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The map label for this gene is yafC [H]

Identifier: 86747948

GI number: 86747948

Start: 937700

End: 938614

Strand: Reverse

Name: yafC [H]

Synonym: RPB_0822

Alternate gene names: 86747948

Gene position: 938614-937700 (Counterclockwise)

Preceding gene: 86747951

Following gene: 86747942

Centisome position: 17.6

GC content: 66.67

Gene sequence:

>915_bases
ATGGATAAATCAGCCATCACCATCGAACGGATCCGGACCTTCGTGCGCATCGCCGAGCGCGGAAGCCTGTCGGCGGTCGC
CCGCGATTTGAATGTCGGCCAGTCCACCGTGACGCGGCAGTTGCAGGAACTCGAAGCGGCGATCGGCGCACCTTTGCTGA
GCCGCACCACCCGCAGAGTGACGCTGACCGAGGAAGGCAGCCGCTATTATGCGCGCTGCGTCCAGATCCTGCGGCTGCTC
GAGCAGGCGGGCGACGAAGCGCTCGGCGCGTCCGGCGCGCAGGCCGGCACCATCCGGATCTCCTGCACGGCATCGTTCGG
CATTCTGCACGTCTGCCGGCTGATCTTCGCGTTCCAAGATCGCTATCCGGAGATCGGGGTCGATCTCGGCCTGACCGATC
ACCGGATCGATCTGGTTCGCGAAGGCGTGGACCTGGCGCTTCGCCTGGCGCCGCTCACCGAGAGTTCGATGAAACTCCGC
ACGCTCGGCCACAGCCAGCGCCTGCTGGTGGCCTCGCCGGACTATCTGTCGGCTCACGGCCGACCGACGTCGCCGCATGA
TCTGTCCGGTCATCAGGGCGTCCGCATGATGAACGTCGCGGGCAGCGACATGCTGGTGCTGCAGGGGCCGGGTGAGGAGA
CTCATCGGGTGCCGTTCGGCGGCAAGCTCAGGGTCGACCACGGGCTCGCGGCGCGGGAGGCCATGATCGCCGGGCGCGGC
ATCGCGGCGACGCATCGCTGGCTGGTCGATGATCTGCTGGCCGCAGGGCGGCTCGAGGTCATTCTGCCGGAATATCGACT
GGCCGCGGTGCCGCTCAATTTGCTGATCGTGCCGGAGCGCGCCGGCATCGCACGTGTCCGGCTACTGATCGACTTCCTGG
TGGAGCAGATCAGAACCGTGCCGGGAATCGCGTAA

Upstream 100 bases:

>100_bases
TTTCATGGCCGTGGCTCCTCGATGATTGATCGGAGCCTCCGAGTAGACCGGCCGCGACTATTCCGGTAGGCGTTGAGTTT
CGATATGATTGTGCCGAAAA

Downstream 100 bases:

>100_bases
GGCGCTCAGCGCGTCGGGACCGGCTTGTCGCCGCGATAATCGTAGAAGCCACGCTGGGTCTTGCGGCCGAGCCAGCCGGC
CTCGACGTATTTCACCAGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MDKSAITIERIRTFVRIAERGSLSAVARDLNVGQSTVTRQLQELEAAIGAPLLSRTTRRVTLTEEGSRYYARCVQILRLL
EQAGDEALGASGAQAGTIRISCTASFGILHVCRLIFAFQDRYPEIGVDLGLTDHRIDLVREGVDLALRLAPLTESSMKLR
TLGHSQRLLVASPDYLSAHGRPTSPHDLSGHQGVRMMNVAGSDMLVLQGPGEETHRVPFGGKLRVDHGLAAREAMIAGRG
IAATHRWLVDDLLAAGRLEVILPEYRLAAVPLNLLIVPERAGIARVRLLIDFLVEQIRTVPGIA

Sequences:

>Translated_304_residues
MDKSAITIERIRTFVRIAERGSLSAVARDLNVGQSTVTRQLQELEAAIGAPLLSRTTRRVTLTEEGSRYYARCVQILRLL
EQAGDEALGASGAQAGTIRISCTASFGILHVCRLIFAFQDRYPEIGVDLGLTDHRIDLVREGVDLALRLAPLTESSMKLR
TLGHSQRLLVASPDYLSAHGRPTSPHDLSGHQGVRMMNVAGSDMLVLQGPGEETHRVPFGGKLRVDHGLAAREAMIAGRG
IAATHRWLVDDLLAAGRLEVILPEYRLAAVPLNLLIVPERAGIARVRLLIDFLVEQIRTVPGIA
>Mature_304_residues
MDKSAITIERIRTFVRIAERGSLSAVARDLNVGQSTVTRQLQELEAAIGAPLLSRTTRRVTLTEEGSRYYARCVQILRLL
EQAGDEALGASGAQAGTIRISCTASFGILHVCRLIFAFQDRYPEIGVDLGLTDHRIDLVREGVDLALRLAPLTESSMKLR
TLGHSQRLLVASPDYLSAHGRPTSPHDLSGHQGVRMMNVAGSDMLVLQGPGEETHRVPFGGKLRVDHGLAAREAMIAGRG
IAATHRWLVDDLLAAGRLEVILPEYRLAAVPLNLLIVPERAGIARVRLLIDFLVEQIRTVPGIA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=32.996632996633, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI87081978, Length=286, Percent_Identity=32.8671328671329, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789440, Length=283, Percent_Identity=30.0353356890459, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789639, Length=292, Percent_Identity=27.0547945205479, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=33.1081081081081, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1787128, Length=254, Percent_Identity=28.740157480315, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693105, Length=238, Percent_Identity=23.5294117647059, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789173, Length=291, Percent_Identity=25.085910652921, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=177, Percent_Identity=29.9435028248588, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787601, Length=139, Percent_Identity=31.6546762589928, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1787879, Length=121, Percent_Identity=34.7107438016529, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787589, Length=190, Percent_Identity=28.4210526315789, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=174, Percent_Identity=27.0114942528736, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33122; Mature: 33122

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSAITIERIRTFVRIAERGSLSAVARDLNVGQSTVTRQLQELEAAIGAPLLSRTTRRV
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCCEEE
TLTEEGSRYYARCVQILRLLEQAGDEALGASGAQAGTIRISCTASFGILHVCRLIFAFQD
EEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHH
RYPEIGVDLGLTDHRIDLVREGVDLALRLAPLTESSMKLRTLGHSQRLLVASPDYLSAHG
CCCCCCEECCCCHHHHHHHHCCCCEEEEEECCCCCCHHHEECCCCCEEEEECCCHHCCCC
RPTSPHDLSGHQGVRMMNVAGSDMLVLQGPGEETHRVPFGGKLRVDHGLAAREAMIAGRG
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCC
IAATHRWLVDDLLAAGRLEVILPEYRLAAVPLNLLIVPERAGIARVRLLIDFLVEQIRTV
HHHHHHHHHHHHHHCCCEEEEECCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHC
PGIA
CCCC
>Mature Secondary Structure
MDKSAITIERIRTFVRIAERGSLSAVARDLNVGQSTVTRQLQELEAAIGAPLLSRTTRRV
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCCEEE
TLTEEGSRYYARCVQILRLLEQAGDEALGASGAQAGTIRISCTASFGILHVCRLIFAFQD
EEECCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHH
RYPEIGVDLGLTDHRIDLVREGVDLALRLAPLTESSMKLRTLGHSQRLLVASPDYLSAHG
CCCCCCEECCCCHHHHHHHHCCCCEEEEEECCCCCCHHHEECCCCCEEEEECCCHHCCCC
RPTSPHDLSGHQGVRMMNVAGSDMLVLQGPGEETHRVPFGGKLRVDHGLAAREAMIAGRG
CCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHCCC
IAATHRWLVDDLLAAGRLEVILPEYRLAAVPLNLLIVPERAGIARVRLLIDFLVEQIRTV
HHHHHHHHHHHHHHCCCEEEEECCCEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHC
PGIA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]