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The map label for this gene is sbp [H]

Identifier: 86748170

GI number: 86748170

Start: 1201886

End: 1202869

Strand: Direct

Name: sbp [H]

Synonym: RPB_1045

Alternate gene names: 86748170

Gene position: 1201886-1202869 (Clockwise)

Preceding gene: 86748169

Following gene: 86748171

Centisome position: 22.54

GC content: 62.8

Gene sequence:

>984_bases
ATGTTTCGCCGAATTGTTCCGCTGCTCGCCGGCCTGATGGTCGCGACCTCCGCGCAGGCCGCCGACGTGTCGCTGCTCAA
CGTCTCGTACGACCCGACCCGCGAACTCTACAGCGAGTTCAACAAATCCTTCGCCGCCGCGTATCAGAAGGAAACCGGCG
ACACCGTCACGATCAAGCAGTCGCACGGCGGCTCCGGCTCGCAGGCGCGCTCGGTGATCGACGGTCTGCAGGCCGACGTC
GTGACGCTGGCGCTGGCCTACGACATCGACGCGATCGCCAACAAGGGGCTGCTGACCAAGGACTGGCAGAAGCGTCTGCC
GCAGAATGCGTCGCCCTACACCTCGACCATCGTGTTCCTGGTGCGCAAGGGCAATCCGAAGGGCATCAAGGACTGGCACG
ATCTGATCAGGCCCGGAATCAGCGTGATCACGCCGAACCCGAAGACCTCCGGCGGCGCGCGCTGGAATTATCTGGCGGCC
TGGGGCTACGCGCTGAAGACGGAGGGATCGGAGGACAAGGCGCGCGACTTCGTCGGGAACATTTACAAGAATGTGCCGGT
GCTGGACACCGGCGCCCGCGGCGCGACCATGACCTTCGTCCAGCGTGGCGTCGGCGACGTGCTGCTGGCGTGGGAGAACG
AGGCATTCCTGGCGGTCAAGGAATTCGGCAAGGACAGATTCGAGATCGTGGTGCCGTCGATCTCGATTCGCGCCGAGCCG
CCGGTGGCGCTGGTCGACAGCGTGGTCGACAAGAAAGGTACCCGGGCAGTGGCCGAAGCCTATCTGCAGTATTGGTACAC
CAAGGAAGGTCAGGAAATCGCCGCACGGAACTTCTATCGTCCGCGCGATTCGGAGATTGCCAACAAGCACGCCTTCGCGA
AGGTCGAGTTGTTCACCATCGACGAATTGTTCGGCGGCTGGACCAAGGCGCAGACGACGCACTTCACCGACGGTGGGGTG
TTCGACAAGATCTACAAGAACTGA

Upstream 100 bases:

>100_bases
ACAAAGAAATGCCGTTTCATTGACTTGCGATGGTTTGTTGACGAGCTTCGTACCTCCTCCGCAGACGACGCGGATGTCGT
CCGGACAAAGGAGATTCGAG

Downstream 100 bases:

>100_bases
GCGACGCGATCGCCGTGCGATCGATTTTGGGGGATGATGTGCTTCGCAGCAATCACAGCGCGCTCCCTCTCCCCGCCCGC
GGGGAGAGGGCCCGGGTGAG

Product: thiosulphate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MFRRIVPLLAGLMVATSAQAADVSLLNVSYDPTRELYSEFNKSFAAAYQKETGDTVTIKQSHGGSGSQARSVIDGLQADV
VTLALAYDIDAIANKGLLTKDWQKRLPQNASPYTSTIVFLVRKGNPKGIKDWHDLIRPGISVITPNPKTSGGARWNYLAA
WGYALKTEGSEDKARDFVGNIYKNVPVLDTGARGATMTFVQRGVGDVLLAWENEAFLAVKEFGKDRFEIVVPSISIRAEP
PVALVDSVVDKKGTRAVAEAYLQYWYTKEGQEIAARNFYRPRDSEIANKHAFAKVELFTIDELFGGWTKAQTTHFTDGGV
FDKIYKN

Sequences:

>Translated_327_residues
MFRRIVPLLAGLMVATSAQAADVSLLNVSYDPTRELYSEFNKSFAAAYQKETGDTVTIKQSHGGSGSQARSVIDGLQADV
VTLALAYDIDAIANKGLLTKDWQKRLPQNASPYTSTIVFLVRKGNPKGIKDWHDLIRPGISVITPNPKTSGGARWNYLAA
WGYALKTEGSEDKARDFVGNIYKNVPVLDTGARGATMTFVQRGVGDVLLAWENEAFLAVKEFGKDRFEIVVPSISIRAEP
PVALVDSVVDKKGTRAVAEAYLQYWYTKEGQEIAARNFYRPRDSEIANKHAFAKVELFTIDELFGGWTKAQTTHFTDGGV
FDKIYKN
>Mature_327_residues
MFRRIVPLLAGLMVATSAQAADVSLLNVSYDPTRELYSEFNKSFAAAYQKETGDTVTIKQSHGGSGSQARSVIDGLQADV
VTLALAYDIDAIANKGLLTKDWQKRLPQNASPYTSTIVFLVRKGNPKGIKDWHDLIRPGISVITPNPKTSGGARWNYLAA
WGYALKTEGSEDKARDFVGNIYKNVPVLDTGARGATMTFVQRGVGDVLLAWENEAFLAVKEFGKDRFEIVVPSISIRAEP
PVALVDSVVDKKGTRAVAEAYLQYWYTKEGQEIAARNFYRPRDSEIANKHAFAKVELFTIDELFGGWTKAQTTHFTDGGV
FDKIYKN

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=321, Percent_Identity=68.5358255451713, Blast_Score=462, Evalue=1e-132,
Organism=Escherichia coli, GI1788765, Length=305, Percent_Identity=47.5409836065574, Blast_Score=282, Evalue=2e-77,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36097; Mature: 36097

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRRIVPLLAGLMVATSAQAADVSLLNVSYDPTRELYSEFNKSFAAAYQKETGDTVTIKQ
CHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
SHGGSGSQARSVIDGLQADVVTLALAYDIDAIANKGLLTKDWQKRLPQNASPYTSTIVFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEEE
VRKGNPKGIKDWHDLIRPGISVITPNPKTSGGARWNYLAAWGYALKTEGSEDKARDFVGN
EECCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCEEEHEEECEEEECCCCHHHHHHHHHH
IYKNVPVLDTGARGATMTFVQRGVGDVLLAWENEAFLAVKEFGKDRFEIVVPSISIRAEP
HHHCCCEEECCCCCHHHHHHHHCCCCEEEEECCCEEEEHHHCCCCEEEEEECCEEECCCC
PVALVDSVVDKKGTRAVAEAYLQYWYTKEGQEIAARNFYRPRDSEIANKHAFAKVELFTI
CHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCEEEEEEEEH
DELFGGWTKAQTTHFTDGGVFDKIYKN
HHHHCCCHHCCEEECCCCCHHHHHHCC
>Mature Secondary Structure
MFRRIVPLLAGLMVATSAQAADVSLLNVSYDPTRELYSEFNKSFAAAYQKETGDTVTIKQ
CHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
SHGGSGSQARSVIDGLQADVVTLALAYDIDAIANKGLLTKDWQKRLPQNASPYTSTIVFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEEEE
VRKGNPKGIKDWHDLIRPGISVITPNPKTSGGARWNYLAAWGYALKTEGSEDKARDFVGN
EECCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCEEEHEEECEEEECCCCHHHHHHHHHH
IYKNVPVLDTGARGATMTFVQRGVGDVLLAWENEAFLAVKEFGKDRFEIVVPSISIRAEP
HHHCCCEEECCCCCHHHHHHHHCCCCEEEEECCCEEEEHHHCCCCEEEEEECCEEECCCC
PVALVDSVVDKKGTRAVAEAYLQYWYTKEGQEIAARNFYRPRDSEIANKHAFAKVELFTI
CHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHCEEEEEEEEH
DELFGGWTKAQTTHFTDGGVFDKIYKN
HHHHCCCHHCCEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]