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The map label for this gene is tam

Identifier: 86749045

GI number: 86749045

Start: 2195051

End: 2195821

Strand: Direct

Name: tam

Synonym: RPB_1922

Alternate gene names: 86749045

Gene position: 2195051-2195821 (Clockwise)

Preceding gene: 86749042

Following gene: 86749046

Centisome position: 41.17

GC content: 65.5

Gene sequence:

>771_bases
ATGGCCGACTGGAGTGCCGAGCAATATCTGAAATTCGAGGACGAGCGCACCCGCCCGGCGCGTGAGTTGCTGGCGCAGAT
CCCTGTGGTCGCGCCCGGTAAGGTCGCCGATCTCGGTTGCGGGCCGGGCAATTCGACGGAGCTCCTGGTCGAGCGTTGGC
CCGATGCGTCCGTGATCGGCGTGGATACCTCCGCCGACATGCTGCGCCAGGCCCGCGAGCGGCTGCCGCAGCAAAAGTTC
ATCGAGGCCAATGTGGCGCATTGGGCGCCGCCGCCGGGCACCGAGGTGCTGTTCGCCAACGCGGTGTTCCAGTGGGTGCC
GGATCATCTGAAACACCTCAAGCGGCTGATCACGGGACTCGACGAAGGCGGCGTGCTCGCAGTCCAGATGCCCGACAATG
TCGACGAGCCGTCGCACGTGATGATGCGCGAGGTCGCATTTCAGGAGCCGTGGCGGCACCAGCTCTCTCAGGCCGCCGAG
CTTCGCGACCTGCTGCCGAAACCGGGCGCCTATTACGACGCCCTGCGGCCGCTGTGCAGCCGTCTGGATATCTGGCACAC
GGTCTACAACCACGTGCTCGACGACGCTGCGGCGATCGTTGAATGGGTCAAGGGCACCGGCCTGCGCCCGTTCATCGACC
CGCTCGACCTGCACGAGCGCAAGGCGTATCTGGCCGCCTATACCGCGCGGGTCGCGGCCGCTTATCCGCCGCAGTCCGAC
GGCAAAGTTCTGTTACGCTTCCCGCGTATTTTCATCGTCGCGATCAAGTAA

Upstream 100 bases:

>100_bases
TCGGCTTTCCAAGGGGGCCTGGGCAGATGCGCAGCCTCAGTTGCAAAATCCCGCCGGATCAATAGGTTTCCAGCCACGCG
GGGGCGCGAGGGTGGAGTGG

Downstream 100 bases:

>100_bases
CGGCCCGCGCATTCGCGAATCGCCAGCTGCGGTTAATCGCCGCTCCCCGGCGTCTCGACAGAATCGCACGGAGTTCCCAC
TTGAGAGGTCCCCGATTTCC

Product: trans-aconitate 2-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MADWSAEQYLKFEDERTRPARELLAQIPVVAPGKVADLGCGPGNSTELLVERWPDASVIGVDTSADMLRQARERLPQQKF
IEANVAHWAPPPGTEVLFANAVFQWVPDHLKHLKRLITGLDEGGVLAVQMPDNVDEPSHVMMREVAFQEPWRHQLSQAAE
LRDLLPKPGAYYDALRPLCSRLDIWHTVYNHVLDDAAAIVEWVKGTGLRPFIDPLDLHERKAYLAAYTARVAAAYPPQSD
GKVLLRFPRIFIVAIK

Sequences:

>Translated_256_residues
MADWSAEQYLKFEDERTRPARELLAQIPVVAPGKVADLGCGPGNSTELLVERWPDASVIGVDTSADMLRQARERLPQQKF
IEANVAHWAPPPGTEVLFANAVFQWVPDHLKHLKRLITGLDEGGVLAVQMPDNVDEPSHVMMREVAFQEPWRHQLSQAAE
LRDLLPKPGAYYDALRPLCSRLDIWHTVYNHVLDDAAAIVEWVKGTGLRPFIDPLDLHERKAYLAAYTARVAAAYPPQSD
GKVLLRFPRIFIVAIK
>Mature_255_residues
ADWSAEQYLKFEDERTRPARELLAQIPVVAPGKVADLGCGPGNSTELLVERWPDASVIGVDTSADMLRQARERLPQQKFI
EANVAHWAPPPGTEVLFANAVFQWVPDHLKHLKRLITGLDEGGVLAVQMPDNVDEPSHVMMREVAFQEPWRHQLSQAAEL
RDLLPKPGAYYDALRPLCSRLDIWHTVYNHVLDDAAAIVEWVKGTGLRPFIDPLDLHERKAYLAAYTARVAAAYPPQSDG
KVLLRFPRIFIVAIK

Specific function: Catalyzes the S-adenosylmethionine monomethyl esterification of trans-aconitate

COG id: COG4106

COG function: function code R; Trans-aconitate methyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. Tam family

Homologues:

Organism=Escherichia coli, GI1787798, Length=254, Percent_Identity=45.6692913385827, Blast_Score=230, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TAM_RHOP2 (Q2IYT0)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_485541.1
- ProteinModelPortal:   Q2IYT0
- SMR:   Q2IYT0
- STRING:   Q2IYT0
- GeneID:   3908001
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_1922
- eggNOG:   COG4106
- HOGENOM:   HBG328684
- OMA:   WETTYVH
- ProtClustDB:   PRK01683
- BioCyc:   RPAL316058:RPB_1922-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00560
- InterPro:   IPR013216
- InterPro:   IPR023149

Pfam domain/function: PF08241 Methyltransf_11

EC number: =2.1.1.144

Molecular weight: Translated: 28782; Mature: 28651

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADWSAEQYLKFEDERTRPARELLAQIPVVAPGKVADLGCGPGNSTELLVERWPDASVIG
CCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCEEE
VDTSADMLRQARERLPQQKFIEANVAHWAPPPGTEVLFANAVFQWVPDHLKHLKRLITGL
CCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DEGGVLAVQMPDNVDEPSHVMMREVAFQEPWRHQLSQAAELRDLLPKPGAYYDALRPLCS
CCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RLDIWHTVYNHVLDDAAAIVEWVKGTGLRPFIDPLDLHERKAYLAAYTARVAAAYPPQSD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GKVLLRFPRIFIVAIK
CCEEEEECEEEEEEEC
>Mature Secondary Structure 
ADWSAEQYLKFEDERTRPARELLAQIPVVAPGKVADLGCGPGNSTELLVERWPDASVIG
CCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCEEE
VDTSADMLRQARERLPQQKFIEANVAHWAPPPGTEVLFANAVFQWVPDHLKHLKRLITGL
CCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DEGGVLAVQMPDNVDEPSHVMMREVAFQEPWRHQLSQAAELRDLLPKPGAYYDALRPLCS
CCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
RLDIWHTVYNHVLDDAAAIVEWVKGTGLRPFIDPLDLHERKAYLAAYTARVAAAYPPQSD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GKVLLRFPRIFIVAIK
CCEEEEECEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA