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The map label for this gene is ogt [H]

Identifier: 86747926

GI number: 86747926

Start: 897297

End: 897875

Strand: Reverse

Name: ogt [H]

Synonym: RPB_0800

Alternate gene names: 86747926

Gene position: 897875-897297 (Counterclockwise)

Preceding gene: 86747927

Following gene: 86747925

Centisome position: 16.84

GC content: 70.81

Gene sequence:

>579_bases
ATGGTGGAGGTTGCGTACACGATCTTCGACACGATGATGGGCCGATGCGGCATCGCATGGGGGCTCGACGGAGTGCTCGT
CGTCCAGCTCGCCGAAAGCCGCGAGATCGACACCCGCCGCCGGCTGCTGAAGCAATGTCCCGATGCCCGCGAGGCCCGGC
CGACGGTCGGAATCGACAGCGCGATCGAAGGCGTCGCGGCGATCTTGCGCGGCGCGCATTGGGATTTTTCCGACGTCCGG
CTCGACCTCGGCGGCCTGCCGGCGTTCGACTGCCGGGTCTACGACATCCTCCGCGACATCCCGCGTGGCGAGACCGCGAC
CTATGCGGAGATCGCCAAGCGGGCCGGCTCGCCGGGCGCATTGCATGCGGTCGGCCATGCGATCCGCAGCAATCCGTTCG
CCGTGATCGTGCCGTGCCATCGCGCGCTGCCGGTCGCCGGCGACACCAGCGGCAGCAGCGCCAATGGCGGCGCCATCACC
CGGCGGCGCCTGCTGGCGATCGAGGGCGCGCTGGCGACCCGCGGCCCGAGCCTGTTCGACGTGCTGCTCACCGCCACGCC
GCGCCAGGCGGATCGCTAG

Upstream 100 bases:

>100_bases
CGCGGCATGTCGGCCGGTCGACATGTCGCCCCAGCAACAGCGTTAACAATTTGTAAAGATTCCGGATGCGCGGTTCCACG
GCGCTGGTATAGTCCAACCC

Downstream 100 bases:

>100_bases
AGCTTGTCCGGTTCGATGAATCAGAACCGGAGCTCCGGATTCTCGATTTGACGCGTTTTCTTCGCGCGAACCGGTATCCA
CTTCGCTCGACAATGCTGTC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MVEVAYTIFDTMMGRCGIAWGLDGVLVVQLAESREIDTRRRLLKQCPDAREARPTVGIDSAIEGVAAILRGAHWDFSDVR
LDLGGLPAFDCRVYDILRDIPRGETATYAEIAKRAGSPGALHAVGHAIRSNPFAVIVPCHRALPVAGDTSGSSANGGAIT
RRRLLAIEGALATRGPSLFDVLLTATPRQADR

Sequences:

>Translated_192_residues
MVEVAYTIFDTMMGRCGIAWGLDGVLVVQLAESREIDTRRRLLKQCPDAREARPTVGIDSAIEGVAAILRGAHWDFSDVR
LDLGGLPAFDCRVYDILRDIPRGETATYAEIAKRAGSPGALHAVGHAIRSNPFAVIVPCHRALPVAGDTSGSSANGGAIT
RRRLLAIEGALATRGPSLFDVLLTATPRQADR
>Mature_192_residues
MVEVAYTIFDTMMGRCGIAWGLDGVLVVQLAESREIDTRRRLLKQCPDAREARPTVGIDSAIEGVAAILRGAHWDFSDVR
LDLGGLPAFDCRVYDILRDIPRGETATYAEIAKRAGSPGALHAVGHAIRSNPFAVIVPCHRALPVAGDTSGSSANGGAIT
RRRLLAIEGALATRGPSLFDVLLTATPRQADR

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=166, Percent_Identity=31.3253012048193, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1787596, Length=88, Percent_Identity=42.0454545454545, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=171, Percent_Identity=32.1637426900585, Blast_Score=71, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115533070, Length=86, Percent_Identity=44.1860465116279, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20530; Mature: 20530

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVAYTIFDTMMGRCGIAWGLDGVLVVQLAESREIDTRRRLLKQCPDAREARPTVGIDS
CEEEHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
AIEGVAAILRGAHWDFSDVRLDLGGLPAFDCRVYDILRDIPRGETATYAEIAKRAGSPGA
HHHHHHHHHHCCCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCH
LHAVGHAIRSNPFAVIVPCHRALPVAGDTSGSSANGGAITRRRLLAIEGALATRGPSLFD
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCHHHH
VLLTATPRQADR
HHHHCCCCCCCC
>Mature Secondary Structure
MVEVAYTIFDTMMGRCGIAWGLDGVLVVQLAESREIDTRRRLLKQCPDAREARPTVGIDS
CEEEHHHHHHHHHHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHH
AIEGVAAILRGAHWDFSDVRLDLGGLPAFDCRVYDILRDIPRGETATYAEIAKRAGSPGA
HHHHHHHHHHCCCCCHHHEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCH
LHAVGHAIRSNPFAVIVPCHRALPVAGDTSGSSANGGAITRRRLLAIEGALATRGPSLFD
HHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCHHHH
VLLTATPRQADR
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]