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The map label for this gene is pdhC [H]

Identifier: 87199963

GI number: 87199963

Start: 2062376

End: 2063659

Strand: Reverse

Name: pdhC [H]

Synonym: Saro_1946

Alternate gene names: 87199963

Gene position: 2063659-2062376 (Counterclockwise)

Preceding gene: 87199965

Following gene: 87199962

Centisome position: 57.94

GC content: 64.8

Gene sequence:

>1284_bases
ATGCCCATCGCCATCAAGATGCCCGCCCTCTCCCCGACGATGGAGGAGGGAACTCTCGCCAAGTGGCTGGTGAAGGTGGG
CGATAAGGTCTCTTCCGGCGACATCATGGCCGAGATCGAGACCGACAAGGCGACCATGGAATTCGAAGCGGTCGATGAAG
GCACGATTGTTTCCATTGACGTGGCCGAAGGATCTGAAGGCGTGAAGGTCGGGACCGTCATCGCAACCTTGGCGGGCGAG
GATGAAGATGCCAGTGCTCCCGCTCCTAAGGCCGTCGCTCCCGCTGCGGCGCCTGTGCCGGTTCCTGCGCCCAAGGCAGA
GCCCGCGCCGGCGGCGGTTTCCACCCCCGCTCCTGCAGCGGCGTCCGCTAGCAAGGGTGACCGCGTAATCGCCACCCCGC
TGGCCAAGCGCATCGCGGCGGACAAGGGTATTGATCTCAAGGGTGTGGCAGGCTCTGGTCCTAATGGGCGCATTATTCGC
GCCGACGTCGAGGGTGCGAAGCCCGCCGCTGCCGCGCCAGTTTCTACCGTGGCGCCCGCGGTCGCGTCGGCAGCCGCCCC
TGCTCGTGCCCCGGCGGCCGTGCCAGACTTCGGCATCCCCTACGAGGCGCAGAAGCTCAACAATGTGCGCAAGACCATCG
CGCGCCGCCTGACCGAGGCGAAGCAGACGATCCCGCACATCTATCTCACCGTCGACATTCGCCTCGACGCGCTGCTCAAG
CTGCGCGGTGATCTGAACAAGGCGCTCGAGGCACAGGGCGTCAAGTTGTCGGTCAACGACCTCATCATCAAGGCGCTGGC
CAAGGCGCTGATGCAGGTGCCCAAGTGCAACGTCAGCTTTGCCGGCGACGAACTGCGCAGCTTCAAGCGCGCGGATATTT
CGGTGGCCGTTGCCGCGCCGTCGGGCCTGATTACGCCGATCATTGTCGATGCCGGCTCGAAGTCTGTCTCCGCCATCGCC
ACCGAGATGAAGGCGCTGGCCAACAAGGCTCGTGAGGGCAAGCTGCAGCCGCACGAGTACCAGGGCGGGACCGCATCGCT
TTCGAACCTCGGCATGTTCGGCATCAAGAACTTCGATGCGGTAATCAACCCGCCGCAGGCGATGATCATGGCTGTCGGCG
CGGGCGAACAGCGCCCCTACGTCATCGACGGTGCGCTTGGCATCGCCACGGTCATGTCGGCGACGGGCAGCTTCGATCAC
CGCGCGATCGACGGAGCGGATGGCGCTGAACTCATGCAGGCGTTCAAGAACCTGATCGAGAACCCGCTCGGCCTGGTCGC
CTGA

Upstream 100 bases:

>100_bases
GACACGCCATGCTGGTGAAATGGCGCCGAGAAAAGATGCAGGTGAGGTTTCCGCTACGGCGCCTTCATCCCTGCGCTACC
AGGACACGGAAAACACGCCC

Downstream 100 bases:

>100_bases
CGCCTGACCATTCTGCGAGCGGCAGCATGCCGCCCGCTTCGCTAGATCATGGGCCGAAAATGGCCTGAAGGAGCATTTGC
GTGGCTGAACAGTATGACGT

Product: dihydrolipoamide acetyltransferase, long form

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MPIAIKMPALSPTMEEGTLAKWLVKVGDKVSSGDIMAEIETDKATMEFEAVDEGTIVSIDVAEGSEGVKVGTVIATLAGE
DEDASAPAPKAVAPAAAPVPVPAPKAEPAPAAVSTPAPAAASASKGDRVIATPLAKRIAADKGIDLKGVAGSGPNGRIIR
ADVEGAKPAAAAPVSTVAPAVASAAAPARAPAAVPDFGIPYEAQKLNNVRKTIARRLTEAKQTIPHIYLTVDIRLDALLK
LRGDLNKALEAQGVKLSVNDLIIKALAKALMQVPKCNVSFAGDELRSFKRADISVAVAAPSGLITPIIVDAGSKSVSAIA
TEMKALANKAREGKLQPHEYQGGTASLSNLGMFGIKNFDAVINPPQAMIMAVGAGEQRPYVIDGALGIATVMSATGSFDH
RAIDGADGAELMQAFKNLIENPLGLVA

Sequences:

>Translated_427_residues
MPIAIKMPALSPTMEEGTLAKWLVKVGDKVSSGDIMAEIETDKATMEFEAVDEGTIVSIDVAEGSEGVKVGTVIATLAGE
DEDASAPAPKAVAPAAAPVPVPAPKAEPAPAAVSTPAPAAASASKGDRVIATPLAKRIAADKGIDLKGVAGSGPNGRIIR
ADVEGAKPAAAAPVSTVAPAVASAAAPARAPAAVPDFGIPYEAQKLNNVRKTIARRLTEAKQTIPHIYLTVDIRLDALLK
LRGDLNKALEAQGVKLSVNDLIIKALAKALMQVPKCNVSFAGDELRSFKRADISVAVAAPSGLITPIIVDAGSKSVSAIA
TEMKALANKAREGKLQPHEYQGGTASLSNLGMFGIKNFDAVINPPQAMIMAVGAGEQRPYVIDGALGIATVMSATGSFDH
RAIDGADGAELMQAFKNLIENPLGLVA
>Mature_426_residues
PIAIKMPALSPTMEEGTLAKWLVKVGDKVSSGDIMAEIETDKATMEFEAVDEGTIVSIDVAEGSEGVKVGTVIATLAGED
EDASAPAPKAVAPAAAPVPVPAPKAEPAPAAVSTPAPAAASASKGDRVIATPLAKRIAADKGIDLKGVAGSGPNGRIIRA
DVEGAKPAAAAPVSTVAPAVASAAAPARAPAAVPDFGIPYEAQKLNNVRKTIARRLTEAKQTIPHIYLTVDIRLDALLKL
RGDLNKALEAQGVKLSVNDLIIKALAKALMQVPKCNVSFAGDELRSFKRADISVAVAAPSGLITPIIVDAGSKSVSAIAT
EMKALANKAREGKLQPHEYQGGTASLSNLGMFGIKNFDAVINPPQAMIMAVGAGEQRPYVIDGALGIATVMSATGSFDHR
AIDGADGAELMQAFKNLIENPLGLVA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=438, Percent_Identity=44.7488584474886, Blast_Score=341, Evalue=7e-94,
Organism=Homo sapiens, GI203098753, Length=454, Percent_Identity=38.9867841409692, Blast_Score=270, Evalue=2e-72,
Organism=Homo sapiens, GI203098816, Length=454, Percent_Identity=38.9867841409692, Blast_Score=269, Evalue=3e-72,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=46.7065868263473, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=27.3563218390805, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI19923748, Length=226, Percent_Identity=31.4159292035398, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=28.8056206088993, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786305, Length=420, Percent_Identity=32.1428571428571, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=43.9732142857143, Blast_Score=317, Evalue=8e-87,
Organism=Caenorhabditis elegans, GI17538894, Length=305, Percent_Identity=37.7049180327869, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=428, Percent_Identity=29.9065420560748, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=438, Percent_Identity=28.310502283105, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=449, Percent_Identity=40.3118040089087, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6320352, Length=425, Percent_Identity=28.7058823529412, Blast_Score=152, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321632, Length=170, Percent_Identity=35.2941176470588, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI20129315, Length=446, Percent_Identity=41.2556053811659, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24582497, Length=435, Percent_Identity=40.6896551724138, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=29.6037296037296, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=31.25, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 43626; Mature: 43495

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIAIKMPALSPTMEEGTLAKWLVKVGDKVSSGDIMAEIETDKATMEFEAVDEGTIVSID
CCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VAEGSEGVKVGTVIATLAGEDEDASAPAPKAVAPAAAPVPVPAPKAEPAPAAVSTPAPAA
ECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASASKGDRVIATPLAKRIAADKGIDLKGVAGSGPNGRIIRADVEGAKPAAAAPVSTVAPA
CCCCCCCEEEEHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
VASAAAPARAPAAVPDFGIPYEAQKLNNVRKTIARRLTEAKQTIPHIYLTVDIRLDALLK
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHH
LRGDLNKALEAQGVKLSVNDLIIKALAKALMQVPKCNVSFAGDELRSFKRADISVAVAAP
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHCCEEEEEECC
SGLITPIIVDAGSKSVSAIATEMKALANKAREGKLQPHEYQGGTASLSNLGMFGIKNFDA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCH
VINPPQAMIMAVGAGEQRPYVIDGALGIATVMSATGSFDHRAIDGADGAELMQAFKNLIE
HCCCCHHEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
NPLGLVA
CCCCCCC
>Mature Secondary Structure 
PIAIKMPALSPTMEEGTLAKWLVKVGDKVSSGDIMAEIETDKATMEFEAVDEGTIVSID
CEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCEEEEE
VAEGSEGVKVGTVIATLAGEDEDASAPAPKAVAPAAAPVPVPAPKAEPAPAAVSTPAPAA
ECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASASKGDRVIATPLAKRIAADKGIDLKGVAGSGPNGRIIRADVEGAKPAAAAPVSTVAPA
CCCCCCCEEEEHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCHHHHHHH
VASAAAPARAPAAVPDFGIPYEAQKLNNVRKTIARRLTEAKQTIPHIYLTVDIRLDALLK
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHH
LRGDLNKALEAQGVKLSVNDLIIKALAKALMQVPKCNVSFAGDELRSFKRADISVAVAAP
HHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHCCEEEEEECC
SGLITPIIVDAGSKSVSAIATEMKALANKAREGKLQPHEYQGGTASLSNLGMFGIKNFDA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCH
VINPPQAMIMAVGAGEQRPYVIDGALGIATVMSATGSFDHRAIDGADGAELMQAFKNLIE
HCCCCHHEEEEECCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
NPLGLVA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]