAGTATTATCCTTTGCTAACTTTAAAGACCTTGGTAACTTTATGGCAAATTTGTTGTGTTAA

Upstream 100 bases:

>100_bases
TGTGTAGTAATATAAAACTATGTATTTTTCGTCATTAACTAAATATTAACCTGCACACTGTTGAATGCTACGCTCATCGG
GGAAATAATGAGGTAAAGAT

Downstream 100 bases:

>100_bases
GCCATGGAATCTTTAGTGCTCGCAAACCCTTTAAGGAGACTTATAGCTAGTCTCATAGATATCATAATAATATCAGTGGG
GACCATACCAATAACGTTTG

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MQPPVAVMFDWCNTIVKTDKLDFAILSRVLTSMNLPHIDLSDIPSNKVELYLSCALGYRWKEAVNMYEKIAGMNINARSM
SPNENVLELLEFLSHHKINMGIVSNKRGPNLRNEVQNLGLGKYFSVILGSGDTAENKPSPEPIFAALDIMGIAPSERVFF
VGDSDSDIESAKRARCVPIAYDNTSIEGVLSFANFKDLGNFMANLLC

Sequences:

>Translated_207_residues
MQPPVAVMFDWCNTIVKTDKLDFAILSRVLTSMNLPHIDLSDIPSNKVELYLSCALGYRWKEAVNMYEKIAGMNINARSM
SPNENVLELLEFLSHHKINMGIVSNKRGPNLRNEVQNLGLGKYFSVILGSGDTAENKPSPEPIFAALDIMGIAPSERVFF
VGDSDSDIESAKRARCVPIAYDNTSIEGVLSFANFKDLGNFMANLLC
>Mature_207_residues
MQPPVAVMFDWCNTIVKTDKLDFAILSRVLTSMNLPHIDLSDIPSNKVELYLSCALGYRWKEAVNMYEKIAGMNINARSM
SPNENVLELLEFLSHHKINMGIVSNKRGPNLRNEVQNLGLGKYFSVILGSGDTAENKPSPEPIFAALDIMGIAPSERVFF
VGDSDSDIESAKRARCVPIAYDNTSIEGVLSFANFKDLGNFMANLLC

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=92, Percent_Identity=39.1304347826087, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 22937; Mature: 22937

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPPVAVMFDWCNTIVKTDKLDFAILSRVLTSMNLPHIDLSDIPSNKVELYLSCALGYRW
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHH
KEAVNMYEKIAGMNINARSMSPNENVLELLEFLSHHKINMGIVSNKRGPNLRNEVQNLGL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCC
GKYFSVILGSGDTAENKPSPEPIFAALDIMGIAPSERVFFVGDSDSDIESAKRARCVPIA
CHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCEEEEEE
YDNTSIEGVLSFANFKDLGNFMANLLC
ECCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQPPVAVMFDWCNTIVKTDKLDFAILSRVLTSMNLPHIDLSDIPSNKVELYLSCALGYRW
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCHH
KEAVNMYEKIAGMNINARSMSPNENVLELLEFLSHHKINMGIVSNKRGPNLRNEVQNLGL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCC
GKYFSVILGSGDTAENKPSPEPIFAALDIMGIAPSERVFFVGDSDSDIESAKRARCVPIA
CHHHHHEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCEEEEEE
YDNTSIEGVLSFANFKDLGNFMANLLC
ECCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]