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The map label for this gene is otsA [H]

Identifier: 86748043

GI number: 86748043

Start: 1058173

End: 1059657

Strand: Direct

Name: otsA [H]

Synonym: RPB_0917

Alternate gene names: 86748043

Gene position: 1058173-1059657 (Clockwise)

Preceding gene: 86748042

Following gene: 86748047

Centisome position: 19.85

GC content: 66.2

Gene sequence:

>1485_bases
GTGAACCTAGTCGTCGTTTCAAACCGGGTGGCGAGGGCGTCTTCGAACGAGCCGATGACCGGTGGTCTGGCCGCCGCCTT
GCTGCCCGTGGTCGAAAAGTCCGGCGCCATCTGGGTCGGTTCCAGCGGCCGGGTGCGCGATGGCGCGCAACGGGAACCCT
TTGCGGAGATTGAACAACTCGGCGCCGGCGCGCTGGCGATGCTGGATCTGCCGGCGGCGCATTACGGCGGTTATTACGAG
GGCTTCGCCAATTCCGCGCTGTGGCCGGCGCTGCATTCCCGCGCCGATCTGATCCGCGTCTCGCAGGACGACTACCGTTC
CTATCGCGAGGTCAATAGCTTCATGGCGCGCGCCCTGCTGCGCTTCCGCAAGCCCGACACTGCGTTCTGGATACAGGATT
ACCATTTTCTCGCCCTCGGCGCGGAGTTGCGCGCCCTCGGCGTGACCCAGCCGATCGGCTTCTTCCTGCACACGCCGTGG
GCGTCCCCGGCGACGATGGGCTGCGTGCCGCACAATCGCGAGCTGGTCGAGGCGATGCTCGCCTATGATCTGATCGGATT
CCAGACCGAAGAGGACCGGAGCAATTTTCTGGCCTATGGCAAAGCCGAACTCGGCTTTGCCATCGCCGACGGCGTCGTCA
CGACGCCGTACGGCACATCGCGTTGTGAAGTGTTCCCGATCGGCATCGATGCCGATCTGTTTGCGCAGCAGGCGCAGAAG
GCGACCGCGCATCCCGATGTGTCGCGGCTGCGCAAGAGCCTCAACGGCGAGAAGCTCGTGATCGGCGTCGACCGCCTGGA
TTATTCGAAGGGTTTGATCAACCGCGTCAATGCCTTCGACCGAATGCTGACCATGCGACCGTCGCTGCAACGCACCGTGT
CATTGCTGCAGATCGCGACCCCGTCGCGCGGCACGATCGAGGCCTATGGCAATCTGCAGGGCGAACTCGCCAAGCTCGTC
AGCGACGTCAACGGCCGGCTCGGCGAGGCCGACTGGACCCCGATCCGCTATCTCAACAAGGGCTTCCGTCAGGGCGTGCT
GGCGGGGCTGTACCGCACCGCGCAGGTCGGTCTTGTCACGCCGCTGCAGGACGGCATGAATCTGGTGGCGAAGGAATACG
TCGCCGCGCAGAATCCGATCGACCCCGGCGTGCTGGTGCTGTCGAAATTCGCGGGCGCCGCCAACGAGCTCGATACCGCG
CTGCTGGTCAATCCGCACGACGTCGAAGGCATGGCCCGCGCCATCGCCACCGCATTGTCGATGCCCCTGACCGAGCGCCG
GCTGCGCTGGGAAGCGATGATGGCCAAGCTGCGCCGCGGCAGCGTCCAGTCCTGGTTCGCGGATTTCGTGGCGTCGCTGG
AAGACGCCCATACCGCCAACAGCGACGCCGCCGGCGCCCTCGCCAGCCAGCCGCCGGCGCTGAAAATGGCCGGCGGCTGG
TCGCGGGGCGGGCCTCTGGCCTTCGGCGGCGCGCGGCTGCAATAG

Upstream 100 bases:

>100_bases
CGATCCGTGTCGTGCCGCAAAGTCGTTCAATTTGCGCCATGGAACGAACTTGATGAACGGTTGTTAATAAGGGCAAATCA
ACAGGGGCGCGGAGGGACAC

Downstream 100 bases:

>100_bases
GAGGCTGCCGACGCTGCGCCGGGGACGGGCGCTGCTGCCCGCCCGGATTCGCGCAACGATTTTGTCGCCGGCGGCCCTCC
GGCCTTGGGCTTTCCGATGG

Product: Alpha,alpha-trehalose-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNLVVVSNRVARASSNEPMTGGLAAALLPVVEKSGAIWVGSSGRVRDGAQREPFAEIEQLGAGALAMLDLPAAHYGGYYE
GFANSALWPALHSRADLIRVSQDDYRSYREVNSFMARALLRFRKPDTAFWIQDYHFLALGAELRALGVTQPIGFFLHTPW
ASPATMGCVPHNRELVEAMLAYDLIGFQTEEDRSNFLAYGKAELGFAIADGVVTTPYGTSRCEVFPIGIDADLFAQQAQK
ATAHPDVSRLRKSLNGEKLVIGVDRLDYSKGLINRVNAFDRMLTMRPSLQRTVSLLQIATPSRGTIEAYGNLQGELAKLV
SDVNGRLGEADWTPIRYLNKGFRQGVLAGLYRTAQVGLVTPLQDGMNLVAKEYVAAQNPIDPGVLVLSKFAGAANELDTA
LLVNPHDVEGMARAIATALSMPLTERRLRWEAMMAKLRRGSVQSWFADFVASLEDAHTANSDAAGALASQPPALKMAGGW
SRGGPLAFGGARLQ

Sequences:

>Translated_494_residues
MNLVVVSNRVARASSNEPMTGGLAAALLPVVEKSGAIWVGSSGRVRDGAQREPFAEIEQLGAGALAMLDLPAAHYGGYYE
GFANSALWPALHSRADLIRVSQDDYRSYREVNSFMARALLRFRKPDTAFWIQDYHFLALGAELRALGVTQPIGFFLHTPW
ASPATMGCVPHNRELVEAMLAYDLIGFQTEEDRSNFLAYGKAELGFAIADGVVTTPYGTSRCEVFPIGIDADLFAQQAQK
ATAHPDVSRLRKSLNGEKLVIGVDRLDYSKGLINRVNAFDRMLTMRPSLQRTVSLLQIATPSRGTIEAYGNLQGELAKLV
SDVNGRLGEADWTPIRYLNKGFRQGVLAGLYRTAQVGLVTPLQDGMNLVAKEYVAAQNPIDPGVLVLSKFAGAANELDTA
LLVNPHDVEGMARAIATALSMPLTERRLRWEAMMAKLRRGSVQSWFADFVASLEDAHTANSDAAGALASQPPALKMAGGW
SRGGPLAFGGARLQ
>Mature_494_residues
MNLVVVSNRVARASSNEPMTGGLAAALLPVVEKSGAIWVGSSGRVRDGAQREPFAEIEQLGAGALAMLDLPAAHYGGYYE
GFANSALWPALHSRADLIRVSQDDYRSYREVNSFMARALLRFRKPDTAFWIQDYHFLALGAELRALGVTQPIGFFLHTPW
ASPATMGCVPHNRELVEAMLAYDLIGFQTEEDRSNFLAYGKAELGFAIADGVVTTPYGTSRCEVFPIGIDADLFAQQAQK
ATAHPDVSRLRKSLNGEKLVIGVDRLDYSKGLINRVNAFDRMLTMRPSLQRTVSLLQIATPSRGTIEAYGNLQGELAKLV
SDVNGRLGEADWTPIRYLNKGFRQGVLAGLYRTAQVGLVTPLQDGMNLVAKEYVAAQNPIDPGVLVLSKFAGAANELDTA
LLVNPHDVEGMARAIATALSMPLTERRLRWEAMMAKLRRGSVQSWFADFVASLEDAHTANSDAAGALASQPPALKMAGGW
SRGGPLAFGGARLQ

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=453, Percent_Identity=39.9558498896247, Blast_Score=327, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI71987012, Length=356, Percent_Identity=29.2134831460674, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI115533560, Length=350, Percent_Identity=28.8571428571429, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI115533558, Length=350, Percent_Identity=28.8571428571429, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6319602, Length=474, Percent_Identity=30.5907172995781, Blast_Score=239, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6320279, Length=419, Percent_Identity=25.2983293556086, Blast_Score=156, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6323537, Length=392, Percent_Identity=24.4897959183673, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323917, Length=448, Percent_Identity=23.6607142857143, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19920676, Length=489, Percent_Identity=35.1738241308793, Blast_Score=260, Evalue=2e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 53494; Mature: 53494

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVVVSNRVARASSNEPMTGGLAAALLPVVEKSGAIWVGSSGRVRDGAQREPFAEIEQL
CEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHC
GAGALAMLDLPAAHYGGYYEGFANSALWPALHSRADLIRVSQDDYRSYREVNSFMARALL
CCCEEEEEECCHHHHCCHHHHHCCCCHHHHHHCCHHEEEECHHHHHHHHHHHHHHHHHHH
RFRKPDTAFWIQDYHFLALGAELRALGVTQPIGFFLHTPWASPATMGCVPHNRELVEAML
HHCCCCCEEEEECCEEEEECCHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
AYDLIGFQTEEDRSNFLAYGKAELGFAIADGVVTTPYGTSRCEVFPIGIDADLFAQQAQK
HHHHHCCCCCCCCCCEEEECCHHCCEEEECCEEECCCCCCCCEEEEECCCHHHHHHHHHH
ATAHPDVSRLRKSLNGEKLVIGVDRLDYSKGLINRVNAFDRMLTMRPSLQRTVSLLQIAT
CCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
PSRGTIEAYGNLQGELAKLVSDVNGRLGEADWTPIRYLNKGFRQGVLAGLYRTAQVGLVT
CCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEC
PLQDGMNLVAKEYVAAQNPIDPGVLVLSKFAGAANELDTALLVNPHDVEGMARAIATALS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
MPLTERRLRWEAMMAKLRRGSVQSWFADFVASLEDAHTANSDAAGALASQPPALKMAGGW
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCC
SRGGPLAFGGARLQ
CCCCCEECCCCCCC
>Mature Secondary Structure
MNLVVVSNRVARASSNEPMTGGLAAALLPVVEKSGAIWVGSSGRVRDGAQREPFAEIEQL
CEEEEECCCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHC
GAGALAMLDLPAAHYGGYYEGFANSALWPALHSRADLIRVSQDDYRSYREVNSFMARALL
CCCEEEEEECCHHHHCCHHHHHCCCCHHHHHHCCHHEEEECHHHHHHHHHHHHHHHHHHH
RFRKPDTAFWIQDYHFLALGAELRALGVTQPIGFFLHTPWASPATMGCVPHNRELVEAML
HHCCCCCEEEEECCEEEEECCHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
AYDLIGFQTEEDRSNFLAYGKAELGFAIADGVVTTPYGTSRCEVFPIGIDADLFAQQAQK
HHHHHCCCCCCCCCCEEEECCHHCCEEEECCEEECCCCCCCCEEEEECCCHHHHHHHHHH
ATAHPDVSRLRKSLNGEKLVIGVDRLDYSKGLINRVNAFDRMLTMRPSLQRTVSLLQIAT
CCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
PSRGTIEAYGNLQGELAKLVSDVNGRLGEADWTPIRYLNKGFRQGVLAGLYRTAQVGLVT
CCCCCEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEC
PLQDGMNLVAKEYVAAQNPIDPGVLVLSKFAGAANELDTALLVNPHDVEGMARAIATALS
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
MPLTERRLRWEAMMAKLRRGSVQSWFADFVASLEDAHTANSDAAGALASQPPALKMAGGW
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCC
SRGGPLAFGGARLQ
CCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA