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The map label for this gene is torA [C]

Identifier: 86750250

GI number: 86750250

Start: 3588267

End: 3590636

Strand: Direct

Name: torA [C]

Synonym: RPB_3139

Alternate gene names: 86750250

Gene position: 3588267-3590636 (Clockwise)

Preceding gene: 86750235

Following gene: 86750252

Centisome position: 67.3

GC content: 68.14

Gene sequence:

>2370_bases
ATGACGGAGACCAACGACATGAACGACGGCTGGAGCACGCACAGTTCGCATTGGGGCGCCTTCTCCGCGCGCTGGGACGG
ACGAGCCCTCGAGGTCGAACCGGATGCGGGCGACCCGGCGCCCTCGCCCATCCTGGAAAATTTCACCGACGCGTTGCGAC
ACAAGGCCCGGATCGAACGGCCGATGGTTCGGCAAAGCTGGCTGGACAAAACCGGCGCCGAACGCACATTCGACGGCGCG
TTCGTCGAGTTGCCGTGGGACGAAGCGCTCGACCTTCTCGCCGCCGAACTGTCGCGCGTTCACGCCGGCTACGGCGCCGC
CGCGATCTTCGGCGGCTCCTATGGCTGGGCCAGCGCCGGCCGCTTTCATCACGCGCAGAGCCAGATCCACCGGTTTCTGA
ATGTTGCGTTCGGCGGTTACGTCCGCTCGGTCAACAGCTACAGCGCCGGCGCGTCCGCGGTGATCCTGCCGCATGTGCTG
GGGTCGTTCGAAGCGCTGTCGCGCCGCAACGTCACCTGGGAGCAGGTCAGCGAGCATACCGACACGGTGCTTGCATTCGG
CGGCATGGCGCTGAAAAATTCGATGGTGGCGAGCGGTGGCATCAGCCGGCATGTCGAACGCGATGCGATGCAGAAGGCCG
CACAGCGCGGCACCCGATTCTACTGCATCAGCCCGCTGCGCGACGATCTGCCCGAACAGGCATCGGCGACTTGGCTTCCG
ATCCGGGTCGGCACCGATGTGGCATTGATGCTCGGCCTCGCGCACAGCCTGGTCGCGGAGAATCTGCACGACCGCGACTT
CCTCGCGCGCTACTGCACTGGCTACGAGATCTTCGAGCGCTATCTGCAAGGCCTCGACGACGGTCAGCCGAAGCACGCCG
GCTGGGCGTCGGGTATCACCGGCATGGCCGCAGCGGAGATCGTCGCGCTCGCACGCCAGGCGGCCCGGGGCCGCACGCTG
GTGACGGTGTCGCATTCCCTGCAACGCGCGCAATACGGCGAGCAGCCGGTCTGGATGGGCGCGGTCCTCGCCGCGATGCT
CGGGCAGATCGGCCTGCCGGGCGGCGGCTACAACTACGCGCTCGGCGCGCTCGGCCACACCGGCCGGCAGGTCAATGCCG
TGCCGATTCCCACCATGCCGCAAGGGCGCAACGGCGTCGAAGAATTCATTCCGGTGGCGCGGATCAGCGACATGCTGCTC
CATCCCGGCCAGCCGTTCGAGTACAACGGCCGCACGCTCCGCTATCCGGACATCAAGCTGGTGTACTGGGCCGGCGGCAA
TCCGTTTCACCATCACCAGGACATCAACCGGCTGCGCCGTGCCTTCAACGTGCCGCAGACCATCGTGGTGCACGAGACCG
CGTGGACGGCGAGCGCACGCTTTGCCGACATCGTGCTGCCGGCGACGATGACACTCGAGCGCGACGACATCGGCGCCGCG
GCGACCGATCCGCGGCTGATCGCGATGCGCAAGGTCGCGCCTCCGGTCGGCGAAGCGCGGGACGATTTCGAGATCTTCGC
CGCGTTGGCACGGCGCCTCGGCGTCGAACAGGCTTACACCGAGGGCCGCGACAGCCGGCAATGGCTCGAGCATCTCTACG
AGACCACGCGCGCCGCGCTCGAAGCCAAGGGCTGGGACGCGCCGGCCTTCGACGAATTCTGGCGTCGTGGCGAACTGGCG
CTGCCGTCGGAGCCGGACGACGGCGGACTTTTGCGCGCGTTCCGCGACGACCCCGATGCGCACAAATTGCCGACGCCGAG
CGGCCTGATCGAGATCTTCTCGTCGACGATCGCGTCCTTCGACTACGCGGATTGTCCCGGCCACCCGACTTGGTTGCCGG
CGACCGATCAGCCGACCCCGCGGCATTCGTTGACGCTGATCGCCAATCAGCCGGCGACGCGCCTGCACAGCCAGCTCGAC
TTCGGTCGCTACAGCCAGTCGAGCAAGATCGCCGGCCGTGAGGTGGTGCGGCTCAACCCCGCCGATGCGCAATCGCGCGG
CATCGTCGCCGGCGACATCGTCCGCATCTTCAACGATCGCGGTGCCTGCCTGGCGGCCGCAGCGATCTCGGACGCCGCCG
CGGCCGGCGTGATCCACCTGCCGACCGGCGCGTGGTACGACCCGCTGCCGGACGGCGGCGACCGCCCGCTTTGCGTCCAC
GGCAATCCGAACGTGCTGACGCGCGACATCGGCACGTCGCGGCTGGCGCAAGGCTGCACCGGTCAGGTGACCGTCGTCGA
ATGTGAGAAATACGCGGGTCCCATTCCACCGATCCGCGCCTTCGATCCGTCGGCGCCGGTGAAGCGACGACGCGTCACTC
CGTCTCCGGCCGACGGCGACGGCGCCGCGACGGAGCGCCGCCAGGCATAA

Upstream 100 bases:

>100_bases
CGCACTGTCCGGCGCGACGGCACGACATCCCCTCGATTTTGAACGATTACAAACAAGGCGGCGTCGGCGCGGTTTCGTCC
GACAGAACCGCCGGGCACGA

Downstream 100 bases:

>100_bases
GCTGCCGCCGGCGCCTGCGCGCCGCTCGTCGCAGGGATCGACGACTCCGCCCGCCGCCGGCATCGATCGCGAAGCCGGCG
CGCCAGTCTACGTGTGCGTC

Product: trimethylamine-N-oxide reductase (cytochrome c)

Products: NAD+; trimethylamine; H2O [C]

Alternate protein names: BDS reductase; BSO reductase [H]

Number of amino acids: Translated: 789; Mature: 788

Protein sequence:

>789_residues
MTETNDMNDGWSTHSSHWGAFSARWDGRALEVEPDAGDPAPSPILENFTDALRHKARIERPMVRQSWLDKTGAERTFDGA
FVELPWDEALDLLAAELSRVHAGYGAAAIFGGSYGWASAGRFHHAQSQIHRFLNVAFGGYVRSVNSYSAGASAVILPHVL
GSFEALSRRNVTWEQVSEHTDTVLAFGGMALKNSMVASGGISRHVERDAMQKAAQRGTRFYCISPLRDDLPEQASATWLP
IRVGTDVALMLGLAHSLVAENLHDRDFLARYCTGYEIFERYLQGLDDGQPKHAGWASGITGMAAAEIVALARQAARGRTL
VTVSHSLQRAQYGEQPVWMGAVLAAMLGQIGLPGGGYNYALGALGHTGRQVNAVPIPTMPQGRNGVEEFIPVARISDMLL
HPGQPFEYNGRTLRYPDIKLVYWAGGNPFHHHQDINRLRRAFNVPQTIVVHETAWTASARFADIVLPATMTLERDDIGAA
ATDPRLIAMRKVAPPVGEARDDFEIFAALARRLGVEQAYTEGRDSRQWLEHLYETTRAALEAKGWDAPAFDEFWRRGELA
LPSEPDDGGLLRAFRDDPDAHKLPTPSGLIEIFSSTIASFDYADCPGHPTWLPATDQPTPRHSLTLIANQPATRLHSQLD
FGRYSQSSKIAGREVVRLNPADAQSRGIVAGDIVRIFNDRGACLAAAAISDAAAAGVIHLPTGAWYDPLPDGGDRPLCVH
GNPNVLTRDIGTSRLAQGCTGQVTVVECEKYAGPIPPIRAFDPSAPVKRRRVTPSPADGDGAATERRQA

Sequences:

>Translated_789_residues
MTETNDMNDGWSTHSSHWGAFSARWDGRALEVEPDAGDPAPSPILENFTDALRHKARIERPMVRQSWLDKTGAERTFDGA
FVELPWDEALDLLAAELSRVHAGYGAAAIFGGSYGWASAGRFHHAQSQIHRFLNVAFGGYVRSVNSYSAGASAVILPHVL
GSFEALSRRNVTWEQVSEHTDTVLAFGGMALKNSMVASGGISRHVERDAMQKAAQRGTRFYCISPLRDDLPEQASATWLP
IRVGTDVALMLGLAHSLVAENLHDRDFLARYCTGYEIFERYLQGLDDGQPKHAGWASGITGMAAAEIVALARQAARGRTL
VTVSHSLQRAQYGEQPVWMGAVLAAMLGQIGLPGGGYNYALGALGHTGRQVNAVPIPTMPQGRNGVEEFIPVARISDMLL
HPGQPFEYNGRTLRYPDIKLVYWAGGNPFHHHQDINRLRRAFNVPQTIVVHETAWTASARFADIVLPATMTLERDDIGAA
ATDPRLIAMRKVAPPVGEARDDFEIFAALARRLGVEQAYTEGRDSRQWLEHLYETTRAALEAKGWDAPAFDEFWRRGELA
LPSEPDDGGLLRAFRDDPDAHKLPTPSGLIEIFSSTIASFDYADCPGHPTWLPATDQPTPRHSLTLIANQPATRLHSQLD
FGRYSQSSKIAGREVVRLNPADAQSRGIVAGDIVRIFNDRGACLAAAAISDAAAAGVIHLPTGAWYDPLPDGGDRPLCVH
GNPNVLTRDIGTSRLAQGCTGQVTVVECEKYAGPIPPIRAFDPSAPVKRRRVTPSPADGDGAATERRQA
>Mature_788_residues
TETNDMNDGWSTHSSHWGAFSARWDGRALEVEPDAGDPAPSPILENFTDALRHKARIERPMVRQSWLDKTGAERTFDGAF
VELPWDEALDLLAAELSRVHAGYGAAAIFGGSYGWASAGRFHHAQSQIHRFLNVAFGGYVRSVNSYSAGASAVILPHVLG
SFEALSRRNVTWEQVSEHTDTVLAFGGMALKNSMVASGGISRHVERDAMQKAAQRGTRFYCISPLRDDLPEQASATWLPI
RVGTDVALMLGLAHSLVAENLHDRDFLARYCTGYEIFERYLQGLDDGQPKHAGWASGITGMAAAEIVALARQAARGRTLV
TVSHSLQRAQYGEQPVWMGAVLAAMLGQIGLPGGGYNYALGALGHTGRQVNAVPIPTMPQGRNGVEEFIPVARISDMLLH
PGQPFEYNGRTLRYPDIKLVYWAGGNPFHHHQDINRLRRAFNVPQTIVVHETAWTASARFADIVLPATMTLERDDIGAAA
TDPRLIAMRKVAPPVGEARDDFEIFAALARRLGVEQAYTEGRDSRQWLEHLYETTRAALEAKGWDAPAFDEFWRRGELAL
PSEPDDGGLLRAFRDDPDAHKLPTPSGLIEIFSSTIASFDYADCPGHPTWLPATDQPTPRHSLTLIANQPATRLHSQLDF
GRYSQSSKIAGREVVRLNPADAQSRGIVAGDIVRIFNDRGACLAAAAISDAAAAGVIHLPTGAWYDPLPDGGDRPLCVHG
NPNVLTRDIGTSRLAQGCTGQVTVVECEKYAGPIPPIRAFDPSAPVKRRRVTPSPADGDGAATERRQA

Specific function: This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source. It reduces a spontaneous oxidation product of biotin, D-biotin D-sulfoxide (BSO or BDS), back to biotin [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787231, Length=777, Percent_Identity=40.9266409266409, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI87081994, Length=773, Percent_Identity=39.1979301423027, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI145693196, Length=771, Percent_Identity=39.6887159533074, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1787870, Length=750, Percent_Identity=31.4666666666667, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI87081797, Length=718, Percent_Identity=30.2228412256267, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI171474008, Length=741, Percent_Identity=28.3400809716599, Blast_Score=215, Evalue=1e-56,
Organism=Escherichia coli, GI3868721, Length=670, Percent_Identity=21.4925373134328, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006658
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006655 [H]

Pfam domain/function: PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.7.2.3 [C]

Molecular weight: Translated: 85901; Mature: 85770

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETNDMNDGWSTHSSHWGAFSARWDGRALEVEPDAGDPAPSPILENFTDALRHKARIER
CCCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
PMVRQSWLDKTGAERTFDGAFVELPWDEALDLLAAELSRVHAGYGAAAIFGGSYGWASAG
HHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
RFHHAQSQIHRFLNVAFGGYVRSVNSYSAGASAVILPHVLGSFEALSRRNVTWEQVSEHT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DTVLAFGGMALKNSMVASGGISRHVERDAMQKAAQRGTRFYCISPLRDDLPEQASATWLP
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHCCCCEEE
IRVGTDVALMLGLAHSLVAENLHDRDFLARYCTGYEIFERYLQGLDDGQPKHAGWASGIT
EEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCH
GMAAAEIVALARQAARGRTLVTVSHSLQRAQYGEQPVWMGAVLAAMLGQIGLPGGGYNYA
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
LGALGHTGRQVNAVPIPTMPQGRNGVEEFIPVARISDMLLHPGQPFEYNGRTLRYPDIKL
HHHHCCCCCEECEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEE
VYWAGGNPFHHHQDINRLRRAFNVPQTIVVHETAWTASARFADIVLPATMTLERDDIGAA
EEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCEEEEECCCCCCC
ATDPRLIAMRKVAPPVGEARDDFEIFAALARRLGVEQAYTEGRDSRQWLEHLYETTRAAL
CCCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHH
EAKGWDAPAFDEFWRRGELALPSEPDDGGLLRAFRDDPDAHKLPTPSGLIEIFSSTIASF
HCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEHCCCCCCCCCCCCCHHHHHHHHHHHHCC
DYADCPGHPTWLPATDQPTPRHSLTLIANQPATRLHSQLDFGRYSQSSKIAGREVVRLNP
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEEEECC
ADAQSRGIVAGDIVRIFNDRGACLAAAAISDAAAAGVIHLPTGAWYDPLPDGGDRPLCVH
CCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEE
GNPNVLTRDIGTSRLAQGCTGQVTVVECEKYAGPIPPIRAFDPSAPVKRRRVTPSPADGD
CCCCEEEECCCHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
GAATERRQA
CCCCCCCCC
>Mature Secondary Structure 
TETNDMNDGWSTHSSHWGAFSARWDGRALEVEPDAGDPAPSPILENFTDALRHKARIER
CCCCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
PMVRQSWLDKTGAERTFDGAFVELPWDEALDLLAAELSRVHAGYGAAAIFGGSYGWASAG
HHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
RFHHAQSQIHRFLNVAFGGYVRSVNSYSAGASAVILPHVLGSFEALSRRNVTWEQVSEHT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHH
DTVLAFGGMALKNSMVASGGISRHVERDAMQKAAQRGTRFYCISPLRDDLPEQASATWLP
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCHHCCCCEEE
IRVGTDVALMLGLAHSLVAENLHDRDFLARYCTGYEIFERYLQGLDDGQPKHAGWASGIT
EEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCHHCCCH
GMAAAEIVALARQAARGRTLVTVSHSLQRAQYGEQPVWMGAVLAAMLGQIGLPGGGYNYA
HHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
LGALGHTGRQVNAVPIPTMPQGRNGVEEFIPVARISDMLLHPGQPFEYNGRTLRYPDIKL
HHHHCCCCCEECEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCEEE
VYWAGGNPFHHHQDINRLRRAFNVPQTIVVHETAWTASARFADIVLPATMTLERDDIGAA
EEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEECCEEEEECCCCCCC
ATDPRLIAMRKVAPPVGEARDDFEIFAALARRLGVEQAYTEGRDSRQWLEHLYETTRAAL
CCCCCEEEHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHHHHHHHH
EAKGWDAPAFDEFWRRGELALPSEPDDGGLLRAFRDDPDAHKLPTPSGLIEIFSSTIASF
HCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEHCCCCCCCCCCCCCHHHHHHHHHHHHCC
DYADCPGHPTWLPATDQPTPRHSLTLIANQPATRLHSQLDFGRYSQSSKIAGREVVRLNP
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCEEEEECC
ADAQSRGIVAGDIVRIFNDRGACLAAAAISDAAAAGVIHLPTGAWYDPLPDGGDRPLCVH
CCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCEEEE
GNPNVLTRDIGTSRLAQGCTGQVTVVECEKYAGPIPPIRAFDPSAPVKRRRVTPSPADGD
CCCCEEEECCCHHHHHCCCCCCEEEEEEHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
GAATERRQA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron; Molybdenum; Zinc [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.33 {benzyl} 0.95 {trimethylamine-N-oxide}} 0.67 {trimethylamine-N-oxide}} 0.15 {methyl} [C]

Substrates: NADH; trimethylamine-N-oxide [C]

Specific reaction: NADH + trimethylamine-N-oxide = NAD+ + trimethylamine + H2O [C]

General reaction: Redox reaction [C]

Inhibitor: EDTA; KCN; N-Ethyl maleimide; NaN3 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7733660 [H]