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The map label for this gene is dacA [H]

Identifier: 87199959

GI number: 87199959

Start: 2057175

End: 2058350

Strand: Reverse

Name: dacA [H]

Synonym: Saro_1942

Alternate gene names: 87199959

Gene position: 2058350-2057175 (Counterclockwise)

Preceding gene: 87199960

Following gene: 87199958

Centisome position: 57.79

GC content: 63.1

Gene sequence:

>1176_bases
TTGCGCCTGAAGTTCGTTGCCGTCGCCGCTGGATTGATTGTGGGCGCCATGCCGGTACAGGCGGCTGCGCCCGAAGTGGG
GCTGGATGCCGCCATGACGTCGCTGCCACGCATCGACGCGCCCATAGCCCTTCTCGTTGACTCCGGATCGGGACGGACGC
TTTATGCGCGAGACGTACACAGGCGTTTCGTGCCGGCTTCGATCACCAAGATCATGACCAGCTATGTCGCGTTCGATCTT
GTTCGAAAGGGCAAGCTGCGGCTCGATCAACGTATTTCGATCCGGCCGGAGACATTTCGCAAGTGGAAGAACGTGGGCAG
TACGATGTTCCTGCCCGCAGAAGCGCAACCGACGGTCGCAGAACTGATCGAGGGCATTGTCACCGTTTCGGCCAACGATG
CCTGTGTGGTGCTGGCCGAAGGCGCGGTCGGATCAGTACCCGCGTTCACCCGGCTGATGAACGAGGCGGCGCTGCAGCTC
GGCATGCGCGACAGTCACTTCAACACGCCGAACGGGTGGATGGACGAAGGCCAGACCTATGTGACGGCAGACGATCTCGC
GAAGCTGAGCGGCGCGCTGATGACGCGCTTTCCGGACCTGTACAAGCGGTTTTACGGCCACGCCACGATGACCTGGAATG
GCATTACCCAGCCTAACCACAACCCCTTGTATGGGCATACGGAGGGCGCAGACGGAGTGAAGACCGGCTTCACCAACGAG
GCCGGCTATGGCTTCGTCGGTTCTGCGGAGCGCGGTGGACGCAGGCTGATCATGGTGCTGGGGGGGTATGATCGTCCGAT
CGACCGGACGAACCAGTCGCGCGCGCTGATCGAATGGGGCTTTTCCGCGTGGGAGTCGCATCCGTTGTTCAAGGCGGGCG
CTCCTGTGGGCGTTGCCGAAGTACAGGGTGGGGCAGAAAGGCAGGTCTCGCTCGTTGCGCCACGTGCGCTAGGCGTGACG
ACACCACAAGGGCAGAAGCCGCGCTACACGTTGTCGATCCGCTACAAAGGCCCGCTCAGGGCTCCGATCGCCAAGGGGGA
GACGGTGGCGAGCCTTTTGGTGCGGGTGCCGGGAGAGCCAGTCCACGCATTGCCCCTCGTCGCAGCCAATGCGGTGCCGA
AGGGCGGGGTGGCTTCGCGACTGCGCAATGGGTTACTGGGGCTTGTCGGGCTGTGA

Upstream 100 bases:

>100_bases
GGCGCTGGCGAAGGTGCGCGCGGCGGGCTATGCCGACGCGAGGGTCGTCGCTGCTCCTTGAGCCCTGGTGCGGCGGCTCC
GTGTGACCGGGGAATGCCTT

Downstream 100 bases:

>100_bases
GCCGGGGACGTTTTGTTGCCTTCGAGGGGGGCGAGGGCGTCGGCAAGTCGACGCAGGGACGGCTTCTGGCGGAGTCGCTG
CGTGCGCGAGGGCTGGAAGT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRLKFVAVAAGLIVGAMPVQAAAPEVGLDAAMTSLPRIDAPIALLVDSGSGRTLYARDVHRRFVPASITKIMTSYVAFDL
VRKGKLRLDQRISIRPETFRKWKNVGSTMFLPAEAQPTVAELIEGIVTVSANDACVVLAEGAVGSVPAFTRLMNEAALQL
GMRDSHFNTPNGWMDEGQTYVTADDLAKLSGALMTRFPDLYKRFYGHATMTWNGITQPNHNPLYGHTEGADGVKTGFTNE
AGYGFVGSAERGGRRLIMVLGGYDRPIDRTNQSRALIEWGFSAWESHPLFKAGAPVGVAEVQGGAERQVSLVAPRALGVT
TPQGQKPRYTLSIRYKGPLRAPIAKGETVASLLVRVPGEPVHALPLVAANAVPKGGVASRLRNGLLGLVGL

