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The map label for this gene is ilvD [H]

Identifier: 86749216

GI number: 86749216

Start: 2380537

End: 2382345

Strand: Reverse

Name: ilvD [H]

Synonym: RPB_2095

Alternate gene names: 86749216

Gene position: 2382345-2380537 (Counterclockwise)

Preceding gene: 86749224

Following gene: 86749215

Centisome position: 44.68

GC content: 66.72

Gene sequence:

>1809_bases
ATGACCAAGATCACCCCGGGGACCGCCCGACGCAAACTGCGTTCCAGCGAATGGTTCAACGATCCGCACAATCCGGCGAT
GACCGCGCTGTATCTCGAGCGCTATCTGAACTACGGACTGACGCGCGGCGAACTGCAGTCCGGCAAGCCGATCATCGGCA
TCGCCCAGACCGGCAACGACCTGTCGCCGTGCAACCGCCATCATCTGGAACTGGCGCAGCGCGTGCGCGAAGGCATCCGC
GCCGCCGGCGGCATCGCGATGGAATTCCCGGTGCACCCGATCCAGGAAACCGGCAAGCGGCCGACCGCGGCGCTGGATCG
CAATCTGGCTTATCTCGGTCTCGTCGAGGTGCTGTTCAGCTATCCGCTCGACGGCGTGGTGCTCACCACCGGCTGCGACA
AGACCACGCCGGCCTGCCTGATGGCGGCGGCGACCGTGAACATCCCGGCGATCGTGCTGTCCGGCGGGCCGATGCTGAAC
GGCTGGCACAATGGCGAGCGCTCCGGCTCCGGCACGGTGGTGTGGAAATCACGCGAGCGTCTCGCCGCCGGCGAGATCGA
CTACGAGGAATTCATGGAGATCGTGGCGTCGTCGGCGCCGTCGGTCGGCCATTGCAACACCATGGGCACCGCGTCGACGA
TGAACTCGCTGGCCGAAGCGCTCGGCATGTCGCTGCCCGGCTGCGCCGCGATCCCGGCGCCGTATCGCGAGCGCGGCCAG
ATCGCCTACGCCACCGGCGTGCGCGCGGTCGAGATGGTGTGGGAGGATCTGAAGCCGTCCGACATCCTGACGCGCGAGGC
GTTCGAAAACGCCATCGTGGTCAATTCGGCGATCGGCGGCTCGACCAACGCGCCGATCCATCTCAACGCGCTGGCGCGCC
ACATCGGCGTCGAGCTTTCGATCGACGACTGGCAGAGCATCGGCCACAAAATTCCGCTGCTGGTCAACATGCAGCCGGCG
GGATTCTATCTCGGCGAGGAATATCACCGCGCCGGCGGCGTGCCGGCGGTGGTGCGCGAGTTGATGCAGCACGGCAAGAT
TCACACGGACGCGCTGACGGTGAACGGCCTCACCATGGGCCAGAACTGTGCCGGCGCGCCCGCGCCCGACGGCGACGTGA
TCAAGTCCTACGACGGACCGCTGGTGCAGGACGCAGGCTTCCTGGTGCTGCGCGGCAATCTGTTCGAGTCCGCGATCATG
AAGACATCCGTGATCAGCCTCGAATTCCGCGAGCGCTATCTGTCCAGCCCGAACGACCCCAACGCGTTCGAGGGCCGCGC
CATCGTGTTCGAGGGGCCGGAGGATTATCACGACCGGATCGACGATCCGGCGCTGAAGATCGACGAGCACTGCATCCTGT
TCGTGCGCGGCACCGGGCCGATCGGCTATCCGGGTGGCGCCGAAGTCGTGAACATGCAGCCGCCGGCGGCGCTGATCAAG
CGCGGCATTCACTCCCTGCCCTGCATCGGCGACGGACGCCAGAGCGGCACCTCGGGCTCGCCGTCGATCCTCAACGCGAC
GCCGGAGGCCGCCGCCAATGGCGGGCTCGCGATCCTGAAGACCGGCGACCGCGTCCGCATCGATCTGAACACGGGCAGCG
CCAATATTCTGATCAGCGACGACGAGTTGAAGCAGCGCCGCGCCGAACTCGAGGCGCATGGCGGCTTCGCCTACCCGAAG
CACCAGACGCCGTGGCAGGAACTGTATCGCCAGACCGTGGGCCAACAGGCCACCGGCGCCTGCCTCGAACTCGCGACGCG
CTACCACGACATCGCCGGCACGGTCGGCGTGGCGCGGCATAATCATTGA

Upstream 100 bases:

>100_bases
GAGCCGCACACGGCATGTCAGCATGCGCCGCCGCCGAATGAATCACCATCAATAAGCTCGAGACGCCCGAACAACGGGCG
TGTGCGCTGCGGGAGAAACG

Downstream 100 bases:

>100_bases
TGCGCCCGTGCTGACGAAGGACCTCTCCTCGCGTAGCAGCCGAACCTCTCCCCGCGTGCGGGGAGAGGTCGGAAGTTGCG
CGCAGCGCGACTTCCGGGTG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 602; Mature: 601

Protein sequence:

>602_residues
MTKITPGTARRKLRSSEWFNDPHNPAMTALYLERYLNYGLTRGELQSGKPIIGIAQTGNDLSPCNRHHLELAQRVREGIR
AAGGIAMEFPVHPIQETGKRPTAALDRNLAYLGLVEVLFSYPLDGVVLTTGCDKTTPACLMAAATVNIPAIVLSGGPMLN
GWHNGERSGSGTVVWKSRERLAAGEIDYEEFMEIVASSAPSVGHCNTMGTASTMNSLAEALGMSLPGCAAIPAPYRERGQ
IAYATGVRAVEMVWEDLKPSDILTREAFENAIVVNSAIGGSTNAPIHLNALARHIGVELSIDDWQSIGHKIPLLVNMQPA
GFYLGEEYHRAGGVPAVVRELMQHGKIHTDALTVNGLTMGQNCAGAPAPDGDVIKSYDGPLVQDAGFLVLRGNLFESAIM
KTSVISLEFRERYLSSPNDPNAFEGRAIVFEGPEDYHDRIDDPALKIDEHCILFVRGTGPIGYPGGAEVVNMQPPAALIK
RGIHSLPCIGDGRQSGTSGSPSILNATPEAAANGGLAILKTGDRVRIDLNTGSANILISDDELKQRRAELEAHGGFAYPK
HQTPWQELYRQTVGQQATGACLELATRYHDIAGTVGVARHNH

Sequences:

>Translated_602_residues
MTKITPGTARRKLRSSEWFNDPHNPAMTALYLERYLNYGLTRGELQSGKPIIGIAQTGNDLSPCNRHHLELAQRVREGIR
AAGGIAMEFPVHPIQETGKRPTAALDRNLAYLGLVEVLFSYPLDGVVLTTGCDKTTPACLMAAATVNIPAIVLSGGPMLN
GWHNGERSGSGTVVWKSRERLAAGEIDYEEFMEIVASSAPSVGHCNTMGTASTMNSLAEALGMSLPGCAAIPAPYRERGQ
IAYATGVRAVEMVWEDLKPSDILTREAFENAIVVNSAIGGSTNAPIHLNALARHIGVELSIDDWQSIGHKIPLLVNMQPA
GFYLGEEYHRAGGVPAVVRELMQHGKIHTDALTVNGLTMGQNCAGAPAPDGDVIKSYDGPLVQDAGFLVLRGNLFESAIM
KTSVISLEFRERYLSSPNDPNAFEGRAIVFEGPEDYHDRIDDPALKIDEHCILFVRGTGPIGYPGGAEVVNMQPPAALIK
RGIHSLPCIGDGRQSGTSGSPSILNATPEAAANGGLAILKTGDRVRIDLNTGSANILISDDELKQRRAELEAHGGFAYPK
HQTPWQELYRQTVGQQATGACLELATRYHDIAGTVGVARHNH
>Mature_601_residues
TKITPGTARRKLRSSEWFNDPHNPAMTALYLERYLNYGLTRGELQSGKPIIGIAQTGNDLSPCNRHHLELAQRVREGIRA
AGGIAMEFPVHPIQETGKRPTAALDRNLAYLGLVEVLFSYPLDGVVLTTGCDKTTPACLMAAATVNIPAIVLSGGPMLNG
WHNGERSGSGTVVWKSRERLAAGEIDYEEFMEIVASSAPSVGHCNTMGTASTMNSLAEALGMSLPGCAAIPAPYRERGQI
AYATGVRAVEMVWEDLKPSDILTREAFENAIVVNSAIGGSTNAPIHLNALARHIGVELSIDDWQSIGHKIPLLVNMQPAG
FYLGEEYHRAGGVPAVVRELMQHGKIHTDALTVNGLTMGQNCAGAPAPDGDVIKSYDGPLVQDAGFLVLRGNLFESAIMK
TSVISLEFRERYLSSPNDPNAFEGRAIVFEGPEDYHDRIDDPALKIDEHCILFVRGTGPIGYPGGAEVVNMQPPAALIKR
GIHSLPCIGDGRQSGTSGSPSILNATPEAAANGGLAILKTGDRVRIDLNTGSANILISDDELKQRRAELEAHGGFAYPKH
QTPWQELYRQTVGQQATGACLELATRYHDIAGTVGVARHNH

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=577, Percent_Identity=29.1161178509532, Blast_Score=202, Evalue=7e-53,
Organism=Escherichia coli, GI1788157, Length=456, Percent_Identity=30.9210526315789, Blast_Score=186, Evalue=5e-48,
Organism=Escherichia coli, GI2367371, Length=517, Percent_Identity=28.0464216634429, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1786464, Length=526, Percent_Identity=28.1368821292776, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322476, Length=512, Percent_Identity=30.2734375, Blast_Score=218, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 64635; Mature: 64504

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00886 ILVD_EDD_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKITPGTARRKLRSSEWFNDPHNPAMTALYLERYLNYGLTRGELQSGKPIIGIAQTGND
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
LSPCNRHHLELAQRVREGIRAAGGIAMEFPVHPIQETGKRPTAALDRNLAYLGLVEVLFS
CCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCCCCCHHHHCCHHHHHHHHHHHH
YPLDGVVLTTGCDKTTPACLMAAATVNIPAIVLSGGPMLNGWHNGERSGSGTVVWKSRER
CCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCEEEECCCCC
LAAGEIDYEEFMEIVASSAPSVGHCNTMGTASTMNSLAEALGMSLPGCAAIPAPYRERGQ
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
IAYATGVRAVEMVWEDLKPSDILTREAFENAIVVNSAIGGSTNAPIHLNALARHIGVELS
EEEECCCHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEHHHHHHHHCCEEE
IDDWQSIGHKIPLLVNMQPAGFYLGEEYHRAGGVPAVVRELMQHGKIHTDALTVNGLTMG
EHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCEEEC
QNCAGAPAPDGDVIKSYDGPLVQDAGFLVLRGNLFESAIMKTSVISLEFRERYLSSPNDP
CCCCCCCCCCCCCEECCCCCEEECCCEEEEECCHHHHHHHHHHHEEHHHHHHHCCCCCCC
NAFEGRAIVFEGPEDYHDRIDDPALKIDEHCILFVRGTGPIGYPGGAEVVNMQPPAALIK
CCCCCCEEEEECCHHHHHCCCCCCEEECCEEEEEEECCCCCCCCCCCCEEECCCCHHHHH
RGIHSLPCIGDGRQSGTSGSPSILNATPEAAANGGLAILKTGDRVRIDLNTGSANILISD
CCHHCCCCCCCCCCCCCCCCCCEEECCCCHHCCCCEEEEECCCEEEEEEECCCEEEEECC
DELKQRRAELEAHGGFAYPKHQTPWQELYRQTVGQQATGACLELATRYHDIAGTVGVARH
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHCC
NH
CC
>Mature Secondary Structure 
TKITPGTARRKLRSSEWFNDPHNPAMTALYLERYLNYGLTRGELQSGKPIIGIAQTGND
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
LSPCNRHHLELAQRVREGIRAAGGIAMEFPVHPIQETGKRPTAALDRNLAYLGLVEVLFS
CCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHCCCCCCHHHHCCHHHHHHHHHHHH
YPLDGVVLTTGCDKTTPACLMAAATVNIPAIVLSGGPMLNGWHNGERSGSGTVVWKSRER
CCCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCCCEEEECCCCC
LAAGEIDYEEFMEIVASSAPSVGHCNTMGTASTMNSLAEALGMSLPGCAAIPAPYRERGQ
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHCCC
IAYATGVRAVEMVWEDLKPSDILTREAFENAIVVNSAIGGSTNAPIHLNALARHIGVELS
EEEECCCHHHHHHHHHCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEHHHHHHHHCCEEE
IDDWQSIGHKIPLLVNMQPAGFYLGEEYHRAGGVPAVVRELMQHGKIHTDALTVNGLTMG
EHHHHHCCCCCCEEEEECCCCCCCCHHHHHCCCCHHHHHHHHHCCCEEEEEEEECCEEEC
QNCAGAPAPDGDVIKSYDGPLVQDAGFLVLRGNLFESAIMKTSVISLEFRERYLSSPNDP
CCCCCCCCCCCCCEECCCCCEEECCCEEEEECCHHHHHHHHHHHEEHHHHHHHCCCCCCC
NAFEGRAIVFEGPEDYHDRIDDPALKIDEHCILFVRGTGPIGYPGGAEVVNMQPPAALIK
CCCCCCEEEEECCHHHHHCCCCCCEEECCEEEEEEECCCCCCCCCCCCEEECCCCHHHHH
RGIHSLPCIGDGRQSGTSGSPSILNATPEAAANGGLAILKTGDRVRIDLNTGSANILISD
CCHHCCCCCCCCCCCCCCCCCCEEECCCCHHCCCCEEEEECCCEEEEEEECCCEEEEECC
DELKQRRAELEAHGGFAYPKHQTPWQELYRQTVGQQATGACLELATRYHDIAGTVGVARH
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCHHCC
NH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA