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The map label for this gene is otsB [H]

Identifier: 86748042

GI number: 86748042

Start: 1057231

End: 1058037

Strand: Direct

Name: otsB [H]

Synonym: RPB_0916

Alternate gene names: 86748042

Gene position: 1057231-1058037 (Clockwise)

Preceding gene: 86748041

Following gene: 86748043

Centisome position: 19.83

GC content: 66.67

Gene sequence:

>807_bases
ATGACTTCGAATCTCGCCGACGACGCCTCGCGGCCGACTACCAATTCCGTACCGGTGCCGAGCGCGCTGGTGCCGCATCT
CGATCAGTGCGCGCTGCTGCTCGATATCGACGGCACGCTGCTCGACCTCGCGCCGACGCCGCGCGAAGTCTGGGTGCCGC
CGGAACTCGAACAGACGCTGCGCGCGCTGCACGAGCGCACCTCCGGCGCGCTGGCGCTGGTCAGCGGCCGTTCGATCAAC
GATATCGATCTGATCTTCGCGCCGCTGAAACTGCCCGCCGTCGGCGGCCACGGCGCCGAGATGCGGCTGTTGTCCAGCGC
CGAACAGGTCGCGACCCGCGCGCCGCCGCTGGACCCCGACTTGAAAAATCGTCTCGCCGCGATCGCGCGGATCAGTCCCG
GCATTCTGCTCGAAGACAAAGGCTACTCGCTGGCGCTGCACTACCGTCTGGCGCCGCACACCGAGAAAGCGATCTACGAA
TCCGTCGCCGCGATCCGCGCCGAGAATCCGCACGCGCCGCTCGAAGTGCTGCCGGGCAAGTCGGTGTGCGAGATCAAGCA
TGCCGGCGTCACCAAGGCGACCGGCGTGCTCGAACTGATGAATCACGAACCGTTCAAAGGTCGGCGCCCGGTGTTCCTGG
GCGACGACGTCACCGACGAGACGGTGTTCGCGATCATGCCGGAACTACGCGGTCTGGCATTTTCGGTGGGCCGCCACAGC
GACATCGTCGCGGGACATTTCGACGAGCCGCGCGATGTGCGGGCCTGGCTGGCGCGGTTGCTCGATCCACAGTTAGCCCC
AGGTTAG

Upstream 100 bases:

>100_bases
TGATCGTCGCCGCAATGGTCGGTTGGCGTTATGGTGTGGCGGCCGAACGCAGGTCGCTGGAACAAGTGGCGCGGCCGCTG
GCCGCCATAGAGGAAAACCG

Downstream 100 bases:

>100_bases
CCCCAAGTTAGCCGCAACACGTCGGGGTTCCGCGTCGATCCGTGTCGTGCCGCAAAGTCGTTCAATTTGCGCCATGGAAC
GAACTTGATGAACGGTTGTT

Product: trehalose-6-phosphatase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTSNLADDASRPTTNSVPVPSALVPHLDQCALLLDIDGTLLDLAPTPREVWVPPELEQTLRALHERTSGALALVSGRSIN
DIDLIFAPLKLPAVGGHGAEMRLLSSAEQVATRAPPLDPDLKNRLAAIARISPGILLEDKGYSLALHYRLAPHTEKAIYE
SVAAIRAENPHAPLEVLPGKSVCEIKHAGVTKATGVLELMNHEPFKGRRPVFLGDDVTDETVFAIMPELRGLAFSVGRHS
DIVAGHFDEPRDVRAWLARLLDPQLAPG

Sequences:

>Translated_268_residues
MTSNLADDASRPTTNSVPVPSALVPHLDQCALLLDIDGTLLDLAPTPREVWVPPELEQTLRALHERTSGALALVSGRSIN
DIDLIFAPLKLPAVGGHGAEMRLLSSAEQVATRAPPLDPDLKNRLAAIARISPGILLEDKGYSLALHYRLAPHTEKAIYE
SVAAIRAENPHAPLEVLPGKSVCEIKHAGVTKATGVLELMNHEPFKGRRPVFLGDDVTDETVFAIMPELRGLAFSVGRHS
DIVAGHFDEPRDVRAWLARLLDPQLAPG
>Mature_267_residues
TSNLADDASRPTTNSVPVPSALVPHLDQCALLLDIDGTLLDLAPTPREVWVPPELEQTLRALHERTSGALALVSGRSIND
IDLIFAPLKLPAVGGHGAEMRLLSSAEQVATRAPPLDPDLKNRLAAIARISPGILLEDKGYSLALHYRLAPHTEKAIYES
VAAIRAENPHAPLEVLPGKSVCEIKHAGVTKATGVLELMNHEPFKGRRPVFLGDDVTDETVFAIMPELRGLAFSVGRHSD
IVAGHFDEPRDVRAWLARLLDPQLAPG

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=229, Percent_Identity=36.6812227074236, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 28904; Mature: 28772

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSNLADDASRPTTNSVPVPSALVPHLDQCALLLDIDGTLLDLAPTPREVWVPPELEQTL
CCCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCEECCCHHHHHH
RALHERTSGALALVSGRSINDIDLIFAPLKLPAVGGHGAEMRLLSSAEQVATRAPPLDPD
HHHHHHCCCCEEEEECCCCCCEEEEEECEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHH
LKNRLAAIARISPGILLEDKGYSLALHYRLAPHTEKAIYESVAAIRAENPHAPLEVLPGK
HHHHHHHHHHCCCCEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEECCCC
SVCEIKHAGVTKATGVLELMNHEPFKGRRPVFLGDDVTDETVFAIMPELRGLAFSVGRHS
CEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCC
DIVAGHFDEPRDVRAWLARLLDPQLAPG
CEEECCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSNLADDASRPTTNSVPVPSALVPHLDQCALLLDIDGTLLDLAPTPREVWVPPELEQTL
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEEEEECCCCEEECCCCCCCEECCCHHHHHH
RALHERTSGALALVSGRSINDIDLIFAPLKLPAVGGHGAEMRLLSSAEQVATRAPPLDPD
HHHHHHCCCCEEEEECCCCCCEEEEEECEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHH
LKNRLAAIARISPGILLEDKGYSLALHYRLAPHTEKAIYESVAAIRAENPHAPLEVLPGK
HHHHHHHHHHCCCCEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEECCCC
SVCEIKHAGVTKATGVLELMNHEPFKGRRPVFLGDDVTDETVFAIMPELRGLAFSVGRHS
CEEEECCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHEEECCCCC
DIVAGHFDEPRDVRAWLARLLDPQLAPG
CEEECCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]