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The map label for this gene is fieF [H]

Identifier: 86750546

GI number: 86750546

Start: 3933915

End: 3935351

Strand: Direct

Name: fieF [H]

Synonym: RPB_3435

Alternate gene names: 86750546

Gene position: 3933915-3935351 (Clockwise)

Preceding gene: 86750545

Following gene: 86750547

Centisome position: 73.78

GC content: 67.99

Gene sequence:

>1437_bases
ATGGCGATCCGCTTCGGCCGGCTTTTATGTTCAGGATCGGGCTGCTACACCGCGCCCATGAGCAATTTCGCCAGCACCAA
ATCGCGTGTGGCTGCGGTGTCCGTGCTGGCGAGCGCCAGCATGGCCGCGGCGAAATTCGCCGTCGGCATCGCCATCGGAT
CGCTGGCGCTGATCTCCGAGGCGCTGCACAGTTCGATCGATCTGGTCGCGACCATCATCACCTGGTTCGTGGTCCGGGTG
TCCGACCGCCCGGCCGACGATGCCCATCACTACGGCCACGGCAAGATCGAAAGCCTGTCGGCGCTCGGCGTCATCGCATT
GCTGTATGTCCTGGCGGGCGGCATTCTGGTCGAGGCCACAAGCCGGTTGCGCGAGGGCGCTCCGCCGCCGGCCTTCTCGC
TGTGGGCCTTCGGTATTCTCGGCGTCGACATCCTGGTGAATTCCTGGCGCGCCTGGGCGTTGCATCGCACGGCGGTCGCG
ACCAAGAGCCAGGCGCTCGCCGCCGACGCGCTGCACTTCGCCTCCGACGTGCTCGGCTCGTTCGCGGTGATCGCCGGACT
CTCGCTGTCGGCGCTGGGCTTCGCCTGGGGCGACGCCGCCGCCGCGATCGGCGTCGCGGTGATGATCTCGATCCTCGGCC
TGCGGCTCGGCCGCTCGACGATCCAGACCCTGCTCGACCGCGCGCCGGAGGGCGTCTCGGACAAGGCCCGCGACGTGATC
AGCGCGGTGCCCGGCGTCGTCGGCATCGAACGGCTGCGCACCCGCATGGTCGGGCCGACGCATTTCATCGACGCGCTGGT
CGAGGTGCCGCGCACCTACCCGATCGACCGCATCGACGAGATCAAGCGCCGCGCCGACGACGCGCTGAAGGGCGCTTTCG
GCGATTCCGACCTCACCTTCACGGCGATCCCGGTCGCCCGCGACAACGAGACCGTGCGCGAGCGCATCATGGTGATCGCG
CGCAATTCCGGCCTCGCCGTCCACCACGTCACCGTCCACGACCTCGGCTGCAAGATCACCGTCAGCATCGATCTCGAGGT
CGACGGCGACATGCCGCTGATCGAGGCGCACGACATCGCCCACGAACTGGAGCGGCACATCCGCGACGATTTCGGCACCG
ACGTCGAGGTCGACACCCATATCGAGCCGCTCGAGCCGGAAATGCCGCACGGCTACGAAGCCGCGCCGGAACGCGTCGAG
GAAATTCGCGCGGCGCTGATCGGCTTCGCAAAGAACGGCGCGATCCACGACATCCACAGCGTCCGGGTCCGCGACACCGA
GGCCGGCGAGATCGTCAACTTCCACTGCTACGCCGAGCCGTCGCTCAGCGTCATCGCGGTGCACGACCACGTCGACGAGA
TCGAGCGCCATATGCGGCGCGCGTTCCCGAGCGTGAAGCGAGTCATCAGCCACGCCGAGCCGCCGAACGCGGACTGA

Upstream 100 bases:

>100_bases
TCGGTAAGATCGTGGTGACGTTCTGAGCGGTTCCCGCAATCGCCACGACGCAGCCGCTTGAATAAGAACAATTTGCAATA
ACGGCTGTCGCAAAATCGCG

Downstream 100 bases:

>100_bases
TCGCCGCGCCTGCGACTCCTACGAGTCTCCGTTCTCGTTTCGGACTCGGCTCACGCACAAATGAGAGCGCGATTCATCTT
TGGATAAGAAATTATCCGAT

Product: cation diffusion facilitator family transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAIRFGRLLCSGSGCYTAPMSNFASTKSRVAAVSVLASASMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVRV
SDRPADDAHHYGHGKIESLSALGVIALLYVLAGGILVEATSRLREGAPPPAFSLWAFGILGVDILVNSWRAWALHRTAVA
TKSQALAADALHFASDVLGSFAVIAGLSLSALGFAWGDAAAAIGVAVMISILGLRLGRSTIQTLLDRAPEGVSDKARDVI
SAVPGVVGIERLRTRMVGPTHFIDALVEVPRTYPIDRIDEIKRRADDALKGAFGDSDLTFTAIPVARDNETVRERIMVIA
RNSGLAVHHVTVHDLGCKITVSIDLEVDGDMPLIEAHDIAHELERHIRDDFGTDVEVDTHIEPLEPEMPHGYEAAPERVE
EIRAALIGFAKNGAIHDIHSVRVRDTEAGEIVNFHCYAEPSLSVIAVHDHVDEIERHMRRAFPSVKRVISHAEPPNAD

Sequences:

>Translated_478_residues
MAIRFGRLLCSGSGCYTAPMSNFASTKSRVAAVSVLASASMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVRV
SDRPADDAHHYGHGKIESLSALGVIALLYVLAGGILVEATSRLREGAPPPAFSLWAFGILGVDILVNSWRAWALHRTAVA
TKSQALAADALHFASDVLGSFAVIAGLSLSALGFAWGDAAAAIGVAVMISILGLRLGRSTIQTLLDRAPEGVSDKARDVI
SAVPGVVGIERLRTRMVGPTHFIDALVEVPRTYPIDRIDEIKRRADDALKGAFGDSDLTFTAIPVARDNETVRERIMVIA
RNSGLAVHHVTVHDLGCKITVSIDLEVDGDMPLIEAHDIAHELERHIRDDFGTDVEVDTHIEPLEPEMPHGYEAAPERVE
EIRAALIGFAKNGAIHDIHSVRVRDTEAGEIVNFHCYAEPSLSVIAVHDHVDEIERHMRRAFPSVKRVISHAEPPNAD
>Mature_477_residues
AIRFGRLLCSGSGCYTAPMSNFASTKSRVAAVSVLASASMAAAKFAVGIAIGSLALISEALHSSIDLVATIITWFVVRVS
DRPADDAHHYGHGKIESLSALGVIALLYVLAGGILVEATSRLREGAPPPAFSLWAFGILGVDILVNSWRAWALHRTAVAT
KSQALAADALHFASDVLGSFAVIAGLSLSALGFAWGDAAAAIGVAVMISILGLRLGRSTIQTLLDRAPEGVSDKARDVIS
AVPGVVGIERLRTRMVGPTHFIDALVEVPRTYPIDRIDEIKRRADDALKGAFGDSDLTFTAIPVARDNETVRERIMVIAR
NSGLAVHHVTVHDLGCKITVSIDLEVDGDMPLIEAHDIAHELERHIRDDFGTDVEVDTHIEPLEPEMPHGYEAAPERVEE
IRAALIGFAKNGAIHDIHSVRVRDTEAGEIVNFHCYAEPSLSVIAVHDHVDEIERHMRRAFPSVKRVISHAEPPNAD

Specific function: Cation-efflux transporter that may have a role in detoxification [H]

COG id: COG0053

COG function: function code P; Predicted Co/Zn/Cd cation transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. FieF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790349, Length=261, Percent_Identity=32.5670498084291, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002524 [H]

Pfam domain/function: PF01545 Cation_efflux [H]

EC number: NA

Molecular weight: Translated: 51295; Mature: 51164

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRFGRLLCSGSGCYTAPMSNFASTKSRVAAVSVLASASMAAAKFAVGIAIGSLALISE
CCEEECCCEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALHSSIDLVATIITWFVVRVSDRPADDAHHYGHGKIESLSALGVIALLYVLAGGILVEAT
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
SRLREGAPPPAFSLWAFGILGVDILVNSWRAWALHRTAVATKSQALAADALHFASDVLGS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAVIAGLSLSALGFAWGDAAAAIGVAVMISILGLRLGRSTIQTLLDRAPEGVSDKARDVI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHH
SAVPGVVGIERLRTRMVGPTHFIDALVEVPRTYPIDRIDEIKRRADDALKGAFGDSDLTF
HHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
TAIPVARDNETVRERIMVIARNSGLAVHHVTVHDLGCKITVSIDLEVDGDMPLIEAHDIA
EEEEECCCCHHHHHHEEEEECCCCEEEEEEEEEECCEEEEEEEEEEECCCCCCHHHHHHH
HELERHIRDDFGTDVEVDTHIEPLEPEMPHGYEAAPERVEEIRAALIGFAKNGAIHDIHS
HHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECHHE
VRVRDTEAGEIVNFHCYAEPSLSVIAVHDHVDEIERHMRRAFPSVKRVISHAEPPNAD
EEEECCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AIRFGRLLCSGSGCYTAPMSNFASTKSRVAAVSVLASASMAAAKFAVGIAIGSLALISE
CEEECCCEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALHSSIDLVATIITWFVVRVSDRPADDAHHYGHGKIESLSALGVIALLYVLAGGILVEAT
HHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHH
SRLREGAPPPAFSLWAFGILGVDILVNSWRAWALHRTAVATKSQALAADALHFASDVLGS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAVIAGLSLSALGFAWGDAAAAIGVAVMISILGLRLGRSTIQTLLDRAPEGVSDKARDVI
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHHHH
SAVPGVVGIERLRTRMVGPTHFIDALVEVPRTYPIDRIDEIKRRADDALKGAFGDSDLTF
HHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
TAIPVARDNETVRERIMVIARNSGLAVHHVTVHDLGCKITVSIDLEVDGDMPLIEAHDIA
EEEEECCCCHHHHHHEEEEECCCCEEEEEEEEEECCEEEEEEEEEEECCCCCCHHHHHHH
HELERHIRDDFGTDVEVDTHIEPLEPEMPHGYEAAPERVEEIRAALIGFAKNGAIHDIHS
HHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECHHE
VRVRDTEAGEIVNFHCYAEPSLSVIAVHDHVDEIERHMRRAFPSVKRVISHAEPPNAD
EEEECCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA