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The map label for this gene is paaF [C]

Identifier: 86748814

GI number: 86748814

Start: 1926625

End: 1927407

Strand: Reverse

Name: paaF [C]

Synonym: RPB_1691

Alternate gene names: 86748814

Gene position: 1927407-1926625 (Counterclockwise)

Preceding gene: 86748819

Following gene: 86748805

Centisome position: 36.15

GC content: 67.69

Gene sequence:

>783_bases
ATGTCGGACACGGAGAGCGCCACCACGCCGGTGCTCGGCATCGACGGCCCGCGCGCCACCATCCGGCTGAACCGGCCGAA
CCATCTCAACCGGCTGCAGCCGGACGACCTGGCGACGCTACTGGCACAATTCGCGCTGATCGACAGCGACCTCTCCATCC
GCGTGCTGGTCCTGACCGGCACCGGCCGGGCATTCTCGGCGGGATTCGACCTCGGCAAGGTCGCTGAACGCGCCGCAAGC
GAGGTTGAAAGCGAGTCGTCCGGTTCCGCGTTCGAAGTCGTCGCCAACCGGCTGGAGGATCTGCGGGTGCCGACGATCTG
CCGGCTCAACGGCGGCGTCTATGGCGGCTCGACCGACCTGGCGCTGGCCTGCGATTTCCGCATCGGCGTCGACACTGCCG
AGATGTTCATGCCGGCGGCCCGGCTCGGGCTGCACTACTACCCATCCGGGATCAAACGCTATGTCACCCGACTCGGCGTC
GACAATGCCAAGCGGCTGTTTCTCACCGCCCAGCGGATCGATGCCGCCGAAATGCTGCGGATCGGCTATCTGACGCAGAT
GGTGGCGATGGACCGGCTCGACCGCGAGGTCGACGCCCTCGCGGCAACCCTGGCCGGCAATGCGCCGAATGCGGTCGCCG
GCATGAAGCGCGCGCTCAACGAATTCGCGCGACAGGCGCTCGACGAGCAAGCGGCCGACGCCCGCGCCCGCGCCTCGATG
CGCGGCGACGAGATCAAAGAAGGCATCGCCGCGTACAAGGAGAAACGCGCAGCGCGGTTCTGA

Upstream 100 bases:

>100_bases
AGGGGTCGTCATGCCCGACGACACACCCCTCAGCCGGACTGTTTCCGGGAGCTTCCGTGTTCCCGACGCCGTCACCACGC
GAGCCCCTCGGAGACCACCC

Downstream 100 bases:

>100_bases
GGGGGGTGCGGATCACAATCTCTCAATTTGTCATTCCGGGTTCGCGCGGAGCCCATGATCGGGCCGGCCAAAGGCCGAGC
CGATGGCGCGCAACCGAATG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSDTESATTPVLGIDGPRATIRLNRPNHLNRLQPDDLATLLAQFALIDSDLSIRVLVLTGTGRAFSAGFDLGKVAERAAS
EVESESSGSAFEVVANRLEDLRVPTICRLNGGVYGGSTDLALACDFRIGVDTAEMFMPAARLGLHYYPSGIKRYVTRLGV
DNAKRLFLTAQRIDAAEMLRIGYLTQMVAMDRLDREVDALAATLAGNAPNAVAGMKRALNEFARQALDEQAADARARASM
RGDEIKEGIAAYKEKRAARF

Sequences:

>Translated_260_residues
MSDTESATTPVLGIDGPRATIRLNRPNHLNRLQPDDLATLLAQFALIDSDLSIRVLVLTGTGRAFSAGFDLGKVAERAAS
EVESESSGSAFEVVANRLEDLRVPTICRLNGGVYGGSTDLALACDFRIGVDTAEMFMPAARLGLHYYPSGIKRYVTRLGV
DNAKRLFLTAQRIDAAEMLRIGYLTQMVAMDRLDREVDALAATLAGNAPNAVAGMKRALNEFARQALDEQAADARARASM
RGDEIKEGIAAYKEKRAARF
>Mature_259_residues
SDTESATTPVLGIDGPRATIRLNRPNHLNRLQPDDLATLLAQFALIDSDLSIRVLVLTGTGRAFSAGFDLGKVAERAASE
VESESSGSAFEVVANRLEDLRVPTICRLNGGVYGGSTDLALACDFRIGVDTAEMFMPAARLGLHYYPSGIKRYVTRLGVD
NAKRLFLTAQRIDAAEMLRIGYLTQMVAMDRLDREVDALAATLAGNAPNAVAGMKRALNEFARQALDEQAADARARASMR
GDEIKEGIAAYKEKRAARF

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=242, Percent_Identity=27.6859504132231, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1787659, Length=242, Percent_Identity=27.6859504132231, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI221142681, Length=214, Percent_Identity=29.9065420560748, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=27.8409090909091, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787660, Length=245, Percent_Identity=29.7959183673469, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788597, Length=248, Percent_Identity=25.8064516129032, Blast_Score=64, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=27.8688524590164, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=244, Percent_Identity=29.0983606557377, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17534483, Length=230, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=173, Percent_Identity=26.5895953757225, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI20129971, Length=270, Percent_Identity=29.2592592592593, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24653477, Length=270, Percent_Identity=29.2592592592593, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19922422, Length=257, Percent_Identity=25.6809338521401, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650670, Length=186, Percent_Identity=30.6451612903226, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21357171, Length=168, Percent_Identity=26.1904761904762, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28122; Mature: 27991

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTESATTPVLGIDGPRATIRLNRPNHLNRLQPDDLATLLAQFALIDSDLSIRVLVLTG
CCCCCCCCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
TGRAFSAGFDLGKVAERAASEVESESSGSAFEVVANRLEDLRVPTICRLNGGVYGGSTDL
CCCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCE
ALACDFRIGVDTAEMFMPAARLGLHYYPSGIKRYVTRLGVDNAKRLFLTAQRIDAAEMLR
EEEEEEEECCCHHHHHHHHHHHCCHHCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
IGYLTQMVAMDRLDREVDALAATLAGNAPNAVAGMKRALNEFARQALDEQAADARARASM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGDEIKEGIAAYKEKRAARF
CHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDTESATTPVLGIDGPRATIRLNRPNHLNRLQPDDLATLLAQFALIDSDLSIRVLVLTG
CCCCCCCCCEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEC
TGRAFSAGFDLGKVAERAASEVESESSGSAFEVVANRLEDLRVPTICRLNGGVYGGSTDL
CCCCEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCEECCCCCE
ALACDFRIGVDTAEMFMPAARLGLHYYPSGIKRYVTRLGVDNAKRLFLTAQRIDAAEMLR
EEEEEEEECCCHHHHHHHHHHHCCHHCHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
IGYLTQMVAMDRLDREVDALAATLAGNAPNAVAGMKRALNEFARQALDEQAADARARASM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
RGDEIKEGIAAYKEKRAARF
CHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA