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The map label for this gene is moaA [H]

Identifier: 87198947

GI number: 87198947

Start: 976560

End: 977579

Strand: Direct

Name: moaA [H]

Synonym: Saro_0925

Alternate gene names: 87198947

Gene position: 976560-977579 (Clockwise)

Preceding gene: 87198946

Following gene: 87198948

Centisome position: 27.42

GC content: 68.53

Gene sequence:

>1020_bases
GTGAACGCTTCCCCGCCTTCTGCCGACGCGCCCCTGATCGACCGGTTCGCCCGCCGGATCACCTATCTCCGCCTGTCGGT
GACGGATCGCTGCGACCTGCGCTGCGCGTACTGCATGCCCGAGAGGATGGAATTCCTGCCCAAGGCCGAAGTGCTGAGCC
TCGAGGAACTGCACCGCCTCTCGCTCCATTTCATCGCGCGAGGCGTGCGCAAGATCCGCCTGACCGGCGGCGAACCGCTG
GTGCGGCGCGACATGGTGGACCTCGTGCGCGCGCTGGGTCGCAAGCTCGGGGACGGGCTCGACGAACTGACCATGACCAC
CAACGGCACCCGCCTGGCGGAGTTTGCCGGGGACCTGGCGGCGGCGGGGCTTCGGCGGATCAACGTCTCGCTCGACACGC
TCGACCGCGAGGCTTTCGCCAGGCTTTCCCGCCGCGACGTTCTGCCGCAGGTGCTCGAAGGCATCGCCGCCGCACGCGAG
GCCGGCCTGCGGGTCAAGATCAATGCCGTGGCGCTCAAGGGCATCAACGAGGACGAGATTCCCTCACTGGTCGAGTGGGC
GCATGGCCAGGGACATGACATGACCCTGATCGAGGTAATGCCGCTGGGCGAGGTCGAGGGCGATCGCTTCGACCACTACC
TTCCGCTCGACGCCGTGCGCCGCAATCTCGAGAGCCGGTGGACGCTGACGGCGAGCGATGCCCGCAGCGGCGGTCCGGCC
CGCTATGTCGACATTGCCGAGACCGGTGGGCGGCTGGGGTTCATCACCCCGCTTACCGGCAACTTCTGCGACGGCTGCAA
CAGGGTGCGGGTGACGGCCACGGGGCAACTCTACATGTGCCTTGGTGGCGAGGGGCGCGTGGACCTGCGCGCGGCGCTTC
GTTCCGACGACCCTGACGGCGCGCTTGCCGAATGCTTCGCGCGGGCGATGGGCGAAAAGCAGGAGCGCCATTCCTTCGTC
ATCGACAGGCCGGGCGGCGTCCCAGCCGTGTCTCGCCACATGTCGACGACGGGCGGCTGA

Upstream 100 bases:

>100_bases
ACTTCCTCGCATCGGCCAGGTCGGTGACGGGGCAGACTTTGTTCGTCGATGCCGGCGCGAGCCTTGAATCCTACCCCCGT
GACTTCGTATACTTGGAGAA

Downstream 100 bases:

>100_bases
TGGCGAGGCTGGTCTTCATGGGGCGGCTGGAAGACGTCGCGGGGACGGGCGAACTCGCGGTGGAGGCAGGCCCGGTCGAA
CGGATACTGGCGGCGCTTGA

Product: GTP cyclohydrolase subunit MoaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNASPPSADAPLIDRFARRITYLRLSVTDRCDLRCAYCMPERMEFLPKAEVLSLEELHRLSLHFIARGVRKIRLTGGEPL
VRRDMVDLVRALGRKLGDGLDELTMTTNGTRLAEFAGDLAAAGLRRINVSLDTLDREAFARLSRRDVLPQVLEGIAAARE
AGLRVKINAVALKGINEDEIPSLVEWAHGQGHDMTLIEVMPLGEVEGDRFDHYLPLDAVRRNLESRWTLTASDARSGGPA
RYVDIAETGGRLGFITPLTGNFCDGCNRVRVTATGQLYMCLGGEGRVDLRAALRSDDPDGALAECFARAMGEKQERHSFV
IDRPGGVPAVSRHMSTTGG

Sequences:

>Translated_339_residues
MNASPPSADAPLIDRFARRITYLRLSVTDRCDLRCAYCMPERMEFLPKAEVLSLEELHRLSLHFIARGVRKIRLTGGEPL
VRRDMVDLVRALGRKLGDGLDELTMTTNGTRLAEFAGDLAAAGLRRINVSLDTLDREAFARLSRRDVLPQVLEGIAAARE
AGLRVKINAVALKGINEDEIPSLVEWAHGQGHDMTLIEVMPLGEVEGDRFDHYLPLDAVRRNLESRWTLTASDARSGGPA
RYVDIAETGGRLGFITPLTGNFCDGCNRVRVTATGQLYMCLGGEGRVDLRAALRSDDPDGALAECFARAMGEKQERHSFV
IDRPGGVPAVSRHMSTTGG
>Mature_339_residues
MNASPPSADAPLIDRFARRITYLRLSVTDRCDLRCAYCMPERMEFLPKAEVLSLEELHRLSLHFIARGVRKIRLTGGEPL
VRRDMVDLVRALGRKLGDGLDELTMTTNGTRLAEFAGDLAAAGLRRINVSLDTLDREAFARLSRRDVLPQVLEGIAAARE
AGLRVKINAVALKGINEDEIPSLVEWAHGQGHDMTLIEVMPLGEVEGDRFDHYLPLDAVRRNLESRWTLTASDARSGGPA
RYVDIAETGGRLGFITPLTGNFCDGCNRVRVTATGQLYMCLGGEGRVDLRAALRSDDPDGALAECFARAMGEKQERHSFV
IDRPGGVPAVSRHMSTTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=340, Percent_Identity=38.5294117647059, Blast_Score=209, Evalue=4e-54,
Organism=Homo sapiens, GI22027536, Length=340, Percent_Identity=38.5294117647059, Blast_Score=208, Evalue=6e-54,
Organism=Escherichia coli, GI1786999, Length=333, Percent_Identity=35.1351351351351, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32566331, Length=331, Percent_Identity=38.9728096676737, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17561210, Length=311, Percent_Identity=40.5144694533762, Blast_Score=225, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI32566329, Length=311, Percent_Identity=39.871382636656, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI28574490, Length=318, Percent_Identity=38.9937106918239, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28574488, Length=322, Percent_Identity=38.5093167701863, Blast_Score=205, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 37206; Mature: 37206

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASPPSADAPLIDRFARRITYLRLSVTDRCDLRCAYCMPERMEFLPKAEVLSLEELHRL
CCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCEEEECCCHHHHHCCCHHHHCHHHHHHH
SLHFIARGVRKIRLTGGEPLVRRDMVDLVRALGRKLGDGLDELTMTTNGTRLAEFAGDLA
HHHHHHHCHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHH
AAGLRRINVSLDTLDREAFARLSRRDVLPQVLEGIAAAREAGLRVKINAVALKGINEDEI
HHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHC
PSLVEWAHGQGHDMTLIEVMPLGEVEGDRFDHYLPLDAVRRNLESRWTLTASDARSGGPA
HHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHCEEEEECCCCCCCCE
RYVDIAETGGRLGFITPLTGNFCDGCNRVRVTATGQLYMCLGGEGRVDLRAALRSDDPDG
EEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEHHHHHCCCCCCC
ALAECFARAMGEKQERHSFVIDRPGGVPAVSRHMSTTGG
HHHHHHHHHHCCHHHHCCEEEECCCCCCHHHHHHCCCCC
>Mature Secondary Structure
MNASPPSADAPLIDRFARRITYLRLSVTDRCDLRCAYCMPERMEFLPKAEVLSLEELHRL
CCCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCEEEECCCHHHHHCCCHHHHCHHHHHHH
SLHFIARGVRKIRLTGGEPLVRRDMVDLVRALGRKLGDGLDELTMTTNGTRLAEFAGDLA
HHHHHHHCHHEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHH
AAGLRRINVSLDTLDREAFARLSRRDVLPQVLEGIAAAREAGLRVKINAVALKGINEDEI
HHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCHHHC
PSLVEWAHGQGHDMTLIEVMPLGEVEGDRFDHYLPLDAVRRNLESRWTLTASDARSGGPA
HHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHCHHHCEEEEECCCCCCCCE
RYVDIAETGGRLGFITPLTGNFCDGCNRVRVTATGQLYMCLGGEGRVDLRAALRSDDPDG
EEEEEECCCCCEEEECCCCCCCCCCCCEEEEEECCEEEEEECCCCCEEHHHHHCCCCCCC
ALAECFARAMGEKQERHSFVIDRPGGVPAVSRHMSTTGG
HHHHHHHHHHCCHHHHCCEEEECCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA