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The map label for this gene is prfA

Identifier: 88606704

GI number: 88606704

Start: 433514

End: 434593

Strand: Reverse

Name: prfA

Synonym: APH_0420

Alternate gene names: 88606704

Gene position: 434593-433514 (Counterclockwise)

Preceding gene: 88607906

Following gene: 88606981

Centisome position: 29.54

GC content: 43.52

Gene sequence:

>1080_bases
ATGAGCTTTGAAAGCACCTTTGAGGGGTTGTGTGAAAAATTTCGCATTTTGAAACAACAGCTTTCCGCCCCCGAGACACT
TGGAACCCAAGCTTTTGTCGTGGCATCGCGTGAGTATTCAGACTTACTCCCTATCATGTCTTTGATCGAAAAATACAAAT
CTACGCAGAAGGAAATTGCGGAATTGGAAGAGTTAGTAAATAGCGCCAGTACTGACCCTGAACTCCGCAGCCTAGCTAAA
GATGAGTCACACATCAAACAAAAACTCTTACCTAAGCTCAGACACGAACTACAACTATCGCTGCTGCCCAAGGATAGAGA
CGATTCTCGTAATGCAATTTTAGAGATTAGAGCAGGTACAGGGGGCGAAGAGGCCGCGCTATTCGTGGGGAATCTATATC
GTATGTACCTTAAGTATGCTGAAAGAAAAAATTGGAAGGTGGAAACCATTAACATTTCCACAACAGGAATCGGCGGCTAT
AAAGAGGCATCGTTTAGTATAGGCGGTAAAGATGTTTTTGCGCGGCTGAAGTTCGAATCCGGCGTGCACAGAGTACAGAG
AGTGCCTGAAACTGAATCATCAGGTAGGTTGCACACATCTGCTGCAACTGTGGCTGTTTTACCTGAAGTTGAGGAAGTAG
ATCTAAAAATTGATGAAAAAGATCTCAGAATAGACGTTTACCGTTCTAGTGGACCTGGAGGACAATCAGTAAATACCACA
GACAGTGCTGTCAGAATAACACATATCCCTACCGGCATAGTGGTAATCCAGCAGGATGAAAAATCGCAACATAAGAATAA
GAGTAAAGCATTAAAAGTGCTCAGGGCTAGGTTATATAACCTAGAAAAACAAAAAAGGGAGGAGGAGATCTCTAAAATGC
GTAAAAGTCAGATAGGTTCCGGAGACCGGTCCGAGCGCATTAGGACCTATAATTTTCTTCAGTCCCGCATCACTGACCAC
AGGATCAATTTGACGTCGTACAGGCTCGATTACGTAATGAAGGAAGGAGACTTAGACGAGTTCATAGATGCTCTAGTCGC
AGATGATCAAGCTAATAAGCTGAAACAAATAACGCACTGA

Upstream 100 bases:

>100_bases
AAAAATTTACAATTCCAGATACGGGTGTTTGTAGCAGCGGTATGCTTCTGTTACTTACATACTTACCGTTTCTGGGCGTT
TTAGAGGTACAGGTAGAATT

Downstream 100 bases:

>100_bases
AGCCTCGTTTCATACTATGGACCAACTGTTTGGGATTTATTCCCTTAGGGTACGTAGTAATAAGACGGTCCTTAAGTTGT
ATCTCTATGTACAGGGTGCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSFESTFEGLCEKFRILKQQLSAPETLGTQAFVVASREYSDLLPIMSLIEKYKSTQKEIAELEELVNSASTDPELRSLAK
DESHIKQKLLPKLRHELQLSLLPKDRDDSRNAILEIRAGTGGEEAALFVGNLYRMYLKYAERKNWKVETINISTTGIGGY
KEASFSIGGKDVFARLKFESGVHRVQRVPETESSGRLHTSAATVAVLPEVEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
DSAVRITHIPTGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKREEEISKMRKSQIGSGDRSERIRTYNFLQSRITDH
RINLTSYRLDYVMKEGDLDEFIDALVADDQANKLKQITH

Sequences:

>Translated_359_residues
MSFESTFEGLCEKFRILKQQLSAPETLGTQAFVVASREYSDLLPIMSLIEKYKSTQKEIAELEELVNSASTDPELRSLAK
DESHIKQKLLPKLRHELQLSLLPKDRDDSRNAILEIRAGTGGEEAALFVGNLYRMYLKYAERKNWKVETINISTTGIGGY
KEASFSIGGKDVFARLKFESGVHRVQRVPETESSGRLHTSAATVAVLPEVEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
DSAVRITHIPTGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKREEEISKMRKSQIGSGDRSERIRTYNFLQSRITDH
RINLTSYRLDYVMKEGDLDEFIDALVADDQANKLKQITH
>Mature_358_residues
SFESTFEGLCEKFRILKQQLSAPETLGTQAFVVASREYSDLLPIMSLIEKYKSTQKEIAELEELVNSASTDPELRSLAKD
ESHIKQKLLPKLRHELQLSLLPKDRDDSRNAILEIRAGTGGEEAALFVGNLYRMYLKYAERKNWKVETINISTTGIGGYK
EASFSIGGKDVFARLKFESGVHRVQRVPETESSGRLHTSAATVAVLPEVEEVDLKIDEKDLRIDVYRSSGPGGQSVNTTD
SAVRITHIPTGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKREEEISKMRKSQIGSGDRSERIRTYNFLQSRITDHR
INLTSYRLDYVMKEGDLDEFIDALVADDQANKLKQITH

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=306, Percent_Identity=49.0196078431373, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI34577120, Length=328, Percent_Identity=42.6829268292683, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=47.3429951690821, Blast_Score=200, Evalue=2e-51,
Organism=Escherichia coli, GI1787462, Length=359, Percent_Identity=51.2534818941504, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI2367172, Length=311, Percent_Identity=39.2282958199357, Blast_Score=202, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=277, Percent_Identity=40.4332129963899, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=359, Percent_Identity=41.7827298050139, Blast_Score=235, Evalue=7e-63,
Organism=Drosophila melanogaster, GI19921226, Length=323, Percent_Identity=43.0340557275542, Blast_Score=240, Evalue=8e-64,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_ANAPZ (Q2GKS8)

Other databases:

- EMBL:   CP000235
- RefSeq:   YP_505023.1
- ProteinModelPortal:   Q2GKS8
- SMR:   Q2GKS8
- STRING:   Q2GKS8
- GeneID:   3929978
- GenomeReviews:   CP000235_GR
- KEGG:   aph:APH_0420
- NMPDR:   fig|212042.5.peg.406
- TIGR:   APH_0420
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q2GKS8
- ProtClustDB:   PRK00591
- BioCyc:   APHA212042:APH_0420-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40718; Mature: 40587

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFESTFEGLCEKFRILKQQLSAPETLGTQAFVVASREYSDLLPIMSLIEKYKSTQKEIA
CCCHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ELEELVNSASTDPELRSLAKDESHIKQKLLPKLRHELQLSLLPKDRDDSRNAILEIRAGT
HHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCC
GGEEAALFVGNLYRMYLKYAERKNWKVETINISTTGIGGYKEASFSIGGKDVFARLKFES
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHEEHHHHH
GVHRVQRVPETESSGRLHTSAATVAVLPEVEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
HHHHHHHCCCCCCCCCEEECCEEEEECCCHHHCCCEECCCCCEEEEEECCCCCCCCCCCC
DSAVRITHIPTGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKREEEISKMRKSQIGS
CCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFLQSRITDHRINLTSYRLDYVMKEGDLDEFIDALVADDQANKLKQITH
CCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure 
SFESTFEGLCEKFRILKQQLSAPETLGTQAFVVASREYSDLLPIMSLIEKYKSTQKEIA
CCHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
ELEELVNSASTDPELRSLAKDESHIKQKLLPKLRHELQLSLLPKDRDDSRNAILEIRAGT
HHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCC
GGEEAALFVGNLYRMYLKYAERKNWKVETINISTTGIGGYKEASFSIGGKDVFARLKFES
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHEEHHHHH
GVHRVQRVPETESSGRLHTSAATVAVLPEVEEVDLKIDEKDLRIDVYRSSGPGGQSVNTT
HHHHHHHCCCCCCCCCEEECCEEEEECCCHHHCCCEECCCCCEEEEEECCCCCCCCCCCC
DSAVRITHIPTGIVVIQQDEKSQHKNKSKALKVLRARLYNLEKQKREEEISKMRKSQIGS
CCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GDRSERIRTYNFLQSRITDHRINLTSYRLDYVMKEGDLDEFIDALVADDQANKLKQITH
CCHHHHHHHHHHHHHHHHHCEEEHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA