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The map label for this gene is aspS [H]

Identifier: 86749616

GI number: 86749616

Start: 2856531

End: 2858303

Strand: Direct

Name: aspS [H]

Synonym: RPB_2496

Alternate gene names: 86749616

Gene position: 2856531-2858303 (Clockwise)

Preceding gene: 86749613

Following gene: 86749617

Centisome position: 53.58

GC content: 64.07

Gene sequence:

>1773_bases
ATGCACCGTTACCGAACCCATACTTGCGGCGCGCTCCGCGACAGCGACATCGACCAGACCGTCCGCGTGTCCGGCTGGTG
CCATCGCATCCGCGACCATGGCGGCCTTCTGTTCATCGATCTGCGCGACCATTACGGGCTCACGCAATGCGTTGCCGATC
CGGATTCGCCGGCGTTCAAGGATGCCGAGAAACTGCGCGCGGAATGGGTGGTGCGGATCGATGGCCGCGTCCGCCGTCGC
CCCGAAGGCACCGACAATGACGATCTGCCGACCGGCAAGGTCGAGATCTTCATCACCGAGATCGAAGTGCTCGGCCCGGC
CGGCGAATTGCCGCTGCCGGTGTTCGGCGAGCAGGACTATCCCGAAGATGTGCGCCTGCGGTACCGGTTTCTCGACCTGC
GACGCGAGAAGCTGCATCAGAACATCATGACCCGCGGCGCGATCGTCGACGCGATGCGCAAGCGGATGAAGGAGCAGGGC
TTCTTCGAATTCCAGACGCCGATCCTGACGGCGTCGTCGCCCGAAGGCGCGCGCGACTTCCTGGTGCCGAGCCGGATTCA
TCCGGGCAAGTTCTACGCGCTGCCGCAGGCGCCGCAGCAGTACAAGCAGCTCCTGATGATGTCCGGCTTCGATCGCTACT
TCCAGATCGCGCCGTGTTTCCGCGATGAAGATCCGCGCGCTGACCGGCTGCCGGGCGAATTCTATCAGCTCGATGTCGAG
ATGAGCTTCGTCACCCAGGACGACGTTTTCGCCGCCATGGAGCCCGTGATTACCGGCGTGTTCGAGGATTTTGCCAAAGG
CAAACCTGTGACAAAGTCGTGGCCGCGGATTCCTTATTTCGAAAGCCTACGGAAGTACGGCACCGACAAGCCGGACCTAC
GCAATCCGCTCGAGATGCAGGACGTCTCCGAGCATTTCCGCGGCTCCGGCTTCAAGGTGTTCGCGCGGATGCTCGAAGAG
GAGCGCAACCAGGTCTGGGCGATCCCCGGTCCGGGCGGCGGCAGCCGCGCGTTCTGCGATCGCATGAACTCGTGGGCGCA
GGGCGAGGGCCAACCCGGGCTCGGCTACATCATGTGGCGCGAAGGCAATGAGGGCGCGGGACCCTTGGCCAACAACATCG
GTCCCGAGCGCACCGAGGCGATCCGCGTGGCGCTCGGCCTGAAGGCTGGCGACGCCGCGTTCTTCGTCGCCGGTGATCCG
GCGAAGTTCGTGAAGTTCGCTGGCCTCGCGCGCACCAAGGTCGGCGAGGAGCTCAACCTGATCGACAAGGATCAGTTCGC
GCTCGCCTGGGTGGTCGACTTCCCGATGTACGAGTACAACGAGGACGACAAGAAGGTCGACTTCTCGCACAACCCGTTCT
CGATGCCGCAGGGTGGCATGGATGCGCTGACGTCGCAGGATCCGCTGACCATCAAGGCGTTCCAATACGACATCACCTGC
AACGGCTACGAGATTGCGTCGGGCGGCATCCGCAACCATCGCCCCGAGGCGATGGTGAAGGCCTTCGAGATCGCCGGCTA
CGGCGAGCAGGAAGTGATCGACCGCTTCGGCGGCATGTATCGCGCCTTCCAATACGGCGCGCCGCCGCATGGCGGCATGG
CGGCCGGCGTCGACCGCATCGTGATGCTGCTGTGCGGCACCAACAACCTGCGCGAGATCTCGCTCTTCCCGATGAACCAG
CGCGCGGAGGACCTGCTGATGGGCGCGCCGTCTCAGGTGGCGCCGAAGCAGTTGCGTGAGTTGCACATCCGTCTCAATCT
GCCGGAAAGCTGA

