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The map label for this gene is liaS [H]

Identifier: 86750180

GI number: 86750180

Start: 3496354

End: 3498759

Strand: Direct

Name: liaS [H]

Synonym: RPB_3064

Alternate gene names: 86750180

Gene position: 3496354-3498759 (Clockwise)

Preceding gene: 86750179

Following gene: 86750181

Centisome position: 65.58

GC content: 62.3

Gene sequence:

>2406_bases
ATGACGATCTTCTTCTGGAATCCGATTTCGCTGACCTATCTGACCCAGCTGGTCCTGGTGCTGGTGATCGCGGCCCTGCT
CGTCAAGACGCAGCTCAGGCGAGATTCGCACGCCGAGACGCCACGTTCGACCATCCTGCTGGCGAGCAGCTTTGGCTTTT
ATGGCAGCTACAGCGTCTTGAGTTTCCTTCGGGCGATACTGCGCACGGACCTCCAAGCCTGGGTCGAACCACCGATCAGC
GTCCTGTCGTCCATCGCGATCGTGCTCTTCGTCCAATTCGCTCATCACTTCCCAGAACCGTTCGCGCGCGAGCGGGTCGA
GCGCCGCATCGTAACGGCTCTGTCCATTGTCTTCGTCGCCGTCGAGGTCGGCTTCGCGATCTATCGCTACCAAGAACTCG
GCCTCGGGCATGTCGAGTACCGGCCGGCGTTCATGGATCTGCCCGCCTTCGCGGCGATGGGTTGGCTGTTCGTACTGGCT
TTTCGTCGGGCTCTTCGCGCTGCGACGGATCGCGATCGAAGCTGTCCACCGGACGCGCCGAAGCGCGAATGGTCGAACAA
TCCGGCCGCTGCCGCCCGCGCGGTGCTGCTGTTCGCCATCGTGCCTTTGCTGTTGGTGATCGTCACCCTGTTGCAGGCCT
ATCACCTGATCGGTGTCGAGACGCGGGAGGTTGCCGCCGCCTTGCTGAACCTCGCGGGGCTTCTCGGTTTCCTTCTTCTC
TATTTGAACTACTTCGTGACCGAAGGGTCGTTCTCGATCAAACTGAGCAGCACCGCGCTCGCTCTCGTTCTGGGAGGCTT
CACCAGCGTCGCCTGGATCATCGGCTTCGACTTTTCCGGCGCCTACAAGGGCGACACTTCCAGACTCAGATATCAGACCC
ACGTCTTCCGCCCCACGGCCGAGGGCGGCTACAACGTTTCTCGCGTCGACTATGCGTTCGTCCCGGTCGAGGCACAGGTC
CGCCAGCCCGGAGCCTTCCGAATCGAGTTGCCGTTCGACTTTCCGTTCTTTTCGAAAGTCCACCGCGAGGCCTTCATCCG
CGAGGACGGCATGGTGGGATTCGACTCCGTCCCAACCTGGCCCGACACGGTGTTCCGGAATGGCGCGCAACCGGCGATCT
TTCCACTAGCGGTCGATCTCGCCGAGCCGGCGTCGAACGTGTCCGCGGAGGAGGGAATTCACCTGTCCACCTCTCCGGAG
GAGGTGGTCGTCACATGGTCAGGCTGGTCCCGCGCCGGGGAGACGGGCGATCACTACGTGTTCCAAATGATCTTGCGGCC
ATCGGGCGAGATCCAATTCTCCTATCAGGAGCTACCGCGGATTCTGCTCAACGATCTGTTCATGCCCAACCGGGTGCCGA
TGCTCATGGGAGTGACCCCTGGATTCTCCAACGGCGAGACGCGGCTCGTACACTTCCAGCGGGGACAACCCTTGCACGGA
CCGCCAGGGACCGGCCTGATCGAGAACTTTCGGTTGGACTACCTCGCCTATCTGAACGGGATCTACACCCCTCTCGCCTG
GCTGATCCTGGTCACCTCGCCGGCAACGTTGCTGGTGTTTCAAGCTTTGTTCCGGACCAACCTCGTGCGTCCGCTCGACG
CGCTGCTCGCCGGCGTCGGACGGTTTCGCGAGGGGACGCTCTCCCGGCGCCTGCCGATCCACTTCCCCGACGAGATCGGC
TATCTGACTCGATCCTTCAACGAGATGGCCGAGAAGCAGAACGATCTGCTGAACGGTCTGGAAGACCGCGTCACGGAACG
CACCGCGGAGGTCACGATCCTGGCCGACCGCAATGCCCGGCTCGAGGAGCGCAGCCGGATTTCGAGCGACCTCCACGACG
CCGTCAGCCAGACCCTGTTTTCTGCAGTGCTGATTGCCGAAACCCTGCCCGAGCAGTGGAAGCGTGACAGGACCAAGGCC
GAGGCTCTGCTTACCGACCTCGCCCGGCTGAACCGTTCGGCACTTGATGAGATGCGCGACCTCCTGAGCCGCGTCCGGTC
GCGGGATCTCATCGACCCTGTCCTCGGGACGCTCCTTCGCGGCCTCGTCGCGCGGTTCGAGGAACGCCACGCTCTCGGAA
TCAGCTTGACGGTAGGCAGCGACGCTCGCCTGGACGAAGACGTTCAGATCGCCTTCTTTCGGATCGCACAGGAAGGACTG
AACAACGTCATGAAACACGCCTCCTCCTCGACCGTGCGGCTGTTCTACGAGGCGCTCGAAGGTCAGGCAATCCTGACGAT
CGAGGACGACGGCAACGGATTCGACACCGCTCACCTTCGCGAAGGCAGCATGGGCCTGCAGATCATGGCGGAGCGGGCAA
GAAAGATCGGCGCCGTTCTCGAGACGACGTCGAGTCCGGGCCACGGGACGCAGATCACGCTCATCTGGAATCAAAACGAT
GCCTGA

Upstream 100 bases:

>100_bases
TAGAACGAGCCGACCGTTCGGACCGACGAACAGCGCCCTCCCGGATCTTCGGCTCTCGACCACTCCAGGCGCAAGGCGGC
TCCGTGACGGATTCCAGACC

Downstream 100 bases:

>100_bases
GACCTCGATCCGCCTGCTGATCGCCGACGATCATCCGGTCGTAAGGGCCGGCCTGCGCCTCGTGCTCGGCACCATTTCCG
GATTGTCCGTCGTCGCGGAG

Product: putative signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 801; Mature: 800

Protein sequence:

>801_residues
MTIFFWNPISLTYLTQLVLVLVIAALLVKTQLRRDSHAETPRSTILLASSFGFYGSYSVLSFLRAILRTDLQAWVEPPIS
VLSSIAIVLFVQFAHHFPEPFARERVERRIVTALSIVFVAVEVGFAIYRYQELGLGHVEYRPAFMDLPAFAAMGWLFVLA
FRRALRAATDRDRSCPPDAPKREWSNNPAAAARAVLLFAIVPLLLVIVTLLQAYHLIGVETREVAAALLNLAGLLGFLLL
YLNYFVTEGSFSIKLSSTALALVLGGFTSVAWIIGFDFSGAYKGDTSRLRYQTHVFRPTAEGGYNVSRVDYAFVPVEAQV
RQPGAFRIELPFDFPFFSKVHREAFIREDGMVGFDSVPTWPDTVFRNGAQPAIFPLAVDLAEPASNVSAEEGIHLSTSPE
EVVVTWSGWSRAGETGDHYVFQMILRPSGEIQFSYQELPRILLNDLFMPNRVPMLMGVTPGFSNGETRLVHFQRGQPLHG
PPGTGLIENFRLDYLAYLNGIYTPLAWLILVTSPATLLVFQALFRTNLVRPLDALLAGVGRFREGTLSRRLPIHFPDEIG
YLTRSFNEMAEKQNDLLNGLEDRVTERTAEVTILADRNARLEERSRISSDLHDAVSQTLFSAVLIAETLPEQWKRDRTKA
EALLTDLARLNRSALDEMRDLLSRVRSRDLIDPVLGTLLRGLVARFEERHALGISLTVGSDARLDEDVQIAFFRIAQEGL
NNVMKHASSSTVRLFYEALEGQAILTIEDDGNGFDTAHLREGSMGLQIMAERARKIGAVLETTSSPGHGTQITLIWNQND
A

Sequences:

>Translated_801_residues
MTIFFWNPISLTYLTQLVLVLVIAALLVKTQLRRDSHAETPRSTILLASSFGFYGSYSVLSFLRAILRTDLQAWVEPPIS
VLSSIAIVLFVQFAHHFPEPFARERVERRIVTALSIVFVAVEVGFAIYRYQELGLGHVEYRPAFMDLPAFAAMGWLFVLA
FRRALRAATDRDRSCPPDAPKREWSNNPAAAARAVLLFAIVPLLLVIVTLLQAYHLIGVETREVAAALLNLAGLLGFLLL
YLNYFVTEGSFSIKLSSTALALVLGGFTSVAWIIGFDFSGAYKGDTSRLRYQTHVFRPTAEGGYNVSRVDYAFVPVEAQV
RQPGAFRIELPFDFPFFSKVHREAFIREDGMVGFDSVPTWPDTVFRNGAQPAIFPLAVDLAEPASNVSAEEGIHLSTSPE
EVVVTWSGWSRAGETGDHYVFQMILRPSGEIQFSYQELPRILLNDLFMPNRVPMLMGVTPGFSNGETRLVHFQRGQPLHG
PPGTGLIENFRLDYLAYLNGIYTPLAWLILVTSPATLLVFQALFRTNLVRPLDALLAGVGRFREGTLSRRLPIHFPDEIG
YLTRSFNEMAEKQNDLLNGLEDRVTERTAEVTILADRNARLEERSRISSDLHDAVSQTLFSAVLIAETLPEQWKRDRTKA
EALLTDLARLNRSALDEMRDLLSRVRSRDLIDPVLGTLLRGLVARFEERHALGISLTVGSDARLDEDVQIAFFRIAQEGL
NNVMKHASSSTVRLFYEALEGQAILTIEDDGNGFDTAHLREGSMGLQIMAERARKIGAVLETTSSPGHGTQITLIWNQND
A
>Mature_800_residues
TIFFWNPISLTYLTQLVLVLVIAALLVKTQLRRDSHAETPRSTILLASSFGFYGSYSVLSFLRAILRTDLQAWVEPPISV
LSSIAIVLFVQFAHHFPEPFARERVERRIVTALSIVFVAVEVGFAIYRYQELGLGHVEYRPAFMDLPAFAAMGWLFVLAF
RRALRAATDRDRSCPPDAPKREWSNNPAAAARAVLLFAIVPLLLVIVTLLQAYHLIGVETREVAAALLNLAGLLGFLLLY
LNYFVTEGSFSIKLSSTALALVLGGFTSVAWIIGFDFSGAYKGDTSRLRYQTHVFRPTAEGGYNVSRVDYAFVPVEAQVR
QPGAFRIELPFDFPFFSKVHREAFIREDGMVGFDSVPTWPDTVFRNGAQPAIFPLAVDLAEPASNVSAEEGIHLSTSPEE
VVVTWSGWSRAGETGDHYVFQMILRPSGEIQFSYQELPRILLNDLFMPNRVPMLMGVTPGFSNGETRLVHFQRGQPLHGP
PGTGLIENFRLDYLAYLNGIYTPLAWLILVTSPATLLVFQALFRTNLVRPLDALLAGVGRFREGTLSRRLPIHFPDEIGY
LTRSFNEMAEKQNDLLNGLEDRVTERTAEVTILADRNARLEERSRISSDLHDAVSQTLFSAVLIAETLPEQWKRDRTKAE
ALLTDLARLNRSALDEMRDLLSRVRSRDLIDPVLGTLLRGLVARFEERHALGISLTVGSDARLDEDVQIAFFRIAQEGLN
NVMKHASSSTVRLFYEALEGQAILTIEDDGNGFDTAHLREGSMGLQIMAERARKIGAVLETTSSPGHGTQITLIWNQNDA

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG4585

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788812, Length=204, Percent_Identity=28.4313725490196, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082311, Length=222, Percent_Identity=30.1801801801802, Blast_Score=67, Evalue=7e-12,
Organism=Escherichia coli, GI1787474, Length=216, Percent_Identity=24.537037037037, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR011712
- InterPro:   IPR005467
- InterPro:   IPR017202 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 89390; Mature: 89259