Sequences:

>Translated_391_residues
MRLKFVAVAAGLIVGAMPVQAAAPEVGLDAAMTSLPRIDAPIALLVDSGSGRTLYARDVHRRFVPASITKIMTSYVAFDL
VRKGKLRLDQRISIRPETFRKWKNVGSTMFLPAEAQPTVAELIEGIVTVSANDACVVLAEGAVGSVPAFTRLMNEAALQL
GMRDSHFNTPNGWMDEGQTYVTADDLAKLSGALMTRFPDLYKRFYGHATMTWNGITQPNHNPLYGHTEGADGVKTGFTNE
AGYGFVGSAERGGRRLIMVLGGYDRPIDRTNQSRALIEWGFSAWESHPLFKAGAPVGVAEVQGGAERQVSLVAPRALGVT
TPQGQKPRYTLSIRYKGPLRAPIAKGETVASLLVRVPGEPVHALPLVAANAVPKGGVASRLRNGLLGLVGL
>Mature_391_residues
MRLKFVAVAAGLIVGAMPVQAAAPEVGLDAAMTSLPRIDAPIALLVDSGSGRTLYARDVHRRFVPASITKIMTSYVAFDL
VRKGKLRLDQRISIRPETFRKWKNVGSTMFLPAEAQPTVAELIEGIVTVSANDACVVLAEGAVGSVPAFTRLMNEAALQL
GMRDSHFNTPNGWMDEGQTYVTADDLAKLSGALMTRFPDLYKRFYGHATMTWNGITQPNHNPLYGHTEGADGVKTGFTNE
AGYGFVGSAERGGRRLIMVLGGYDRPIDRTNQSRALIEWGFSAWESHPLFKAGAPVGVAEVQGGAERQVSLVAPRALGVT
TPQGQKPRYTLSIRYKGPLRAPIAKGETVASLLVRVPGEPVHALPLVAANAVPKGGVASRLRNGLLGLVGL

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Note=N-terminal lies in the periplasmic space (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=369, Percent_Identity=33.3333333333333, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1787062, Length=386, Percent_Identity=33.419689119171, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI87082022, Length=356, Percent_Identity=32.8651685393258, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41837; Mature: 41837

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKFVAVAAGLIVGAMPVQAAAPEVGLDAAMTSLPRIDAPIALLVDSGSGRTLYARDVH
CCEEHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHH
RRFVPASITKIMTSYVAFDLVRKGKLRLDQRISIRPETFRKWKNVGSTMFLPAEAQPTVA
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHCCCCEEEECCCCCCHHH
ELIEGIVTVSANDACVVLAEGAVGSVPAFTRLMNEAALQLGMRDSHFNTPNGWMDEGQTY
HHHHHHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
VTADDLAKLSGALMTRFPDLYKRFYGHATMTWNGITQPNHNPLYGHTEGADGVKTGFTNE
EEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGYGFVGSAERGGRRLIMVLGGYDRPIDRTNQSRALIEWGFSAWESHPLFKAGAPVGVAE
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHCCCCCEECCCCCCHHH
VQGGAERQVSLVAPRALGVTTPQGQKPRYTLSIRYKGPLRAPIAKGETVASLLVRVPGEP
CCCCCCCEEEEECCHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCC
VHALPLVAANAVPKGGVASRLRNGLLGLVGL
CHHHHHHHHCCCCCCHHHHHHHCCCEEECCC
>Mature Secondary Structure
MRLKFVAVAAGLIVGAMPVQAAAPEVGLDAAMTSLPRIDAPIALLVDSGSGRTLYARDVH
CCEEHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHH
RRFVPASITKIMTSYVAFDLVRKGKLRLDQRISIRPETFRKWKNVGSTMFLPAEAQPTVA
HHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHCCCCEEEECCCCCCHHH
ELIEGIVTVSANDACVVLAEGAVGSVPAFTRLMNEAALQLGMRDSHFNTPNGWMDEGQTY
HHHHHHEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEE
VTADDLAKLSGALMTRFPDLYKRFYGHATMTWNGITQPNHNPLYGHTEGADGVKTGFTNE
EEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
AGYGFVGSAERGGRRLIMVLGGYDRPIDRTNQSRALIEWGFSAWESHPLFKAGAPVGVAE
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCHHHHCCCCCEECCCCCCHHH
VQGGAERQVSLVAPRALGVTTPQGQKPRYTLSIRYKGPLRAPIAKGETVASLLVRVPGEP
CCCCCCCEEEEECCHHCCCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHCCCCC
VHALPLVAANAVPKGGVASRLRNGLLGLVGL
CHHHHHHHHCCCCCCHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]