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The map label for this gene is vraR [H]

Identifier: 86750181

GI number: 86750181

Start: 3498752

End: 3499405

Strand: Direct

Name: vraR [H]

Synonym: RPB_3065

Alternate gene names: 86750181

Gene position: 3498752-3499405 (Clockwise)

Preceding gene: 86750180

Following gene: 86750182

Centisome position: 65.62

GC content: 64.07

Gene sequence:

>654_bases
ATGCCTGAGACCTCGATCCGCCTGCTGATCGCCGACGATCATCCGGTCGTAAGGGCCGGCCTGCGCCTCGTGCTCGGCAC
CATTTCCGGATTGTCCGTCGTCGCGGAGGCGGAATCTGGAGAGGAAGCCCTCTACTTGTGCTCAAAGCACCGTCCAGACA
TCGTTATCATGGACGTGAAGATGGCCGGCATCGGAGGCACTTCATCCACCCGCGCGATCTGCCAGACCTATCACCCCGGC
ACCCGGGTTCTCGGTCTCACGACCGAGGCGGATACGACCACGGTGTCCCAGATGATCGACGCGGGGGCGAGCGGGCTGCT
GCTCAAGACCTGCACCGTGAACGAACTCGAGACCGCGATCCGCGACGTCTTGGCGGGCCGCATCGTCCTCGACCCCCTGC
TGCAGAAGGGCGCACCCCGGGCACGAACGGCGGCAGCGGAAAGCGAGATCACCCTCGGCAGCCAGCAGAAGAAGGTGCTG
GCGCTACTCGTCAAGGGCTTCACCAATTCCGAGATCGCCGAGCGGCTCGGCATCTCGCTGCCGACCGCGCGCTATCACGT
CAGCGCAATTCTGGTGAAGTTGGGAGCCTCGAACCGAGCCGAAGCAGCGGGATTGGCGGTCCGCTTGAACATCGTTGGGG
CCGACGATTATTGA

Upstream 100 bases:

>100_bases
CAGATCATGGCGGAGCGGGCAAGAAAGATCGGCGCCGTTCTCGAGACGACGTCGAGTCCGGGCCACGGGACGCAGATCAC
GCTCATCTGGAATCAAAACG

Downstream 100 bases:

>100_bases
CCCGCTTGGGGGCTGGCGGATGAGGGCCGCTCGACGCCCGTCGCCTGCTCCCGACGCTTACCGCGAGGCCGTCGCCAGCC
TCGCCGATGAGGCCCTCTAT

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MPETSIRLLIADDHPVVRAGLRLVLGTISGLSVVAEAESGEEALYLCSKHRPDIVIMDVKMAGIGGTSSTRAICQTYHPG
TRVLGLTTEADTTTVSQMIDAGASGLLLKTCTVNELETAIRDVLAGRIVLDPLLQKGAPRARTAAAESEITLGSQQKKVL
ALLVKGFTNSEIAERLGISLPTARYHVSAILVKLGASNRAEAAGLAVRLNIVGADDY

Sequences:

>Translated_217_residues
MPETSIRLLIADDHPVVRAGLRLVLGTISGLSVVAEAESGEEALYLCSKHRPDIVIMDVKMAGIGGTSSTRAICQTYHPG
TRVLGLTTEADTTTVSQMIDAGASGLLLKTCTVNELETAIRDVLAGRIVLDPLLQKGAPRARTAAAESEITLGSQQKKVL
ALLVKGFTNSEIAERLGISLPTARYHVSAILVKLGASNRAEAAGLAVRLNIVGADDY
>Mature_216_residues
PETSIRLLIADDHPVVRAGLRLVLGTISGLSVVAEAESGEEALYLCSKHRPDIVIMDVKMAGIGGTSSTRAICQTYHPGT
RVLGLTTEADTTTVSQMIDAGASGLLLKTCTVNELETAIRDVLAGRIVLDPLLQKGAPRARTAAAESEITLGSQQKKVLA
LLVKGFTNSEIAERLGISLPTARYHVSAILVKLGASNRAEAAGLAVRLNIVGADDY

Specific function: Member of the two-component regulatory system vraS/vraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu3, which leads to vancomycin resistance [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=213, Percent_Identity=31.924882629108, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=32.6829268292683, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790102, Length=198, Percent_Identity=31.8181818181818, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI1786747, Length=209, Percent_Identity=28.2296650717703, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787473, Length=201, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=25.8706467661692, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788712, Length=203, Percent_Identity=25.615763546798, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22799; Mature: 22667

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETSIRLLIADDHPVVRAGLRLVLGTISGLSVVAEAESGEEALYLCSKHRPDIVIMDVK
CCCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCEEEEEEE
MAGIGGTSSTRAICQTYHPGTRVLGLTTEADTTTVSQMIDAGASGLLLKTCTVNELETAI
EECCCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHH
RDVLAGRIVLDPLLQKGAPRARTAAAESEITLGSQQKKVLALLVKGFTNSEIAERLGISL
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHCCCC
PTARYHVSAILVKLGASNRAEAAGLAVRLNIVGADDY
CCHHHHHHHHEEECCCCCCCCCCCEEEEEEEEECCCC
>Mature Secondary Structure 
PETSIRLLIADDHPVVRAGLRLVLGTISGLSVVAEAESGEEALYLCSKHRPDIVIMDVK
CCCEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCCCEEEEEEE
MAGIGGTSSTRAICQTYHPGTRVLGLTTEADTTTVSQMIDAGASGLLLKTCTVNELETAI
EECCCCCCCCHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCEEEEEECHHHHHHHH
RDVLAGRIVLDPLLQKGAPRARTAAAESEITLGSQQKKVLALLVKGFTNSEIAERLGISL
HHHHHHHHHHHHHHHCCCCCHHHHHCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHCCCC
PTARYHVSAILVKLGASNRAEAAGLAVRLNIVGADDY
CCHHHHHHHHEEECCCCCCCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]