BacMap

Definition	Novosphingobium aromaticivorans DSM 12444 chromosome, complete genome.
Accession	NC_007794
Length	3,561,584

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The map label for this gene is rhlG [H]

Identifier: 87199023

GI number: 87199023

Start: 1045340

End: 1046143

Strand: Direct

Name: rhlG [H]

Synonym: Saro_1001

Alternate gene names: 87199023

Gene position: 1045340-1046143 (Clockwise)

Preceding gene: 87199022

Following gene: 87199024

Centisome position: 29.35

GC content: 63.56

Gene sequence:

>804_bases
ATGGATTTCAGCAAGCTTTTCAGCCTTGAAGGCCGCGTAGCGGTCGTCACCGGTGGTTCGCGAGGGATCGGCGAGATGAT
TGCCGAGGGTTTCATCGCCGCCGGTTGCTCGCGCGTCTACATCACCGCGCGCAAAGCCGCCGTGGTCGAGGAAACCGCTG
CCCGCCTTGGCGAGAAGTGCATCGCCCTGCCAGGTGACATCTCCAACGTCGCCGGGATCGAACAGCTCGCCGCCGACCTC
GCCGCGCGCGAGGACAAGCTCGACATTCTCGTCAACAACGCCGGCGCGGCGTGGGGCGCGGATTTCGAGGATTTTCCTGA
ACTGGGCTGGGACAAGGTCATGGACCTCAACGTCAAGACCCCGTTCTTCCTCACCCAGAAGCTGCATGGCCTGCTCAAGG
CCGCTGCCAGCAAGGAACAGCCCGCCAAGGTCATCAACATCGCCTCGGTGGATGGCCTGCGCCTCAACCCCTGGGAAACC
TACAGCTACCAGGCTTCGAAGGCGGCGGTCGTTCATCTGACGAAGCGAATGGCGGCACGCCTGGTCAAGGACCAGATCAT
TGCGAGCTGCATCTGCCCCGGCGCCTTCCCCTCGGAAATGAACAAGGCGGCAGCGAAGAATCCGGAAGCCTCGTCGCGTG
GCATTCCGCTGAAGCGTATCGGCACGATCGAGGACATGGCCGGCGGCGCGATCTTCCTTGCCAGTCGCGCGGGCGACTAT
GTCGTCGGCACGCCGCTTCCCATCGACGGCGGCATCGTCAACGCGTGGATTCCGGAAAACGCCGTCGATCCGAGCGGACA
CTGA

Upstream 100 bases:

>100_bases
GTCGGGATGTGTCGGCGGCGGGACAGTACCGCTGTCCGTCCCGAACCTGTCGAAGGACCAGGGTACAACGGCCGCAAAGC
AGGTCGGAAGGGAACACGAT

Downstream 100 bases:

>100_bases
CGCCGATGCCGCACACCGTTCGCACGCTGGCGGAAGGCCTGACCTTCGGTGAAGGCCCTCGCTGGCGGGCGGACGATCGC
GGTGGCCGGCTCTGGTTCTC

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 3-ketoacyl-acyl carrier protein reductase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MDFSKLFSLEGRVAVVTGGSRGIGEMIAEGFIAAGCSRVYITARKAAVVEETAARLGEKCIALPGDISNVAGIEQLAADL
AAREDKLDILVNNAGAAWGADFEDFPELGWDKVMDLNVKTPFFLTQKLHGLLKAAASKEQPAKVINIASVDGLRLNPWET
YSYQASKAAVVHLTKRMAARLVKDQIIASCICPGAFPSEMNKAAAKNPEASSRGIPLKRIGTIEDMAGGAIFLASRAGDY
VVGTPLPIDGGIVNAWIPENAVDPSGH

Sequences:

>Translated_267_residues
MDFSKLFSLEGRVAVVTGGSRGIGEMIAEGFIAAGCSRVYITARKAAVVEETAARLGEKCIALPGDISNVAGIEQLAADL
AAREDKLDILVNNAGAAWGADFEDFPELGWDKVMDLNVKTPFFLTQKLHGLLKAAASKEQPAKVINIASVDGLRLNPWET
YSYQASKAAVVHLTKRMAARLVKDQIIASCICPGAFPSEMNKAAAKNPEASSRGIPLKRIGTIEDMAGGAIFLASRAGDY
VVGTPLPIDGGIVNAWIPENAVDPSGH
>Mature_267_residues
MDFSKLFSLEGRVAVVTGGSRGIGEMIAEGFIAAGCSRVYITARKAAVVEETAARLGEKCIALPGDISNVAGIEQLAADL
AAREDKLDILVNNAGAAWGADFEDFPELGWDKVMDLNVKTPFFLTQKLHGLLKAAASKEQPAKVINIASVDGLRLNPWET
YSYQASKAAVVHLTKRMAARLVKDQIIASCICPGAFPSEMNKAAAKNPEASSRGIPLKRIGTIEDMAGGAIFLASRAGDY
VVGTPLPIDGGIVNAWIPENAVDPSGH

Specific function: Required for the synthesis of the beta-hydroxy acid moiety of rhamnolipids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=259, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI32483357, Length=250, Percent_Identity=28, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI66933014, Length=194, Percent_Identity=33.5051546391753, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI5031737, Length=251, Percent_Identity=30.2788844621514, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI109715829, Length=243, Percent_Identity=29.2181069958848, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI10190704, Length=258, Percent_Identity=28.2945736434109, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI15277342, Length=256, Percent_Identity=28.515625, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI7705925, Length=261, Percent_Identity=26.8199233716475, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI33667109, Length=200, Percent_Identity=30, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI142976729, Length=222, Percent_Identity=29.2792792792793, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI126723750, Length=258, Percent_Identity=26.3565891472868, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789208, Length=252, Percent_Identity=36.9047619047619, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790717, Length=258, Percent_Identity=31.3953488372093, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI87082160, Length=258, Percent_Identity=31.0077519379845, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI87082100, Length=264, Percent_Identity=31.0606060606061, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787335, Length=247, Percent_Identity=28.7449392712551, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787905, Length=253, Percent_Identity=32.0158102766798, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788459, Length=178, Percent_Identity=33.1460674157303, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI2367175, Length=248, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789378, Length=247, Percent_Identity=27.5303643724696, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367365, Length=243, Percent_Identity=24.6913580246914, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1786812, Length=262, Percent_Identity=24.8091603053435, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787820, Length=193, Percent_Identity=31.0880829015544, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=246, Percent_Identity=30.0813008130081, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25147288, Length=247, Percent_Identity=28.7449392712551, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=30.859375, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17562906, Length=269, Percent_Identity=29.7397769516729, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559104, Length=263, Percent_Identity=30.4182509505703, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17560676, Length=253, Percent_Identity=30.8300395256917, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17531453, Length=269, Percent_Identity=29.368029739777, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562908, Length=272, Percent_Identity=29.0441176470588, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193204405, Length=270, Percent_Identity=29.2592592592593, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17551412, Length=262, Percent_Identity=29.3893129770992, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=255, Percent_Identity=25.0980392156863, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=29.1666666666667, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17538480, Length=266, Percent_Identity=27.0676691729323, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=262, Percent_Identity=30.1526717557252, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=263, Percent_Identity=28.5171102661597, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17560220, Length=269, Percent_Identity=28.996282527881, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=26.7175572519084, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=265, Percent_Identity=27.9245283018868, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=258, Percent_Identity=27.5193798449612, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=259, Percent_Identity=26.6409266409266, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=266, Percent_Identity=26.3157894736842, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17565030, Length=261, Percent_Identity=28.3524904214559, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17544670, Length=272, Percent_Identity=26.1029411764706, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=264, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=259, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=254, Percent_Identity=25.1968503937008, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355319, Length=254, Percent_Identity=32.6771653543307, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28571526, Length=257, Percent_Identity=27.6264591439689, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357041, Length=255, Percent_Identity=27.0588235294118, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24644339, Length=256, Percent_Identity=26.953125, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24639444, Length=250, Percent_Identity=28.8, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24643142, Length=246, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=28.6274509803922, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24644337, Length=261, Percent_Identity=29.5019157088123, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45553409, Length=212, Percent_Identity=29.7169811320755, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24647948, Length=212, Percent_Identity=29.7169811320755, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24647946, Length=212, Percent_Identity=29.7169811320755, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21358495, Length=200, Percent_Identity=28.5, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.100 [H]

Molecular weight: Translated: 28193; Mature: 28193

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSKLFSLEGRVAVVTGGSRGIGEMIAEGFIAAGCSRVYITARKAAVVEETAARLGEKC
CCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCHHE
IALPGDISNVAGIEQLAADLAAREDKLDILVNNAGAAWGADFEDFPELGWDKVMDLNVKT
EECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEEECCCCC
PFFLTQKLHGLLKAAASKEQPAKVINIASVDGLRLNPWETYSYQASKAAVVHLTKRMAAR
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHH
LVKDQIIASCICPGAFPSEMNKAAAKNPEASSRGIPLKRIGTIEDMAGGAIFLASRAGDY
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHCCCEEEEEECCCCE
VVGTPLPIDGGIVNAWIPENAVDPSGH
EECCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MDFSKLFSLEGRVAVVTGGSRGIGEMIAEGFIAAGCSRVYITARKAAVVEETAARLGEKC
CCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHCHHE
IALPGDISNVAGIEQLAADLAAREDKLDILVNNAGAAWGADFEDFPELGWDKVMDLNVKT
EECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCCCEEECCCCC
PFFLTQKLHGLLKAAASKEQPAKVINIASVDGLRLNPWETYSYQASKAAVVHLTKRMAAR
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHH
LVKDQIIASCICPGAFPSEMNKAAAKNPEASSRGIPLKRIGTIEDMAGGAIFLASRAGDY
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHCCCHHHHCCCEEEEEECCCCE
VVGTPLPIDGGIVNAWIPENAVDPSGH
EECCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9721281; 10984043 [H]