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The map label for this gene is lcfA [H]

Identifier: 87198705

GI number: 87198705

Start: 717676

End: 719388

Strand: Direct

Name: lcfA [H]

Synonym: Saro_0681

Alternate gene names: 87198705

Gene position: 717676-719388 (Clockwise)

Preceding gene: 87198698

Following gene: 87198706

Centisome position: 20.15

GC content: 64.1

Gene sequence:

>1713_bases
ATGAAAACCGAGCTGGACCTTGCAATGGACCGCGCATTTGCCGCGCTGACGGCCGAAGGCGGCATGTTCCAAACGGTTCC
GATCCGGCGTTTCGGCCGCGACTTGCCGATGATTGCCGCTGCGCCTCCGCATCTTCCGGCCTATTTTGCCTATTTCTCAG
CCACTCACGGCGACAAGACCTTTATTGTCGACGGCGACCTGCGGCTGTCCTTTGCCGACGTCTACAAGGCCGCGCGCCAC
GTTGCGGGCGGACTGGTCGAAGGCATCGAGCTGCAGAAAGGCGAGCGTGTCGGTATCGCGGCGCGCAATTCCGCCAACTG
GATCATCGCCTACATGGCCGTGCTCATGGCCGGCGGCTGTGCGACCCTGCTCAATGGCTGGTGGCAGGGTGAGGAACTTG
CACACGGCATCGAGCTGGTCAGTTGCCGCTACGTGATTGCCGACAGCGCGCGCGCGGCCCGGCTCGAAGGTCATGCCCAT
GGTGCCACCGTGCTCCCGATGTCGCACGACTGCGGCTATGAGCAGGGCCTTGCCGCGCTGCTCGCCAGGGGCGGAGGCAT
GGAAACGCTCTTGCCGGACCTGACCGGGGACGATCTGGCGACGGTCCTGTTCACCTCGGGTTCGACTGGCACGGCCAAGG
GGGCCTATTCCGACCATCGTGGCGTCGTACAGGGGACGATGAACTACGTCTGCCAGTCGGCCGCTATGCTCGCCATCCTC
ACCGCCCGGGGCGAGGCGCCGGCTCCCGATGCGCAGCCGACCACGCTGGTCAACGTGCCCCTGTTCCACGTGACCGGCGA
AGTCCCGGTATTCCTTCAGTCGTTCGCGCTCGGTCGCAAGCTGGTGCTCATGGCCAAGTGGGATGCGGTCGACGCTATGC
GATTGATCGAAAAGGAAAAAGTCTCCTATTTCGTCGGCGTACCGCTGATGAGCTTCGAGATCGCCACCCACCCCGATCGC
GACAAATACGACCTGTCGACCTGCGTTTCCTTCGCAGCCGGCGGGGCGCCGCGCCCCGTCGAACACGTCGACCGGTTGCG
CAAGGCCTTGCCACACGCCTTCCCGCTCCTGGGCTACGGCCTGACCGAGACCAACGGAGTGGGCTGCGGCAACCTGAACG
AAAACTACCTCGCAAAGCCGGGTAGCACCGGCACCGCATCGCGGCCGCTGGTCGACCTCGCGATCCTCGACGATGCCGGC
AATCCGCTGCCGACCGGCGAAGTCGGGGAAGTCTCGATCCGTTCGATCGCCAATTTCCTGGGCTACTGGGATAACGAGAA
GGCCACGCGCGAGGCGATCACCCCCGATGGTTACTTCCGCAGCGGGGACCTCGGTTATCTCGATCCCGAGGGCTACCTGT
TCATCGTCGACCGCAAGAAGGACATCATCATCCGTGGCGGCGAGAATATCTCGTGCATCGAGGTGGAAAGCGCGATCTAC
GCCCACCCTTGCATCGCGGAAGCCAGCGTGTTCGGCCTGCCCGACGAAAAGTTCGGCGAAGTGCCGGCGGCGGTCTATCT
TGCCAAGGAAGGCTGTTCGGCCACCGATGACGAGTTGCGCACCTTCCTTGCCGAACATATCGCCCCGTTCAAGATTCCGG
TGCGTTTCTGGGAGGTCCACGAGGCGCTGCCCCGCCTTGGCACCGAGAAAGTCGACAAGCGCACTTTGCGCGAGCGCTAC
ACGCGGGAATGGCTCGCGAGCGCGACCGCCTGA

Upstream 100 bases:

>100_bases
CCTCGAGGGCGCCGTATTGGCACAGGGCAAAGCCCCCAGGCAAACCCGAAAACGGTCCGCGTACGACGGACGTTTCGGAC
CGGGAGATCGAAAGGACGCC

Downstream 100 bases:

>100_bases
GCAATGCACCTGAACGCAACAGTTGTTGGCCGGTAACATCGCAAGGTCTAAAGGGGGAGGCATGATGCTTCCCCGGATCG
CCAACCAGCGTGCCATCGTC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MKTELDLAMDRAFAALTAEGGMFQTVPIRRFGRDLPMIAAAPPHLPAYFAYFSATHGDKTFIVDGDLRLSFADVYKAARH
VAGGLVEGIELQKGERVGIAARNSANWIIAYMAVLMAGGCATLLNGWWQGEELAHGIELVSCRYVIADSARAARLEGHAH
GATVLPMSHDCGYEQGLAALLARGGGMETLLPDLTGDDLATVLFTSGSTGTAKGAYSDHRGVVQGTMNYVCQSAAMLAIL
TARGEAPAPDAQPTTLVNVPLFHVTGEVPVFLQSFALGRKLVLMAKWDAVDAMRLIEKEKVSYFVGVPLMSFEIATHPDR
DKYDLSTCVSFAAGGAPRPVEHVDRLRKALPHAFPLLGYGLTETNGVGCGNLNENYLAKPGSTGTASRPLVDLAILDDAG
NPLPTGEVGEVSIRSIANFLGYWDNEKATREAITPDGYFRSGDLGYLDPEGYLFIVDRKKDIIIRGGENISCIEVESAIY
AHPCIAEASVFGLPDEKFGEVPAAVYLAKEGCSATDDELRTFLAEHIAPFKIPVRFWEVHEALPRLGTEKVDKRTLRERY
TREWLASATA

Sequences:

>Translated_570_residues
MKTELDLAMDRAFAALTAEGGMFQTVPIRRFGRDLPMIAAAPPHLPAYFAYFSATHGDKTFIVDGDLRLSFADVYKAARH
VAGGLVEGIELQKGERVGIAARNSANWIIAYMAVLMAGGCATLLNGWWQGEELAHGIELVSCRYVIADSARAARLEGHAH
GATVLPMSHDCGYEQGLAALLARGGGMETLLPDLTGDDLATVLFTSGSTGTAKGAYSDHRGVVQGTMNYVCQSAAMLAIL
TARGEAPAPDAQPTTLVNVPLFHVTGEVPVFLQSFALGRKLVLMAKWDAVDAMRLIEKEKVSYFVGVPLMSFEIATHPDR
DKYDLSTCVSFAAGGAPRPVEHVDRLRKALPHAFPLLGYGLTETNGVGCGNLNENYLAKPGSTGTASRPLVDLAILDDAG
NPLPTGEVGEVSIRSIANFLGYWDNEKATREAITPDGYFRSGDLGYLDPEGYLFIVDRKKDIIIRGGENISCIEVESAIY
AHPCIAEASVFGLPDEKFGEVPAAVYLAKEGCSATDDELRTFLAEHIAPFKIPVRFWEVHEALPRLGTEKVDKRTLRERY
TREWLASATA
>Mature_570_residues
MKTELDLAMDRAFAALTAEGGMFQTVPIRRFGRDLPMIAAAPPHLPAYFAYFSATHGDKTFIVDGDLRLSFADVYKAARH
VAGGLVEGIELQKGERVGIAARNSANWIIAYMAVLMAGGCATLLNGWWQGEELAHGIELVSCRYVIADSARAARLEGHAH
GATVLPMSHDCGYEQGLAALLARGGGMETLLPDLTGDDLATVLFTSGSTGTAKGAYSDHRGVVQGTMNYVCQSAAMLAIL
TARGEAPAPDAQPTTLVNVPLFHVTGEVPVFLQSFALGRKLVLMAKWDAVDAMRLIEKEKVSYFVGVPLMSFEIATHPDR
DKYDLSTCVSFAAGGAPRPVEHVDRLRKALPHAFPLLGYGLTETNGVGCGNLNENYLAKPGSTGTASRPLVDLAILDDAG
NPLPTGEVGEVSIRSIANFLGYWDNEKATREAITPDGYFRSGDLGYLDPEGYLFIVDRKKDIIIRGGENISCIEVESAIY
AHPCIAEASVFGLPDEKFGEVPAAVYLAKEGCSATDDELRTFLAEHIAPFKIPVRFWEVHEALPRLGTEKVDKRTLRERY
TREWLASATA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=531, Percent_Identity=26.7419962335217, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI187761345, Length=594, Percent_Identity=24.9158249158249, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI187761343, Length=594, Percent_Identity=24.9158249158249, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI42544132, Length=526, Percent_Identity=24.9049429657795, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI122937307, Length=266, Percent_Identity=33.0827067669173, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI157311624, Length=522, Percent_Identity=24.7126436781609, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI157311622, Length=522, Percent_Identity=24.7126436781609, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI58082049, Length=529, Percent_Identity=24.1965973534972, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=23.8921001926782, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI38505220, Length=524, Percent_Identity=25.381679389313, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI83745141, Length=504, Percent_Identity=24.4047619047619, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI13375727, Length=164, Percent_Identity=29.2682926829268, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI28416953, Length=553, Percent_Identity=22.7848101265823, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI27477105, Length=503, Percent_Identity=22.8628230616302, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1788107, Length=545, Percent_Identity=26.605504587156, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI145693145, Length=370, Percent_Identity=31.6216216216216, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=24.8520710059172, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1786810, Length=533, Percent_Identity=28.3302063789869, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788595, Length=375, Percent_Identity=26.4, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790505, Length=559, Percent_Identity=23.7924865831843, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=368, Percent_Identity=29.8913043478261, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994694, Length=368, Percent_Identity=29.8913043478261, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71994690, Length=368, Percent_Identity=29.8913043478261, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17560308, Length=513, Percent_Identity=26.3157894736842, Blast_Score=154, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17558820, Length=513, Percent_Identity=26.7056530214425, Blast_Score=152, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI17531443, Length=513, Percent_Identity=24.7563352826511, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32563687, Length=515, Percent_Identity=26.0194174757282, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17559526, Length=377, Percent_Identity=28.6472148541114, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=540, Percent_Identity=24.2592592592593, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=25.2941176470588, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=23.3918128654971, Blast_Score=118, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71985884, Length=398, Percent_Identity=25.6281407035176, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71996755, Length=452, Percent_Identity=21.2389380530973, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=497, Percent_Identity=29.5774647887324, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320852, Length=377, Percent_Identity=27.8514588859416, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=199, Percent_Identity=30.1507537688442, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355181, Length=534, Percent_Identity=27.1535580524345, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=27.8719397363465, Blast_Score=158, Evalue=8e-39,
Organism=Drosophila melanogaster, GI18859661, Length=566, Percent_Identity=24.5583038869258, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=25.2525252525253, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24656500, Length=506, Percent_Identity=25.296442687747, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21358303, Length=514, Percent_Identity=23.3463035019455, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648260, Length=493, Percent_Identity=26.1663286004057, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24648253, Length=317, Percent_Identity=28.7066246056782, Blast_Score=116, Evalue=5e-26,
Organism=Drosophila melanogaster, GI24648255, Length=317, Percent_Identity=29.0220820189274, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI281365686, Length=251, Percent_Identity=29.4820717131474, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24653035, Length=487, Percent_Identity=24.0246406570842, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21356441, Length=473, Percent_Identity=22.8329809725159, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161076582, Length=500, Percent_Identity=24.4, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356947, Length=347, Percent_Identity=24.4956772334294, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI20130357, Length=334, Percent_Identity=25.1497005988024, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21355225, Length=543, Percent_Identity=23.7569060773481, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921316, Length=544, Percent_Identity=24.4485294117647, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648257, Length=531, Percent_Identity=21.8455743879473, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17933690, Length=488, Percent_Identity=21.3114754098361, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 61554; Mature: 61554

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTELDLAMDRAFAALTAEGGMFQTVPIRRFGRDLPMIAAAPPHLPAYFAYFSATHGDKT
CCCCHHHHHHHHHHHEECCCCEEEECCHHHCCCCCCEEECCCCCCHHHHHHHEECCCCEE
FIVDGDLRLSFADVYKAARHVAGGLVEGIELQKGERVGIAARNSANWIIAYMAVLMAGGC
EEEECCCEEHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCHHHHHHHHHHHCCH
ATLLNGWWQGEELAHGIELVSCRYVIADSARAARLEGHAHGATVLPMSHDCGYEQGLAAL
HHHHCCCCCCHHHHCCCEEEEEEEEEECCCCHHEECCCCCCEEEEECCCCCCHHHHHHHH
LARGGGMETLLPDLTGDDLATVLFTSGSTGTAKGAYSDHRGVVQGTMNYVCQSAAMLAIL
HHCCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
TARGEAPAPDAQPTTLVNVPLFHVTGEVPVFLQSFALGRKLVLMAKWDAVDAMRLIEKEK
EECCCCCCCCCCCCEEEECEEEEECCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
VSYFVGVPLMSFEIATHPDRDKYDLSTCVSFAAGGAPRPVEHVDRLRKALPHAFPLLGYG
CEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHCCC
LTETNGVGCGNLNENYLAKPGSTGTASRPLVDLAILDDAGNPLPTGEVGEVSIRSIANFL
CCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
GYWDNEKATREAITPDGYFRSGDLGYLDPEGYLFIVDRKKDIIIRGGENISCIEVESAIY
CCCCCCCHHHHCCCCCCCEECCCCCEECCCCEEEEEECCCEEEEECCCCEEEEEECCHHH
AHPCIAEASVFGLPDEKFGEVPAAVYLAKEGCSATDDELRTFLAEHIAPFKIPVRFWEVH
CCCCHHHHHCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHH
EALPRLGTEKVDKRTLRERYTREWLASATA
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTELDLAMDRAFAALTAEGGMFQTVPIRRFGRDLPMIAAAPPHLPAYFAYFSATHGDKT
CCCCHHHHHHHHHHHEECCCCEEEECCHHHCCCCCCEEECCCCCCHHHHHHHEECCCCEE
FIVDGDLRLSFADVYKAARHVAGGLVEGIELQKGERVGIAARNSANWIIAYMAVLMAGGC
EEEECCCEEHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEECCCCCHHHHHHHHHHHCCH
ATLLNGWWQGEELAHGIELVSCRYVIADSARAARLEGHAHGATVLPMSHDCGYEQGLAAL
HHHHCCCCCCHHHHCCCEEEEEEEEEECCCCHHEECCCCCCEEEEECCCCCCHHHHHHHH
LARGGGMETLLPDLTGDDLATVLFTSGSTGTAKGAYSDHRGVVQGTMNYVCQSAAMLAIL
HHCCCCCHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEE
TARGEAPAPDAQPTTLVNVPLFHVTGEVPVFLQSFALGRKLVLMAKWDAVDAMRLIEKEK
EECCCCCCCCCCCCEEEECEEEEECCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHH
VSYFVGVPLMSFEIATHPDRDKYDLSTCVSFAAGGAPRPVEHVDRLRKALPHAFPLLGYG
CEEEECCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHCCC
LTETNGVGCGNLNENYLAKPGSTGTASRPLVDLAILDDAGNPLPTGEVGEVSIRSIANFL
CCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHH
GYWDNEKATREAITPDGYFRSGDLGYLDPEGYLFIVDRKKDIIIRGGENISCIEVESAIY
CCCCCCCHHHHCCCCCCCEECCCCCEECCCCEEEEEECCCEEEEECCCCEEEEEECCHHH
AHPCIAEASVFGLPDEKFGEVPAAVYLAKEGCSATDDELRTFLAEHIAPFKIPVRFWEVH
CCCCHHHHHCCCCCCHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHH
EALPRLGTEKVDKRTLRERYTREWLASATA
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]