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The map label for this gene is cysH [H]

Identifier: 86748169

GI number: 86748169

Start: 1201027

End: 1201761

Strand: Direct

Name: cysH [H]

Synonym: RPB_1044

Alternate gene names: 86748169

Gene position: 1201027-1201761 (Clockwise)

Preceding gene: 86748162

Following gene: 86748170

Centisome position: 22.53

GC content: 68.3

Gene sequence:

>735_bases
ATGAGTGAACTGGCGCAGTCCGTGTCTTTGCCGTCGGCGAACCCGTCCGAGGCGCGTGCGCGCGACCTCGACGCGGCGCT
GCGCGCTGCGTCGCCGCCGGAGATCATCGCCGCGGCGCTGCGCGAGGTCGGCCGCGACAGGCTCGCGCTGGTGTCGTCGT
TCGGCACCGAATCGGCGGCGTTGCTGAAAGTGATGGCCGATGTCGATCCGGCGATCCCGGTGGTGTTTCTCGACACCGGC
TGGCTGTTCGCCGAGACGCTGGCCTATCGCGACACGCTGATCGATCAGCTCGGGCTCACCGACGTGCGCTCGATCAAGCC
GCTGGATGCCGATCTCGATCGCGACGATCCGGCGCGCGATCTGTGGTTCTCCGATCCGGACCAATGCTGCTTCATCCGCA
AGGTCGAGCCGCTGCGCCGCGCGCTGGCGCCGTTCGAGGCGTGGCTCAACGGCCGCAAGCGCTTCCAGGGCGGCGCCCGC
GCCGAGATCCCGGTGGTCGAAGCCGACGGCGTGCGGATGAAGTTCAATCCGTTCGCCAACGTTTCGCGCGAGGACATCAA
CGCGATCTATGCGCTCGGCAAGCTGCCGCCGCATCCGCTGCTGGCGTCGGGCTTTCTGTCGGTCGGCTGCATGCCGTGCA
CCAGCCGCGCCGAGTCGGACCAGGATACGCGCTCCGGCCGTTGGCAGGGCCGCGGCAAGACGGAATGCGGCATTCACACG
ATGAAGACGTCGTAG

Upstream 100 bases:

>100_bases
TAGAAAATAATTCTAGTCAATCCTCCTTCGGATCATTCGGCGCCGGCAGTCGCGGTTTTGGTGTTTGAATGGCAGCCGCC
GCGATCAGGACGTAACGAGC

Downstream 100 bases:

>100_bases
CAGATTGCTGTCGGCGCGAAGCCGACAAAGAAATGCCGTTTCATTGACTTGCGATGGTTTGTTGACGAGCTTCGTACCTC
CTCCGCAGACGACGCGGATG

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MSELAQSVSLPSANPSEARARDLDAALRAASPPEIIAAALREVGRDRLALVSSFGTESAALLKVMADVDPAIPVVFLDTG
WLFAETLAYRDTLIDQLGLTDVRSIKPLDADLDRDDPARDLWFSDPDQCCFIRKVEPLRRALAPFEAWLNGRKRFQGGAR
AEIPVVEADGVRMKFNPFANVSREDINAIYALGKLPPHPLLASGFLSVGCMPCTSRAESDQDTRSGRWQGRGKTECGIHT
MKTS

Sequences:

>Translated_244_residues
MSELAQSVSLPSANPSEARARDLDAALRAASPPEIIAAALREVGRDRLALVSSFGTESAALLKVMADVDPAIPVVFLDTG
WLFAETLAYRDTLIDQLGLTDVRSIKPLDADLDRDDPARDLWFSDPDQCCFIRKVEPLRRALAPFEAWLNGRKRFQGGAR
AEIPVVEADGVRMKFNPFANVSREDINAIYALGKLPPHPLLASGFLSVGCMPCTSRAESDQDTRSGRWQGRGKTECGIHT
MKTS
>Mature_243_residues
SELAQSVSLPSANPSEARARDLDAALRAASPPEIIAAALREVGRDRLALVSSFGTESAALLKVMADVDPAIPVVFLDTGW
LFAETLAYRDTLIDQLGLTDVRSIKPLDADLDRDDPARDLWFSDPDQCCFIRKVEPLRRALAPFEAWLNGRKRFQGGARA
EIPVVEADGVRMKFNPFANVSREDINAIYALGKLPPHPLLASGFLSVGCMPCTSRAESDQDTRSGRWQGRGKTECGIHTM
KTS

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=196, Percent_Identity=32.6530612244898, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325425, Length=226, Percent_Identity=27.8761061946903, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 26654; Mature: 26522

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELAQSVSLPSANPSEARARDLDAALRAASPPEIIAAALREVGRDRLALVSSFGTESAA
CCHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCCHHH
LLKVMADVDPAIPVVFLDTGWLFAETLAYRDTLIDQLGLTDVRSIKPLDADLDRDDPARD
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCC
LWFSDPDQCCFIRKVEPLRRALAPFEAWLNGRKRFQGGARAEIPVVEADGVRMKFNPFAN
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCC
VSREDINAIYALGKLPPHPLLASGFLSVGCMPCTSRAESDQDTRSGRWQGRGKTECGIHT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEE
MKTS
CCCC
>Mature Secondary Structure 
SELAQSVSLPSANPSEARARDLDAALRAASPPEIIAAALREVGRDRLALVSSFGTESAA
CHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHCCCCHHH
LLKVMADVDPAIPVVFLDTGWLFAETLAYRDTLIDQLGLTDVRSIKPLDADLDRDDPARD
HHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCC
LWFSDPDQCCFIRKVEPLRRALAPFEAWLNGRKRFQGGARAEIPVVEADGVRMKFNPFAN
CCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCC
VSREDINAIYALGKLPPHPLLASGFLSVGCMPCTSRAESDQDTRSGRWQGRGKTECGIHT
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCEE
MKTS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]