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The map label for this gene is puuB [H]

Identifier: 86748162

GI number: 86748162

Start: 1191017

End: 1192318

Strand: Direct

Name: puuB [H]

Synonym: RPB_1037

Alternate gene names: 86748162

Gene position: 1191017-1192318 (Clockwise)

Preceding gene: 86748159

Following gene: 86748169

Centisome position: 22.34

GC content: 69.97

Gene sequence:

>1302_bases
ATGACTGCCGATGTGCGCTGGCCGGATTCGCTGTGGGCTGCGGTGACGCCGCCGGGGCCGGAGCTGCCCGAGCTGCGCGG
CACGGAGACGGCCGACGTCGTGGTGATCGGTGCCGGCTTCAGCGGGCTGTCGACGGCGCTGCATCTGCGCGAAGCGGGCG
TCGACGTCGCGGTGGTCGAGGCCGCCGAGCCGGGCTGGGGCGCGTCCGGTCGCAACAACGGCCAGGTGATCCCGACGCTG
TCGCGGCCCGATCCGGAGGACATCGTCGCCAAACACGGCGCCGCCGGCGAGCGGCTGGTGGGCCTCATTCGCGACAGCGC
CTCGATCCTGTTCGATGTGGTGAAGCGCCACGGCATCGCCGCCGAGCAGGAGCAGAATGGCTGGGTGCAGCCGGTGCATT
CGCCGGGCCGGATCAAGATCGCCGAGCGCCGCGTCCGGCAATGGTCGAAATTCGGCGCGCCGGTCGAATTGCTGTCGCGC
GACCAGATCCGCGCCATGCTCGGCTCCGACGCCTGGTTCGGCGGGTTCTGGAACAGGACCGGCGGCCACATCAATCCGCT
GGCGCTGGCGCGCGGGCTGGCACGCACGGTGCTGGCGCAGGGCGGCCGCATCTTTGCGCGCTCGCCGGCCGTGAGCATCG
AGCGGCAGGGCGACCGTTGGATCGTCAAGACCGCGCAGGGCGAGCTCAGCGGCCGTGCGCTGGTGGTCGCCAGCAACGCC
TACACCGGCGAGTTCGCCAAGACGCTGGCGCCGGCGATCGCAAGTGAAGTGATGCCGGTGCGGTCGTGGCAGATGGCGAC
GCAGCCGCTCGGCGATAATGTGCGCAAGAGCATGATCCCCGGCCGTCAGGCGATGTCGGATACGCATGGCGAGCTGATCT
TCGGCCGCTTCGACGCCCGCAACCGCTTCGTCACCGGCGGCGCGATCATCCGGCCGTTCAATCAGGCCGCGACCTTGCGG
GCGCGTGTCGGCAAGCGGTTGCAGAGCCTGTGGCCGCAGATCGGCGAGGTGCAGTTCGACTACGTCTGGAACGGCTTCAT
CGGCATGACCACGGACTATCTGCCGCACATCCACCGCCTCGGCCCCAATGCCTATGGCTGGACCGGCTGCAACGGCCGCG
GCGTCGCGCTGTCGATCGCGCTCGGCGACGAATTGTCGAAAGCGGTGCGCGGCGTGCCGGACGGCGAACTGGCGCTGCCC
TTCACCGAGCCGACGCCGATCCCCGCCAACGAACTGCTCCGCCCCTTCGCGCCGCTGATGATGGTGCTGTACCGGCACCG
CGACAGCAAGGAGATCGCGTAG

Upstream 100 bases:

>100_bases
CCAAAATTCTTGCATATAAGGCTAGCGACATCGCCGCGGCGCCGCAACCCGACGCATGTCGGAGGTGGGGGCGATTGAAA
TCGATATGGAAGGATTGAGG

Downstream 100 bases:

>100_bases
TTCGCCTGCCTGCGCGATTCCGCTCCAACCTTTCAACCGTCATGCCCGGGCTCGTCCCGGGCATCCACGTTCTCGAGCCG
TTCAGCATTGGAAGACGTGG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MTADVRWPDSLWAAVTPPGPELPELRGTETADVVVIGAGFSGLSTALHLREAGVDVAVVEAAEPGWGASGRNNGQVIPTL
SRPDPEDIVAKHGAAGERLVGLIRDSASILFDVVKRHGIAAEQEQNGWVQPVHSPGRIKIAERRVRQWSKFGAPVELLSR
DQIRAMLGSDAWFGGFWNRTGGHINPLALARGLARTVLAQGGRIFARSPAVSIERQGDRWIVKTAQGELSGRALVVASNA
YTGEFAKTLAPAIASEVMPVRSWQMATQPLGDNVRKSMIPGRQAMSDTHGELIFGRFDARNRFVTGGAIIRPFNQAATLR
ARVGKRLQSLWPQIGEVQFDYVWNGFIGMTTDYLPHIHRLGPNAYGWTGCNGRGVALSIALGDELSKAVRGVPDGELALP
FTEPTPIPANELLRPFAPLMMVLYRHRDSKEIA

Sequences:

>Translated_433_residues
MTADVRWPDSLWAAVTPPGPELPELRGTETADVVVIGAGFSGLSTALHLREAGVDVAVVEAAEPGWGASGRNNGQVIPTL
SRPDPEDIVAKHGAAGERLVGLIRDSASILFDVVKRHGIAAEQEQNGWVQPVHSPGRIKIAERRVRQWSKFGAPVELLSR
DQIRAMLGSDAWFGGFWNRTGGHINPLALARGLARTVLAQGGRIFARSPAVSIERQGDRWIVKTAQGELSGRALVVASNA
YTGEFAKTLAPAIASEVMPVRSWQMATQPLGDNVRKSMIPGRQAMSDTHGELIFGRFDARNRFVTGGAIIRPFNQAATLR
ARVGKRLQSLWPQIGEVQFDYVWNGFIGMTTDYLPHIHRLGPNAYGWTGCNGRGVALSIALGDELSKAVRGVPDGELALP
FTEPTPIPANELLRPFAPLMMVLYRHRDSKEIA
>Mature_432_residues
TADVRWPDSLWAAVTPPGPELPELRGTETADVVVIGAGFSGLSTALHLREAGVDVAVVEAAEPGWGASGRNNGQVIPTLS
RPDPEDIVAKHGAAGERLVGLIRDSASILFDVVKRHGIAAEQEQNGWVQPVHSPGRIKIAERRVRQWSKFGAPVELLSRD
QIRAMLGSDAWFGGFWNRTGGHINPLALARGLARTVLAQGGRIFARSPAVSIERQGDRWIVKTAQGELSGRALVVASNAY
TGEFAKTLAPAIASEVMPVRSWQMATQPLGDNVRKSMIPGRQAMSDTHGELIFGRFDARNRFVTGGAIIRPFNQAATLRA
RVGKRLQSLWPQIGEVQFDYVWNGFIGMTTDYLPHIHRLGPNAYGWTGCNGRGVALSIALGDELSKAVRGVPDGELALPF
TEPTPIPANELLRPFAPLMMVLYRHRDSKEIA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=422, Percent_Identity=31.042654028436, Blast_Score=207, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46938; Mature: 46807

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADVRWPDSLWAAVTPPGPELPELRGTETADVVVIGAGFSGLSTALHLREAGVDVAVVE
CCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEE
AAEPGWGASGRNNGQVIPTLSRPDPEDIVAKHGAAGERLVGLIRDSASILFDVVKRHGIA
ECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
AEQEQNGWVQPVHSPGRIKIAERRVRQWSKFGAPVELLSRDQIRAMLGSDAWFGGFWNRT
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GGHINPLALARGLARTVLAQGGRIFARSPAVSIERQGDRWIVKTAQGELSGRALVVASNA
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEEECCCCCCCCEEEEECCC
YTGEFAKTLAPAIASEVMPVRSWQMATQPLGDNVRKSMIPGRQAMSDTHGELIFGRFDAR
CCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHCCCCCCEEEEEECCC
NRFVTGGAIIRPFNQAATLRARVGKRLQSLWPQIGEVQFDYVWNGFIGMTTDYLPHIHRL
CEEEECCEEECCCHHHHHHHHHHHHHHHHHCHHHCCEEEEEEECCCHHCCHHHHHHHHHC
GPNAYGWTGCNGRGVALSIALGDELSKAVRGVPDGELALPFTEPTPIPANELLRPFAPLM
CCCCCCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
MVLYRHRDSKEIA
HHHHHCCCCCCCC
>Mature Secondary Structure 
TADVRWPDSLWAAVTPPGPELPELRGTETADVVVIGAGFSGLSTALHLREAGVDVAVVE
CCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEE
AAEPGWGASGRNNGQVIPTLSRPDPEDIVAKHGAAGERLVGLIRDSASILFDVVKRHGIA
ECCCCCCCCCCCCCCEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
AEQEQNGWVQPVHSPGRIKIAERRVRQWSKFGAPVELLSRDQIRAMLGSDAWFGGFWNRT
CCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
GGHINPLALARGLARTVLAQGGRIFARSPAVSIERQGDRWIVKTAQGELSGRALVVASNA
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCEEEEEECCCCCCCCEEEEECCC
YTGEFAKTLAPAIASEVMPVRSWQMATQPLGDNVRKSMIPGRQAMSDTHGELIFGRFDAR
CCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHCCCCCCEEEEEECCC
NRFVTGGAIIRPFNQAATLRARVGKRLQSLWPQIGEVQFDYVWNGFIGMTTDYLPHIHRL
CEEEECCEEECCCHHHHHHHHHHHHHHHHHCHHHCCEEEEEEECCCHHCCHHHHHHHHHC
GPNAYGWTGCNGRGVALSIALGDELSKAVRGVPDGELALPFTEPTPIPANELLRPFAPLM
CCCCCCCCCCCCCCEEEEEEECHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
MVLYRHRDSKEIA
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]