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The map label for this gene is 86750544

Identifier: 86750544

GI number: 86750544

Start: 3931836

End: 3932831

Strand: Direct

Name: 86750544

Synonym: RPB_3433

Alternate gene names: NA

Gene position: 3931836-3932831 (Clockwise)

Preceding gene: 86750543

Following gene: 86750545

Centisome position: 73.75

GC content: 66.16

Gene sequence:

>996_bases
ATGATCAAGACGCGATTCACCGAAACCTTCGGCATCGAGCATCCGATCGTCCAGGGCGGGATGCAATGGGTCGGCCGTGC
CGAACTGGTCGCGGCGGTCGCCAATGCCGGCGCGCTCGGCATGATCACCGCGCTGACGCAGCCGACGCCGGAAGACCTCA
CCAAGGAAATCGCGCGCTGCCGTGAGCTCACCGACAAGCCGTTCGGCGTGAACCTGACGATCCTGCCGGCGATCAAGCCG
CCGCCTTATGCGGAATATCGCCAGGCGATCATCGAGAGCGGCGTGCGGATCGTCGAAACCGCGGGCAACAAGCCGCAGGA
GCACGTCGAGGAATTCAAGAAGCACGGCGTCAAGGTGCTGCACAAATGCACCAGCGTCCGCCACGCGCTCTCGGCCGAGC
GGATGGGCGTCGACGGCATTTCGATCGACGGCTTCGAATGCGCCGGCCATCCGGGCGAGGACGACACCCCCGGCCTGATC
CTGATCCCGGCCGCCGCCGACAAGATCAAAATTCCGATGATCGCCTCCGGCGGCTTCGCCGACGGCCGCGGCCTGGTGGC
GGCGCTGTCGCTCGGCGCCGACGGCATCAACATGGGCACGCGCTTCATGTGCACCAAGGAGAGCCCGATCCACGAAGCGG
TGAAGCAGAAGATCGTCGACAATGACGAGCGCGCGACCGACCTGATCTTCCGGACCTTGCGCAACACCTCGCGCGTCGCG
AAGAACGCGATCAGCCAGCAGGTCCTCGAACTGGAAAAGCAGGGCGCGACCTTCGAGCAGGTCAAGGACCTGGTTGCGGG
CGCGCGCGGCAAGATGGTCTATGTCACCGGCGACACCGACGAAGGCGTGTGGTCGGCCGGCCAGGTGCAGGGGCTGATCC
ACGACATCCCGAGCTGCGCCGACCTGGTGTCGCGGATCGTGCGCGACGCCGAGGCGATCATTCGCGGCCGGCTCGAAACG
ATGCTATCGGGGGGCGCGCGTCAGGCTGCGGAATAG

Upstream 100 bases:

>100_bases
CCCCTTCCCCGCGCATTGGACATTTCGCTGCCGGTTCGGCAATCTGCCGCCGAACCACAACAGCAACAGGCGCGCGAGGC
GCCGGGTCCAGGGGCACATC

Downstream 100 bases:

>100_bases
AAGCGGCGGCCCGCGCGGCTCTGAAACGGGAAGCGTTGAGATGAAGGCCTATGTTTACGGCGCCGATGGCGCCGCCATCA
CCGAGATCGACAAGCCGGCG

Product: 2-nitropropane dioxygenase, NPD

Products: NA

Alternate protein names: Nitroalkane oxidase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIKTRFTETFGIEHPIVQGGMQWVGRAELVAAVANAGALGMITALTQPTPEDLTKEIARCRELTDKPFGVNLTILPAIKP
PPYAEYRQAIIESGVRIVETAGNKPQEHVEEFKKHGVKVLHKCTSVRHALSAERMGVDGISIDGFECAGHPGEDDTPGLI
LIPAAADKIKIPMIASGGFADGRGLVAALSLGADGINMGTRFMCTKESPIHEAVKQKIVDNDERATDLIFRTLRNTSRVA
KNAISQQVLELEKQGATFEQVKDLVAGARGKMVYVTGDTDEGVWSAGQVQGLIHDIPSCADLVSRIVRDAEAIIRGRLET
MLSGGARQAAE

Sequences:

>Translated_331_residues
MIKTRFTETFGIEHPIVQGGMQWVGRAELVAAVANAGALGMITALTQPTPEDLTKEIARCRELTDKPFGVNLTILPAIKP
PPYAEYRQAIIESGVRIVETAGNKPQEHVEEFKKHGVKVLHKCTSVRHALSAERMGVDGISIDGFECAGHPGEDDTPGLI
LIPAAADKIKIPMIASGGFADGRGLVAALSLGADGINMGTRFMCTKESPIHEAVKQKIVDNDERATDLIFRTLRNTSRVA
KNAISQQVLELEKQGATFEQVKDLVAGARGKMVYVTGDTDEGVWSAGQVQGLIHDIPSCADLVSRIVRDAEAIIRGRLET
MLSGGARQAAE
>Mature_331_residues
MIKTRFTETFGIEHPIVQGGMQWVGRAELVAAVANAGALGMITALTQPTPEDLTKEIARCRELTDKPFGVNLTILPAIKP
PPYAEYRQAIIESGVRIVETAGNKPQEHVEEFKKHGVKVLHKCTSVRHALSAERMGVDGISIDGFECAGHPGEDDTPGLI
LIPAAADKIKIPMIASGGFADGRGLVAALSLGADGINMGTRFMCTKESPIHEAVKQKIVDNDERATDLIFRTLRNTSRVA
KNAISQQVLELEKQGATFEQVKDLVAGARGKMVYVTGDTDEGVWSAGQVQGLIHDIPSCADLVSRIVRDAEAIIRGRLET
MLSGGARQAAE

Specific function: Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites [H]

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitronate monooxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR013785 [H]

Pfam domain/function: PF03060 NPD [H]

EC number: =1.13.12.16 [H]

Molecular weight: Translated: 35554; Mature: 35554

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTRFTETFGIEHPIVQGGMQWVGRAELVAAVANAGALGMITALTQPTPEDLTKEIARC
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
RELTDKPFGVNLTILPAIKPPPYAEYRQAIIESGVRIVETAGNKPQEHVEEFKKHGVKVL
HHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
HKCTSVRHALSAERMGVDGISIDGFECAGHPGEDDTPGLILIPAAADKIKIPMIASGGFA
HHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCC
DGRGLVAALSLGADGINMGTRFMCTKESPIHEAVKQKIVDNDERATDLIFRTLRNTSRVA
CCCHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KNAISQQVLELEKQGATFEQVKDLVAGARGKMVYVTGDTDEGVWSAGQVQGLIHDIPSCA
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHH
DLVSRIVRDAEAIIRGRLETMLSGGARQAAE
HHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MIKTRFTETFGIEHPIVQGGMQWVGRAELVAAVANAGALGMITALTQPTPEDLTKEIARC
CCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHH
RELTDKPFGVNLTILPAIKPPPYAEYRQAIIESGVRIVETAGNKPQEHVEEFKKHGVKVL
HHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHH
HKCTSVRHALSAERMGVDGISIDGFECAGHPGEDDTPGLILIPAAADKIKIPMIASGGFA
HHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCC
DGRGLVAALSLGADGINMGTRFMCTKESPIHEAVKQKIVDNDERATDLIFRTLRNTSRVA
CCCHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KNAISQQVLELEKQGATFEQVKDLVAGARGKMVYVTGDTDEGVWSAGQVQGLIHDIPSCA
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCHHH
DLVSRIVRDAEAIIRGRLETMLSGGARQAAE
HHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]