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The map label for this gene is nuoE2 [H]

Identifier: 87198756

GI number: 87198756

Start: 774648

End: 775061

Strand: Direct

Name: nuoE2 [H]

Synonym: Saro_0732

Alternate gene names: 87198756

Gene position: 774648-775061 (Clockwise)

Preceding gene: 87198740

Following gene: 87198757

Centisome position: 21.75

GC content: 66.18

Gene sequence:

>414_bases
ATGGAGAGGTTGGAACAGATCATCGCGTCCCACGCAGGCCGCCAAGGCGCATTGCTGCCGATCCTGCACGATGTGCAGGC
GGCGTTCGGGCACGTATCGGAAGATGCGATCCGCGCGGTTGCGTCCGCGCTGAACCTGAGTCGCGCGGATGTGTTCGGCG
TGGTCAGCTTCTACCATGATTTCAGGCAGTCGCCCGAGCCGCGCGCCGTGCTCAAGCTCTGCCGCGCCGAAGCCTGCCAG
GCGCGTGGCGTCGAAGCGCTGGCCGCGCAATTGCCAGACAATCCCCACGTCAAGATTGAGGCCGTCTACTGTCTCGGGCT
GTGCTCGGTGGGGCCGAACGCGATGGCGGAGGGCAAGGTCCACGCGCGGCTGGATGCGTCAAAGCTGGGCGCGCTGGTGG
AGTCGCTGGCATGA

Upstream 100 bases:

>100_bases
TGGCACTCTCGGGCAGACTGGTTCACACACGGTGGCAGGAGTGAAACATCTCCCATTTGTATGTGTTGCTTACAAAACCG
CTTGGGAGTATCCTGCGCCA

Downstream 100 bases:

>100_bases
CTGTTGTCCGAATTTCCGATGATGCGCTGGCGCTCGCCTGCGGGGCCGACGAAGTCGCGCAAGCCTTTGTCGCCGCGGGC
TGCACGGTGGAGCGTGTCTC

Product: NADH dehydrogenase (ubiquinone), 24 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MERLEQIIASHAGRQGALLPILHDVQAAFGHVSEDAIRAVASALNLSRADVFGVVSFYHDFRQSPEPRAVLKLCRAEACQ
ARGVEALAAQLPDNPHVKIEAVYCLGLCSVGPNAMAEGKVHARLDASKLGALVESLA

Sequences:

>Translated_137_residues
MERLEQIIASHAGRQGALLPILHDVQAAFGHVSEDAIRAVASALNLSRADVFGVVSFYHDFRQSPEPRAVLKLCRAEACQ
ARGVEALAAQLPDNPHVKIEAVYCLGLCSVGPNAMAEGKVHARLDASKLGALVESLA
>Mature_137_residues
MERLEQIIASHAGRQGALLPILHDVQAAFGHVSEDAIRAVASALNLSRADVFGVVSFYHDFRQSPEPRAVLKLCRAEACQ
ARGVEALAAQLPDNPHVKIEAVYCLGLCSVGPNAMAEGKVHARLDASKLGALVESLA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788621, Length=144, Percent_Identity=28.4722222222222, Blast_Score=62, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 14564; Mature: 14564

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLEQIIASHAGRQGALLPILHDVQAAFGHVSEDAIRAVASALNLSRADVFGVVSFYHD
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
FRQSPEPRAVLKLCRAEACQARGVEALAAQLPDNPHVKIEAVYCLGLCSVGPNAMAEGKV
HHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEHHEEHHHHHCCCCCCCCCCH
HARLDASKLGALVESLA
HHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MERLEQIIASHAGRQGALLPILHDVQAAFGHVSEDAIRAVASALNLSRADVFGVVSFYHD
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
FRQSPEPRAVLKLCRAEACQARGVEALAAQLPDNPHVKIEAVYCLGLCSVGPNAMAEGKV
HHCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEHHEEHHHHHCCCCCCCCCCH
HARLDASKLGALVESLA
HHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]