Upstream 100 bases:

>100_bases
TTTGGTTGCCGCGATGGGTCCGCTCGGGTAAACCCTGACGCTCTCCGCAAGGCTGCGGACGCTTCGATTCTGCTTTGAGA
CGGCTTTCCCCGGGATTTCC

Downstream 100 bases:

>100_bases
ACACGGCCGAGTCGACGATCCACATGCCGACGAAGCTGCCGGGAGAATTCATGGCGTCCAATTCCGAAGACCTGCAAGCT
GCGGCGCTGGCCTATCACCG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MHRYRTHTCGALRDSDIDQTVRVSGWCHRIRDHGGLLFIDLRDHYGLTQCVADPDSPAFKDAEKLRAEWVVRIDGRVRRR
PEGTDNDDLPTGKVEIFITEIEVLGPAGELPLPVFGEQDYPEDVRLRYRFLDLRREKLHQNIMTRGAIVDAMRKRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVTQDDVFAAMEPVITGVFEDFAKGKPVTKSWPRIPYFESLRKYGTDKPDLRNPLEMQDVSEHFRGSGFKVFARMLEE
ERNQVWAIPGPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGNEGAGPLANNIGPERTEAIRVALGLKAGDAAFFVAGDP
AKFVKFAGLARTKVGEELNLIDKDQFALAWVVDFPMYEYNEDDKKVDFSHNPFSMPQGGMDALTSQDPLTIKAFQYDITC
NGYEIASGGIRNHRPEAMVKAFEIAGYGEQEVIDRFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTNNLREISLFPMNQ
RAEDLLMGAPSQVAPKQLRELHIRLNLPES

Sequences:

>Translated_590_residues
MHRYRTHTCGALRDSDIDQTVRVSGWCHRIRDHGGLLFIDLRDHYGLTQCVADPDSPAFKDAEKLRAEWVVRIDGRVRRR
PEGTDNDDLPTGKVEIFITEIEVLGPAGELPLPVFGEQDYPEDVRLRYRFLDLRREKLHQNIMTRGAIVDAMRKRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVTQDDVFAAMEPVITGVFEDFAKGKPVTKSWPRIPYFESLRKYGTDKPDLRNPLEMQDVSEHFRGSGFKVFARMLEE
ERNQVWAIPGPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGNEGAGPLANNIGPERTEAIRVALGLKAGDAAFFVAGDP
AKFVKFAGLARTKVGEELNLIDKDQFALAWVVDFPMYEYNEDDKKVDFSHNPFSMPQGGMDALTSQDPLTIKAFQYDITC
NGYEIASGGIRNHRPEAMVKAFEIAGYGEQEVIDRFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTNNLREISLFPMNQ
RAEDLLMGAPSQVAPKQLRELHIRLNLPES
>Mature_590_residues
MHRYRTHTCGALRDSDIDQTVRVSGWCHRIRDHGGLLFIDLRDHYGLTQCVADPDSPAFKDAEKLRAEWVVRIDGRVRRR
PEGTDNDDLPTGKVEIFITEIEVLGPAGELPLPVFGEQDYPEDVRLRYRFLDLRREKLHQNIMTRGAIVDAMRKRMKEQG
FFEFQTPILTASSPEGARDFLVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
MSFVTQDDVFAAMEPVITGVFEDFAKGKPVTKSWPRIPYFESLRKYGTDKPDLRNPLEMQDVSEHFRGSGFKVFARMLEE
ERNQVWAIPGPGGGSRAFCDRMNSWAQGEGQPGLGYIMWREGNEGAGPLANNIGPERTEAIRVALGLKAGDAAFFVAGDP
AKFVKFAGLARTKVGEELNLIDKDQFALAWVVDFPMYEYNEDDKKVDFSHNPFSMPQGGMDALTSQDPLTIKAFQYDITC
NGYEIASGGIRNHRPEAMVKAFEIAGYGEQEVIDRFGGMYRAFQYGAPPHGGMAAGVDRIVMLLCGTNNLREISLFPMNQ
RAEDLLMGAPSQVAPKQLRELHIRLNLPES

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=34.9498327759197, Blast_Score=341, Evalue=9e-94,
Organism=Homo sapiens, GI45439306, Length=321, Percent_Identity=27.1028037383178, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI4758762, Length=251, Percent_Identity=27.4900398406374, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI226491502, Length=247, Percent_Identity=25.5060728744939, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI194272210, Length=266, Percent_Identity=28.9473684210526, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI5031815, Length=266, Percent_Identity=28.9473684210526, Blast_Score=77, Evalue=6e-14,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=41.3265306122449, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1790571, Length=220, Percent_Identity=30.4545454545455, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789256, Length=220, Percent_Identity=29.5454545454545, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=604, Percent_Identity=32.6158940397351, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71984122, Length=299, Percent_Identity=27.4247491638796, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=275, Percent_Identity=26.9090909090909, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=649, Percent_Identity=27.7349768875193, Blast_Score=211, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321807, Length=267, Percent_Identity=28.4644194756554, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323011, Length=273, Percent_Identity=26.7399267399267, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=294, Percent_Identity=26.8707482993197, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=30.9602649006623, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=26.8939393939394, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921528, Length=232, Percent_Identity=28.8793103448276, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24640849, Length=262, Percent_Identity=25.5725190839695, Blast_Score=70, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24640851, Length=261, Percent_Identity=25.2873563218391, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66701; Mature: 66701

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRTHTCGALRDSDIDQTVRVSGWCHRIRDHGGLLFIDLRDHYGLTQCVADPDSPAFK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCHH
DAEKLRAEWVVRIDGRVRRRPEGTDNDDLPTGKVEIFITEIEVLGPAGELPLPVFGEQDY
HHHHHHHEEEEEECCEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCC
PEDVRLRYRFLDLRREKLHQNIMTRGAIVDAMRKRMKEQGFFEFQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQDDVFAAMEPVITGVFEDFAKGKPVTKSWPRIPYFESLRKYGTDKPDLRNPLEMQ
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVSEHFRGSGFKVFARMLEEERNQVWAIPGPGGGSRAFCDRMNSWAQGEGQPGLGYIMWR
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCHHCCCCCCCCCEEEEEE
EGNEGAGPLANNIGPERTEAIRVALGLKAGDAAFFVAGDPAKFVKFAGLARTKVGEELNL
CCCCCCCCCHHCCCCCHHHEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCCC
IDKDQFALAWVVDFPMYEYNEDDKKVDFSHNPFSMPQGGMDALTSQDPLTIKAFQYDITC
CCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCEEEEEEEEEEEE
NGYEIASGGIRNHRPEAMVKAFEIAGYGEQEVIDRFGGMYRAFQYGAPPHGGMAAGVDRI
CCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHH
VMLLCGTNNLREISLFPMNQRAEDLLMGAPSQVAPKQLRELHIRLNLPES
EEEEECCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHEEEEEEECCCC
>Mature Secondary Structure
MHRYRTHTCGALRDSDIDQTVRVSGWCHRIRDHGGLLFIDLRDHYGLTQCVADPDSPAFK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCHH
DAEKLRAEWVVRIDGRVRRRPEGTDNDDLPTGKVEIFITEIEVLGPAGELPLPVFGEQDY
HHHHHHHEEEEEECCEEECCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCC
PEDVRLRYRFLDLRREKLHQNIMTRGAIVDAMRKRMKEQGFFEFQTPILTASSPEGARDF
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEECCCCCCCHHC
LVPSRIHPGKFYALPQAPQQYKQLLMMSGFDRYFQIAPCFRDEDPRADRLPGEFYQLDVE
CCCCCCCCCCEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVTQDDVFAAMEPVITGVFEDFAKGKPVTKSWPRIPYFESLRKYGTDKPDLRNPLEMQ
EEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
DVSEHFRGSGFKVFARMLEEERNQVWAIPGPGGGSRAFCDRMNSWAQGEGQPGLGYIMWR
HHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHCHHCCCCCCCCCEEEEEE
EGNEGAGPLANNIGPERTEAIRVALGLKAGDAAFFVAGDPAKFVKFAGLARTKVGEELNL
CCCCCCCCCHHCCCCCHHHEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHCCCCCCC
IDKDQFALAWVVDFPMYEYNEDDKKVDFSHNPFSMPQGGMDALTSQDPLTIKAFQYDITC
CCCCCEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCEEEEEEEEEEEE
NGYEIASGGIRNHRPEAMVKAFEIAGYGEQEVIDRFGGMYRAFQYGAPPHGGMAAGVDRI
CCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHH
VMLLCGTNNLREISLFPMNQRAEDLLMGAPSQVAPKQLRELHIRLNLPES
EEEEECCCCCEEEEEECCCCCHHHHHCCCCCCCCHHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA