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The map label for this gene is pyrB [H]

Identifier: 86749544

GI number: 86749544

Start: 2780383

End: 2781336

Strand: Reverse

Name: pyrB [H]

Synonym: RPB_2424

Alternate gene names: 86749544

Gene position: 2781336-2780383 (Counterclockwise)

Preceding gene: 86749548

Following gene: 86749543

Centisome position: 52.17

GC content: 66.25

Gene sequence:

>954_bases
ATGACCCCCGCATTGAAATCGACCTTTGTCCTCGCTCACCGGCATCTGCTGGGGATCGAAGGGCTTTCCGCCGCCGACAT
CACCGGCCTGCTCGACCTTTCCGAGGAATATGTCGAGCTCAACCGGCAGGTCGACAAGAAGCGCGCCTCGTTGCGCGGCC
GCACCCAGGTCAATCTGTTCTTCGAGGCTTCGACGCGAACGCAATCGTCGTTCGAGATCGCCGGCAAAAGGCTCGGCGCC
GACGTCATGAACATGTCGGTGTCGTCGTCCTCGATGCGCAAGGGCGAGACCCTGATGGACACCGCGGTGACGCTGAACGC
GATGCACCCGGACATCCTCGTGGTCCGCCATCACGCCTCCGGCGCGGTCGAACTGCTGGCGCGAAAAGTCGACGGCTCGG
TGATCAATGCCGGCGACGGCGCGCACGAGCATCCGACCCAGGCGCTGCTCGATGCGCTGACGATCCGCCGCAACAAGGGC
CGGCTCGAAGGCCTGGTGGTCGCGATCTGCGGCGACGTGATGCATTCGCGCGTCGCCCGCTCCAACATCCTGCTGCTCAA
CACCATGGGCGCCCGCGTCCGCGTGGTCGCGCCCTCGACGCTGCTGCCCCGCGGCATCGAGCGCATGGGCGTCGAGGTCG
CGCGCGACATGCGCGAAGGGCTCGACGGCGCGGATATCGTCATGATGCTGCGGCTGCAGCGCGAGCGGATGAACGGCTCG
TTCGTGCCGTCGAGCGGCGAATATTTCCACTATTTCGGGCTCGACCAGAAGAAGCTGGCCTACGCCAAGCCCGACGCGCT
GGTGATGCATCCGGGCCCGATGAATCGCGGCGTCGAGATCGACTCGATCGTCGCCGACGGCGCGCAGTCGGTGATCCGCG
AACAGGTCGAAATGGGCGTGGCGGTGCGGATGGCGGTGCTCGAAGCGCTCGCCCGCAACCTGCCCAACGCCTGA

Upstream 100 bases:

>100_bases
ATACACCGGGCCTCTGTTCCACAGCACCGGGGGATATTCTGCGCGGGCCTGCGGATCGCGCTTGCCCGCTCCGGCAAGTC
TCCCTATAGCTCTTGCTTTA

Downstream 100 bases:

>100_bases
AGCTGGAACTGGACCGACAATGCTGATCGACCGCCGCCCGATCCTGCTCGCCAACGCCCGCCTGATCGATCCGGCGCGCG
ACTTCGACGGCCTCGGCGAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTPALKSTFVLAHRHLLGIEGLSAADITGLLDLSEEYVELNRQVDKKRASLRGRTQVNLFFEASTRTQSSFEIAGKRLGA
DVMNMSVSSSSMRKGETLMDTAVTLNAMHPDILVVRHHASGAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKG
RLEGLVVAICGDVMHSRVARSNILLLNTMGARVRVVAPSTLLPRGIERMGVEVARDMREGLDGADIVMMLRLQRERMNGS
FVPSSGEYFHYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSVIREQVEMGVAVRMAVLEALARNLPNA

Sequences:

>Translated_317_residues
MTPALKSTFVLAHRHLLGIEGLSAADITGLLDLSEEYVELNRQVDKKRASLRGRTQVNLFFEASTRTQSSFEIAGKRLGA
DVMNMSVSSSSMRKGETLMDTAVTLNAMHPDILVVRHHASGAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKG
RLEGLVVAICGDVMHSRVARSNILLLNTMGARVRVVAPSTLLPRGIERMGVEVARDMREGLDGADIVMMLRLQRERMNGS
FVPSSGEYFHYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSVIREQVEMGVAVRMAVLEALARNLPNA
>Mature_316_residues
TPALKSTFVLAHRHLLGIEGLSAADITGLLDLSEEYVELNRQVDKKRASLRGRTQVNLFFEASTRTQSSFEIAGKRLGAD
VMNMSVSSSSMRKGETLMDTAVTLNAMHPDILVVRHHASGAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKGR
LEGLVVAICGDVMHSRVARSNILLLNTMGARVRVVAPSTLLPRGIERMGVEVARDMREGLDGADIVMMLRLQRERMNGSF
VPSSGEYFHYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSVIREQVEMGVAVRMAVLEALARNLPNA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=323, Percent_Identity=36.5325077399381, Blast_Score=166, Evalue=3e-41,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=33.6538461538462, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1790703, Length=235, Percent_Identity=28.936170212766, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1786469, Length=237, Percent_Identity=28.6919831223629, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=33.6391437308869, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=35.1791530944625, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24642586, Length=308, Percent_Identity=33.1168831168831, Blast_Score=147, Evalue=1e-35,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 34514; Mature: 34382

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPALKSTFVLAHRHLLGIEGLSAADITGLLDLSEEYVELNRQVDKKRASLRGRTQVNLF
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
FEASTRTQSSFEIAGKRLGADVMNMSVSSSSMRKGETLMDTAVTLNAMHPDILVVRHHAS
EEECCCCCCHHHHHHHHHCHHHHHCCCCCHHHHCCCHHHHHHHEEECCCCCEEEEEECCC
GAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKGRLEGLVVAICGDVMHSRVAR
CHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SNILLLNTMGARVRVVAPSTLLPRGIERMGVEVARDMREGLDGADIVMMLRLQRERMNGS
CCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
FVPSSGEYFHYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSVIREQVEMGV
CCCCCCCEEEEECCCCHHHEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRMAVLEALARNLPNA
HHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPALKSTFVLAHRHLLGIEGLSAADITGLLDLSEEYVELNRQVDKKRASLRGRTQVNLF
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
FEASTRTQSSFEIAGKRLGADVMNMSVSSSSMRKGETLMDTAVTLNAMHPDILVVRHHAS
EEECCCCCCHHHHHHHHHCHHHHHCCCCCHHHHCCCHHHHHHHEEECCCCCEEEEEECCC
GAVELLARKVDGSVINAGDGAHEHPTQALLDALTIRRNKGRLEGLVVAICGDVMHSRVAR
CHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SNILLLNTMGARVRVVAPSTLLPRGIERMGVEVARDMREGLDGADIVMMLRLQRERMNGS
CCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
FVPSSGEYFHYFGLDQKKLAYAKPDALVMHPGPMNRGVEIDSIVADGAQSVIREQVEMGV
CCCCCCCEEEEECCCCHHHEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AVRMAVLEALARNLPNA
HHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA