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The map label for this gene is def

Identifier: 87200909

GI number: 87200909

Start: 3115464

End: 3116030

Strand: Direct

Name: def

Synonym: Saro_2896

Alternate gene names: 87200909

Gene position: 3115464-3116030 (Clockwise)

Preceding gene: 87200908

Following gene: 87200910

Centisome position: 87.47

GC content: 64.02

Gene sequence:

>567_bases
ATGGCCATCCGCGAAATCATCGAAGTCCCCGACCCGCGCCTGAAGCAGGTCTCCGTCCCCGTCGAGAAGTTCGACGACGA
GCTGAAGACGCTTGTCGAGGACATGTTCGAAACGATGTACGATGCCCCCGGCATCGGCCTCGCCGCGATCCAGGTGGGCG
TGCCCCTGCGCGTGCTGGTGATCGACCTCCAGCCCGACGATCCCGACGCCGAGCCGGTCGCCTGCGACCATGACGGCCAC
CATCACCACCACCAGCCGACGAAGAAGGAGCCGCGGGTCTTCATCAACCCCGAGATCCTCGATCCGTCGGAAGAATACAG
CGTCTACCAGGAAGGCTGTCTCTCGGTCCCCGAGATCTACGCCGAGGTGGAACGCCCCGCCACGATCCGCGCGCGCTGGC
AGGATCTAGACGGCAAGGTCCACGAGGAGCAGATGGAAGGCCTGATGGCCACTTGCCTCCAGCACGAGATGGACCATCTC
GAGGGCGTGCTGTTCATCGACCACCTCTCGCGCCTCAAGCGCAACATGGCGCTCAAGAAGCTGGAAAAGCTGCGCAAGGC
GGCGTGA

Upstream 100 bases:

>100_bases
CGCAGGCCCTGCGGGCAAGGCGCCCGGTGGGCTGAGAGCCGATCACACTGATCGAAACGGTGTCCTTGCGACTCCGCCGG
ACATTCCTTATCTGCGCCCC

Downstream 100 bases:

>100_bases
CGCATCGAGGCCGCGCTACGCGGCCTCGTGCACTTCCTTGTCGAGGTCTTCCAGCGCCCGGCGGCAATCGTCGGCGCATT
CGCGACACATCTGCGCGCAG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MAIREIIEVPDPRLKQVSVPVEKFDDELKTLVEDMFETMYDAPGIGLAAIQVGVPLRVLVIDLQPDDPDAEPVACDHDGH
HHHHQPTKKEPRVFINPEILDPSEEYSVYQEGCLSVPEIYAEVERPATIRARWQDLDGKVHEEQMEGLMATCLQHEMDHL
EGVLFIDHLSRLKRNMALKKLEKLRKAA

Sequences:

>Translated_188_residues
MAIREIIEVPDPRLKQVSVPVEKFDDELKTLVEDMFETMYDAPGIGLAAIQVGVPLRVLVIDLQPDDPDAEPVACDHDGH
HHHHQPTKKEPRVFINPEILDPSEEYSVYQEGCLSVPEIYAEVERPATIRARWQDLDGKVHEEQMEGLMATCLQHEMDHL
EGVLFIDHLSRLKRNMALKKLEKLRKAA
>Mature_187_residues
AIREIIEVPDPRLKQVSVPVEKFDDELKTLVEDMFETMYDAPGIGLAAIQVGVPLRVLVIDLQPDDPDAEPVACDHDGHH
HHHQPTKKEPRVFINPEILDPSEEYSVYQEGCLSVPEIYAEVERPATIRARWQDLDGKVHEEQMEGLMATCLQHEMDHLE
GVLFIDHLSRLKRNMALKKLEKLRKAA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=184, Percent_Identity=32.6086956521739, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1789682, Length=184, Percent_Identity=42.3913043478261, Blast_Score=139, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24645728, Length=186, Percent_Identity=31.7204301075269, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24645726, Length=169, Percent_Identity=31.9526627218935, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_NOVAD (Q2G491)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_498166.1
- ProteinModelPortal:   Q2G491
- SMR:   Q2G491
- STRING:   Q2G491
- GeneID:   3915535
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_2896
- NMPDR:   fig|48935.1.peg.3544
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   ETMIASE
- PhylomeDB:   Q2G491
- ProtClustDB:   PRK00150
- BioCyc:   NARO279238:SARO_2896-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 21485; Mature: 21354

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: ACT_SITE 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIREIIEVPDPRLKQVSVPVEKFDDELKTLVEDMFETMYDAPGIGLAAIQVGVPLRVLV
CCHHHHHHCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEE
IDLQPDDPDAEPVACDHDGHHHHHQPTKKEPRVFINPEILDPSEEYSVYQEGCLSVPEIY
EEECCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHH
AEVERPATIRARWQDLDGKVHEEQMEGLMATCLQHEMDHLEGVLFIDHLSRLKRNMALKK
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKLRKAA
HHHHHHCC
>Mature Secondary Structure 
AIREIIEVPDPRLKQVSVPVEKFDDELKTLVEDMFETMYDAPGIGLAAIQVGVPLRVLV
CHHHHHHCCCCCHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEE
IDLQPDDPDAEPVACDHDGHHHHHQPTKKEPRVFINPEILDPSEEYSVYQEGCLSVPEIY
EEECCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCHHHHH
AEVERPATIRARWQDLDGKVHEEQMEGLMATCLQHEMDHLEGVLFIDHLSRLKRNMALKK
HHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEKLRKAA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA