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The map label for this gene is dnaA [H]

Identifier: 88606838

GI number: 88606838

Start: 501807

End: 503186

Strand: Direct

Name: dnaA [H]

Synonym: APH_0474

Alternate gene names: 88606838

Gene position: 501807-503186 (Clockwise)

Preceding gene: 88607336

Following gene: 88607531

Centisome position: 34.11

GC content: 44.64

Gene sequence:

>1380_bases
ATGGATAGGGATAGTACCTATGACGGGCATTTGTCATGGCAGAGTGTTCAGAAAAAGTTACGGGATTTCTATGGCAGTGC
AGCATATGATAGCTGGCTGAGTGCTTTGGTGTATTCTGGTTATGAAAACGGTAGAGTTATTCTTTTGGTGCCTACGCGGT
TCATAAAGGAGTGGATTCTCGTGCATTACCTGGAGAAAATCCTTGAGTATTGGCGTGGGGAGAATAGCGCTGTTTACTGT
ATTGACATATGTGTTGCAGATGGTGCTGAGAGTAGCGTTCTGCAGGGTAATAGGGGTGTTTCGGTTAGGTCTACAGAGGC
GCAGGAAGTTTCTTCTGTTAGTACATATGAACATTTGAGTTCGCCTTTGGATCCGAGGTTTACGTTTGATAATTTTGTTG
TAGGGAAGCCGAATGAGCTAGCCTTTGCTGCGGCAAGGCGTGTAGCCGAGTCTTCGGAGCCAATTCCAGGGAGTAATCCG
TTGTTTCTATATGGTGGTGTAGGTTTGGGTAAGACGCATCTTATGCATGCGATTGCCTGGTACGCGCTTGGGTCTCCTAG
GAAGAGGAAGATAGCTTACCTATCTGCTGAGAAGTTTATGTATCAGTACGTTACGGCGCTGCGAAGTAAGGACATCATGT
TGTTTAAAGAGCAGTTTCGTTCAGTAGATGTATTGATGGTGGATGATGTGCAGTTCATTAGTGGCAAGGACAGCACGCAA
GAAGAGTTCTTTCATACATTTAACGCGCTTATAGATCAGAGTAAGCAGTTGGTGATTTCTGCTGACAGGTCTCCTAGTGA
TCTAGATGGGGTAGAAGATAGGATTAAGTCTCGATTGGGATGGGGGTTAGTAGCTGATATTAATGAGACTACTTTTGAGC
TCAGGCTGGGTATATTGCAGCTGAAAGTCGAGAAGATGGGCATACACGTGCCAGATGAGGTACTTGAATTTCTTGCTAAA
AACATTAAGTCTAATATCAGGGAGCTGGAAGGTGCTTTAAATAAAGTGGTCGCGCATTCTTCGTTGGTTGGGAGTAGTGT
TACTGTTGAGCTTGCGAGTGACATTTTGTCTGATTTGTTACGTGCGAATCATAGATTAGTGACCATAGACGCTATTCAGA
AGAAGGTGGCGGAGTTTTTTGGCATCAGGCTAGAAGACATGTATTCTGCGAGGAGGCTGAGGGCTCTTGCTAGACCAAGG
CAGGTAGCCATGTATTTATCGAAAAGGCTGACGCAGAAGAGTTTGCCTGACATAGGCAAAAGCTTTGGTGGTAGGGATCA
TGCGACTGTTATTCATGCTGTCAAGCAGGTGGAAAAGTTTATAGATACTGATTCAAAGCTTGCGGGTGATATAAGTCTAT
TGATAAGGATGTTGAGGTAG

Upstream 100 bases:

>100_bases
TCTATTGTTGCGGAGGTAGCGATAGAGTAAGTCAAAGTTGTGATTTGGGTTTATCAAAATGATGGGTAATTCCGGGGCTA
CTGATTGTAATGTGGGTACT

Downstream 100 bases:

>100_bases
GTCGGCGTAACGACAGAAGTGTGTGTCCTATGGTGGAGATATTGGGTGTATAGCTTTTGAAAAGATGTGCATGCGCTGGT
ATGTAGTAGTTTCAGGCGTA

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MDRDSTYDGHLSWQSVQKKLRDFYGSAAYDSWLSALVYSGYENGRVILLVPTRFIKEWILVHYLEKILEYWRGENSAVYC
IDICVADGAESSVLQGNRGVSVRSTEAQEVSSVSTYEHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESSEPIPGSNP
LFLYGGVGLGKTHLMHAIAWYALGSPRKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDSTQ
EEFFHTFNALIDQSKQLVISADRSPSDLDGVEDRIKSRLGWGLVADINETTFELRLGILQLKVEKMGIHVPDEVLEFLAK
NIKSNIRELEGALNKVVAHSSLVGSSVTVELASDILSDLLRANHRLVTIDAIQKKVAEFFGIRLEDMYSARRLRALARPR
QVAMYLSKRLTQKSLPDIGKSFGGRDHATVIHAVKQVEKFIDTDSKLAGDISLLIRMLR

Sequences:

>Translated_459_residues
MDRDSTYDGHLSWQSVQKKLRDFYGSAAYDSWLSALVYSGYENGRVILLVPTRFIKEWILVHYLEKILEYWRGENSAVYC
IDICVADGAESSVLQGNRGVSVRSTEAQEVSSVSTYEHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESSEPIPGSNP
LFLYGGVGLGKTHLMHAIAWYALGSPRKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDSTQ
EEFFHTFNALIDQSKQLVISADRSPSDLDGVEDRIKSRLGWGLVADINETTFELRLGILQLKVEKMGIHVPDEVLEFLAK
NIKSNIRELEGALNKVVAHSSLVGSSVTVELASDILSDLLRANHRLVTIDAIQKKVAEFFGIRLEDMYSARRLRALARPR
QVAMYLSKRLTQKSLPDIGKSFGGRDHATVIHAVKQVEKFIDTDSKLAGDISLLIRMLR
>Mature_459_residues
MDRDSTYDGHLSWQSVQKKLRDFYGSAAYDSWLSALVYSGYENGRVILLVPTRFIKEWILVHYLEKILEYWRGENSAVYC
IDICVADGAESSVLQGNRGVSVRSTEAQEVSSVSTYEHLSSPLDPRFTFDNFVVGKPNELAFAAARRVAESSEPIPGSNP
LFLYGGVGLGKTHLMHAIAWYALGSPRKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDSTQ
EEFFHTFNALIDQSKQLVISADRSPSDLDGVEDRIKSRLGWGLVADINETTFELRLGILQLKVEKMGIHVPDEVLEFLAK
NIKSNIRELEGALNKVVAHSSLVGSSVTVELASDILSDLLRANHRLVTIDAIQKKVAEFFGIRLEDMYSARRLRALARPR
QVAMYLSKRLTQKSLPDIGKSFGGRDHATVIHAVKQVEKFIDTDSKLAGDISLLIRMLR

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=472, Percent_Identity=40.2542372881356, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI226510964, Length=231, Percent_Identity=25.5411255411255, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 51654; Mature: 51654

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRDSTYDGHLSWQSVQKKLRDFYGSAAYDSWLSALVYSGYENGRVILLVPTRFIKEWIL
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHH
VHYLEKILEYWRGENSAVYCIDICVADGAESSVLQGNRGVSVRSTEAQEVSSVSTYEHLS
HHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHC
SPLDPRFTFDNFVVGKPNELAFAAARRVAESSEPIPGSNPLFLYGGVGLGKTHLMHAIAW
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
YALGSPRKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDSTQ
HHCCCCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCHHHCCCCCHH
EEFFHTFNALIDQSKQLVISADRSPSDLDGVEDRIKSRLGWGLVADINETTFELRLGILQ
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
LKVEKMGIHVPDEVLEFLAKNIKSNIRELEGALNKVVAHSSLVGSSVTVELASDILSDLL
EEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RANHRLVTIDAIQKKVAEFFGIRLEDMYSARRLRALARPRQVAMYLSKRLTQKSLPDIGK
HCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH
SFGGRDHATVIHAVKQVEKFIDTDSKLAGDISLLIRMLR
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDRDSTYDGHLSWQSVQKKLRDFYGSAAYDSWLSALVYSGYENGRVILLVPTRFIKEWIL
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHH
VHYLEKILEYWRGENSAVYCIDICVADGAESSVLQGNRGVSVRSTEAQEVSSVSTYEHLS
HHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHHHC
SPLDPRFTFDNFVVGKPNELAFAAARRVAESSEPIPGSNPLFLYGGVGLGKTHLMHAIAW
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHH
YALGSPRKRKIAYLSAEKFMYQYVTALRSKDIMLFKEQFRSVDVLMVDDVQFISGKDSTQ
HHCCCCCCHHEEHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCHHHCCCCCHH
EEFFHTFNALIDQSKQLVISADRSPSDLDGVEDRIKSRLGWGLVADINETTFELRLGILQ
HHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
LKVEKMGIHVPDEVLEFLAKNIKSNIRELEGALNKVVAHSSLVGSSVTVELASDILSDLL
EEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
RANHRLVTIDAIQKKVAEFFGIRLEDMYSARRLRALARPRQVAMYLSKRLTQKSLPDIGK
HCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCHHHHH
SFGGRDHATVIHAVKQVEKFIDTDSKLAGDISLLIRMLR
HCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA