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The map label for this gene is rfaF [H]

Identifier: 86748726

GI number: 86748726

Start: 1804001

End: 1805077

Strand: Reverse

Name: rfaF [H]

Synonym: RPB_1601

Alternate gene names: 86748726

Gene position: 1805077-1804001 (Counterclockwise)

Preceding gene: 86748730

Following gene: 86748725

Centisome position: 33.86

GC content: 68.71

Gene sequence:

>1077_bases
ATGAATTACGATTCACTCAAAATGAGCCTGGCCGTAGCGGCTGACCGCGCGGAAACCAGCCCGGTGCTGCTGATCCCTTA
TATGTGGATCGGCGATTTCGTGCGCTGCCATACGGTCGTGCGGGTCCTGAAAGACCGCTGGCCGGACCGGCCGGTGGATG
TCCTGACCACGACGCTTTGCGCACCTTTGGTGGATTACATGCCCGGCGTGCGCCGGGGCGTCGTCTGGGACCTGCCGCGC
AAGCGATTGGCGCTCGACCAGCAGCGGGCGCTGGCGGCGAAGCTCCGCGAGCAGCACTACGGCGCCTCGCTGGTGATGCC
GCGGACGTTCAAATCCACGATTGCGCCGTTTCTCGCCGGTATCCCGAACCGCACCGGCTTCATCGGCGAGGTCCGGTTCG
GCCTGCTCAACGACTGGCGGCGCGGCGAGAAGGCGCTGCCGCGGATGATCGACCGTTGTGCCGCACTGGCGCTGCCGGCC
GGCATCGACCTGCCGATGGATTGGCCGGAGCCGCAACTCGTTGTGCCGCCAGCCGAGATCGCCGCCTGGCGGCGGGCCAA
CGGGCTGGAGGGCCGGACTGCCGTGGCGCTGGCGCCAGGCGCGGTCGGCCCGTCGAAGCGCTGGACCTATTATGCCGAGG
CGGCCAAAGCGCTGACCGACCGCGGTCTGGACGTCTGGGTGATCGGCGGCCCCGGCGAGAGCGAGAAGGCCGCCGAAATC
GTCGCCGCGGCCGGTCCACGCGCGCGAGACCTCACCGGCACGGACCTGCGCAACGGCATCATGGCGCTGGCGGCGGCCGA
TCTGGTGATCTCCAACGATTCCGGCCTGCTCCACGTCGCAGCAGCGATCGGCAGCCGCACCATCGGCATCTTCGGCCCGA
CCAGCGCCTGGCATTATGCGCCGCTCAACCCGATCGAGGCGGTGATCGAGACCAGGACCGACGTGCCCTGCCGCCCCTGC
CACAAACCGGTGTGCCGGATGGTACATCACAAATGCATGCGCGACATTCCGGTCGAGGATGTGATGGCGGCGGCGCAGCA
AGCGCTGGGCAAGGCCGGCCTCGCCCCGGCGCGATAG

Upstream 100 bases:

>100_bases
AGCGTCACGGCCGAAATCCCGGCTGGACGCCGGGCGGCGCAAGCGCTAACCGGCATCACTGCGTTCAACTCGGGGGTATC
AGCCTCCGCCAGACAGCGTA

Downstream 100 bases:

>100_bases
ACCGCGCGACTTGCACCCGCCCCTGCGCTGCGCTTAACCAGTGCCGACCCATCAGCTTTCAGGTGTGAATTGAGCCAGGA
CCGGATCGCCGCCCATTTCC

Product: lipopolysaccharide heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MNYDSLKMSLAVAADRAETSPVLLIPYMWIGDFVRCHTVVRVLKDRWPDRPVDVLTTTLCAPLVDYMPGVRRGVVWDLPR
KRLALDQQRALAAKLREQHYGASLVMPRTFKSTIAPFLAGIPNRTGFIGEVRFGLLNDWRRGEKALPRMIDRCAALALPA
GIDLPMDWPEPQLVVPPAEIAAWRRANGLEGRTAVALAPGAVGPSKRWTYYAEAAKALTDRGLDVWVIGGPGESEKAAEI
VAAAGPRARDLTGTDLRNGIMALAAADLVISNDSGLLHVAAAIGSRTIGIFGPTSAWHYAPLNPIEAVIETRTDVPCRPC
HKPVCRMVHHKCMRDIPVEDVMAAAQQALGKAGLAPAR

Sequences:

>Translated_358_residues
MNYDSLKMSLAVAADRAETSPVLLIPYMWIGDFVRCHTVVRVLKDRWPDRPVDVLTTTLCAPLVDYMPGVRRGVVWDLPR
KRLALDQQRALAAKLREQHYGASLVMPRTFKSTIAPFLAGIPNRTGFIGEVRFGLLNDWRRGEKALPRMIDRCAALALPA
GIDLPMDWPEPQLVVPPAEIAAWRRANGLEGRTAVALAPGAVGPSKRWTYYAEAAKALTDRGLDVWVIGGPGESEKAAEI
VAAAGPRARDLTGTDLRNGIMALAAADLVISNDSGLLHVAAAIGSRTIGIFGPTSAWHYAPLNPIEAVIETRTDVPCRPC
HKPVCRMVHHKCMRDIPVEDVMAAAQQALGKAGLAPAR
>Mature_358_residues
MNYDSLKMSLAVAADRAETSPVLLIPYMWIGDFVRCHTVVRVLKDRWPDRPVDVLTTTLCAPLVDYMPGVRRGVVWDLPR
KRLALDQQRALAAKLREQHYGASLVMPRTFKSTIAPFLAGIPNRTGFIGEVRFGLLNDWRRGEKALPRMIDRCAALALPA
GIDLPMDWPEPQLVVPPAEIAAWRRANGLEGRTAVALAPGAVGPSKRWTYYAEAAKALTDRGLDVWVIGGPGESEKAAEI
VAAAGPRARDLTGTDLRNGIMALAAADLVISNDSGLLHVAAAIGSRTIGIFGPTSAWHYAPLNPIEAVIETRTDVPCRPC
HKPVCRMVHHKCMRDIPVEDVMAAAQQALGKAGLAPAR

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=307, Percent_Identity=37.785016286645, Blast_Score=180, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 38890; Mature: 38890

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYDSLKMSLAVAADRAETSPVLLIPYMWIGDFVRCHTVVRVLKDRWPDRPVDVLTTTLC
CCCCHHEEEHEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
APLVDYMPGVRRGVVWDLPRKRLALDQQRALAAKLREQHYGASLVMPRTFKSTIAPFLAG
HHHHHHCCCHHCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHC
IPNRTGFIGEVRFGLLNDWRRGEKALPRMIDRCAALALPAGIDLPMDWPEPQLVVPPAEI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHH
AAWRRANGLEGRTAVALAPGAVGPSKRWTYYAEAAKALTDRGLDVWVIGGPGESEKAAEI
HHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
VAAAGPRARDLTGTDLRNGIMALAAADLVISNDSGLLHVAAAIGSRTIGIFGPTSAWHYA
HHHCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEHEEHHCCCEEEEECCCCCCCCC
PLNPIEAVIETRTDVPCRPCHKPVCRMVHHKCMRDIPVEDVMAAAQQALGKAGLAPAR
CCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNYDSLKMSLAVAADRAETSPVLLIPYMWIGDFVRCHTVVRVLKDRWPDRPVDVLTTTLC
CCCCHHEEEHEEECCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
APLVDYMPGVRRGVVWDLPRKRLALDQQRALAAKLREQHYGASLVMPRTFKSTIAPFLAG
HHHHHHCCCHHCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHC
IPNRTGFIGEVRFGLLNDWRRGEKALPRMIDRCAALALPAGIDLPMDWPEPQLVVPPAEI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECHHHH
AAWRRANGLEGRTAVALAPGAVGPSKRWTYYAEAAKALTDRGLDVWVIGGPGESEKAAEI
HHHHHCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
VAAAGPRARDLTGTDLRNGIMALAAADLVISNDSGLLHVAAAIGSRTIGIFGPTSAWHYA
HHHCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCEEHEEHHCCCEEEEECCCCCCCCC
PLNPIEAVIETRTDVPCRPCHKPVCRMVHHKCMRDIPVEDVMAAAQQALGKAGLAPAR
CCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]