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The map label for this gene is dme [H]

Identifier: 86749617

GI number: 86749617

Start: 2858354

End: 2860663

Strand: Direct

Name: dme [H]

Synonym: RPB_2497

Alternate gene names: 86749617

Gene position: 2858354-2860663 (Clockwise)

Preceding gene: 86749616

Following gene: 86749618

Centisome position: 53.61

GC content: 67.14

Gene sequence:

>2310_bases
ATGGCGTCCAATTCCGAAGACCTGCAAGCTGCGGCGCTGGCCTATCACCGGCTGCCGCGGCCCGGCAAACTCGAAGTGCA
TGCGTCGAAGCCGCTGGCCAACCAGCGCGACCTCGCGCTCGCCTACACACCGGGCGTCGCCGCCGCCTGCAACGCGATCG
CGGCCGATCCGGCCCAGGCCGCGTCGCTCACGGTGCGCGGCAATCTGGTCGCCGTGGTCACCAACGGCACCGCGGTGCTC
GGTCTCGGCAATATCGGTCCGCTCGCCTCCAAGCCGGTGATGGAGGGCAAGGCGGTCCTGTTCAAGAAGTTCGCCGACAT
CAACGTGTTCGACATCGAGATCGCGGCCGACACGGTCGAGCGCGTGGTCGAGACGGTGGCGGCGCTGGAGCCGACCTTCG
GCGGCATTAATCTCGAGGACATCAAGGGACCGGAGTGCTTCGAGATCGAGGCGCAGCTCAAGGAGCGCATGAAGATCCCG
GTGTTTCACGACGATCAGCACGGCACTGCGATCATCGTCGGCGCGGCGGTCAAGAATGCGCTGCTGCTGAAGGGCAAGGA
GCTCAGCGAGGTCAAGATCGTCACCGCCGGCGCCGGCGCCGCGGCGCTGGCCTGTCTCAATCTTCTCGTGTCGCTCGGCG
CGCAGCGCAAGAACATCTGGGTCTGCGATCTCGAAGGCCTGGTGTACGAAGGCCGCACCGTGCTGATGGATCGCTGGAAG
GCGGTCTACGCGCAGAAGACCGACAAGCGGAAGCTCGCCGACGTGATCGGCGGCGCCGACGTGTTTCTCGGCCTGTCTGG
CCCGAAAGTGCTGACACCCGAGATGGTCATGCAGATGGCCGACAAGCCGCTGGTGATGGCGCTCGCCAATCCGACGCCGG
AGATCATGCCGGACGAAGTCCGCCGCGTGCGGCCCGACGCGTTGATCTGCACCGGACGCTCCGACTTCCCGAACCAGGTC
AACAACGTCCTGTGCTTTCCCTACATCTTCCGTGGCGCGCTCGATGTCGGCGCCACCGCGATCAACGAGGAGATGAAGAT
CGCCGCGGTCGATGCGATCGCCCAGCTCGCGCGCGATCCGCCGGCCGATCCGGTGGCGGGCAGCCTCGACACCGACGAAG
TGCTGGGCTTCGGCGAAGGTTCGCTGATCCCCAGTCCATTCGATCCGCGGCTGATCCTGCGCATCGCGCCCGCGGTGGCG
ATGGCGGCGATGGATTCCGGCGTCGCGACCCGGCCGATCACCTCGTTCGACGATTACTACGCCCAGCTCGAGCGCTTCGC
GTTCCGCTCCGGCCTGGTGATGAAGCCGCTGTTCGCCAAGGCCAAGGCGCAGCCGGTGCGGGTGATCTATGCCGAAGGCG
AGGACGAGCGCGTGCTGCACGCCGTGCAGGTGATCCTCGAGGAAAAGCTGGCGCGGCCCATTCTGGTCGGGCGGCCCTCG
GTGGTCGAGGCGCGGATCAAGCGGTCCGGCCTGTCGATCAAGCCCGGCGTGGATTTCGACCTGGTCAATCCGCAGGACGA
TCCGCGTTACCGCTCCTACGTGCAGTCCTATATCGACATTGCCGGTCGCGCCGGCGTGACGCCGGCGGCGGCCCGCACGG
TGGTGCGCACCAATTCGACCGTGATCGCCGCGCTGGCGGTGGCGCGCGGCGAGGCGGACGCGATGATCTGCGGCCTCGAC
GGCCGTTACATGAATCATCTGCGTCATGTCCGCGACGTGATTGGTTCCTTGCCGGGCGTCTCGGACTACGCGGCGCTGGC
GCTGCTGATCACCAGCAAGGGCGCGATCTTCATCGCCGATACCCAGGTGCGGCCCAATCCGACCGCCGAGGAACTGGCCG
AGGTGGCCGCGCTCGCCGCGGTCCACGTCCAGCGCTTCAATATCAAGCCGCGGATCGCGTTCCTGTCGCATTCGGATTTC
GGCAGCTATCCGACCGAATCGTCGATGAAGATGCGGCGCGCCACGGCGATCCTCGCCGAGCGCCATCCGGAGATCGAGGC
CGACGGCGAGATGCAGGGCGACACCGCGCTGTCGCAGACCGCGCGCGAACTGATCCTGCCGCATTCCCGGCTCGAGGGCG
GCGCCAACGTGCTGATCATGCCGAATCTCGATACCGCCAACGTCGCCTACCAGATGATCAAGGTGCTGGGCGACGCCTTG
CCGGTGGGCCCGATCCTGATCGGACCGGCGCGGCCGGCGCACATCCTGACGCCCTCGGTGACGGCGCGCGGCATCCTCAA
TATGACAGCGGTGGCCGTCGTCGAGGCGCAGGAGCGGGCAGGGCGGAGTCAGCGCACGCTGTTCGGCTAA

Upstream 100 bases:

>100_bases
CGAAGCAGTTGCGTGAGTTGCACATCCGTCTCAATCTGCCGGAAAGCTGAACACGGCCGAGTCGACGATCCACATGCCGA
CGAAGCTGCCGGGAGAATTC

Downstream 100 bases:

>100_bases
GTGGATCGGCCGGCGGGCCGGCGCGAACGGACCCCTCGATCCTGAATCGGCTTCGCCGTGGTGCGTAACATTTCGCGCGA
TCATTTGATCGTTTGAAAAC

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 769; Mature: 768

Protein sequence:

>769_residues
MASNSEDLQAAALAYHRLPRPGKLEVHASKPLANQRDLALAYTPGVAAACNAIAADPAQAASLTVRGNLVAVVTNGTAVL
GLGNIGPLASKPVMEGKAVLFKKFADINVFDIEIAADTVERVVETVAALEPTFGGINLEDIKGPECFEIEAQLKERMKIP
VFHDDQHGTAIIVGAAVKNALLLKGKELSEVKIVTAGAGAAALACLNLLVSLGAQRKNIWVCDLEGLVYEGRTVLMDRWK
AVYAQKTDKRKLADVIGGADVFLGLSGPKVLTPEMVMQMADKPLVMALANPTPEIMPDEVRRVRPDALICTGRSDFPNQV
NNVLCFPYIFRGALDVGATAINEEMKIAAVDAIAQLARDPPADPVAGSLDTDEVLGFGEGSLIPSPFDPRLILRIAPAVA
MAAMDSGVATRPITSFDDYYAQLERFAFRSGLVMKPLFAKAKAQPVRVIYAEGEDERVLHAVQVILEEKLARPILVGRPS
VVEARIKRSGLSIKPGVDFDLVNPQDDPRYRSYVQSYIDIAGRAGVTPAAARTVVRTNSTVIAALAVARGEADAMICGLD
GRYMNHLRHVRDVIGSLPGVSDYAALALLITSKGAIFIADTQVRPNPTAEELAEVAALAAVHVQRFNIKPRIAFLSHSDF
GSYPTESSMKMRRATAILAERHPEIEADGEMQGDTALSQTARELILPHSRLEGGANVLIMPNLDTANVAYQMIKVLGDAL
PVGPILIGPARPAHILTPSVTARGILNMTAVAVVEAQERAGRSQRTLFG

Sequences:

>Translated_769_residues
MASNSEDLQAAALAYHRLPRPGKLEVHASKPLANQRDLALAYTPGVAAACNAIAADPAQAASLTVRGNLVAVVTNGTAVL
GLGNIGPLASKPVMEGKAVLFKKFADINVFDIEIAADTVERVVETVAALEPTFGGINLEDIKGPECFEIEAQLKERMKIP
VFHDDQHGTAIIVGAAVKNALLLKGKELSEVKIVTAGAGAAALACLNLLVSLGAQRKNIWVCDLEGLVYEGRTVLMDRWK
AVYAQKTDKRKLADVIGGADVFLGLSGPKVLTPEMVMQMADKPLVMALANPTPEIMPDEVRRVRPDALICTGRSDFPNQV
NNVLCFPYIFRGALDVGATAINEEMKIAAVDAIAQLARDPPADPVAGSLDTDEVLGFGEGSLIPSPFDPRLILRIAPAVA
MAAMDSGVATRPITSFDDYYAQLERFAFRSGLVMKPLFAKAKAQPVRVIYAEGEDERVLHAVQVILEEKLARPILVGRPS
VVEARIKRSGLSIKPGVDFDLVNPQDDPRYRSYVQSYIDIAGRAGVTPAAARTVVRTNSTVIAALAVARGEADAMICGLD
GRYMNHLRHVRDVIGSLPGVSDYAALALLITSKGAIFIADTQVRPNPTAEELAEVAALAAVHVQRFNIKPRIAFLSHSDF
GSYPTESSMKMRRATAILAERHPEIEADGEMQGDTALSQTARELILPHSRLEGGANVLIMPNLDTANVAYQMIKVLGDAL
PVGPILIGPARPAHILTPSVTARGILNMTAVAVVEAQERAGRSQRTLFG
>Mature_768_residues
ASNSEDLQAAALAYHRLPRPGKLEVHASKPLANQRDLALAYTPGVAAACNAIAADPAQAASLTVRGNLVAVVTNGTAVLG
LGNIGPLASKPVMEGKAVLFKKFADINVFDIEIAADTVERVVETVAALEPTFGGINLEDIKGPECFEIEAQLKERMKIPV
FHDDQHGTAIIVGAAVKNALLLKGKELSEVKIVTAGAGAAALACLNLLVSLGAQRKNIWVCDLEGLVYEGRTVLMDRWKA
VYAQKTDKRKLADVIGGADVFLGLSGPKVLTPEMVMQMADKPLVMALANPTPEIMPDEVRRVRPDALICTGRSDFPNQVN
NVLCFPYIFRGALDVGATAINEEMKIAAVDAIAQLARDPPADPVAGSLDTDEVLGFGEGSLIPSPFDPRLILRIAPAVAM
AAMDSGVATRPITSFDDYYAQLERFAFRSGLVMKPLFAKAKAQPVRVIYAEGEDERVLHAVQVILEEKLARPILVGRPSV
VEARIKRSGLSIKPGVDFDLVNPQDDPRYRSYVQSYIDIAGRAGVTPAAARTVVRTNSTVIAALAVARGEADAMICGLDG
RYMNHLRHVRDVIGSLPGVSDYAALALLITSKGAIFIADTQVRPNPTAEELAEVAALAAVHVQRFNIKPRIAFLSHSDFG
SYPTESSMKMRRATAILAERHPEIEADGEMQGDTALSQTARELILPHSRLEGGANVLIMPNLDTANVAYQMIKVLGDALP
VGPILIGPARPAHILTPSVTARGILNMTAVAVVEAQERAGRSQRTLFG

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=399, Percent_Identity=25.8145363408521, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI62420880, Length=399, Percent_Identity=25.8145363408521, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI239049447, Length=399, Percent_Identity=25.8145363408521, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4505143, Length=400, Percent_Identity=22.75, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI4505145, Length=401, Percent_Identity=25.1870324189526, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI270265879, Length=369, Percent_Identity=25.7452574525745, Blast_Score=70, Evalue=1e-11,
Organism=Escherichia coli, GI1788806, Length=756, Percent_Identity=57.4074074074074, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=324, Percent_Identity=32.4074074074074, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1788635, Length=347, Percent_Identity=26.2247838616715, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI87081919, Length=318, Percent_Identity=28.3018867924528, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362674, Length=501, Percent_Identity=24.750499001996, Blast_Score=83, Evalue=9e-16,
Organism=Drosophila melanogaster, GI21356279, Length=501, Percent_Identity=24.750499001996, Blast_Score=83, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281362672, Length=501, Percent_Identity=24.750499001996, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 82142; Mature: 82011

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNSEDLQAAALAYHRLPRPGKLEVHASKPLANQRDLALAYTPGVAAACNAIAADPAQA
CCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCHH
ASLTVRGNLVAVVTNGTAVLGLGNIGPLASKPVMEGKAVLFKKFADINVFDIEIAADTVE
CEEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHHH
RVVETVAALEPTFGGINLEDIKGPECFEIEAQLKERMKIPVFHDDQHGTAIIVGAAVKNA
HHHHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHCCCCCEEECCCCCCEEEEEHHHHCC
LLLKGKELSEVKIVTAGAGAAALACLNLLVSLGAQRKNIWVCDLEGLVYEGRTVLMDRWK
CEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCHHHHHHHH
AVYAQKTDKRKLADVIGGADVFLGLSGPKVLTPEMVMQMADKPLVMALANPTPEIMPDEV
HHHHCCCHHHHHHHHHCCCEEEEECCCCCEECHHHHHHHCCCCEEEEECCCCCCCCCHHH
RRVRPDALICTGRSDFPNQVNNVLCFPYIFRGALDVGATAINEEMKIAAVDAIAQLARDP
HHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHCCC
PADPVAGSLDTDEVLGFGEGSLIPSPFDPRLILRIAPAVAMAAMDSGVATRPITSFDDYY
CCCCCCCCCCHHHHEECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHH
AQLERFAFRSGLVMKPLFAKAKAQPVRVIYAEGEDERVLHAVQVILEEKLARPILVGRPS
HHHHHHHHHCCCEECHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCH
VVEARIKRSGLSIKPGVDFDLVNPQDDPRYRSYVQSYIDIAGRAGVTPAAARTVVRTNST
HHHHHHHHCCCEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEEECCCC
VIAALAVARGEADAMICGLDGRYMNHLRHVRDVIGSLPGVSDYAALALLITSKGAIFIAD
EEEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECCCEEEEEE
TQVRPNPTAEELAEVAALAAVHVQRFNIKPRIAFLSHSDFGSYPTESSMKMRRATAILAE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
RHPEIEADGEMQGDTALSQTARELILPHSRLEGGANVLIMPNLDTANVAYQMIKVLGDAL
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCC
PVGPILIGPARPAHILTPSVTARGILNMTAVAVVEAQERAGRSQRTLFG
CCCCEEECCCCCCEEECCCHHHHHHHHHHHHHEEECHHHCCCCCCCCCC
>Mature Secondary Structure 
ASNSEDLQAAALAYHRLPRPGKLEVHASKPLANQRDLALAYTPGVAAACNAIAADPAQA
CCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCCHH
ASLTVRGNLVAVVTNGTAVLGLGNIGPLASKPVMEGKAVLFKKFADINVFDIEIAADTVE
CEEEEECCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECHHHHH
RVVETVAALEPTFGGINLEDIKGPECFEIEAQLKERMKIPVFHDDQHGTAIIVGAAVKNA
HHHHHHHHCCCCCCCCCHHHCCCCCEEEEHHHHHHCCCCCEEECCCCCCEEEEEHHHHCC
LLLKGKELSEVKIVTAGAGAAALACLNLLVSLGAQRKNIWVCDLEGLVYEGRTVLMDRWK
CEECCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEECCCHHHHHHHH
AVYAQKTDKRKLADVIGGADVFLGLSGPKVLTPEMVMQMADKPLVMALANPTPEIMPDEV
HHHHCCCHHHHHHHHHCCCEEEEECCCCCEECHHHHHHHCCCCEEEEECCCCCCCCCHHH
RRVRPDALICTGRSDFPNQVNNVLCFPYIFRGALDVGATAINEEMKIAAVDAIAQLARDP
HHCCCCEEEEECCCCCCHHHHCEEEHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHCCC
PADPVAGSLDTDEVLGFGEGSLIPSPFDPRLILRIAPAVAMAAMDSGVATRPITSFDDYY
CCCCCCCCCCHHHHEECCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCHHHHH
AQLERFAFRSGLVMKPLFAKAKAQPVRVIYAEGEDERVLHAVQVILEEKLARPILVGRPS
HHHHHHHHHCCCEECHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCEEECCCH
VVEARIKRSGLSIKPGVDFDLVNPQDDPRYRSYVQSYIDIAGRAGVTPAAARTVVRTNST
HHHHHHHHCCCEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHEEEECCCC
VIAALAVARGEADAMICGLDGRYMNHLRHVRDVIGSLPGVSDYAALALLITSKGAIFIAD
EEEEEHHHCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHEEEEEECCCEEEEEE
TQVRPNPTAEELAEVAALAAVHVQRFNIKPRIAFLSHSDFGSYPTESSMKMRRATAILAE
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
RHPEIEADGEMQGDTALSQTARELILPHSRLEGGANVLIMPNLDTANVAYQMIKVLGDAL
CCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHCC
PVGPILIGPARPAHILTPSVTARGILNMTAVAVVEAQERAGRSQRTLFG
CCCCEEECCCCCCEEECCCHHHHHHHHHHHHHEEECHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]