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The map label for this gene is virB4 [H]

Identifier: 88606981

GI number: 88606981

Start: 432110

End: 432943

Strand: Reverse

Name: virB4 [H]

Synonym: APH_0419

Alternate gene names: 88606981

Gene position: 432943-432110 (Counterclockwise)

Preceding gene: 88606704

Following gene: 88607552

Centisome position: 29.43

GC content: 41.61

Gene sequence:

>834_bases
ATGGAGTGGTTTAAGTTATTGGCTACTACTCTGTCAGATAAGCTTACGCCTGACGATATATTGCGCATTGATGATGCTAT
AGAGGGTAATTTCAAGCTCAAAAAGGAAGACAGAAAGTTACGTAATCTGGTTCCTTTTTTGGGTATAGGTGGAGAAGATA
CTCTAGCGAGTCGCATGATGATGTGGCATTCAGAAGGGTCGCACGCGGCGCTTTTTGATAATGACGAAGATGTATTAGAC
TTCACTAAGTCTAGAGTATTTGGATTTGAGATGGGATATTTGCTAAAAGATCCCATGGCTCTTGCTCCGACGCTTTTGTA
TTTGTTTCACAAGATAAGTATTTCTCTCGATGGAACTCCTTCTATGATTATCTTGGACGAAGCGTGGGCGCTGATAGATA
ATCCTGTTTTTGCGCCTAGGATTAAAGACTGGCTAAAAGTTCTTAGGAAGCTCAATACTTTTGTCATATTTGCGACACAG
AGTGTTGAGGATGCTAGTAAAAGTCAGATAAGTGATACCTTAGTTCAGCAGACTGCCACTCAGATATTTTTGCCTAATCT
TAAGGCTACTAGTGCTTACAGGGATGTTTTCATGCTGACTGAGCGGGAGTATTCTTTAATAAAATATACAGATCCTGGCA
CTAGGTTTTTCCTAGTCAAGCAGGGGGTCAGTGCTGTTGTGGCCAGGATAGATTTACGAGGGCTCGATGACACGATAAAC
GTTTTGTCAGGTAGGGCCGAGACCGTTGCCATACTTCGTGAAATTATGGAAGAAGTAGGGGATGATCCAAATGTGTGGTT
GCCTATTTTTTATCAAAGGGTGAAAAATGCATAG

Upstream 100 bases:

>100_bases
CAGGGCTTTGAAGGGGGTATACAGCATTATAGAGACAAGAGGCTCCACTGGGTTAAATCCGCTGCACTTAGACGATACTC
CTGACAATAGGGGTTTTCTC

Downstream 100 bases:

>100_bases
GGTAGCAAGGGCATTGGTATTTTTGATGTTTCTTGTTGTTACAGTTCCTTTAACGTCATACGCGGCGGGCGATGCTACTG
CTAGTACACCAACAGAGGAA

Product: type IV secretion system protein VirB4, truncation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MEWFKLLATTLSDKLTPDDILRIDDAIEGNFKLKKEDRKLRNLVPFLGIGGEDTLASRMMMWHSEGSHAALFDNDEDVLD
FTKSRVFGFEMGYLLKDPMALAPTLLYLFHKISISLDGTPSMIILDEAWALIDNPVFAPRIKDWLKVLRKLNTFVIFATQ
SVEDASKSQISDTLVQQTATQIFLPNLKATSAYRDVFMLTEREYSLIKYTDPGTRFFLVKQGVSAVVARIDLRGLDDTIN
VLSGRAETVAILREIMEEVGDDPNVWLPIFYQRVKNA

Sequences:

>Translated_277_residues
MEWFKLLATTLSDKLTPDDILRIDDAIEGNFKLKKEDRKLRNLVPFLGIGGEDTLASRMMMWHSEGSHAALFDNDEDVLD
FTKSRVFGFEMGYLLKDPMALAPTLLYLFHKISISLDGTPSMIILDEAWALIDNPVFAPRIKDWLKVLRKLNTFVIFATQ
SVEDASKSQISDTLVQQTATQIFLPNLKATSAYRDVFMLTEREYSLIKYTDPGTRFFLVKQGVSAVVARIDLRGLDDTIN
VLSGRAETVAILREIMEEVGDDPNVWLPIFYQRVKNA
>Mature_277_residues
MEWFKLLATTLSDKLTPDDILRIDDAIEGNFKLKKEDRKLRNLVPFLGIGGEDTLASRMMMWHSEGSHAALFDNDEDVLD
FTKSRVFGFEMGYLLKDPMALAPTLLYLFHKISISLDGTPSMIILDEAWALIDNPVFAPRIKDWLKVLRKLNTFVIFATQ
SVEDASKSQISDTLVQQTATQIFLPNLKATSAYRDVFMLTEREYSLIKYTDPGTRFFLVKQGVSAVVARIDLRGLDDTIN
VLSGRAETVAILREIMEEVGDDPNVWLPIFYQRVKNA

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 31475; Mature: 31475

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWFKLLATTLSDKLTPDDILRIDDAIEGNFKLKKEDRKLRNLVPFLGIGGEDTLASRMM
CHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHCCCCCHHHHHHHH
MWHSEGSHAALFDNDEDVLDFTKSRVFGFEMGYLLKDPMALAPTLLYLFHKISISLDGTP
HHCCCCCEEEEECCCHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHHHHHHEEEECCCC
SMIILDEAWALIDNPVFAPRIKDWLKVLRKLNTFVIFATQSVEDASKSQISDTLVQQTAT
CEEEEECHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
QIFLPNLKATSAYRDVFMLTEREYSLIKYTDPGTRFFLVKQGVSAVVARIDLRGLDDTIN
HHCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
VLSGRAETVAILREIMEEVGDDPNVWLPIFYQRVKNA
HHCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCC
>Mature Secondary Structure
MEWFKLLATTLSDKLTPDDILRIDDAIEGNFKLKKEDRKLRNLVPFLGIGGEDTLASRMM
CHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEECHHHHHHHHCCCHHCCCCCHHHHHHHH
MWHSEGSHAALFDNDEDVLDFTKSRVFGFEMGYLLKDPMALAPTLLYLFHKISISLDGTP
HHCCCCCEEEEECCCHHHHHHHHHHHHHEEHHHHHCCCHHHHHHHHHHHHHHEEEECCCC
SMIILDEAWALIDNPVFAPRIKDWLKVLRKLNTFVIFATQSVEDASKSQISDTLVQQTAT
CEEEEECHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHH
QIFLPNLKATSAYRDVFMLTEREYSLIKYTDPGTRFFLVKQGVSAVVARIDLRGLDDTIN
HHCCCCCCHHHHHHHHHEEECCCCEEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCHHHH
VLSGRAETVAILREIMEEVGDDPNVWLPIFYQRVKNA
HHCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]