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The map label for this gene is nirA [H]

Identifier: 86748877

GI number: 86748877

Start: 2007058

End: 2008818

Strand: Reverse

Name: nirA [H]

Synonym: RPB_1754

Alternate gene names: 86748877

Gene position: 2008818-2007058 (Counterclockwise)

Preceding gene: 86748878

Following gene: 86748876

Centisome position: 37.68

GC content: 67.58

Gene sequence:

>1761_bases
ATGGGAAGCGAATTCACGATCGACCAGAAGCGATACCTGGAAGGCTTCGCCACCGGCATCAACGCCGCGCGGGTGCAGCG
CGGCGCCCTGCCCGCCGCCGGCAGCGCGCAGCCCTCCGGCCCCGACGCGATCCACATCGCCGCGCAGGATCGCTACACTG
CCGCGGGCAAGAAACTCGCCGAAGCGGAGAAGTGGAAGCGCGAGGAGCATCCGTTCGACGCCTATGCGCGGCTGAAGCAG
CAGGCCAAGACAAATACGCCGCCGAAGCCGGCCGACAATTTCCGCTGGCGCTTTTACGGGCTGTTCTACGTCGCCCCGAC
CCAGAGCTCCTATATGTGCCGGTTGCGGATTCCCAATGGCGTGCTGACGTCGTGGCAGATGCAGGGCCTCGCCGACATCG
CCGACAATTGTGCCGGCGGCTATTCCCACGTGACGACGCGCGCCAATCTGCAGATGCGCGAGATCGCGCCGAAGAACGCC
GTGACTCTGATCGAAGGCATCGAGTCGCTCGGCCTGTGGGCGCGCGGCGCCGGCGCCGACAACATCCGCAACGTCACCGG
TTCGGCCACCGCCGGCATCGATCCGCAGGAATTGCTCGACACCCGCGCTTACGCGCGCGAGTGGCACTTCCACATCCTCA
ACGAGCGCGCGCTGTACGGCCTGCCGCGCAAATTCAACGTCGCCTTCGACGGTGGCGGGCTGATCCCGACGCTGGAGGAC
ACCAACGACATCGCGTTCCAGGCGGTGACGATCGGCGACGGCCACGGCGTCGAACCGGGCGTGTGGTTCCGGCTCTCGCT
CGGCGGCATCACCGGCCACAAGGATTTTGCCCGCGACACCGGCGTGATCGTGCCGCCGGATGAAGCGACCGAAGTCTCGG
ACGCGATCGTGCGGGTGTTCATCGAGCACGGCGACCGCACCGACCGGGCCAGGTCGCGGCTGAAATACGTGCTCGACCGT
TTCGGCTTCGATGAGTTTCTCAGGCGGGTCGAGGGGCGGCTCGGACGCAAGCTGGTGCGCGTGCCCGCGGAGGCCGCGCA
GCCACGGCCGGCGCAGGATCGCGCCGCGCATATCGGCGTCCATCGCCAGAAGCAGGCCGGGCTGAACTGGATCGGCGTGC
GGCTGCCGCTCGGCAAGCTGACGAGCGCGCAGATGCGCGGCCTCGCCGAGATTGCGCACAATTTCGGCGACGGCGATATC
CGGCTGACCGTGTGGCAGAACCTGTTGATCTCCGGCGTTCCGGACGCGCGTGTCGCGGACGCAAGTGCGGCGATCGTCGC
GCTCGGCCTCGCGATCGACGCCAGTCCGATCCGCGCCGGACTGATCGCCTGCACTGGCGCCACCGGCTGCCGGTTCGGCG
CCGCCAAGACCAAGGAAACCGCCGAAGCCATCGCGGGCTATTGCGAGCCGCGGGTGCCACTCGACACGCCGGTGAACATC
CACCTCACCGGCTGCCATCATTCCTGCGCGCAACATTACATCAGCGACATCGGCCTGATCGGCGCCAAGGTGGCGATCTC
CGACGAAGACACCGTCGAGGGCTTCCACATCCATGTCGGCGGTGCGTTCGGCGAAGGCGCCGCGATCGGCGCCGAAGTGC
TGCGCGACGTCAGACAGGACGACGCGCCGCGTGTCATCGCGCAAATGCTGAGCACGTATCTCGCGCAACGCGCTTCGCCG
GACGAAACCTTCCTCGCCTTCGCGCGCCGCCACGACACCCCGACGCTGCAACGTCTGTTCGCTCTGGAGACCGGTGCATG
A

Upstream 100 bases:

>100_bases
ATCCGCTCCGGCCAATCGATCGACGCCATTCGCCACGACATGATGTTCGGCCGTGCGCTCGCTGCGCGCGCCGGCAAATC
CGTTGCCGCTTGAGGACCTG

Downstream 100 bases:

>100_bases
GCCAGAATATGCCGCCGCCGATCCCCATGCTGGTCCCGGAGACCGCGCCGTTCTCCGACGAGCAGCGCGCCTGGCTGAAC
GGTTTCTTCGCCGGCCTCGT

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MGSEFTIDQKRYLEGFATGINAARVQRGALPAAGSAQPSGPDAIHIAAQDRYTAAGKKLAEAEKWKREEHPFDAYARLKQ
QAKTNTPPKPADNFRWRFYGLFYVAPTQSSYMCRLRIPNGVLTSWQMQGLADIADNCAGGYSHVTTRANLQMREIAPKNA
VTLIEGIESLGLWARGAGADNIRNVTGSATAGIDPQELLDTRAYAREWHFHILNERALYGLPRKFNVAFDGGGLIPTLED
TNDIAFQAVTIGDGHGVEPGVWFRLSLGGITGHKDFARDTGVIVPPDEATEVSDAIVRVFIEHGDRTDRARSRLKYVLDR
FGFDEFLRRVEGRLGRKLVRVPAEAAQPRPAQDRAAHIGVHRQKQAGLNWIGVRLPLGKLTSAQMRGLAEIAHNFGDGDI
RLTVWQNLLISGVPDARVADASAAIVALGLAIDASPIRAGLIACTGATGCRFGAAKTKETAEAIAGYCEPRVPLDTPVNI
HLTGCHHSCAQHYISDIGLIGAKVAISDEDTVEGFHIHVGGAFGEGAAIGAEVLRDVRQDDAPRVIAQMLSTYLAQRASP
DETFLAFARRHDTPTLQRLFALETGA

Sequences:

>Translated_586_residues
MGSEFTIDQKRYLEGFATGINAARVQRGALPAAGSAQPSGPDAIHIAAQDRYTAAGKKLAEAEKWKREEHPFDAYARLKQ
QAKTNTPPKPADNFRWRFYGLFYVAPTQSSYMCRLRIPNGVLTSWQMQGLADIADNCAGGYSHVTTRANLQMREIAPKNA
VTLIEGIESLGLWARGAGADNIRNVTGSATAGIDPQELLDTRAYAREWHFHILNERALYGLPRKFNVAFDGGGLIPTLED
TNDIAFQAVTIGDGHGVEPGVWFRLSLGGITGHKDFARDTGVIVPPDEATEVSDAIVRVFIEHGDRTDRARSRLKYVLDR
FGFDEFLRRVEGRLGRKLVRVPAEAAQPRPAQDRAAHIGVHRQKQAGLNWIGVRLPLGKLTSAQMRGLAEIAHNFGDGDI
RLTVWQNLLISGVPDARVADASAAIVALGLAIDASPIRAGLIACTGATGCRFGAAKTKETAEAIAGYCEPRVPLDTPVNI
HLTGCHHSCAQHYISDIGLIGAKVAISDEDTVEGFHIHVGGAFGEGAAIGAEVLRDVRQDDAPRVIAQMLSTYLAQRASP
DETFLAFARRHDTPTLQRLFALETGA
>Mature_585_residues
GSEFTIDQKRYLEGFATGINAARVQRGALPAAGSAQPSGPDAIHIAAQDRYTAAGKKLAEAEKWKREEHPFDAYARLKQQ
AKTNTPPKPADNFRWRFYGLFYVAPTQSSYMCRLRIPNGVLTSWQMQGLADIADNCAGGYSHVTTRANLQMREIAPKNAV
TLIEGIESLGLWARGAGADNIRNVTGSATAGIDPQELLDTRAYAREWHFHILNERALYGLPRKFNVAFDGGGLIPTLEDT
NDIAFQAVTIGDGHGVEPGVWFRLSLGGITGHKDFARDTGVIVPPDEATEVSDAIVRVFIEHGDRTDRARSRLKYVLDRF
GFDEFLRRVEGRLGRKLVRVPAEAAQPRPAQDRAAHIGVHRQKQAGLNWIGVRLPLGKLTSAQMRGLAEIAHNFGDGDIR
LTVWQNLLISGVPDARVADASAAIVALGLAIDASPIRAGLIACTGATGCRFGAAKTKETAEAIAGYCEPRVPLDTPVNIH
LTGCHHSCAQHYISDIGLIGAKVAISDEDTVEGFHIHVGGAFGEGAAIGAEVLRDVRQDDAPRVIAQMLSTYLAQRASPD
ETFLAFARRHDTPTLQRLFALETGA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=487, Percent_Identity=26.4887063655031, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6322597, Length=471, Percent_Identity=24.416135881104, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 63530; Mature: 63398

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSEFTIDQKRYLEGFATGINAARVQRGALPAAGSAQPSGPDAIHIAAQDRYTAAGKKLA
CCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
EAEKWKREEHPFDAYARLKQQAKTNTPPKPADNFRWRFYGLFYVAPTQSSYMCRLRIPNG
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCC
VLTSWQMQGLADIADNCAGGYSHVTTRANLQMREIAPKNAVTLIEGIESLGLWARGAGAD
CCCHHHHHHHHHHHHHCCCCCHHEEEECCEEEECCCCCCHHHHHHHHHHHCCEECCCCCC
NIRNVTGSATAGIDPQELLDTRAYAREWHFHILNERALYGLPRKFNVAFDGGGLIPTLED
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCHHHHCCCCEEEEEECCCCCCCCCCC
TNDIAFQAVTIGDGHGVEPGVWFRLSLGGITGHKDFARDTGVIVPPDEATEVSDAIVRVF
CCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHCCCCEEECCCCCHHHHHHHHHHH
IEHGDRTDRARSRLKYVLDRFGFDEFLRRVEGRLGRKLVRVPAEAAQPRPAQDRAAHIGV
HHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCCC
HRQKQAGLNWIGVRLPLGKLTSAQMRGLAEIAHNFGDGDIRLTVWQNLLISGVPDARVAD
CCHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHCC
ASAAIVALGLAIDASPIRAGLIACTGATGCRFGAAKTKETAEAIAGYCEPRVPLDTPVNI
CHHEEHHEEEHCCCCHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEE
HLTGCHHSCAQHYISDIGLIGAKVAISDEDTVEGFHIHVGGAFGEGAAIGAEVLRDVRQD
EEECCCHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
DAPRVIAQMLSTYLAQRASPDETFLAFARRHDTPTLQRLFALETGA
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
GSEFTIDQKRYLEGFATGINAARVQRGALPAAGSAQPSGPDAIHIAAQDRYTAAGKKLA
CCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHH
EAEKWKREEHPFDAYARLKQQAKTNTPPKPADNFRWRFYGLFYVAPTQSSYMCRLRIPNG
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCC
VLTSWQMQGLADIADNCAGGYSHVTTRANLQMREIAPKNAVTLIEGIESLGLWARGAGAD
CCCHHHHHHHHHHHHHCCCCCHHEEEECCEEEECCCCCCHHHHHHHHHHHCCEECCCCCC
NIRNVTGSATAGIDPQELLDTRAYAREWHFHILNERALYGLPRKFNVAFDGGGLIPTLED
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCHHHHCCCCEEEEEECCCCCCCCCCC
TNDIAFQAVTIGDGHGVEPGVWFRLSLGGITGHKDFARDTGVIVPPDEATEVSDAIVRVF
CCCEEEEEEEECCCCCCCCCEEEEEEECCCCCCCHHHCCCCEEECCCCCHHHHHHHHHHH
IEHGDRTDRARSRLKYVLDRFGFDEFLRRVEGRLGRKLVRVPAEAAQPRPAQDRAAHIGV
HHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHCCC
HRQKQAGLNWIGVRLPLGKLTSAQMRGLAEIAHNFGDGDIRLTVWQNLLISGVPDARVAD
CCHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCCCCCCHHCC
ASAAIVALGLAIDASPIRAGLIACTGATGCRFGAAKTKETAEAIAGYCEPRVPLDTPVNI
CHHEEHHEEEHCCCCHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEE
HLTGCHHSCAQHYISDIGLIGAKVAISDEDTVEGFHIHVGGAFGEGAAIGAEVLRDVRQD
EEECCCHHHHHHHHHHHHHHCEEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
DAPRVIAQMLSTYLAQRASPDETFLAFARRHDTPTLQRLFALETGA
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]