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The map label for this gene is nasD [H]

Identifier: 86748878

GI number: 86748878

Start: 2008826

End: 2010058

Strand: Reverse

Name: nasD [H]

Synonym: RPB_1755

Alternate gene names: 86748878

Gene position: 2010058-2008826 (Counterclockwise)

Preceding gene: 86748879

Following gene: 86748877

Centisome position: 37.7

GC content: 69.75

Gene sequence:

>1233_bases
ATGAGCGAGCCGCTCGTCATCATCGGCAACGGCATGGCGGCGGTGCGGCTGGTCGAGGAACTCGCCAAGACCGCGCTCGG
CCGCTACGCCATCGCGGTGATCGGCGACGAGCCGCGGCTCGCCTACAACCGCGTGCTGTTGTCGTCGGTGCTGGCGCGCG
AAATCCCGGCCACCGAGATCGAGCTGCGGCCGGCGCAATGGTGGCGCGACCGCGGCGTCACGCTGCTGTACGGCGAGAGC
GCCGCAGCGAAGGCGATCGATCCCGCGATCCGCCGGGTGCGGCTCGCCACCGGCGCGACGCTACCGTTCTCCCGACTGGT
GATCGCCACCGGCTCGAAGCCGATCCGGCTGAACGTGCCCGGCATGGAGCTGCCGGGCGTGCTCACCTTCCGCGATCTCG
CCGACGTCGCGGCGATCGAACAGGCCGCCTCCGGTGGCCGCAGCGCCGTGGTGATCGGCGGCGGATTGCTCGGGCTCGAG
GCCGCCTACGGCCTCGCCAAATCCGGCACAAAGGTCACCGTGGTACACCTGATGGACCGGCTGATGGAGCGCCAGCTCGA
TCCGACCGCCGCCGCGATGCTCAAGCGCGCGGTCGAGCGCAAGGGCATCACTGTCGAACTCTCCGCCGACACCGCGGCGA
TCATCGGCGACGGCCGCGTCGAGGCGGTGCAGCTCGCCGACGGCCGCACCCTCGCCGCCGATCTCGTCGTGGTCGCCGCC
GGCATCCGCCCCAATGTCGAACTGGCGCGCGCCGCCGGCCTCGACATCGGCCGCGGCATTCTGGTCGACGATCATCTGCA
AACCAGCAAGCGCGACATCTACGCGATCGGCGAATGCGCCGAACATCGCGGCGCTTGCTACGGCCTGGTCGAGCCGGCTT
ACGAGCAGGCGCGCGCACTGGCCTCGCATCTCGCGGGCCGCGACGGCGCCTATCGCGGCAGCGTGCTGGCGACCAATCTC
AAAGTGTCGGGCGTCGATCTGTTCTCGGCCGGCGATTTCCTCGGCAGCCCGGGCACCGAGGAGATCGTGCTCACCGACCC
GGGCCTCGGCAGCTACAAGAAGCTGGTGATCAAGGGAGACAGGCTGACCGGCTGCGTGCTGTTCGGCGATACGGCCGACG
GGCTCTGGTATCTCGACCTGATCCGCTCCGGCCAATCGATCGACGCCATTCGCCACGACATGATGTTCGGCCGTGCGCTC
GCTGCGCGCGCCGGCAAATCCGTTGCCGCTTGA

Upstream 100 bases:

>100_bases
GACGCCGGCGCCTTGCGCGTGGCACCCCTCATCCGGCGCGGACGGTGTCCGCGCCACCTTCTCCCACAAGGGGAGAAGGA
AGGATTGGCGAGGCCCGCCG

Downstream 100 bases:

>100_bases
GGACCTGATGGGAAGCGAATTCACGATCGACCAGAAGCGATACCTGGAAGGCTTCGCCACCGGCATCAACGCCGCGCGGG
TGCAGCGCGGCGCCCTGCCC

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSEPLVIIGNGMAAVRLVEELAKTALGRYAIAVIGDEPRLAYNRVLLSSVLAREIPATEIELRPAQWWRDRGVTLLYGES
AAAKAIDPAIRRVRLATGATLPFSRLVIATGSKPIRLNVPGMELPGVLTFRDLADVAAIEQAASGGRSAVVIGGGLLGLE
AAYGLAKSGTKVTVVHLMDRLMERQLDPTAAAMLKRAVERKGITVELSADTAAIIGDGRVEAVQLADGRTLAADLVVVAA
GIRPNVELARAAGLDIGRGILVDDHLQTSKRDIYAIGECAEHRGACYGLVEPAYEQARALASHLAGRDGAYRGSVLATNL
KVSGVDLFSAGDFLGSPGTEEIVLTDPGLGSYKKLVIKGDRLTGCVLFGDTADGLWYLDLIRSGQSIDAIRHDMMFGRAL
AARAGKSVAA

Sequences:

>Translated_410_residues
MSEPLVIIGNGMAAVRLVEELAKTALGRYAIAVIGDEPRLAYNRVLLSSVLAREIPATEIELRPAQWWRDRGVTLLYGES
AAAKAIDPAIRRVRLATGATLPFSRLVIATGSKPIRLNVPGMELPGVLTFRDLADVAAIEQAASGGRSAVVIGGGLLGLE
AAYGLAKSGTKVTVVHLMDRLMERQLDPTAAAMLKRAVERKGITVELSADTAAIIGDGRVEAVQLADGRTLAADLVVVAA
GIRPNVELARAAGLDIGRGILVDDHLQTSKRDIYAIGECAEHRGACYGLVEPAYEQARALASHLAGRDGAYRGSVLATNL
KVSGVDLFSAGDFLGSPGTEEIVLTDPGLGSYKKLVIKGDRLTGCVLFGDTADGLWYLDLIRSGQSIDAIRHDMMFGRAL
AARAGKSVAA
>Mature_409_residues
SEPLVIIGNGMAAVRLVEELAKTALGRYAIAVIGDEPRLAYNRVLLSSVLAREIPATEIELRPAQWWRDRGVTLLYGESA
AAKAIDPAIRRVRLATGATLPFSRLVIATGSKPIRLNVPGMELPGVLTFRDLADVAAIEQAASGGRSAVVIGGGLLGLEA
AYGLAKSGTKVTVVHLMDRLMERQLDPTAAAMLKRAVERKGITVELSADTAAIIGDGRVEAVQLADGRTLAADLVVVAAG
IRPNVELARAAGLDIGRGILVDDHLQTSKRDIYAIGECAEHRGACYGLVEPAYEQARALASHLAGRDGAYRGSVLATNLK
VSGVDLFSAGDFLGSPGTEEIVLTDPGLGSYKKLVIKGDRLTGCVLFGDTADGLWYLDLIRSGQSIDAIRHDMMFGRALA
ARAGKSVAA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=280, Percent_Identity=27.5, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI226437568, Length=280, Percent_Identity=27.5, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI65787454, Length=280, Percent_Identity=27.5, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI22202629, Length=286, Percent_Identity=27.2727272727273, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI4757732, Length=286, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1789765, Length=378, Percent_Identity=37.8306878306878, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1789065, Length=333, Percent_Identity=29.4294294294294, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788892, Length=281, Percent_Identity=30.6049822064057, Blast_Score=106, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=404, Percent_Identity=26.7326732673267, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564386, Length=312, Percent_Identity=29.8076923076923, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585130, Length=282, Percent_Identity=24.822695035461, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI281359715, Length=364, Percent_Identity=26.0989010989011, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI281359713, Length=364, Percent_Identity=26.0989010989011, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24639250, Length=364, Percent_Identity=26.0989010989011, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18543267, Length=364, Percent_Identity=26.0989010989011, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24639252, Length=364, Percent_Identity=26.0989010989011, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24639257, Length=364, Percent_Identity=26.0989010989011, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24581020, Length=313, Percent_Identity=26.1980830670927, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI28573993, Length=313, Percent_Identity=26.1980830670927, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 43152; Mature: 43021

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPLVIIGNGMAAVRLVEELAKTALGRYAIAVIGDEPRLAYNRVLLSSVLAREIPATEI
CCCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEE
ELRPAQWWRDRGVTLLYGESAAAKAIDPAIRRVRLATGATLPFSRLVIATGSKPIRLNVP
EECHHHHHHHCCCEEEECCCCHHHHHHHHHHHEEHHCCCCCCCCEEEEEECCCEEEEECC
GMELPGVLTFRDLADVAAIEQAASGGRSAVVIGGGLLGLEAAYGLAKSGTKVTVVHLMDR
CCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEHHHHHHH
LMERQLDPTAAAMLKRAVERKGITVELSADTAAIIGDGRVEAVQLADGRTLAADLVVVAA
HHHHCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEEECCCCCHHEEEEEEEE
GIRPNVELARAAGLDIGRGILVDDHLQTSKRDIYAIGECAEHRGACYGLVEPAYEQARAL
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCEEEECHHHHHCCCEEECCCHHHHHHHHH
ASHLAGRDGAYRGSVLATNLKVSGVDLFSAGDFLGSPGTEEIVLTDPGLGSYKKLVIKGD
HHHHCCCCCCCCCCEEEEEEEEECEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEECC
RLTGCVLFGDTADGLWYLDLIRSGQSIDAIRHDMMFGRALAARAGKSVAA
CEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEPLVIIGNGMAAVRLVEELAKTALGRYAIAVIGDEPRLAYNRVLLSSVLAREIPATEI
CCCEEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEE
ELRPAQWWRDRGVTLLYGESAAAKAIDPAIRRVRLATGATLPFSRLVIATGSKPIRLNVP
EECHHHHHHHCCCEEEECCCCHHHHHHHHHHHEEHHCCCCCCCCEEEEEECCCEEEEECC
GMELPGVLTFRDLADVAAIEQAASGGRSAVVIGGGLLGLEAAYGLAKSGTKVTVVHLMDR
CCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCEEEHHHHHHH
LMERQLDPTAAAMLKRAVERKGITVELSADTAAIIGDGRVEAVQLADGRTLAADLVVVAA
HHHHCCCHHHHHHHHHHHHHCCCEEEECCCEEEEEECCCEEEEEECCCCCHHEEEEEEEE
GIRPNVELARAAGLDIGRGILVDDHLQTSKRDIYAIGECAEHRGACYGLVEPAYEQARAL
CCCCCCHHHHHCCCCCCCCEEEECCCCCCCCCEEEECHHHHHCCCEEECCCHHHHHHHHH
ASHLAGRDGAYRGSVLATNLKVSGVDLFSAGDFLGSPGTEEIVLTDPGLGSYKKLVIKGD
HHHHCCCCCCCCCCEEEEEEEEECEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEEECC
RLTGCVLFGDTADGLWYLDLIRSGQSIDAIRHDMMFGRALAARAGKSVAA
CEEEEEEECCCCCCEEEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]