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The map label for this gene is ruvB

Identifier: 87198448

GI number: 87198448

Start: 459887

End: 460915

Strand: Direct

Name: ruvB

Synonym: Saro_0423

Alternate gene names: 87198448

Gene position: 459887-460915 (Clockwise)

Preceding gene: 87198447

Following gene: 87198449

Centisome position: 12.91

GC content: 66.96

Gene sequence:

>1029_bases
ATGACCGACAACCCGCTCCTTTCCTCCGCCTCGCAGATCGAGGATCAGGACGCCGCGCTGCGGCCCAAGACGCTGGCCGA
GTTCGTCGGCCAGGCCGGGGCCAAGGACAACCTGCAGGTCTTCATCGAAAGCGCCAAGCAGCGGCGCGAGGCGATGGACC
ATGTGCTGTTCTTCGGCCCTCCCGGCCTTGGCAAGACGACGCTGGCGCAGATCATTGCGCGCGAACTGGGCGTCAATTTC
CGTGCCACGTCCGGCCCGGTCATCGCCAAGGCGGGGGACCTCGCCGCGCTGCTAACCAATCTCGAACATGGCGACGTCCT
GTTCATCGACGAGATCCACCGCCTCAATCCCGTGGTCGAGGAAGTGCTCTATCCGGCGATGGAGGACCGCGCGCTTGATC
TCATCATCGGGGAAGGTCCCTCGGCGCGATCTGTGCGGATCGATCTGCCACCCTTCACCCTGATCGGCGCGACCACGCGC
CAGGGCCTGTTGCAGACGCCGCTGCGCGACCGTTTCGGCATTCCGGTGCGCCTGCAGTTCTATTCGGTCGAGGAACTTGA
GCGCGTGGTCGCGCGCGGGGCGAGCCTGATGGGCATCGGCATCGACAAGGGCGGGGCGACCGAGATTGCCCGTCGCTCGC
GCGGGACGCCGCGTGTCGCCGGCCGCCTGCTGCGGCGGGTGCGCGACTTCGCGCAGGTTGCGGGCGCGGAGAAGATCACA
CAATCGATCGCCGACAATGCTTTGACCCGGCTGGAGGTCGACAAGATCGGCCTCGATCTTCAGGATCGCCGCTATCTCAC
CATGATCGCGGACATCTACAAGGGCGGGCCGGTGGGCGTGGAGACCCTGGCAGCGGGGCTTTCCGAACCGCGCGACACGA
TCGAGGAAGTGATCGAGCCCTACCTGATCCAGCTCGGTATGATCGCCCGCACCGCGCGCGGGCGGTGCCTCAACGACCGT
GGCTGGCAGCATCTCGGCCTTGCTCCGCCCTCGGGCCAGATGCCGGGGCTGTTCGGCCCGGACGAGTGA

Upstream 100 bases:

>100_bases
CGCCAGCAGCGCCGTCGCCGCAGCAGTCAAGGAACTGGGCGAGGATGCGGGGTTGAACGACCTGGTGCGCGTGGCGCTGA
AGCGGGCGGCGGGATAACGA

Downstream 100 bases:

>100_bases
CCGGTTAAGGACTTCCCGGCGGATCGGTCCCATATTGGCACAGAGTGGGCCCGGGCCGGTCGATGGTCCCGTTTTCGGGC
AGATTCAACACCCGGAATCC

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTDNPLLSSASQIEDQDAALRPKTLAEFVGQAGAKDNLQVFIESAKQRREAMDHVLFFGPPGLGKTTLAQIIARELGVNF
RATSGPVIAKAGDLAALLTNLEHGDVLFIDEIHRLNPVVEEVLYPAMEDRALDLIIGEGPSARSVRIDLPPFTLIGATTR
QGLLQTPLRDRFGIPVRLQFYSVEELERVVARGASLMGIGIDKGGATEIARRSRGTPRVAGRLLRRVRDFAQVAGAEKIT
QSIADNALTRLEVDKIGLDLQDRRYLTMIADIYKGGPVGVETLAAGLSEPRDTIEEVIEPYLIQLGMIARTARGRCLNDR
GWQHLGLAPPSGQMPGLFGPDE

Sequences:

>Translated_342_residues
MTDNPLLSSASQIEDQDAALRPKTLAEFVGQAGAKDNLQVFIESAKQRREAMDHVLFFGPPGLGKTTLAQIIARELGVNF
RATSGPVIAKAGDLAALLTNLEHGDVLFIDEIHRLNPVVEEVLYPAMEDRALDLIIGEGPSARSVRIDLPPFTLIGATTR
QGLLQTPLRDRFGIPVRLQFYSVEELERVVARGASLMGIGIDKGGATEIARRSRGTPRVAGRLLRRVRDFAQVAGAEKIT
QSIADNALTRLEVDKIGLDLQDRRYLTMIADIYKGGPVGVETLAAGLSEPRDTIEEVIEPYLIQLGMIARTARGRCLNDR
GWQHLGLAPPSGQMPGLFGPDE
>Mature_341_residues
TDNPLLSSASQIEDQDAALRPKTLAEFVGQAGAKDNLQVFIESAKQRREAMDHVLFFGPPGLGKTTLAQIIARELGVNFR
ATSGPVIAKAGDLAALLTNLEHGDVLFIDEIHRLNPVVEEVLYPAMEDRALDLIIGEGPSARSVRIDLPPFTLIGATTRQ
GLLQTPLRDRFGIPVRLQFYSVEELERVVARGASLMGIGIDKGGATEIARRSRGTPRVAGRLLRRVRDFAQVAGAEKITQ
SIADNALTRLEVDKIGLDLQDRRYLTMIADIYKGGPVGVETLAAGLSEPRDTIEEVIEPYLIQLGMIARTARGRCLNDRG
WQHLGLAPPSGQMPGLFGPDE

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=319, Percent_Identity=61.128526645768, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1787119, Length=134, Percent_Identity=33.5820895522388, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_NOVAD (Q2GBA2)

Other databases:

- EMBL:   CP000248
- RefSeq:   YP_495705.1
- ProteinModelPortal:   Q2GBA2
- SMR:   Q2GBA2
- STRING:   Q2GBA2
- GeneID:   3917569
- GenomeReviews:   CP000248_GR
- KEGG:   nar:Saro_0423
- NMPDR:   fig|48935.1.peg.3721
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- PhylomeDB:   Q2GBA2
- ProtClustDB:   PRK00080
- BioCyc:   NARO279238:SARO_0423-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 37186; Mature: 37055

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNPLLSSASQIEDQDAALRPKTLAEFVGQAGAKDNLQVFIESAKQRREAMDHVLFFGP
CCCCCCCCCHHHHCCCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEECC
PGLGKTTLAQIIARELGVNFRATSGPVIAKAGDLAALLTNLEHGDVLFIDEIHRLNPVVE
CCCCHHHHHHHHHHHHCCCEEECCCCEEECCCHHHHHHHCCCCCCEEEEHHHHHHHHHHH
EVLYPAMEDRALDLIIGEGPSARSVRIDLPPFTLIGATTRQGLLQTPLRDRFGIPVRLQF
HHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEECCHHHHHHHHCCHHHHCCCCEEEEE
YSVEELERVVARGASLMGIGIDKGGATEIARRSRGTPRVAGRLLRRVRDFAQVAGAEKIT
ECHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSIADNALTRLEVDKIGLDLQDRRYLTMIADIYKGGPVGVETLAAGLSEPRDTIEEVIEP
HHHHHHHHHHEEHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
YLIQLGMIARTARGRCLNDRGWQHLGLAPPSGQMPGLFGPDE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDNPLLSSASQIEDQDAALRPKTLAEFVGQAGAKDNLQVFIESAKQRREAMDHVLFFGP
CCCCCCCCHHHHCCCHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEECC
PGLGKTTLAQIIARELGVNFRATSGPVIAKAGDLAALLTNLEHGDVLFIDEIHRLNPVVE
CCCCHHHHHHHHHHHHCCCEEECCCCEEECCCHHHHHHHCCCCCCEEEEHHHHHHHHHHH
EVLYPAMEDRALDLIIGEGPSARSVRIDLPPFTLIGATTRQGLLQTPLRDRFGIPVRLQF
HHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEECCHHHHHHHHCCHHHHCCCCEEEEE
YSVEELERVVARGASLMGIGIDKGGATEIARRSRGTPRVAGRLLRRVRDFAQVAGAEKIT
ECHHHHHHHHHCCCCEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSIADNALTRLEVDKIGLDLQDRRYLTMIADIYKGGPVGVETLAAGLSEPRDTIEEVIEP
HHHHHHHHHHEEHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHH
YLIQLGMIARTARGRCLNDRGWQHLGLAPPSGQMPGLFGPDE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA