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The map label for this gene is nuoF2 [H]

Identifier: 87198757

GI number: 87198757

Start: 775058

End: 776596

Strand: Direct

Name: nuoF2 [H]

Synonym: Saro_0733

Alternate gene names: 87198757

Gene position: 775058-776596 (Clockwise)

Preceding gene: 87198756

Following gene: 87198758

Centisome position: 21.76

GC content: 66.47

Gene sequence:

>1539_bases
ATGACTGTTGTCCGAATTTCCGATGATGCGCTGGCGCTCGCCTGCGGGGCCGACGAAGTCGCGCAAGCCTTTGTCGCCGC
GGGCTGCACGGTGGAGCGTGTCTCTTCCTGGGGGATGCATTGGCTGGAGCCTCTGGCCGAAATCGACGGCCAGGGTTTCG
GTCCGCTCGAACCGGCGGACGTGGCGGCGGTGCTCGCTGGAACCTCGGAGAAGGCGGTGGGCCGGATCGAGGATCACCCC
TTCATTGCGCGCCAGCAGCGCTTCACTTTTGTGCGGGCGGGCCGGACCCGGCCGCTGAGCCTTGACGACTATGCCGCCAG
CGGCGGGTGGCAAGGTCTTGCCAGGGCGCGGGAAATCGGCCCGGTCTCGACGATCGCGCAGGTCACCGAATCCGGCCTTC
GCGGGCGCGGGGGCGCGGGGTTTCCGGCGGGGATCAAGTGGACCACGGTTGCAAATGCCTCTGGCGGAACGAAATACATC
GTCTGCAACGCCGACGAAGGCGACAGCGCGACCTTCGCCGACCGCATGGTGATGGAAGGCGATCCCTTCATGCTGATCGA
GGGCATGGCGATCGCCGGCTTCGCGGTGGGTGCGGGCAAGAGCTATGTCTACATCCGCAGCGAATATCCGCATGCCATTG
AAAAGATGGAAAAAGCGGTAAGACTGAGCGCTGCACTGGTCGCCCCTTTCGAAGTCGAGGTGCGGGTCGGCGCTGGGGCC
TATGTCTGCGGCGAGGAGACTTCGCTGCTCAATTCGCTGGAGGGCAAGCGCGGCGAAGTGCGGGCCAAGCCGCCGCTTCC
GGCGCTTGAGGGACTGTTCGGCAAGCCGACCGTCGTCAACAACGTTCTCACGCTCGCGGCGGTGCCGTTCATCCTCGCCC
AGGGCGCCCAAGAGTACGCCAATGTCGGTTTAGGTCGCTCTCGGGGCACGATGCCGATCCAGCTTGCCGGCAACGTCAGG
CACGGCGGTTTGTTCGAGGTGGGCTTCGGGATCACGCTGGGCGAACTGGTGAACGAGATCGGGGGCGGGACGGCTTCGGG
GCGGCCTGTCCGGGCGGTCCAGGTCGGCGGTCCCCTGGGCGCCTACCATCCGCCATCGGACTTCCACCTGCCGTTCGACT
ACGAAGCTTTCGCGGCGGCCGACGGCTTGATCGGCCATGCCGGCATCACGGTATTTGACGACACCGCCGACATGAGCGCG
CAGGCGCGCTTCGCGATGGAATTCTGCGCGGCGGAATCGTGCGGGAAATGCACGCCCTGCCGCATCGGATCGACGCGCGG
CGTCGAGCTGATCGACCGGATCCGCGCCCGCTCCGGCCGCCGCCCCGACCGGGTCGAGGCGCTGCCGCAGATGCACAATG
CCCGCAAGGCCGAACGCTCGCTCGCCGACGAGATCACCCTGCTCGAAGATTTGTGCGAGACGATGAAGTTCGGTTCGCTT
TGCGCGCTCGGAGGGTTCACGCCCTATCCGGTGCTGTCCGCGCTCAGGAACTTTCCCGAGGATTTTGGCGCCGCTCCGGG
CAAGGAAGCCGCCGAGTGA

Upstream 100 bases:

>100_bases
GTCTCGGGCTGTGCTCGGTGGGGCCGAACGCGATGGCGGAGGGCAAGGTCCACGCGCGGCTGGATGCGTCAAAGCTGGGC
GCGCTGGTGGAGTCGCTGGC

Downstream 100 bases:

>100_bases
TCCTTGGTGCCGTCCTTGCCGGTGGGCGGTCCACCCGCTTCGGCAGCGACAAGGCGCTGGCGGAGCTGGGTGGGCGCACG
TTGCTGGCGCGGGCCGTGGA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MTVVRISDDALALACGADEVAQAFVAAGCTVERVSSWGMHWLEPLAEIDGQGFGPLEPADVAAVLAGTSEKAVGRIEDHP
FIARQQRFTFVRAGRTRPLSLDDYAASGGWQGLARAREIGPVSTIAQVTESGLRGRGGAGFPAGIKWTTVANASGGTKYI
VCNADEGDSATFADRMVMEGDPFMLIEGMAIAGFAVGAGKSYVYIRSEYPHAIEKMEKAVRLSAALVAPFEVEVRVGAGA
YVCGEETSLLNSLEGKRGEVRAKPPLPALEGLFGKPTVVNNVLTLAAVPFILAQGAQEYANVGLGRSRGTMPIQLAGNVR
HGGLFEVGFGITLGELVNEIGGGTASGRPVRAVQVGGPLGAYHPPSDFHLPFDYEAFAAADGLIGHAGITVFDDTADMSA
QARFAMEFCAAESCGKCTPCRIGSTRGVELIDRIRARSGRRPDRVEALPQMHNARKAERSLADEITLLEDLCETMKFGSL
CALGGFTPYPVLSALRNFPEDFGAAPGKEAAE

Sequences:

>Translated_512_residues
MTVVRISDDALALACGADEVAQAFVAAGCTVERVSSWGMHWLEPLAEIDGQGFGPLEPADVAAVLAGTSEKAVGRIEDHP
FIARQQRFTFVRAGRTRPLSLDDYAASGGWQGLARAREIGPVSTIAQVTESGLRGRGGAGFPAGIKWTTVANASGGTKYI
VCNADEGDSATFADRMVMEGDPFMLIEGMAIAGFAVGAGKSYVYIRSEYPHAIEKMEKAVRLSAALVAPFEVEVRVGAGA
YVCGEETSLLNSLEGKRGEVRAKPPLPALEGLFGKPTVVNNVLTLAAVPFILAQGAQEYANVGLGRSRGTMPIQLAGNVR
HGGLFEVGFGITLGELVNEIGGGTASGRPVRAVQVGGPLGAYHPPSDFHLPFDYEAFAAADGLIGHAGITVFDDTADMSA
QARFAMEFCAAESCGKCTPCRIGSTRGVELIDRIRARSGRRPDRVEALPQMHNARKAERSLADEITLLEDLCETMKFGSL
CALGGFTPYPVLSALRNFPEDFGAAPGKEAAE
>Mature_511_residues
TVVRISDDALALACGADEVAQAFVAAGCTVERVSSWGMHWLEPLAEIDGQGFGPLEPADVAAVLAGTSEKAVGRIEDHPF
IARQQRFTFVRAGRTRPLSLDDYAASGGWQGLARAREIGPVSTIAQVTESGLRGRGGAGFPAGIKWTTVANASGGTKYIV
CNADEGDSATFADRMVMEGDPFMLIEGMAIAGFAVGAGKSYVYIRSEYPHAIEKMEKAVRLSAALVAPFEVEVRVGAGAY
VCGEETSLLNSLEGKRGEVRAKPPLPALEGLFGKPTVVNNVLTLAAVPFILAQGAQEYANVGLGRSRGTMPIQLAGNVRH
GGLFEVGFGITLGELVNEIGGGTASGRPVRAVQVGGPLGAYHPPSDFHLPFDYEAFAAADGLIGHAGITVFDDTADMSAQ
ARFAMEFCAAESCGKCTPCRIGSTRGVELIDRIRARSGRRPDRVEALPQMHNARKAERSLADEITLLEDLCETMKFGSLC
ALGGFTPYPVLSALRNFPEDFGAAPGKEAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=409, Percent_Identity=36.4303178484108, Blast_Score=227, Evalue=2e-59,
Organism=Homo sapiens, GI20149568, Length=409, Percent_Identity=36.4303178484108, Blast_Score=227, Evalue=3e-59,
Organism=Escherichia coli, GI1788620, Length=441, Percent_Identity=36.0544217687075, Blast_Score=262, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17531727, Length=410, Percent_Identity=36.5853658536585, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI19920794, Length=407, Percent_Identity=35.6265356265356, Blast_Score=215, Evalue=6e-56,
Organism=Drosophila melanogaster, GI45550452, Length=422, Percent_Identity=34.8341232227488, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24653819, Length=347, Percent_Identity=31.4121037463977, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24653817, Length=295, Percent_Identity=29.1525423728814, Blast_Score=122, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53912; Mature: 53781

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVRISDDALALACGADEVAQAFVAAGCTVERVSSWGMHWLEPLAEIDGQGFGPLEPAD
CEEEEECCCCEEEEECHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCHHH
VAAVLAGTSEKAVGRIEDHPFIARQQRFTFVRAGRTRPLSLDDYAASGGWQGLARAREIG
HHHHHCCCCHHHCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
PVSTIAQVTESGLRGRGGAGFPAGIKWTTVANASGGTKYIVCNADEGDSATFADRMVMEG
CHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHEEECC
DPFMLIEGMAIAGFAVGAGKSYVYIRSEYPHAIEKMEKAVRLSAALVAPFEVEVRVGAGA
CCEEEEECCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCE
YVCGEETSLLNSLEGKRGEVRAKPPLPALEGLFGKPTVVNNVLTLAAVPFILAQGAQEYA
EEECCHHHHHHHCCCCCCCEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHH
NVGLGRSRGTMPIQLAGNVRHGGLFEVGFGITLGELVNEIGGGTASGRPVRAVQVGGPLG
HCCCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCC
AYHPPSDFHLPFDYEAFAAADGLIGHAGITVFDDTADMSAQARFAMEFCAAESCGKCTPC
CCCCCCCCCCCCCCHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC
RIGSTRGVELIDRIRARSGRRPDRVEALPQMHNARKAERSLADEITLLEDLCETMKFGSL
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
CALGGFTPYPVLSALRNFPEDFGAAPGKEAAE
EECCCCCHHHHHHHHHCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
TVVRISDDALALACGADEVAQAFVAAGCTVERVSSWGMHWLEPLAEIDGQGFGPLEPAD
EEEEECCCCEEEEECHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCHHH
VAAVLAGTSEKAVGRIEDHPFIARQQRFTFVRAGRTRPLSLDDYAASGGWQGLARAREIG
HHHHHCCCCHHHCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCC
PVSTIAQVTESGLRGRGGAGFPAGIKWTTVANASGGTKYIVCNADEGDSATFADRMVMEG
CHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCHHHHHEEECC
DPFMLIEGMAIAGFAVGAGKSYVYIRSEYPHAIEKMEKAVRLSAALVAPFEVEVRVGAGA
CCEEEEECCEEEEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHEECCEEEEEEECCCE
YVCGEETSLLNSLEGKRGEVRAKPPLPALEGLFGKPTVVNNVLTLAAVPFILAQGAQEYA
EEECCHHHHHHHCCCCCCCEECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHH
NVGLGRSRGTMPIQLAGNVRHGGLFEVGFGITLGELVNEIGGGTASGRPVRAVQVGGPLG
HCCCCCCCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCEEEEEECCCCC
AYHPPSDFHLPFDYEAFAAADGLIGHAGITVFDDTADMSAQARFAMEFCAAESCGKCTPC
CCCCCCCCCCCCCCHHHHHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC
RIGSTRGVELIDRIRARSGRRPDRVEALPQMHNARKAERSLADEITLLEDLCETMKFGSL
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
CALGGFTPYPVLSALRNFPEDFGAAPGKEAAE
EECCCCCHHHHHHHHHCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]