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The map label for this gene is ydaD [H]

Identifier: 87198946

GI number: 87198946

Start: 975805

End: 976563

Strand: Direct

Name: ydaD [H]

Synonym: Saro_0924

Alternate gene names: 87198946

Gene position: 975805-976563 (Clockwise)

Preceding gene: 87198945

Following gene: 87198947

Centisome position: 27.4

GC content: 67.33

Gene sequence:

>759_bases
ATGACGCGCCCGCTCGCGCTGGTCACGGGCGGCTGGCGCCGGATTGGCGGCGCGATCGCTCGCAAGCTGGCGAGCGAGGG
CTGGGACCTTGCCCTTCACGCCCACCACGACCGTTCGTTCGATGCCGAGTTCAAGGCTCAGCTCGAATGGCTGGGGGCGA
CGGTCTTTCCGGTATCGGGCGATCTCGACGACAGGGACTTTCCGGCGCGCCTGCTGGCCGAAGTCGTCGGCGCCGCGGGC
CGATCGCCGGAGCTGCTGGTCAACTCGGCCTCGCTGTTCCACGACGACCGGGTAGAGACGATCACCCCGGAAGAGCTTGA
GCAGCATTTCCGCGTGAACCTCTTCGCGCCGCTGCTGTTGACGCGCGCTTTCGCCGAGGCGCTGGGTGACCGTGACGGTT
CGGTCGTCAACATCCTCGACCAGCGCGTGCTCAACCCCGTGCCCGACCAGATCAGCTACACGATCTCCAAGCAGGCGCTT
CACGCCTCGGTGCGCACGCTGGCCCGCGCGATGGCGCCCAGGGTCCGCGTGAACGGCGTTGCGCCGGGCCTGATCCTGCC
CACTGCGGACTACGATGCCGAACAATGGCGCCGGCTGGAAGAGATCATGCCGCTGCGGCGCTTGCCCGGCGCGGACGAGA
TTGCCGACGCGGTGCACTTCCTCGCATCGGCCAGGTCGGTGACGGGGCAGACTTTGTTCGTCGATGCCGGCGCGAGCCTT
GAATCCTACCCCCGTGACTTCGTATACTTGGAGAAGTGA

Upstream 100 bases:

>100_bases
ATGACAGGGTGATGTGGGTGGAAGTGAAGATCGTGAAGCTGGCCATCAGCGAGAACGGCGAGGAAATCGGCATTACCCTT
AAGCGGGAGCGCCGGACCTG

Downstream 100 bases:

>100_bases
ACGCTTCCCCGCCTTCTGCCGACGCGCCCCTGATCGACCGGTTCGCCCGCCGGATCACCTATCTCCGCCTGTCGGTGACG
GATCGCTGCGACCTGCGCTG

Product: short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: GSP39 [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTRPLALVTGGWRRIGGAIARKLASEGWDLALHAHHDRSFDAEFKAQLEWLGATVFPVSGDLDDRDFPARLLAEVVGAAG
RSPELLVNSASLFHDDRVETITPEELEQHFRVNLFAPLLLTRAFAEALGDRDGSVVNILDQRVLNPVPDQISYTISKQAL
HASVRTLARAMAPRVRVNGVAPGLILPTADYDAEQWRRLEEIMPLRRLPGADEIADAVHFLASARSVTGQTLFVDAGASL
ESYPRDFVYLEK

Sequences:

>Translated_252_residues
MTRPLALVTGGWRRIGGAIARKLASEGWDLALHAHHDRSFDAEFKAQLEWLGATVFPVSGDLDDRDFPARLLAEVVGAAG
RSPELLVNSASLFHDDRVETITPEELEQHFRVNLFAPLLLTRAFAEALGDRDGSVVNILDQRVLNPVPDQISYTISKQAL
HASVRTLARAMAPRVRVNGVAPGLILPTADYDAEQWRRLEEIMPLRRLPGADEIADAVHFLASARSVTGQTLFVDAGASL
ESYPRDFVYLEK
>Mature_251_residues
TRPLALVTGGWRRIGGAIARKLASEGWDLALHAHHDRSFDAEFKAQLEWLGATVFPVSGDLDDRDFPARLLAEVVGAAGR
SPELLVNSASLFHDDRVETITPEELEQHFRVNLFAPLLLTRAFAEALGDRDGSVVNILDQRVLNPVPDQISYTISKQALH
ASVRTLARAMAPRVRVNGVAPGLILPTADYDAEQWRRLEEIMPLRRLPGADEIADAVHFLASARSVTGQTLFVDAGASLE
SYPRDFVYLEK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=248, Percent_Identity=32.258064516129, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI40254992, Length=239, Percent_Identity=30.1255230125523, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI5031737, Length=242, Percent_Identity=30.9917355371901, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI15277342, Length=250, Percent_Identity=30, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI126723750, Length=252, Percent_Identity=28.968253968254, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI33667109, Length=185, Percent_Identity=32.972972972973, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787335, Length=243, Percent_Identity=32.5102880658436, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787891, Length=243, Percent_Identity=26.7489711934156, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI2367175, Length=243, Percent_Identity=27.1604938271605, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788459, Length=240, Percent_Identity=28.3333333333333, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789378, Length=243, Percent_Identity=29.2181069958848, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=245, Percent_Identity=30.2040816326531, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17555706, Length=251, Percent_Identity=31.0756972111554, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71994604, Length=256, Percent_Identity=30.46875, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17544670, Length=263, Percent_Identity=26.615969581749, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI25147288, Length=247, Percent_Identity=30.3643724696356, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=257, Percent_Identity=28.4046692607004, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=277, Percent_Identity=25.9927797833935, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508651, Length=252, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI23397609, Length=253, Percent_Identity=30.0395256916996, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24644339, Length=245, Percent_Identity=28.9795918367347, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571526, Length=242, Percent_Identity=26.4462809917355, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357041, Length=242, Percent_Identity=26.4462809917355, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 27785; Mature: 27654

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPLALVTGGWRRIGGAIARKLASEGWDLALHAHHDRSFDAEFKAQLEWLGATVFPVSG
CCCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCEEEEECCC
DLDDRDFPARLLAEVVGAAGRSPELLVNSASLFHDDRVETITPEELEQHFRVNLFAPLLL
CCCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
TRAFAEALGDRDGSVVNILDQRVLNPVPDQISYTISKQALHASVRTLARAMAPRVRVNGV
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
APGLILPTADYDAEQWRRLEEIMPLRRLPGADEIADAVHFLASARSVTGQTLFVDAGASL
CCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
ESYPRDFVYLEK
HHCCCCEEEECC
>Mature Secondary Structure 
TRPLALVTGGWRRIGGAIARKLASEGWDLALHAHHDRSFDAEFKAQLEWLGATVFPVSG
CCCEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCEEEEECCC
DLDDRDFPARLLAEVVGAAGRSPELLVNSASLFHDDRVETITPEELEQHFRVNLFAPLLL
CCCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
TRAFAEALGDRDGSVVNILDQRVLNPVPDQISYTISKQALHASVRTLARAMAPRVRVNGV
HHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
APGLILPTADYDAEQWRRLEEIMPLRRLPGADEIADAVHFLASARSVTGQTLFVDAGASL
CCCEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCH
ESYPRDFVYLEK
HHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9298659 [H]