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS50885 HAMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIFFWNPISLTYLTQLVLVLVIAALLVKTQLRRDSHAETPRSTILLASSFGFYGSYSVL
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHH
SFLRAILRTDLQAWVEPPISVLSSIAIVLFVQFAHHFPEPFARERVERRIVTALSIVFVA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VEVGFAIYRYQELGLGHVEYRPAFMDLPAFAAMGWLFVLAFRRALRAATDRDRSCPPDAP
HHHHHHHHHHHHCCCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KREWSNNPAAAARAVLLFAIVPLLLVIVTLLQAYHLIGVETREVAAALLNLAGLLGFLLL
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YLNYFVTEGSFSIKLSSTALALVLGGFTSVAWIIGFDFSGAYKGDTSRLRYQTHVFRPTA
HHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHEEEEEEEECCCC
EGGYNVSRVDYAFVPVEAQVRQPGAFRIELPFDFPFFSKVHREAFIREDGMVGFDSVPTW
CCCCCEEEEEEEEECCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PDTVFRNGAQPAIFPLAVDLAEPASNVSAEEGIHLSTSPEEVVVTWSGWSRAGETGDHYV
CHHHHCCCCCCCEEEEEEEHHCCCCCCCHHCCEEECCCCCEEEEEECCCCCCCCCCCHHH
FQMILRPSGEIQFSYQELPRILLNDLFMPNRVPMLMGVTPGFSNGETRLVHFQRGQPLHG
HHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCC
PPGTGLIENFRLDYLAYLNGIYTPLAWLILVTSPATLLVFQALFRTNLVRPLDALLAGVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RFREGTLSRRLPIHFPDEIGYLTRSFNEMAEKQNDLLNGLEDRVTERTAEVTILADRNAR
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCC
LEERSRISSDLHDAVSQTLFSAVLIAETLPEQWKRDRTKAEALLTDLARLNRSALDEMRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSRVRSRDLIDPVLGTLLRGLVARFEERHALGISLTVGSDARLDEDVQIAFFRIAQEGL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
NNVMKHASSSTVRLFYEALEGQAILTIEDDGNGFDTAHLREGSMGLQIMAERARKIGAVL
HHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
ETTSSPGHGTQITLIWNQNDA
CCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure 
TIFFWNPISLTYLTQLVLVLVIAALLVKTQLRRDSHAETPRSTILLASSFGFYGSYSVL
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHH
SFLRAILRTDLQAWVEPPISVLSSIAIVLFVQFAHHFPEPFARERVERRIVTALSIVFVA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
VEVGFAIYRYQELGLGHVEYRPAFMDLPAFAAMGWLFVLAFRRALRAATDRDRSCPPDAP
HHHHHHHHHHHHCCCCCEECCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
KREWSNNPAAAARAVLLFAIVPLLLVIVTLLQAYHLIGVETREVAAALLNLAGLLGFLLL
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YLNYFVTEGSFSIKLSSTALALVLGGFTSVAWIIGFDFSGAYKGDTSRLRYQTHVFRPTA
HHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHEEEEEEEECCCC
EGGYNVSRVDYAFVPVEAQVRQPGAFRIELPFDFPFFSKVHREAFIREDGMVGFDSVPTW
CCCCCEEEEEEEEECCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
PDTVFRNGAQPAIFPLAVDLAEPASNVSAEEGIHLSTSPEEVVVTWSGWSRAGETGDHYV
CHHHHCCCCCCCEEEEEEEHHCCCCCCCHHCCEEECCCCCEEEEEECCCCCCCCCCCHHH
FQMILRPSGEIQFSYQELPRILLNDLFMPNRVPMLMGVTPGFSNGETRLVHFQRGQPLHG
HHHHHCCCCCEEECHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCC
PPGTGLIENFRLDYLAYLNGIYTPLAWLILVTSPATLLVFQALFRTNLVRPLDALLAGVG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
RFREGTLSRRLPIHFPDEIGYLTRSFNEMAEKQNDLLNGLEDRVTERTAEVTILADRNAR
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCC
LEERSRISSDLHDAVSQTLFSAVLIAETLPEQWKRDRTKAEALLTDLARLNRSALDEMRD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLSRVRSRDLIDPVLGTLLRGLVARFEERHALGISLTVGSDARLDEDVQIAFFRIAQEGL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
NNVMKHASSSTVRLFYEALEGQAILTIEDDGNGFDTAHLREGSMGLQIMAERARKIGAVL
HHHHHHCCCHHHHHHHHHHCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHH
ETTSSPGHGTQITLIWNQNDA
CCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]