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The map label for this gene is cysU [H]

Identifier: 86748171

GI number: 86748171

Start: 1203039

End: 1203965

Strand: Direct

Name: cysU [H]

Synonym: RPB_1046

Alternate gene names: 86748171

Gene position: 1203039-1203965 (Clockwise)

Preceding gene: 86748170

Following gene: 86748172

Centisome position: 22.56

GC content: 67.53

Gene sequence:

>927_bases
ATGCGCGTCGACCTCTCCCCGCAGGCGGGGAGAGGTGAGGTTGCGCTCGGCGAACGCTGTCGTTCGTCCGGCGCGCCGTC
GTGTGCTGCAGTGAGCGCGATCGCACGACGACGGTCGTTGCCGGGCTTCGGCCTCACCATGGGCCTGACCCTGATGTGGC
TGTCGCTGATGATCCTGATTCCGCTGGCCGGGCTGTTCGTGAAGACGGCCGAACTCAGCCTCTCCGAATTCTGGGCGACG
GTGACCGCGCCGCGCACGCTGCATGCCTTGAAGATCTCGTTCGGGCTGGCCTTCGCCGCCGCCTGCGTCAATCTGGTCGC
CGGCCTGCTGATCGTCTGGGCGCTGGTGCGTTACAGCTTCCCCGGCCGGCGGATCTTCGACGCCATCGTCGACATCCCGT
TCGCGCTGCCGACCGCGGTCGCCGGCATCGCGCTGACGACGCTGTTCGCGCAGAACGGCTGGCTCGGCGCGCCGCTCGCC
GAACTCGGCATCAAGGTGGCGTTCACGCCGCTCGGGATTTTTCTGGCGATGGTGTTCATCGGGATTCCGTTCGTGGTGCG
CACCGTGCAGCCGGTGCTGATCGATCTCGACGCCGAACTCGAGGAGGCCGCGGAATCGCTCGGGGCGTCGCGGCTACAGA
CCATCCGCCGCGTCATCCTGCCGTCGCTGACGCCGGCGGTGATGACCGGCTTCGCGCTCGCCTTCGCCCGCGCGGTCGGC
GAATACGGTTCGGTGATCTTCATCGCCGGCAACCTGCCCAACGTCTCGGAGATCGCGCCGCTGCTGATCGTGATCCGGCT
GGCGGAGTTCCGCTATGCCGACGCCACCGCGATCGCGGTGGTGATGCTGCTCTTCTCCTTCCTGATCATTCTCGTTCTGA
ACCGGCTGCAGCATTGGGCGCAGACCCGGTCGCTGGCGGGAGCGTGA

Upstream 100 bases:

>100_bases
TCCCCGCCCGCGGGGAGAGGGCCCGGGTGAGGGGGCGTTTCCACGAGTCTGAGCCGCTCGGCCGTGTGGAGACGCCCCCT
CACCCGCCGCGCATCTGGCG

Downstream 100 bases:

>100_bases
GGCGATGACGCTGGTCGCGACCACATCCGTGAGGCGCTCCCCGCGCAAGCCTGCCGTCGCGGCCGGCGCACGTGCCGGGC
AGCCGGCGCGGCGCCCGGCG

Product: sulfate ABC transporter, permease protein CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRVDLSPQAGRGEVALGERCRSSGAPSCAAVSAIARRRSLPGFGLTMGLTLMWLSLMILIPLAGLFVKTAELSLSEFWAT
VTAPRTLHALKISFGLAFAAACVNLVAGLLIVWALVRYSFPGRRIFDAIVDIPFALPTAVAGIALTTLFAQNGWLGAPLA
ELGIKVAFTPLGIFLAMVFIGIPFVVRTVQPVLIDLDAELEEAAESLGASRLQTIRRVILPSLTPAVMTGFALAFARAVG
EYGSVIFIAGNLPNVSEIAPLLIVIRLAEFRYADATAIAVVMLLFSFLIILVLNRLQHWAQTRSLAGA

Sequences:

>Translated_308_residues
MRVDLSPQAGRGEVALGERCRSSGAPSCAAVSAIARRRSLPGFGLTMGLTLMWLSLMILIPLAGLFVKTAELSLSEFWAT
VTAPRTLHALKISFGLAFAAACVNLVAGLLIVWALVRYSFPGRRIFDAIVDIPFALPTAVAGIALTTLFAQNGWLGAPLA
ELGIKVAFTPLGIFLAMVFIGIPFVVRTVQPVLIDLDAELEEAAESLGASRLQTIRRVILPSLTPAVMTGFALAFARAVG
EYGSVIFIAGNLPNVSEIAPLLIVIRLAEFRYADATAIAVVMLLFSFLIILVLNRLQHWAQTRSLAGA
>Mature_308_residues
MRVDLSPQAGRGEVALGERCRSSGAPSCAAVSAIARRRSLPGFGLTMGLTLMWLSLMILIPLAGLFVKTAELSLSEFWAT
VTAPRTLHALKISFGLAFAAACVNLVAGLLIVWALVRYSFPGRRIFDAIVDIPFALPTAVAGIALTTLFAQNGWLGAPLA
ELGIKVAFTPLGIFLAMVFIGIPFVVRTVQPVLIDLDAELEEAAESLGASRLQTIRRVILPSLTPAVMTGFALAFARAVG
EYGSVIFIAGNLPNVSEIAPLLIVIRLAEFRYADATAIAVVMLLFSFLIILVLNRLQHWAQTRSLAGA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=265, Percent_Identity=50.188679245283, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI87082099, Length=274, Percent_Identity=31.7518248175182, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786980, Length=174, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787369, Length=234, Percent_Identity=26.4957264957265, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786254, Length=214, Percent_Identity=25.2336448598131, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787368, Length=221, Percent_Identity=30.316742081448, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32829; Mature: 32829

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLSPQAGRGEVALGERCRSSGAPSCAAVSAIARRRSLPGFGLTMGLTLMWLSLMILI
CCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
PLAGLFVKTAELSLSEFWATVTAPRTLHALKISFGLAFAAACVNLVAGLLIVWALVRYSF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGRRIFDAIVDIPFALPTAVAGIALTTLFAQNGWLGAPLAELGIKVAFTPLGIFLAMVFI
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
GIPFVVRTVQPVLIDLDAELEEAAESLGASRLQTIRRVILPSLTPAVMTGFALAFARAVG
HHHHHHHHHHHHHEECCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EYGSVIFIAGNLPNVSEIAPLLIVIRLAEFRYADATAIAVVMLLFSFLIILVLNRLQHWA
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTRSLAGA
HHHHCCCC
>Mature Secondary Structure
MRVDLSPQAGRGEVALGERCRSSGAPSCAAVSAIARRRSLPGFGLTMGLTLMWLSLMILI
CCCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
PLAGLFVKTAELSLSEFWATVTAPRTLHALKISFGLAFAAACVNLVAGLLIVWALVRYSF
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PGRRIFDAIVDIPFALPTAVAGIALTTLFAQNGWLGAPLAELGIKVAFTPLGIFLAMVFI
CCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHHHHH
GIPFVVRTVQPVLIDLDAELEEAAESLGASRLQTIRRVILPSLTPAVMTGFALAFARAVG
HHHHHHHHHHHHHEECCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EYGSVIFIAGNLPNVSEIAPLLIVIRLAEFRYADATAIAVVMLLFSFLIILVLNRLQHWA
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTRSLAGA
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]