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The map label for this gene is cbdA [H]

Identifier: 87198562

GI number: 87198562

Start: 587562

End: 588896

Strand: Direct

Name: cbdA [H]

Synonym: Saro_0538

Alternate gene names: 87198562

Gene position: 587562-588896 (Clockwise)

Preceding gene: 87198560

Following gene: 87198563

Centisome position: 16.5

GC content: 63.52

Gene sequence:

>1335_bases
ATGTCCGCCGCGCTGAAAACCCGCATTGAAACGGCCGTCGTCGATGACGCGGAAACCGGGCAGTTCCGCTGCCGCCGCGA
CATCTTCACCGATCCCGACCTGTTCGAACTCGAGATGAAGCACATCTTCGAGGGGAATTGGGTCTACCTGGCGCACGAGA
GCCAGATCCCCGCCGCCAACGACTGGTTCGCGGTCACGGTGGGGCGGACGCCGGTGGTCATCACGCGCACGAAGGATGGC
TCGTTCAACGCCTTCATCAATGCGTGTTCTCACCGTGGCGCGATGTTGTGCCGTCGCAAGCGGGGCAACGGTCGGGTCAT
GGTCTGCCCGTTCCACGGCTGGAGCTTCACCAACGACGGCAAGCTGCTGAAGGCAAAGGACGAAGGTTCGGGGGCCTATC
CGGAGTCGTTCAACCGCGATGGTTCGCACGATCTTGCGCGGCTGGCGCGGTTCGAGAGCTATCGCGGCTTCCTGTTCGGC
AGTGTCAATCCGGACGTCGCGCCGCTGGCGGAATACCTTGGCGAGACGCGGGTGATAATCGACCAGATCGTCGATCAGGC
GCCGGAGGGCATTGAGGTGCTGGCCGGAAGCTCTTCCTATATCTTCGACGGCAACTGGAAGTTGCAGATGGAGAACGGGT
GCGACGGATATCACGTCAGCTCGGTCCACTACAATTACGCCAGGACAATGGGGCGGCGCGCCGAGGGCGGCACCAAGGCA
GTGGACGCCAATGGCTGGTCGAAGGCAGTCAGCGGCGTCTACGGGTTCGACAACGGGCACATCCTGCTGTGGACGCGGGT
GCTCAATCCCGAGGTCCGGCCGGTGTGGTCGCGCAAGGATGAGCTGGAGCAGCGGCTGGGCAAGGAGCGCACGCGCTTCA
TCGTCGAGCAGAGCCGCAACCTGGCGCTCTATCCGAACGTCTTCCTGATGGACCAGTTCTCGACCCAGATCCGCGTGGTA
CGCCCGATCGATGCCGACCACACCGAAGTGACGATCTACTGCTTTGCGCCCAAGGGGGAGAGCCCGGAACTGCGGGCTGT
CCGCATCCGCCAGTACGAGGACTTCTTCAACGTCTCGGGCATGGGCACGCCGGATGATCTCGAGGAGTTCCGTACTTGCC
AGGCCTCCTATGCGGGTGCGGGCGGGTTGTGGAACGACCTCAGCCGCGGCGCGCGGCGCTGGATTGCCGGGCCGGACGAA
AATGCGCGCGAAATGGGCTTGAACCCGTTGCTCAGCAGCGAGCGCAGCGAGGATGAAGGCTTGTTCGTCCGCCAGCACGA
ATACTGGGCGAAGACCCTTCTCGAAGGCCTGGCCCGGCAGGAGGAAATGGCATGA

Upstream 100 bases:

>100_bases
CGGCATTCGACTTCGGTTCGCAGCAGGCCCCGCGCCGAGAGGACGAGCGGTCGCCGAACCACGCTCGTCCTGAAGCCTGA
CGAGAGAAGGAACGCCAAGC

Downstream 100 bases:

>100_bases
GCGCGCTCGATGAAGTCTGCGCCTTCCTCCACCGCGAGGCGCGCCTGCTCGATGACCGGCAGTTGGATGTCTGGCTGGAA
TGCTATGCGCCCGATTGCAC

Product: Rieske (2Fe-2S) protein

Products: NA

Alternate protein names: 2-chlorobenzoate 1,2-dioxygenase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSAALKTRIETAVVDDAETGQFRCRRDIFTDPDLFELEMKHIFEGNWVYLAHESQIPAANDWFAVTVGRTPVVITRTKDG
SFNAFINACSHRGAMLCRRKRGNGRVMVCPFHGWSFTNDGKLLKAKDEGSGAYPESFNRDGSHDLARLARFESYRGFLFG
SVNPDVAPLAEYLGETRVIIDQIVDQAPEGIEVLAGSSSYIFDGNWKLQMENGCDGYHVSSVHYNYARTMGRRAEGGTKA
VDANGWSKAVSGVYGFDNGHILLWTRVLNPEVRPVWSRKDELEQRLGKERTRFIVEQSRNLALYPNVFLMDQFSTQIRVV
RPIDADHTEVTIYCFAPKGESPELRAVRIRQYEDFFNVSGMGTPDDLEEFRTCQASYAGAGGLWNDLSRGARRWIAGPDE
NAREMGLNPLLSSERSEDEGLFVRQHEYWAKTLLEGLARQEEMA

Sequences:

>Translated_444_residues
MSAALKTRIETAVVDDAETGQFRCRRDIFTDPDLFELEMKHIFEGNWVYLAHESQIPAANDWFAVTVGRTPVVITRTKDG
SFNAFINACSHRGAMLCRRKRGNGRVMVCPFHGWSFTNDGKLLKAKDEGSGAYPESFNRDGSHDLARLARFESYRGFLFG
SVNPDVAPLAEYLGETRVIIDQIVDQAPEGIEVLAGSSSYIFDGNWKLQMENGCDGYHVSSVHYNYARTMGRRAEGGTKA
VDANGWSKAVSGVYGFDNGHILLWTRVLNPEVRPVWSRKDELEQRLGKERTRFIVEQSRNLALYPNVFLMDQFSTQIRVV
RPIDADHTEVTIYCFAPKGESPELRAVRIRQYEDFFNVSGMGTPDDLEEFRTCQASYAGAGGLWNDLSRGARRWIAGPDE
NAREMGLNPLLSSERSEDEGLFVRQHEYWAKTLLEGLARQEEMA
>Mature_443_residues
SAALKTRIETAVVDDAETGQFRCRRDIFTDPDLFELEMKHIFEGNWVYLAHESQIPAANDWFAVTVGRTPVVITRTKDGS
FNAFINACSHRGAMLCRRKRGNGRVMVCPFHGWSFTNDGKLLKAKDEGSGAYPESFNRDGSHDLARLARFESYRGFLFGS
VNPDVAPLAEYLGETRVIIDQIVDQAPEGIEVLAGSSSYIFDGNWKLQMENGCDGYHVSSVHYNYARTMGRRAEGGTKAV
DANGWSKAVSGVYGFDNGHILLWTRVLNPEVRPVWSRKDELEQRLGKERTRFIVEQSRNLALYPNVFLMDQFSTQIRVVR
PIDADHTEVTIYCFAPKGESPELRAVRIRQYEDFFNVSGMGTPDDLEEFRTCQASYAGAGGLWNDLSRGARRWIAGPDEN
AREMGLNPLLSSERSEDEGLFVRQHEYWAKTLLEGLARQEEMA

Specific function: Component of 2-halobenzoate dioxygenase multicomponent enzyme system which catalyzes the incorporation of both atoms of molecular oxygen into 2-halobenzoate to form catechol [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=381, Percent_Identity=30.1837270341207, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788103, Length=204, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.13 [H]

Molecular weight: Translated: 50155; Mature: 50024

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00570 RING_HYDROXYL_ALPHA ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAALKTRIETAVVDDAETGQFRCRRDIFTDPDLFELEMKHIFEGNWVYLAHESQIPAAN
CCCHHHHHHHHEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCC
DWFAVTVGRTPVVITRTKDGSFNAFINACSHRGAMLCRRKRGNGRVMVCPFHGWSFTNDG
CEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCC
KLLKAKDEGSGAYPESFNRDGSHDLARLARFESYRGFLFGSVNPDVAPLAEYLGETRVII
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHHHHH
DQIVDQAPEGIEVLAGSSSYIFDGNWKLQMENGCDGYHVSSVHYNYARTMGRRAEGGTKA
HHHHHCCCCCEEEEECCCCEEECCCEEEEECCCCCCEEEEEEEHHHHHHHCCCCCCCCEE
VDANGWSKAVSGVYGFDNGHILLWTRVLNPEVRPVWSRKDELEQRLGKERTRFIVEQSRN
ECCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LALYPNVFLMDQFSTQIRVVRPIDADHTEVTIYCFAPKGESPELRAVRIRQYEDFFNVSG
EEECCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEEEHHHHHHCCCC
MGTPDDLEEFRTCQASYAGAGGLWNDLSRGARRWIAGPDENAREMGLNPLLSSERSEDEG
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCHHCCCCCCCCC
LFVRQHEYWAKTLLEGLARQEEMA
EEEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAALKTRIETAVVDDAETGQFRCRRDIFTDPDLFELEMKHIFEGNWVYLAHESQIPAAN
CCHHHHHHHHEEECCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCC
DWFAVTVGRTPVVITRTKDGSFNAFINACSHRGAMLCRRKRGNGRVMVCPFHGWSFTNDG
CEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCC
KLLKAKDEGSGAYPESFNRDGSHDLARLARFESYRGFLFGSVNPDVAPLAEYLGETRVII
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHCCHHHHH
DQIVDQAPEGIEVLAGSSSYIFDGNWKLQMENGCDGYHVSSVHYNYARTMGRRAEGGTKA
HHHHHCCCCCEEEEECCCCEEECCCEEEEECCCCCCEEEEEEEHHHHHHHCCCCCCCCEE
VDANGWSKAVSGVYGFDNGHILLWTRVLNPEVRPVWSRKDELEQRLGKERTRFIVEQSRN
ECCCCHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LALYPNVFLMDQFSTQIRVVRPIDADHTEVTIYCFAPKGESPELRAVRIRQYEDFFNVSG
EEECCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEEEEEEHHHHHHCCCC
MGTPDDLEEFRTCQASYAGAGGLWNDLSRGARRWIAGPDENAREMGLNPLLSSERSEDEG
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHCCCCHHCCCCCCCCC
LFVRQHEYWAKTLLEGLARQEEMA
EEEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7530709; 1370284 [H]