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The map label for this gene is aspS

Identifier: 88606895

GI number: 88606895

Start: 189372

End: 191168

Strand: Reverse

Name: aspS

Synonym: APH_0190

Alternate gene names: 88606895

Gene position: 191168-189372 (Counterclockwise)

Preceding gene: 88606741

Following gene: 88607019

Centisome position: 12.99

GC content: 44.02

Gene sequence:

>1797_bases
ATGAACATGTACAGAACACATTTGTGTAACGCACTTAATTTATCTCATGTAGGAGAAGAGGTAACGCTGTCAGGTTGGAT
ATTTAGAAAACGTGATCACGGTGGTATTTTATTCATCGATTTGAGGGACTTCTATGGAATAACACAGTTGGTGTTAAATG
AGTCCTCAGATCAAGAACTGTTTAATTACGCGCGTTCCATAGGGTTAGAGAGCGTTATCACTGTAAAGGGAACTGTAGCA
GCACGTAGCGAAGACACAATCAATACCTCGCTAAGTACCGGTCACGTTGAAGTGGCTGTGCATACCCTAGTGACAGAGTC
TGCTGCCGATGCATTGCCTATACACGTGCCCACAAGTACCAACCATCCTGAAGACTTGCGGTTGAAATACAGGTTTTTGG
ATCTGCGGTGCGACAAGGTAAAAAGCAACATGTTACTACGTTCTGCTGTAATTTCTGAGATGCGGAAAGCAATGGAAAGC
CGCGGGTTTATAGAGGTGCAAACACCGATATTGACAGCGTCGTCTCCCGAAGGAGCAAGAGATTATCTCGTGCCTAGCAG
AGTGCATGCTGGCAAGTTTTACGCGCTACCTCAGGCTCCACAGATATTTAAGCAGCTGCTAATGGCAAGCGGTTTTGATA
AGTATTTCCAGATTGCTCCATGTTTCCGTGATGAAGATGCGCGTGCAGATAGATCTCCTGGCGAATTTTATCAGCTCGAC
GTTGAAATGTCCTTCGTCACACAAGAAGATGTATTTTCAGTCATGGAACCCGTGTTGCGCGATATCTTTACGAAATTTGC
TGGCAACAGGAGCGTATCACCCACATTCCCGAGAATTACCTACAAGGACGCTATGCTGCGTTATGGGACTGATAAGCCTG
ATTTGAGAAACCCCATAATAATAGCAGACGTATCTGAAGTGTTCCTGCGCTCTAACTTTAAGACATTTCAAGAGGGAGTT
GCTCGTGGCATGGTTGTGCGGGCTATACCAGCTCCTAAAACTTCAGAACACCCTCGCAGCTTTTTTGATAGCAAGGTGGA
ATATGCCAAAAAGATAGGAGCTCGGGGATTAGGCTACATAACATTTTCTACAGATAACACCGTAAAAGGTCCAGTTGCTA
AATTTTTGAGTGACACCGAATTAGCAAATATCCAAACACTCGCTGGTGTCGGACCAGGAGATAGCGTATTTTTTGTCTCT
GACGCTGCAGACAAAGCTGCAGAACTCTCAGGCAGTGTTAGGGAGTTACTGGGAACAGAATTGAATCTGATAGAGAAGGA
TACTTTTAAATTCTGTTGGATAGTAGATTTTCCTTATTTCCAATACGAAAACGGTAAGCTAGCATTTTCACACAACCCAT
TCTCTATGCCTCAGGGTGGGTTAGATGCTCTTTCTAGCTCCAACCCTCTAGATATAGTCGCCTACCAATATGACATAGTG
TGTAACGGTATTGAGATATCCAGCGGTGCCATAAGAAATCATAAACTTGACATATTGTATAAGGCATTCTCCATGGTTGG
TTATAGCCCTGAAGCAGTAGATGCAGAATTTGGGGCTCTAACGCGTGCTTTTAGGTTTGGTGTTCCTCCACACGGAGGTA
TTGCACCAGGTGTAGACAGGATAGTAATGCTGTTGGCTGATGTGCCCAACATTAGAGAAATTATATACTTCCCTCTAACA
CAAATGGGAGAAGATTTGCTCATGGGAGCTCCATCTGAAGTTAATCAAAGCCACCTGAAAGAGCTGTCTCTAGCATTAAA
TATTACCCCAAAAGCTGCAGGAAAAACCAGCTCTTAG

Upstream 100 bases:

>100_bases
TACAGATTTGCAATCCTCCAATACATTAGAAATTTTCCGTAGCATTAGACAACATTGTGATAGAAAATGGGAGTGGGATT
GGTCTTCCAGAGTATTTCGC

Downstream 100 bases:

>100_bases
GGGAATAAGACATCATCTACTGAAGGCCTATATCACCAATGTGGAAACATGCGGGGAATATAGGCCCAAACGTCTCCGCA
TAATGTGCGACTAACATATG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MNMYRTHLCNALNLSHVGEEVTLSGWIFRKRDHGGILFIDLRDFYGITQLVLNESSDQELFNYARSIGLESVITVKGTVA
ARSEDTINTSLSTGHVEVAVHTLVTESAADALPIHVPTSTNHPEDLRLKYRFLDLRCDKVKSNMLLRSAVISEMRKAMES
RGFIEVQTPILTASSPEGARDYLVPSRVHAGKFYALPQAPQIFKQLLMASGFDKYFQIAPCFRDEDARADRSPGEFYQLD
VEMSFVTQEDVFSVMEPVLRDIFTKFAGNRSVSPTFPRITYKDAMLRYGTDKPDLRNPIIIADVSEVFLRSNFKTFQEGV
ARGMVVRAIPAPKTSEHPRSFFDSKVEYAKKIGARGLGYITFSTDNTVKGPVAKFLSDTELANIQTLAGVGPGDSVFFVS
DAADKAAELSGSVRELLGTELNLIEKDTFKFCWIVDFPYFQYENGKLAFSHNPFSMPQGGLDALSSSNPLDIVAYQYDIV
CNGIEISSGAIRNHKLDILYKAFSMVGYSPEAVDAEFGALTRAFRFGVPPHGGIAPGVDRIVMLLADVPNIREIIYFPLT
QMGEDLLMGAPSEVNQSHLKELSLALNITPKAAGKTSS

Sequences:

>Translated_598_residues
MNMYRTHLCNALNLSHVGEEVTLSGWIFRKRDHGGILFIDLRDFYGITQLVLNESSDQELFNYARSIGLESVITVKGTVA
ARSEDTINTSLSTGHVEVAVHTLVTESAADALPIHVPTSTNHPEDLRLKYRFLDLRCDKVKSNMLLRSAVISEMRKAMES
RGFIEVQTPILTASSPEGARDYLVPSRVHAGKFYALPQAPQIFKQLLMASGFDKYFQIAPCFRDEDARADRSPGEFYQLD
VEMSFVTQEDVFSVMEPVLRDIFTKFAGNRSVSPTFPRITYKDAMLRYGTDKPDLRNPIIIADVSEVFLRSNFKTFQEGV
ARGMVVRAIPAPKTSEHPRSFFDSKVEYAKKIGARGLGYITFSTDNTVKGPVAKFLSDTELANIQTLAGVGPGDSVFFVS
DAADKAAELSGSVRELLGTELNLIEKDTFKFCWIVDFPYFQYENGKLAFSHNPFSMPQGGLDALSSSNPLDIVAYQYDIV
CNGIEISSGAIRNHKLDILYKAFSMVGYSPEAVDAEFGALTRAFRFGVPPHGGIAPGVDRIVMLLADVPNIREIIYFPLT
QMGEDLLMGAPSEVNQSHLKELSLALNITPKAAGKTSS
>Mature_598_residues
MNMYRTHLCNALNLSHVGEEVTLSGWIFRKRDHGGILFIDLRDFYGITQLVLNESSDQELFNYARSIGLESVITVKGTVA
ARSEDTINTSLSTGHVEVAVHTLVTESAADALPIHVPTSTNHPEDLRLKYRFLDLRCDKVKSNMLLRSAVISEMRKAMES
RGFIEVQTPILTASSPEGARDYLVPSRVHAGKFYALPQAPQIFKQLLMASGFDKYFQIAPCFRDEDARADRSPGEFYQLD
VEMSFVTQEDVFSVMEPVLRDIFTKFAGNRSVSPTFPRITYKDAMLRYGTDKPDLRNPIIIADVSEVFLRSNFKTFQEGV
ARGMVVRAIPAPKTSEHPRSFFDSKVEYAKKIGARGLGYITFSTDNTVKGPVAKFLSDTELANIQTLAGVGPGDSVFFVS
DAADKAAELSGSVRELLGTELNLIEKDTFKFCWIVDFPYFQYENGKLAFSHNPFSMPQGGLDALSSSNPLDIVAYQYDIV
CNGIEISSGAIRNHKLDILYKAFSMVGYSPEAVDAEFGALTRAFRFGVPPHGGIAPGVDRIVMLLADVPNIREIIYFPLT
QMGEDLLMGAPSEVNQSHLKELSLALNITPKAAGKTSS

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=588, Percent_Identity=35.5442176870748, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI45439306, Length=325, Percent_Identity=25.8461538461538, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI226491502, Length=252, Percent_Identity=24.6031746031746, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1788173, Length=592, Percent_Identity=41.3851351351351, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1790571, Length=301, Percent_Identity=25.5813953488372, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789256, Length=306, Percent_Identity=25.1633986928105, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI32566633, Length=607, Percent_Identity=34.4316309719934, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17551876, Length=280, Percent_Identity=26.4285714285714, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71984122, Length=306, Percent_Identity=24.5098039215686, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994340, Length=224, Percent_Identity=25.4464285714286, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=224, Percent_Identity=25.4464285714286, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=224, Percent_Identity=25.4464285714286, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=645, Percent_Identity=30.077519379845, Blast_Score=243, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6323011, Length=244, Percent_Identity=26.6393442622951, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321807, Length=292, Percent_Identity=26.027397260274, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24584738, Length=597, Percent_Identity=35.3433835845896, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI17136276, Length=241, Percent_Identity=27.8008298755187, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921528, Length=285, Percent_Identity=25.6140350877193, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_ANAPZ (Q2GLD8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_504813.1
- HSSP:   O57980
- ProteinModelPortal:   Q2GLD8
- SMR:   Q2GLD8
- STRING:   Q2GLD8
- GeneID:   3930308
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0190
- NMPDR:   fig|212042.5.peg.183
- TIGR:   APH_0190
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   APHA212042:APH_0190-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66205; Mature: 66205

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMYRTHLCNALNLSHVGEEVTLSGWIFRKRDHGGILFIDLRDFYGITQLVLNESSDQEL
CCCHHHHHHCCCCHHHCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHCCCCHHHH
FNYARSIGLESVITVKGTVAARSEDTINTSLSTGHVEVAVHTLVTESAADALPIHVPTST
HHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEEEEHHHHCCCCCEEEEECCCC
NHPEDLRLKYRFLDLRCDKVKSNMLLRSAVISEMRKAMESRGFIEVQTPILTASSPEGAR
CCCHHEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCC
DYLVPSRVHAGKFYALPQAPQIFKQLLMASGFDKYFQIAPCFRDEDARADRSPGEFYQLD
CCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEE
VEMSFVTQEDVFSVMEPVLRDIFTKFAGNRSVSPTFPRITYKDAMLRYGTDKPDLRNPII
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCCEE
IADVSEVFLRSNFKTFQEGVARGMVVRAIPAPKTSEHPRSFFDSKVEYAKKIGARGLGYI
EECHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
TFSTDNTVKGPVAKFLSDTELANIQTLAGVGPGDSVFFVSDAADKAAELSGSVRELLGTE
EECCCCCCCHHHHHHHCCCHHCCHHHEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
LNLIEKDTFKFCWIVDFPYFQYENGKLAFSHNPFSMPQGGLDALSSSNPLDIVAYQYDIV
CCEEECCCEEEEEEEECCCEEECCCEEEECCCCCCCCCCCHHHHCCCCCEEEEEEEEEEE
CNGIEISSGAIRNHKLDILYKAFSMVGYSPEAVDAEFGALTRAFRFGVPPHGGIAPGVDR
ECCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADVPNIREIIYFPLTQMGEDLLMGAPSEVNQSHLKELSLALNITPKAAGKTSS
HHHHHHCCCCHHHHEEECHHHCCCHHHCCCCCCCCHHHHHHHHEEEEECCCCCCCCCC
>Mature Secondary Structure
MNMYRTHLCNALNLSHVGEEVTLSGWIFRKRDHGGILFIDLRDFYGITQLVLNESSDQEL
CCCHHHHHHCCCCHHHCCCEEEEEEEEEEEECCCCEEEEECHHHHHHHHHHHCCCCHHHH
FNYARSIGLESVITVKGTVAARSEDTINTSLSTGHVEVAVHTLVTESAADALPIHVPTST
HHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCEEEEEEEEHHHHCCCCCEEEEECCCC
NHPEDLRLKYRFLDLRCDKVKSNMLLRSAVISEMRKAMESRGFIEVQTPILTASSPEGAR
CCCHHEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCC
DYLVPSRVHAGKFYALPQAPQIFKQLLMASGFDKYFQIAPCFRDEDARADRSPGEFYQLD
CCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEE
VEMSFVTQEDVFSVMEPVLRDIFTKFAGNRSVSPTFPRITYKDAMLRYGTDKPDLRNPII
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHCCCCCCCCCCCEE
IADVSEVFLRSNFKTFQEGVARGMVVRAIPAPKTSEHPRSFFDSKVEYAKKIGARGLGYI
EECHHHHHHHCCHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
TFSTDNTVKGPVAKFLSDTELANIQTLAGVGPGDSVFFVSDAADKAAELSGSVRELLGTE
EECCCCCCCHHHHHHHCCCHHCCHHHEECCCCCCCEEEEECCHHHHHHHHHHHHHHHCCC
LNLIEKDTFKFCWIVDFPYFQYENGKLAFSHNPFSMPQGGLDALSSSNPLDIVAYQYDIV
CCEEECCCEEEEEEEECCCEEECCCEEEECCCCCCCCCCCHHHHCCCCCEEEEEEEEEEE
CNGIEISSGAIRNHKLDILYKAFSMVGYSPEAVDAEFGALTRAFRFGVPPHGGIAPGVDR
ECCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADVPNIREIIYFPLTQMGEDLLMGAPSEVNQSHLKELSLALNITPKAAGKTSS
HHHHHHCCCCHHHHEEECHHHCCCHHHCCCCCCCCHHHHHHHHEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA