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The map label for this gene is pdxJ

Identifier: 86749727

GI number: 86749727

Start: 2993666

End: 2994430

Strand: Direct

Name: pdxJ

Synonym: RPB_2609

Alternate gene names: 86749727

Gene position: 2993666-2994430 (Clockwise)

Preceding gene: 86749726

Following gene: 86749728

Centisome position: 56.15

GC content: 68.37

Gene sequence:

>765_bases
ATGCCGAAATCCTCTCCTCTGCGTCTTGGGGTCAACATCGATCACATCGCCACGGTCCGCAATGCGCGCGGCGGTCGCCA
TCCCGATCCGGTGCGCGCGGCGCTGACCGCGATCGAGGCCGGCGCCGACGGCATCACGGCGCATCTGCGCGAGGATCGCC
GCCACATTCGCGACAACGACATGGAGCGGTTGAAGGCGGAGATTTCCAAGCCGCTGAATTTCGAGATGGCGGCGACGCCG
GACATGGTCCGGATCGCGCTCGGCGTGAAGCCTCACGCCGTCTGCCTGGTTCCGGAGCGGCGCGAAGAACTCACCACCGA
GGGCGGCCTCGATGTCGTCGGCCAGCGCGACTCGCTGGCGCCGTCGATCGCGCGATTCAACGATGCCGGCATCCGGGTGT
CGCTGTTCATCGCCGCCGATCCGGCACAGATCGAGATGGCCGCCAAACTGAAGGCCCCGGCGATCGAACTGCACACCGGC
GCCTGGTGCGACGCCATCACCGACGACGAACCGGCCAAGGTCGCCGAGGAGTGGCGGCGAATCGTCGCCGGCGCCGCGCT
GGCGCAGTCGGCCGGGCTCGAGGTCCACGCCGGTCACGGCCTCGACTACGCCACCGCCGAGACGATCGCGGCGCTGCCGC
AGATCGTGGAGCTCAATATCGGCTTCTACATGATCGGCGAGGCGCTGTTCGTCGGCCTCGGCGAAACGGTGAAGGCGATG
CGGTCGGCGATGGACCGTGGCCGTGCCAGGGTGATCGCGGCATGA

Upstream 100 bases:

>100_bases
TCGCCCAGCTTCGCGCCAAGGCGGTCGTGGCCCACGTCGAGCGCGTCAATGGATAGCGCGGCGCCGCAAGTCCTTCCGTA
GTACGTCCCGCAGGTTCAAC

Downstream 100 bases:

>100_bases
TCATCGGAATCGGCTCCGACCTGATCGACATTACCCGGATCGCCAAGGTGATCGACCGGCATGGGGAACGGTTTCTCGAC
CGCATCTTCACCGAAACCGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MPKSSPLRLGVNIDHIATVRNARGGRHPDPVRAALTAIEAGADGITAHLREDRRHIRDNDMERLKAEISKPLNFEMAATP
DMVRIALGVKPHAVCLVPERREELTTEGGLDVVGQRDSLAPSIARFNDAGIRVSLFIAADPAQIEMAAKLKAPAIELHTG
AWCDAITDDEPAKVAEEWRRIVAGAALAQSAGLEVHAGHGLDYATAETIAALPQIVELNIGFYMIGEALFVGLGETVKAM
RSAMDRGRARVIAA

Sequences:

>Translated_254_residues
MPKSSPLRLGVNIDHIATVRNARGGRHPDPVRAALTAIEAGADGITAHLREDRRHIRDNDMERLKAEISKPLNFEMAATP
DMVRIALGVKPHAVCLVPERREELTTEGGLDVVGQRDSLAPSIARFNDAGIRVSLFIAADPAQIEMAAKLKAPAIELHTG
AWCDAITDDEPAKVAEEWRRIVAGAALAQSAGLEVHAGHGLDYATAETIAALPQIVELNIGFYMIGEALFVGLGETVKAM
RSAMDRGRARVIAA
>Mature_253_residues
PKSSPLRLGVNIDHIATVRNARGGRHPDPVRAALTAIEAGADGITAHLREDRRHIRDNDMERLKAEISKPLNFEMAATPD
MVRIALGVKPHAVCLVPERREELTTEGGLDVVGQRDSLAPSIARFNDAGIRVSLFIAADPAQIEMAAKLKAPAIELHTGA
WCDAITDDEPAKVAEEWRRIVAGAALAQSAGLEVHAGHGLDYATAETIAALPQIVELNIGFYMIGEALFVGLGETVKAMR
SAMDRGRARVIAA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=244, Percent_Identity=54.9180327868852, Blast_Score=258, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_RHOP2 (Q2IWU8)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_486223.1
- ProteinModelPortal:   Q2IWU8
- SMR:   Q2IWU8
- STRING:   Q2IWU8
- GeneID:   3910401
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_2609
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- ProtClustDB:   PRK05265
- BioCyc:   RPAL316058:RPB_2609-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 27240; Mature: 27108

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: ACT_SITE 48-48 ACT_SITE 75-75 ACT_SITE 199-199 BINDING 12-12 BINDING 23-23 BINDING 50-50 BINDING 55-55 BINDING 105-105 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSSPLRLGVNIDHIATVRNARGGRHPDPVRAALTAIEAGADGITAHLREDRRHIRDND
CCCCCCEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
MERLKAEISKPLNFEMAATPDMVRIALGVKPHAVCLVPERREELTTEGGLDVVGQRDSLA
HHHHHHHHCCCCCEEEECCCCEEEEEECCCCCEEEEECCHHHHHCCCCCCEEECCCCCCC
PSIARFNDAGIRVSLFIAADPAQIEMAAKLKAPAIELHTGAWCDAITDDEPAKVAEEWRR
HHHHHCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
IVAGAALAQSAGLEVHAGHGLDYATAETIAALPQIVELNIGFYMIGEALFVGLGETVKAM
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCHHHHHH
RSAMDRGRARVIAA
HHHHHCCCEEEEEC
>Mature Secondary Structure 
PKSSPLRLGVNIDHIATVRNARGGRHPDPVRAALTAIEAGADGITAHLREDRRHIRDND
CCCCCEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCH
MERLKAEISKPLNFEMAATPDMVRIALGVKPHAVCLVPERREELTTEGGLDVVGQRDSLA
HHHHHHHHCCCCCEEEECCCCEEEEEECCCCCEEEEECCHHHHHCCCCCCEEECCCCCCC
PSIARFNDAGIRVSLFIAADPAQIEMAAKLKAPAIELHTGAWCDAITDDEPAKVAEEWRR
HHHHHCCCCCEEEEEEEECCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHH
IVAGAALAQSAGLEVHAGHGLDYATAETIAALPQIVELNIGFYMIGEALFVGLGETVKAM
HHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHCCCHHHHHH
RSAMDRGRARVIAA
HHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA